Characterisation of a monoclonal antibody recognising the CD3 epsilon chain of the bovine T cell receptor complex
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Veterinary Immunology and Immunopathology;61(1): 25-35
Permanent link to this item: http://hdl.handle.net/10568/29861
The characterisation of a monoclonal antibody (mAb), designated MM1A, which reacts with an antigen molecule on the surface of bovine alfa beta and gama delta TcR+ T cells is described. The mAb immunoprecipitated a series of polypeptides of 21 kDa, 22 kDa, 36 kDa and 44 kDa which is consistent with it recognising the TcR/CD3 complex. COS cells, transfected with a cDNA encoding the bovine CD3e chain, reacted with mAb MM1A indicating that the epitope recognised is on the chain of the complex and confirming the mAb recognised bovine CD3.