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dc.contributor.authorMahuku, G.S.
dc.contributor.authorHenriquez Naranjo, MA
dc.contributor.authorMunoz, J
dc.contributor.authorBuruchara, Robin A.
dc.date.accessioned2014-10-02T08:32:52Z
dc.date.available2014-10-02T08:32:52Z
dc.date.issued2002
dc.identifier.issn0031-949X
dc.identifier.urihttps://hdl.handle.net/10568/43878
dc.description.abstractCoevolution of the angular leaf spot pathogen, Phaeoisariopsis griseola, with its common bean host has been demonstrated, and P. griseola isolates have been divided into Andean and Mesoamerican groups that correspond to defined bean gene pools. Recent characterization of P. griseola isolates from Africa has identified a group of isolates classified as Andean using random amplified polymorphic DNA (RAPD), but which are able to infect some Mesoamerican differential varieties. These isolates, designated Afro-Andean, have been identified only in Africa. Random amplified microsatellites, RAPD, and restriction digestion of amplified ribosomal intergenic spacer region were used to elucidate the relationships among the Afro-Andean, Andean, and Mesoamerican groups of P. griseola. Cluster and multiple correspondence analysis of molecular data separated isolates into Andean and Meso-american groups, and the Afro-Andean isolates clustered with Andean isolates. Analysis of molecular variance ascribed 2.8% of the total genetic variation to differences between Afro-Andean and Andean isolates from Africa. Gene diversity analysis revealed no genetic differentiation (GST = 0.004) between Afro-Andean and Andean isolates from Africa. However, significant levels of genetic differentiation (GST = 0.39) were observed between Afro-Andean or Andean isolates from Africa and Andean isolates from Latin America, revealing significant geographical differentiation within the Andean lineage. Results from this study showed that Afro-Andean isolates do not constitute a new P. griseola group and do not represent long-term evolution of the pathogen genome, but rather are likely the consequents of point mutations in genes for virulence. This finding has significant implications in the deployment of resistant bean genotypes.
dc.language.isoen
dc.sourcePhytopathology
dc.subjectPHASEOLUS VULGARIS
dc.subjectPHAEOISARIOPSIS GRISEOLA
dc.subjectGENETIC VARIATION
dc.subjectGENETIC MARKERS
dc.subjectPCR
dc.subjectPROVENANCE
dc.subjectPHASEOLUS VULGARIS
dc.subjectPHAEOISARIOPSIS GRISEOLA
dc.subjectVARIACIÓN GENÉTICA
dc.subjectMARCADORES GENÉTICOS
dc.subjectPCR
dc.subjectPROCEDENCIA
dc.titleMolecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola
dc.description.versionPeer-reviewed
dc.typeJournal Article
cg.subject.ciatBEANS
cg.subject.ciatBIODIVERSITY
cg.subject.ciatPESTS AND DISEASES
cg.identifier.statusOpen Access
cg.identifier.doihttps://dx.doi.org/10.1094/PHYTO.2002.92.6.580
cg.isijournalISI Journal
cg.coverage.regionAFRICA
cg.coverage.regionSOUTH AMERICA
cg.creator.idRobin Buruchara: 0000-0003-0934-1218


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