A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate
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Shan, J.; Toye, P.G. 2009. A novel immuno-polymerase chain reaction protocol incorporating a highly purified streptavidin-DNA conjugate. Journal of Immunoassay and Immunochemistry 30:322-337.
Permanent link to this item: http://hdl.handle.net/10568/1494
We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immuno-PCR is reproducible, with a larger dynamic range and a sensitivity several orders of magnitude greater than the corresponding conventional ELISA. The minimum concentration of analyte detected was of the order of 1 pg/mL These characteristics should contribute to the adoption of immuno-PCR by research and clinical laboratories.
Philip Toye is ILRI author