Changes in sugar content and proteome of potato in response to cold and dehydration stress and their implications for cryopreservation
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Folgado, R.; Sergeant, K.; Renaut, J.; Swennen, R.; Hausman, J.F.; Panis, B. -2014-Changes in sugar content and proteome of potato in response to cold and dehydration stress and their implications for cryopreservation-Journal of Proteomics 98-p. 99-111
Permanent link to this item: http://hdl.handle.net/10568/66071
The key to successful cryopreservation lies in the induction of tolerance towards dehydration/desiccation and freezing. The accumulation of osmo-active compounds, which can be induced by drought and cold stress, is therefore important. In the present study, three-week old shoots from in vitro plantlets of the cultivated potato Solanum tuberosum and its frost-resistant relative Solanum commersonii were submitted to osmotic stress (by using sucrose) and chilling (6 °C). After 14 days of exposure, shoot tips were sampled in order to gain an insight into changes of the proteome and soluble sugars. Also, the effect of these treatments on growth performance behaviour and on the success of cryopreservation was evaluated. Identified proteins that changed in abundance due to stress were associated with stress response. Additionally, carbohydrate analyses in both species, after exposure to chilling, also indicated species-related differences; this observation could point towards a better-adapted physiological state of the donor plants of S. commersonii prior to the cryoprocedure and therefore a better recovery of the meristems.