Agentes gelatinizadores en el cultivo de tejidos
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SZABADOS, L.; Nunez, V.M; Tello, L.M.; MAFLA, G.; ROA, J.; ROCA, W.M. 1991. Agentes gelatinizadores en el cultivo de tejidos. In: Roca, William M.; Mroginski, Luis A. (eds.). Cultivo de tejidos en la agricultura: Fundamentos y aplicaciones. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. p. 79-93.
Permanent link to this item: http://hdl.handle.net/10568/55093
The effect of several gelling agents (bacto-agar at 0.7 and 0.8 percent, the latter purified with 3 consecutive washings in distilled water; agarose; gelrite; liquid medium, with a cotton or filter paper support) on the successful development of the following tissue and cell cultures: the initiation of meristem culture, micropropagation, callus survival and growth, regeneration of callus derived from anther culture, and plating efficiency in cell suspension cultures and in protoplast-derived colonies. In the case of cassava meristem culture, highly significant differences were found in fresh wt. increase of the resulting shoots; solidification with agarose, gelrite, and liquid medium was better than with agar. When cassava bud apexes were micropropagated using nodal cuttings, significant differences were found in shoot fresh wt. and length. Gelrite and agarose stimulated culture growth better. When the formation of somatic embryos was induced in immature cassava leaves, no significant differences were observed among the different gelling agents. Gelrite (Kelco Div. of Merck & Co.) was found to be an effective gelling agent that solidifies tissue culture media in all systems tested and species studied. (CIAT)