Cryopreservation of redwood (Sequoia sempervirens (D. Don.) Endl.) in vitro buds using vitrification-based techniques.
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Ozudogru, E.A.; Kirdok, E.; Kaya, E.; Capuana, M.; Benelli, C.; Engelmann, F. -2011-Cryopreservation of redwood (Sequoia sempervirens (D. Don.) Endl.) in vitro buds using vitrification-based techniques. -CryoLetters 32-p. 99-110
Permanent link to this item: http://hdl.handle.net/10568/35754
In this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation-vitrification and droplet-vitrification were compared for cryopreserving Sequoia sempervirens apical and basal buds sampled from in vitro shoot cultures. The effect of cold-hardening of mother-plants and of bud culture medium and sucrose preculture was also investigated. Culture of apical and basal buds sampled from cold-hardened mother-plants on Quoirin and Lepoivre medium with activated charcoal had a positive effect on regrowth. Only droplet-vitrification ensured survival and regrowth after cryopreservation. After cryopreservation, regeneration of apical buds was possible for PVS2 exposure durations between 90 and 180 min but it remained low, with a maximum of 18% after 135 min treatment. With basal buds, regeneration after cryopreservation was possible over a larger range of PVS2 treatment durations, between 30 and 180 min. The highest regeneration percentage was slightly higher (22%) than that measured with apical buds, and was also achieved after 135 min PVS2 exposure.