CGSpaceA Repository of Agricultural Research Outputs
    View Item 
    •   CGSpace Home
    • International Livestock Research Institute (ILRI)
    • ILRI Programs
    • Biosciences eastern and central Africa (BecA) ILRI Hub
    • ILRI BecA articles
    • View Item
       
    • CGSpace Home
    • International Livestock Research Institute (ILRI)
    • ILRI Programs
    • Biosciences eastern and central Africa (BecA) ILRI Hub
    • ILRI BecA articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    A database of expressed genes From Cochliomyia hominivorax (Diptera: Calliphoridae)

    Thumbnail
    
    Authors
    Guerrero, F.D.
    Dowd, S.E.
    Djikeng, Appolinaire
    Wiley, G.
    MacMillan, Susan
    Saldivar, L.
    Najar, F.
    Roe, B.A.
    Date
    2009
    Language
    en
    Type
    Journal Article
    Accessibility
    Limited Access
    Metadata
    Show full item record
    Share
    Citation
    Guerrero, F.D., Dowd, S.E., Djikeng, A., Wiley, G., Macmil, S., Saldivar, L., Najar, F. and Roe, B.A. 2009. A database of expressed genes from Cochliomyia hominivorax (Diptera: Calliphoridae). Journal of Medical Entomology 46(5): 1109-1116.
    Permanent link to cite or share this item: http://hdl.handle.net/10568/68336
    DOI: https://dx.doi.org/10.1603/033.046.0518
    Abstract/Description
    We used an expressed sequence tag and 454 pyrosequencing approach to initiate a study of the genome of the screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). Two normalized cDNA libraries were constructed from RNA isolated from embryos and second instar larvae from the Panama 95 strain. Approximately 5,400 clones from each library were sequenced from both the 5? and 3? directions using the Sanger method. In addition, double-stranded cDNA was prepared from random-primed polyA RNA purified from embryos, second-instar larvae, adult males, and adult females. These four cDNA samples were used for 454 pyrosequencing that produced ?300,000 independent sequences. Sequences were assembled into a database of assembled contigs and singletons and used to search public protein databases and annotate the sequences. The full database consists of 6,076 contigs and 58,221 singletons assembled from both the traditional expressed sequence tag (EST) and 454 sequences. Annotation of the data led to the identification of several gene coding regions with possible roles in sex determination in the screwworm. This database will facilitate the design of microarray and other experiments to study screwworm gene expression on a larger scale than previously possible.
    AGROVOC Keywords
    ANIMAL BREEDING; GENETICS
    Subjects
    DATA; GENETICS; ANIMAL BREEDING;
    Collections
    • ILRI BecA articles [142]

    AboutSend Feedback
     

    My Account

    LoginRegister

    Browse

    All of CGSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesBy AGROVOC keywordBy ILRI subjectBy CPWF subjectBy CCAFS subjectBy CIFOR subjectBy IWMI subjectBy RegionBy CountryBy SubregionBy CRP subjectBy River basinBy Output typeBy CTA subjectBy WLE subjectBy Bioversity subjectBy CIAT subjectBy CIP subjectBy animal breedBy CGIAR System subjectThis CollectionBy Issue DateAuthorsTitlesBy AGROVOC keywordBy ILRI subjectBy CPWF subjectBy CCAFS subjectBy CIFOR subjectBy IWMI subjectBy RegionBy CountryBy SubregionBy CRP subjectBy River basinBy Output typeBy CTA subjectBy WLE subjectBy Bioversity subjectBy CIAT subjectBy CIP subjectBy animal breedBy CGIAR System subject

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    AboutSend Feedback