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dc.contributor.authorVoigt, W.P.
dc.contributor.authorMwaura, S.N.
dc.contributor.authorNjihia, G.M.
dc.contributor.authorNyaga, S.G.
dc.contributor.authorYoung, A.S.
dc.date.accessioned2013-06-11T09:23:41Z
dc.date.available2013-06-11T09:23:41Z
dc.date.issued1995
dc.identifier.citationParasitology Research;81(1): 74-81
dc.identifier.issn0932-0113
dc.identifier.urihttps://hdl.handle.net/10568/29471
dc.description.abstractA comparison of ten methods for staining tick salivary glands for detection of Theileria parva infection from ticks fed on rabbits for various periods was under taken. Staining with azure without hydrochloric acid hydrolysis was found to be the most reliable method for detection of the presporozoite stage (sporoblasts) of T.parva in the salivary gland acini of unfed Rhipicephalus appendiculatus and could be applied to field ticks. All the stains proved suitable for the detection and quantitation of sporozoites in ticks fed for 4 days on rabbits. The capacity of the stains to allow detection of early stages of T. parva differed, but it became more reliable during tick feeding as sporoblasts developed and matured. Giemsa's stain and Feulgen's stain followed by superimposition of Giemsa's stain were superior to other stains for the detection and quantitation of immature salivary gland stages in feeding ticks.
dc.language.isoen
dc.sourceParasitology Research
dc.subjectTHEILERIA PARVA
dc.subjectSALIVARY GLANDS
dc.subjectRHIPICEPHALUS APPENDICULATUS
dc.subjectANIMAL DISEASES
dc.subjectMETASTIGMATA
dc.titleDetection of Theileria parva in the salivary glands of Rhipicephalus appendiculatus: Evaluation of staining methods
dc.typeJournal Article
cg.subject.ilriANIMAL DISEASES
cg.subject.ilriDISEASE CONTROL
cg.subject.ilriLIVESTOCK
cg.identifier.statusLimited Access
cg.identifier.doihttps://dx.doi.org/10.1007/BF00932420


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