CGSpaceA Repository of Agricultural Research Outputs
    View Item 
    •   CGSpace Home
    • International Livestock Research Institute (ILRI)
    • ILRI articles in journals
    • View Item
       
    • CGSpace Home
    • International Livestock Research Institute (ILRI)
    • ILRI articles in journals
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Study on single nucleotide polymorphisms of H -FABP gene in sheep

    Thumbnail
    
    Authors
    Qiao, H.Y.
    Zhao, Q.J.
    Yao, N.
    Lu, Y.-Z.
    Meng, X.-R.
    Guan, Wei-Jun
    Ma, Y.-H.
    Han Jianlin
    Date
    2009-08
    Language
    zh
    Type
    Journal Article
    Accessibility
    Limited Access
    Metadata
    Show full item record
    Share
    Citation
    Qiao, H.-Y., Zhao, Q.-J., Yao, N., Lu, Y.-Z., Meng, X.-R., Jianlin, H., Guan, W.-J.; Ma, Y.-H. 2009. Study on single nucleotide polymorphisms of H -FABP gene in sheep. Hereditas (Beijing) 31(7):725-731.
    Permanent link to cite or share this item: http://hdl.handle.net/10568/2168
    DOI: https://dx.doi.org/10.3724/SP.J.1005.2009.00725
    Abstract/Description
    PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48), Ningxia Tan sheep (Tan, 121), Tan × SH F1 (23), Poll Dorset (48) and Suffolk (24) sheep were screened in this stuty. The result showed: (1)four SNPs at 981(G/A), 1014(A/C) 1019(T/C) and 1058 (-/G) and nine genotypes (AA, BB, CC, AB, AC, BC, AD, CD and BD) were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content (PIC) in the Suffolk and Tan × SH F1 populations (PIC< 0.25) but an intermediate PIC in the remaining three populations (0.25< PIC <0.50). It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. (2)Three genotypes (HH, Hh and hh) determined by a SNP at 2407(T-C) were detected using primer 4. The genotype frequencies were in the order of HH>Hh>hh. A chi-square analy-sis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low (PIC < 0.25). However, there was no polymorphisms in SH, Tan × SH F1 and Suffolk populations.
    AGROVOC Keywords
    GENETICS; SHEEP
    Subjects
    LIVESTOCK; GENETICS;
    Countries
    CHINA
    Regions
    ASIA
    Collections
    • ILRI articles in journals [4825]
    • CAAS-ILRI Joint Laboratory outputs [9]
    • ILRI livestock genetics program outputs [291]

    AboutSend Feedback
     

    My Account

    LoginRegister

    Browse

    All of CGSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesBy AGROVOC keywordBy ILRI subjectBy CPWF subjectBy CCAFS subjectBy CIFOR subjectBy IWMI subjectBy RegionBy CountryBy SubregionBy CRP subjectBy River basinBy Output typeBy CTA subjectBy WLE subjectBy Bioversity subjectBy CIAT subjectBy CIP subjectBy animal breedBy CGIAR System subjectThis CollectionBy Issue DateAuthorsTitlesBy AGROVOC keywordBy ILRI subjectBy CPWF subjectBy CCAFS subjectBy CIFOR subjectBy IWMI subjectBy RegionBy CountryBy SubregionBy CRP subjectBy River basinBy Output typeBy CTA subjectBy WLE subjectBy Bioversity subjectBy CIAT subjectBy CIP subjectBy animal breedBy CGIAR System subject

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    AboutSend Feedback