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dc.contributor.authorAmimo, J.O.
dc.contributor.authorJunga, J.O.
dc.contributor.authorOgara, W.O.
dc.contributor.authorVlasova, A.N.
dc.contributor.authorNjahira, Moses N.
dc.contributor.authorMaina, S.
dc.contributor.authorOkoth, Edward A.
dc.contributor.authorBishop, Richard P.
dc.contributor.authorSaif, L.J.
dc.contributor.authorDjikeng, Appolinaire
dc.date.accessioned2014-12-17T07:18:10Z
dc.date.available2014-12-17T07:18:10Z
dc.date.issued2015-02-25
dc.identifier.citationAmimo, J.O., Junga, J.O., Ogara, W.O., Vlasova, A.N., Njahira, M.N., Maina, S., Okoth, E.A., Bishop, R.P., Saif, L.J. and Djikeng, A. 2015. Detection and genetic characterization of porcine group A rotaviruses in asymptomatic pigs in smallholder farms in East Africa: Predominance of P[8] genotype resembling human strains. Veterinary Microbiology 175(2-4):195–210.en_US
dc.identifier.urihttps://hdl.handle.net/10568/52240
dc.description.abstractViral enteritis is a serious problem accounting for deaths in neonatal animals and humans worldwide. The absence of surveillance programs and diagnostic laboratory facilities have resulted in a lack of data on rotavirus associated diarrheas in pigs in East Africa. Here we describe the incidence of group A rotavirus (RVA) infections in asymptomatic young pigs in East Africa. Of the 446 samples examined, 26.2% (117/446) were positive for RVA. More nursing piglets (78.7%) shed RVA than weaned (32.9%) and grower (5.8%) pigs. RVA incidence was higher in pigs that were either housed_free-range (77.8%) or tethered_free-range (29.0%) than those that were free-range or housed or housed-tethered pigs. The farms with larger herd size (>10 pigs) had higher RVA prevalence (56.5%) than farms with smaller herd size (24.1-29.7%). This study revealed that age, management system and pig density significantly (p < 0.01) influenced the incidence of RVA infections, with housed_free-range management system and larger herd size showing higher risks for RVA infection. Partial (811-1604nt region) sequence of the VP4 gene of selected positive samples revealed that different genotypes (P[6], P[8] and P[13]) are circulating in the study area with P[8] being predominant. The P[6] strain shared nucleotide (nt) and amino acid (aa) sequence identity of 84.4-91.3% and 95.1-96.9%, respectively, with known porcine and human P[6] strains. The P[8] strains shared high nt and aa sequence identity with known human P[8] strains ranging from 95.6-100% and 92-100%, respectively. The P[13] strains shared nt and aa sequence identity of 83.6-91.7% and 89.3-96.4%, respectively, only with known porcine P[13] strains. No P[8] strains yielded RNA of sufficient quality/quantity for full genome sequencing. However analysis of the full genome constellation of the P[6], two P[13] and one untypeable strains revealed that the P[6] strain (Ke-003-5) genome constellation was G26-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1, P[13] strains (Ug-049 and Ug-453) had G5-P[13]-I5-R1-C1-M1-A8-N1-T7-E1-H1 while the untypeable strain (Ug-218) had G5-P[?]-I5-R1-C1-M1-A8-N1-T1-E1-H?. In conclusion, P[6] and P[8] genotypes detected were genetically closely related to human strains suggesting the possibility of interspecies transmission. Further studies are required to determine the role of RVA in swine enteric disease burden and to determine the genetic/antigenic heterogeneity of the circulating strains for development of accurate diagnostic tools and to implement appropriate prophylaxis programs.en_US
dc.language.isoenen_US
dc.sourceVeterinary Microbiologyen_US
dc.subjectSWINEen_US
dc.subjectGENETICSen_US
dc.subjectANIMAL DISEASESen_US
dc.subjectVACCINATIONen_US
dc.titleDetection and genetic characterization of porcine group A rotaviruses in asymptomatic pigs in smallholder farms in East Africa: Predominance of P[8] genotype resembling human strainsen_US
dc.typeJournal Articleen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriANIMAL HEALTHen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriGENETICSen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriPIGSen_US
cg.subject.ilriVACCINESen_US
cg.identifier.statusLimited Accessen_US
cg.contributor.affiliationUniversity of Nairobi
cg.contributor.affiliationInternational Livestock Research Institute
cg.identifier.doihttps://dx.doi.org/10.1016/j.vetmic.2014.11.027en_US
cg.isijournalISI Journalen_US
cg.coverage.regionAFRICAen_US
cg.coverage.regionEAST AFRICAen_US


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