Ultrastructural changes in suspension cultures of banana (Musa spp.) during cryopreservation by vitrification
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Xu,C.; Li, Y.; Deng, X.; Chen, H.; Panis, B. -2011-Ultrastructural changes in suspension cultures of banana (Musa spp.) during cryopreservation by vitrification -ISHS 908-p. 73-81
Permanent link to this item: http://hdl.handle.net/10568/42407
Internet URL: http://www.actahort.org/books/908/908_6.htm
Using transmission electron microscopy, ultrastructural changes taking place during the application of a vitrification protocol of embryogenic cells of 'Beida Aijiao' were studied. The results showed that the control cells contained a lot of organelles, a regular nucleolus envelope and intact plasma membranes. After treatment with 25% PVS2, some changes could be observed, such as smaller vacuoles, more phenolic compounds, swollen organelles and appearance of many lipid bodies. Also multi- membranous structures were observed and many vesicles appeared between the plasma membrane and the cell wall. Moreover, the phenomenon of plasmolysis remained limited. Only after dehydration with 100% PVS2 did plasmolysis became more severe. Nucleus envelopes in these cells were malformed. Though the cytoplasm and the nucleus became more electricity-dense and a lot of heterochromatin appeared, the plasma membrane was still intact. The ultrastructure of cells after freezing, thawing and unloading was similar to that of those cells after dehydration. After 2 weeks of post-thaw recovery, the ultrastructure of survival cells was similar to that of the control cells.