CGSpaceA Repository of Agricultural Research Outputs
    View Item 
    •   CGSpace Home
    • International Livestock Research Institute (ILRI)
    • ILRI archive
    • View Item
       
    • CGSpace Home
    • International Livestock Research Institute (ILRI)
    • ILRI archive
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa

    Thumbnail
    
    Authors
    Morzaria, S.P.
    Katende, J.
    Musoke, A.J.
    Nene, Vishvanath
    Skilton, R.A.
    Bishop, Richard
    Date
    1999
    Language
    en
    Type
    Journal Article
    Accessibility
    Limited Access
    Metadata
    Show full item record
    Share
    Citation
    Parasitologia;41(suppl. 1): 73-80
    Permanent link to cite or share this item: http://hdl.handle.net/10568/29383
    Abstract/Description
    Tick-borne diseases (TBDs) are a major economic constraint to livestock production in sub-Saharan Africa. ILRI is focussing on developing a range of products, such as vaccines, diagnostics and decision support services to underpin improved control programmes against these diseases. We have developed three highly sensitive and specific enzyme linked immuno-assays (ELISAs), which allow precise diagnosis of Theileria parva, Babesia bigemina and Anaplasma marginale. These tests have been standardised and validated using defined experimental and field infection sera. Parasite specific recombinant antigens and monoclonal antibodies against bovine immunoglobulins as secondary antibodies have played an important role in enhancing the sensitivity and specificity of the assays. They have been further evaluated in on-farm longitudinal sero-epidemiological studies to define infection dynamics and disease risks in various farming systems in Kenya and Uganda. In addition, DNA-based tests for differentiation of Theileria species and characterisation of Theileria parva stocks have been developed. These tests have been derived through physical mapping and sequencing of key elements of the T. parva genome, which include repetitive and telomeric regions, minisatellite sequences, antigen genes and a number of random DNA sequences. These tools are currently being deployed in conjunction with field immunisation programmes to determine the biological impact of introducing live vaccines of T. parva on population dynamics.
    AGROVOC Keywords
    TICK BORNE DISEASES; CATTLE; AFRICA; BABESIA; THEILERIA; ANAPLASMA; ELISA; PCR; DIAGNOSIS; GENETIC VARIATION; IMMUNODIAGNOSIS; VACCINES; ANTIGENS
    Subjects
    CATTLE; VACCINES; ANIMAL DISEASES;
    Regions
    AFRICA
    Collections
    • ILRI articles in journals [4825]
    • ILRI archive [4979]

    AboutSend Feedback
     

    My Account

    LoginRegister

    Browse

    All of CGSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesBy AGROVOC keywordBy ILRI subjectBy CPWF subjectBy CCAFS subjectBy CIFOR subjectBy IWMI subjectBy RegionBy CountryBy SubregionBy CRP subjectBy River basinBy Output typeBy CTA subjectBy WLE subjectBy Bioversity subjectBy CIAT subjectBy CIP subjectBy animal breedBy CGIAR System subjectThis CollectionBy Issue DateAuthorsTitlesBy AGROVOC keywordBy ILRI subjectBy CPWF subjectBy CCAFS subjectBy CIFOR subjectBy IWMI subjectBy RegionBy CountryBy SubregionBy CRP subjectBy River basinBy Output typeBy CTA subjectBy WLE subjectBy Bioversity subjectBy CIAT subjectBy CIP subjectBy animal breedBy CGIAR System subject

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    AboutSend Feedback