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    Recombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures

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    Authors
    Kitani, H.
    Black, S.J.
    Nakamura, Y.
    Naessens, Jan
    Murphy, N.
    Yokomizo, Y.
    Gibson, John P.
    Iraqi, F.A.
    Date
    2002-04
    Language
    en
    Type
    Journal Article
    Accessibility
    Limited Access
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    Citation
    Kitani, H.; Black, S.J.; Nakamura, Y.; Naessens, J.; Murphy, N.; Yokomizo, Y.; Gibson, J.; Iraqi, F. 2002. Recombinant tumor necrosis factor alpha does not inhibit the growth of African trypanosomes in Axenic cultures. Infection and Immunology 70(4):2210-2214.
    Permanent link to cite or share this item: https://hdl.handle.net/10568/1540
    DOI: https://dx.doi.org/10.1128/IAI.70.4.2210-2214.2002
    Abstract/Description
    Mice whose tumor necrosis factor alpha (TNF-) genes were disrupted developed higher levels of parasitemia than wild-type mice following infection with Trypanosoma congolense IL1180 or T. brucei brucei GUTat3.1, confirming the results of earlier studies. To determine whether TNF- directly affects the growth of these and other bloodstream forms of African trypanosomes, we studied the effects of recombinant mouse, human, and bovine TNF- on the growth of two isolates of T. congolense, IL1180 and IL3338, and two isolates of T. brucei brucei, GUTat3.1 and ILTat1.1, under axenic culture conditions. The preparations of recombinant TNF- used were biologically active as determined by their capacity to kill L929 cells. Of five recombinant TNF- lots tested, one lot of mouse TNF- inhibited the growth of both isolates of T. brucei brucei and one lot of bovine TNF- inhibited the growth of T. brucei brucei ILTat1.1 but only at very high concentrations and without causing detectable killing of the parasites. The other lots of mouse recombinant TNF-, as well as human TNF-, did not affect the growth of any of the test trypanosomes even at maximal concentrations that could be attained in the culture systems (3,000 to 15,000 U of TNF-/ml of medium). These results suggest that exogenously added recombinant TNF- generally does not inhibit the growth of African trypanosomes under the culture conditions we used. The impact of TNF- on trypanosome parasitemia may be indirect, at least with respect to the four strains of trypanosomes reported here.
    AGROVOC Keywords
    TRYPANOSOMA CONGOLENSE; ANIMAL DISEASES
    Subjects
    ANIMAL DISEASES; GENETICS; LIVESTOCK;
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    • ILRI animal bioscience program outputs [207]
    • ILRI articles in journals [5077]

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