Development of alternative plant vitrification solutions in droplet vitrification procedures
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Kim, H.H.; Lee, Y.G.; Shin, D.J.; Ko, H.C.; Gwag, J.G.; Cho, E.G.I.; Engelmann, F. -2011-Development of alternative plant vitrification solutions in droplet vitrification procedures -ISHS 908-p. 181-186
Permanent link to cite or share this item: http://hdl.handle.net/10568/42399
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Twelve alternative plant vitrification solutions (PVSs) were designed by modifying cryoprotectant concentrations taken from the original PVS2 and PVS3. These solutions were tested in the droplet-vitrification procedure on apices of two model species, garlic and chrysanthemum. The results revealed that PVS3 and its variants were superior to PVS2 and PVS2-based solutions in terms of regrowth while most PVS2 variants were comparable to the original PVS2. The lower effectiveness of PVS2-based solutions was attributed to their cytotoxicity as well as to insufficient protection of samples from freeze-thaw events revealed by the differential scanning calorimetry (DSC). Both garlic and chrysanthemum apices were sensitive to chemical toxicity caused by permeating cryoprotectants. Chrysanthemum apices were also sensitive to osmotic stress. The results suggest that PVS2-based PVSs with increased glycerol and sucrose and/or decreased DMSO and EG concentrations can be applied for small or medium size explants which are tolerant to chemical toxicity and moderately sensitive to osmotic stress. PVS3 and variants can be used widely when samples are heterogeneous, of large size and/or very sensitive to chemical toxicity and tolerant to osmotic stress.