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Fki, L.; Bouaziz, N.; Sahnoun, N.; Swennen, R.; Drira, N.; Panis B. -2011-Palm cryobanking-Cryoletters 32 (6)-p. 451-462
Permanent link to this item: http://hdl.handle.net/10568/35773
We describe the development of an efficient cryopreservation protocol for proembryogenic masses (PEMs) of date palm variety 'Barhee'. Proembryos were induced by inoculating small pieces of juvenile leaves on MS medium supplemented with 0.3 mg.L-1 2,4- D. Application of these in vitro conditions led to true-to-type plants as observed after plant fructification. When compared to the standard vitrification protocol, the ultra-rapid dropletvitrification technique proved to be superior. Sucrose preculture considerably increased postcryopreservation recovery. The highest regeneration after cryogenic exposure reached 63.3% when PEMs were treated with PVS2 for 30 min at 0Ã‚Â°C and 56.7% when PVS2 treatment lasted for 15 min at 25Ã‚Â°C. The first signs of regrowth of cryopreserved PEMs were observed after 2 to 3 weeks. Cryopreservation did not affect the morphogenetic capacities of the plant material. Moreover, highly proliferating suspension cultures could be established from the cryopreserved material. The overall production of somatic embryos from 500 mg cryopreserved PEMs reached 1030 Ãƒâ€šÃ‚Â± 50 units after 1 month. The morphological study of date palms regenerated from cryopreserved material confirmed the stability of clonal material following cryopreservation.