Cryopreservation of avocado embryogenic cultures
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Guzman, E.; Bradai, F.; Sanchez-Romero, C.; Panis, B. -2011-Cryopreservation of avocado embryogenic cultures -ISHS 908-p. 215-226
Permanent link to this item: http://hdl.handle.net/10568/42408
Internet URL: http://www.actahort.org/books/908/908_26.htm
The establishment of an efficient cryopreservation protocol for avocado embryogenic cultures is considered of outermost importance in order to avoid problems related to long-term in vitro maintenance such as risk of contamination, somaclonal variation or loss of embryogenic competence. Three cryopreservation protocols were compared: a classical vitrification-based protocol, an ultra-fast freezing method using droplet vitrification on aluminium foil strips and a slow freezing method (-1°C/min). Post-thaw survival (i.e explants resuming growth 5 weeks after thawing). was genotype-dependent, but in general, higher frequencies of regrowth were obtained when using vitrification-based protocols. The type of embryogenic tissue used as cryopreservation explants had a significant influence on survival. When using embryogenic callus or somatic embryos at their early developmental stage all explants resumed growth five weeks after thawing.