Strong position-dependent effects of sequence mismatches on signal ratios measured using long oligonucleotide microarrays
MetadataShow full item record
Rennie, C.; Noyes, H.A.; Kemp, S.J.; Hulme, H.; Brass, A.; Hoyle, D.C. 2008. Strong position-dependent effects of sequence mismatches on signal ratios measured using long oligonucleotide microarrays. BMC Genomics 9:317.
Permanent link to cite or share this item: http://hdl.handle.net/10568/2202
Microarrays are an important and widely used tool. Applications include capturing genomic DNA for high-throughput sequencing in addition to the traditional monitoring of gene expression and identifying DNA copy number variations. Sequence mismatches between probe and target strands are known to affect the stability of the probe-target duplex, and hence the strength of the observed signals from microarrays.