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    Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola

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    Authors
    Mahuku, G.S.
    Henriquez Naranjo, MA
    Munoz, J
    Buruchara, Robin A.
    Date
    2002
    Language
    en
    Type
    Journal Article
    Review status
    Peer-reviewed
    Accessibility
    Open Access
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    Permanent link to cite or share this item: https://hdl.handle.net/10568/43878
    DOI: https://dx.doi.org/10.1094/PHYTO.2002.92.6.580
    Abstract/Description
    Coevolution of the angular leaf spot pathogen, Phaeoisariopsis griseola, with its common bean host has been demonstrated, and P. griseola isolates have been divided into Andean and Mesoamerican groups that correspond to defined bean gene pools. Recent characterization of P. griseola isolates from Africa has identified a group of isolates classified as Andean using random amplified polymorphic DNA (RAPD), but which are able to infect some Mesoamerican differential varieties. These isolates, designated Afro-Andean, have been identified only in Africa. Random amplified microsatellites, RAPD, and restriction digestion of amplified ribosomal intergenic spacer region were used to elucidate the relationships among the Afro-Andean, Andean, and Mesoamerican groups of P. griseola. Cluster and multiple correspondence analysis of molecular data separated isolates into Andean and Meso-american groups, and the Afro-Andean isolates clustered with Andean isolates. Analysis of molecular variance ascribed 2.8% of the total genetic variation to differences between Afro-Andean and Andean isolates from Africa. Gene diversity analysis revealed no genetic differentiation (GST = 0.004) between Afro-Andean and Andean isolates from Africa. However, significant levels of genetic differentiation (GST = 0.39) were observed between Afro-Andean or Andean isolates from Africa and Andean isolates from Latin America, revealing significant geographical differentiation within the Andean lineage. Results from this study showed that Afro-Andean isolates do not constitute a new P. griseola group and do not represent long-term evolution of the pathogen genome, but rather are likely the consequents of point mutations in genes for virulence. This finding has significant implications in the deployment of resistant bean genotypes.
    CGIAR Author ORCID iDs
    Robin Burucharahttps://orcid.org/0000-0003-0934-1218
    AGROVOC Keywords
    PHASEOLUS VULGARIS; PHAEOISARIOPSIS GRISEOLA; GENETIC VARIATION; GENETIC MARKERS; PCR; PROVENANCE; PHASEOLUS VULGARIS; PHAEOISARIOPSIS GRISEOLA; VARIACIÓN GENÉTICA; MARCADORES GENÉTICOS; PCR; PROCEDENCIA
    Subjects
    BEANS; BIODIVERSITY; PESTS AND DISEASES;
    Regions
    AFRICA; SOUTH AMERICA
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    • CIAT Articles in Journals [2395]

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