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    Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers

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    Authors
    Yada, B.
    Tukamuhabwa, P.
    Wanjala, B.W.
    Kim, D.J.
    Skilton, Robert A.
    Alajo, B.A.
    Mwanga, R.O.M.
    Date
    2010-02
    Language
    en
    Type
    Journal Article
    Accessibility
    Limited Access
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    Citation
    Yada, B., Tukamuhabwa, P., Wanjala, B., Kim, D.J., Skilton, R.A., Alajo, B.A. and Mwanga, R.O.M. 2010. Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers. HortScience 45(2):225-230.
    Permanent link to cite or share this item: https://hdl.handle.net/10568/3480
    External link to download this item: http://hortsci.ashspublications.org/cgi/content/abstract/45/2/225
    Abstract/Description
    The genetic relationships among 192 superior, high-yielding, and disease-resistant sweetpotato [Ipomoea batatas (L.) Lam] accessions from the Ugandan germplasm collection were analyzed using 10 fluorescent labeled simple sequence repeat (SSR) markers. Relatedness among the genotypes was estimated using the Nei and Li genetic distance coefficient, cluster analysis and principle component analysis methods of NTSYS-pc software. The polymorphic information content of the SSR markers used in this study ranged from 0.23 to 0.76 for loci IB-S07 and [B-R12, respectively, with a mean value of 0.62. The number of polymorphic alleles detected per locus ranged from two to six with a mean of four, a confirmation of the effectiveness of microsatellite detection on an automated ABI 3730 sequencer. The mean pairwise genetic distance among the 192 genotypes was 0.57, an indication of moderately high genetic diversity. Cluster analysis divided the accessions into four major groups with no relationship to the district of origin. Two sets of duplicates were identified through SSR genotyping in this study. Up to 190 distinct accessions for use as potential parental genotypes in hybridization schemes for cultivar development in the region were identified.
    Subjects
    BIODIVERSITY; CROPS; RESEARCH;
    Countries
    UGANDA
    Regions
    AFRICA; EAST AFRICA
    Collections
    • CIP Journal Articles [616]
    • ILRI BecA articles [169]
    • ILRI articles in journals [5079]

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