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dc.contributor.authorArango, R.E.
dc.contributor.authorTogawa, R.C.
dc.contributor.authorCarpentier, S.C.
dc.contributor.authorRoux, N.
dc.contributor.authorHekkert, B.L.
dc.contributor.authorKema, G.H.J.
dc.contributor.authorSouza, M. de
dc.date.accessioned2014-06-10T08:26:15Z
dc.date.available2014-06-10T08:26:15Z
dc.date.issued2011
dc.identifier.citationArango, R.E.; Togawa, R.C.; Carpentier, S.C.; Roux, N.; Hekkert, B.L.; Kema, G.H.J.; De Souza, M. -2011-Genome-wide BAC-end sequencing of Musa acuminata DH Pahang reveals further insights into the genome organization of banana-Tree Genetics & Genomes 7 (5)-p. 933-940
dc.identifier.issn1614-2942
dc.identifier.urihttp://hdl.handle.net/10568/35767
dc.description.abstractBanana and plantain ( Musa spp.) are grown in more than 120 countries in tropical and subtropical regions and constitute an important staple food for millions of people. A Musa acuminata ssp. malaccencis DH Pahang bacterial artificial chromosome (BAC) library (MAMB) was submitted for BAC-end sequencing. MAMB consists of 23,040 clones, with a 140-kbp average insert size, accounting for a five times coverage of the banana genome. A total of 46,080 reads were generated, and 42,750 (92.8%) high-quality sequences were obtained after trimming for vector and quality. Analysis of these data shows a GC content of 41.39%, whereas interspersed repeats comprise 32.3%. The most common repeated sequences found show homology to ribosomal RNA genes, particularly 18S rRNA, while the Ty3/gypsy type monkey retrotransposon is the most common retro element. The sequence data were used to generate a banana-specific repeat library containing 54 new repetitive elements which accounted for 11.86% of the total nucleotides. Simple sequence repeats represent 0.7% of the sequence data and allowed the identification of 2,455 potentially useful marker sites. Functional annotation identified 2,705 sequences that could code for proteins of known function. Microsynteny analysis shows a higher number of co-linear matches to Oryza sativa , in contrast to Arabidopsis thaliana . This database of BAC-end sequences is useful for the assembly of the complete banana genome sequence and is important for identification in functional genomics experiments.
dc.format.extentp. 933-940
dc.language.isoen
dc.sourceTree Genetics & Genomes
dc.subjectGENOMES
dc.subjectMUSA
dc.subjectMUSA ACUMINATA
dc.titleGenome-wide BAC-end sequencing of Musa acuminata DH Pahang reveals further insights into the genome organization of banana
dc.description.versionPeer-reviewed
dc.typeJournal Article
cg.subject.bioversityGENOMES
cg.identifier.statusLimited Access
cg.contributor.affiliationBioversity International
cg.contributor.affiliationCorporacion para Investigaciones Biologicas
cg.contributor.affiliationUniversidad Nacional de Colombia
cg.contributor.affiliationEmpresa Brasileira de Pesquisa Agropecuaria
cg.contributor.affiliationKatholieke Universiteit, Leuven
cg.identifier.doihttps://dx.doi.org/10.1007/s11295-011-0385-3
cg.isijournalISI Journal


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