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    Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease

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    Authors
    Kovacs, G.
    Sagi, L.
    Jacon, G.
    Arinaitwe, G.
    Busogoro, J.P.
    Thiry, E.
    Strosse, H.
    Swennen, R.L.
    Remy, S.
    Date
    2013
    Language
    en
    Type
    Journal Article
    Review status
    Peer-reviewed
    Accessibility
    Open Access
    Metadata
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    Citation
    Kovacs, G.; Sagi, L.; Jacon, G.; Arinaitwe, G.; Busogoro, J.P.; Thiry, E.; Strosse, H.; Swennen, R.; Remy, S. -2013-Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease-Transgenic Research 22(1)-p. 117-130
    Permanent link to cite or share this item: https://hdl.handle.net/10568/35582
    DOI: https://dx.doi.org/10.1007/s11248-012-9631-1
    Abstract/Description
    Transgenic banana (Musa acuminata 'Gros Michel') integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt selectable marker gene in more than 90 % of the lines tested, whereas more than three quarters of the lines contained the linked rice chitinase gene resulting in a co-transformation frequency of at least 71.4 %. Further, a unique stable integration of the transgenes in each line revealed some false negative PCR results and the expected co-transformation frequency of 100 %.
    CGIAR Affiliations
    Roots, Tubers and Bananas
    AGROVOC Keywords
    MUSA (BANANAS); MYCOSPHAERELLA FIJIENSIS; PLANT DISEASES; TRANSGENIC PLANTS; TRANSGENICS
    Subjects
    PLANT DISEASES; TRANSGENIC PLANTS; TRANSGENICS;
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    • Bioversity Journal Articles [806]

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