Tackling bacterial wilt in potatoes
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CTA. 1990. Tackling bacterial wilt in potatoes. Spore 27. CTA, Wageningen, The Netherlands.
Permanent link to this item: http://hdl.handle.net/10568/45291
Researchers at the Burundi Institute of Agricultural Sciences (ISABU) have devised a simple in vitro laboratory to beat the problem of bacterial wilt in potato seed production. Problems with bacterial wilt in seed stock had severely limited potato...
Researchers at the Burundi Institute of Agricultural Sciences (ISABU) have devised a simple in vitro laboratory to beat the problem of bacterial wilt in potato seed production. Problems with bacterial wilt in seed stock had severely limited potato production in Burundi. The main problem was found to be disease latent in stock-seed plants grown above 2000m. The disease only became apparent when grown at lower elevations, adding to the already well-established problems brought about by poor cultural practices in the fields and the use of infected seed tubers. In l987 a scheme was devised to use in vitro pathogen-tested plantlets from locally selected varieties as initial stock for propagation through single node cuttings. Before dissemination to the farmers, the plantlets were grown in the field by conventional propagation techniques. These new procedures required a fairly sophisticated laboratory but, when there was not time to get the equipment for this in vitro work, ISABU researchers developed their own 'laminar flow cabinet'. This is a plastic covered wooden frame which they use successfully as a sterile transfer chamber for plantlets. Lighted shelves serve as the culture area. The only other equipment required is razor blades, scissors, tweezers, petri plates and other standard laboratory items. Tubers derived from these in vitro plantlets have now been multiplied under field conditions and, coupled with good cultural practices, this new, healthy, Improved stock-seed will be instrumental in reducing the occurrence of bacterial wilt. It will also realise ISABU's goal of putting potato production in Burundi back on schedule. ISNAR Laan van Nieuw Oost Indie 133 2593 EM The Hague THE NETHERLANDS