Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells
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Lambson, B., Nene, V., Obura, M., Shah, T., Pandit, P., Ole-Moiyoi, O., Delroux, K., Welburn, S., Skilton, R., Villiers, E.P. de and Bishop, R. 2005. Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells. Insect Molecular Biology 14: 403-414.
Permanent link to this item: http://hdl.handle.net/10568/21174
Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs had significant identity (< 1e-15) to previously identified sequences. Polypeptides that may interact with host pathways included a kinase inhibitor. Two proteins encoded homologues of the yolk protein vitellogenin and seventeen contained glycine-rich motifs. Four proteins without sequence matches had conserved structural folds, identified using a Threading algorithm. Predicted secretion signals were between fifteen and fifty-seven amino acids long. Four homologous polymorphic proteins contained conserved (26/27 residues) signal peptides. Ten functional tick secretion signals could not be unambiguously identified using predictive algorithms.