CIP and tissue culture
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CTA. 1988. CIP and tissue culture. Spore 13. CTA, Wageningen, The Netherlands.
Permanent link to this item: http://hdl.handle.net/10568/44800
The International Potato Center (CIP) has had several major successes in the field of tissue culture through collaborative research with many other institutions. In genetic engineering, CIP has worked with the Louisiana State University in the USA...
The International Potato Center (CIP) has had several major successes in the field of tissue culture through collaborative research with many other institutions. In genetic engineering, CIP has worked with the Louisiana State University in the USA to insert synthetic genes into potato plants. Using Agrobacterium plasmid vectors to introduce a synthetic gene, the scientists have been able to enhance the nutritional value of the potato by obtaining the supplementary production of a synthetic protein rich in essential amino acids. This is produced from a synthetic gene that can be synthesised in the laboratory proving that the technology now exists to engineer genes into the potato genetically. Considerable research and some appropriate legislation will still be needed, however, before the genetically engineered plants can be released to national potato programmes. Based on this success, work is now being directed towards genetically introducing resistance to pests and diseases. In particular, scientists hope to develop clones with resistance to Erwinia and Pseudomonas bacterial infections. They believe it will also be possible to introduce a gene that disrupts viral replication giving resistance to potato spindle tube viroid and potato leafroll virus. Collaboration with the Weizmann Institute in Israel has led to successes in the area of protoplant fusion, the technique that has been used to transfer cytoplasmic male sterility in tobacco. Work to apply this technique is now in progress, and this will allow hybrid true potato seed to be produced without having to emasculate female plants Other work, in collaboration with organizations across Europe, is looking at the factors that limit some types of tissue culture regeneration - the process whereby haploid plants have been generated from pollen grains. Improvements in this area will greatly assist ClP's routine tissue culture work, and will also improve levels of genetic stability during tissue culture and the pathogen elimination work that relies heavily on tissue culture. Source: CIP Circular Volume 15 No. 1 March 1987 For more details, contact: CIP P O Box 5969 Lima PERU