The cassava genome: current progress, future directions
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Prochnik, Simon; Marri, Pradeep Reddy; Desany, Brian; Rabinowitch, Pablo D.; Kodira, Chinnappa; Fauquet, Claude M.; Tohme, Joseph M.; Rodríguez, Fausto; Mohiuddin, Mohammed; Harkins, Timothy; Rokhsar, Daniel S.; Rounsley, Steve. 2012. The cassava genome: current progress, future directions . Tropical Plant Biology 5: 88-94
Permanent link to this item: http://hdl.handle.net/10568/44154
The starchy swollen roots of cassava provide an essential food source for nearly a billion people, as well as possibilities for bioenergy, yet improvements to nutritional content and resistance to threatening diseases are currently impeded. A 454-based whole genome shotgun sequence has been assembled, which covers 69% of the predicted genome size and 96% of protein-coding gene space, with genome finishing underway. The predicted 30,666 genes and 3,485 alternate splice forms are supported by 1.4 M expressed sequence tags (ESTs). Maps based on simple sequence repeat (SSR)-, and EST-derived single nucleotide polymorphisms (SNPs) already exist. Thanks to the genome sequence, a high-density linkage map is currently being developed from a cross between two diverse cassava cultivars: one susceptible to cassava brown streak disease; the other resistant. An efficient genotyping-by-sequencing (GBS) approach is being developed to catalog SNPs both within the mapping population and among diverse African farmer-preferred varieties of cassava. These resources will accelerate marker-assisted breeding programs, allowing improvements in disease-resistance and nutrition, and will help us understand the genetic basis for disease resistance.