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    Detection and characterization of a phytoplasma associated with frog skin disease in cassava

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    Authors
    Alvarez, E.
    Mejia, JF
    Llano, Germán, A.
    Loke, JB
    Date
    2007
    Language
    en
    Type
    Journal Article
    Review status
    Peer-reviewed
    Accessibility
    Open Access
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    Permanent link to cite or share this item: http://hdl.handle.net/10568/43321
    External link to download this item: http://www.bulletinofinsectology.org/pdfarticles/vol60-2007-273-274alvarez.pdf
    Abstract/Description
    Cassava is one of the main carbohydrate sources in the tropics, and it has worldwide importance as basic food for millions of persons in America and Africa. This root crop is affected by cassava frog skin disease that in Colombia produces yield losses of almost 90%. A phytoplasma was associated with this disease by using a nested-PCR followed by RFLP analyses, cloning and sequencing of 16S ribosomal gene. Sequence analysis showed that the phytoplasma was related to those belonging to 16SrIII ribosomal group, with a 99% sequence homology. Evidence of phytoplasma aetiology for the disease was achieved also by electron microscopy observations, grafting to specific host plants, and by its dodder transmission. Symptoms were reproduced in healthy plants. Symptoms disappearance in leaves was obtained by growing cassava infected plantlets in a chloroxytetracycline solution producing further evidence for the phytoplasma aetiology. This study is the first to report a phytoplasma associated with cassava frog skin disease.
    AGROVOC Keywords
    MANIHOT ESCULENTA; PLANT DISEASES; PHYTOPLASMAS; IDENTIFICATION; PCR; MANIHOT ESCULENTA; ENFERMEDADES DE LAS PLANTAS; FITOPLASMAS; IDENTIFICACIÓN; PCR
    Subjects
    CASSAVA; PESTS AND DISEASES;
    Countries
    COLOMBIA
    Regions
    SOUTH AMERICA
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    • CIAT Articles in Journals [2249]

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