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dc.contributor.authorGujaria-Verma, Neha
dc.contributor.authorRamsay, Larissa
dc.contributor.authorSharpe, Andrew G.
dc.contributor.authorSanderson, Lacey-Anne
dc.contributor.authorDebouck, Daniel G.
dc.contributor.authorTar’an, Bunyamin
dc.contributor.authorBett, Kirstin E.
dc.date.accessioned2016-03-22T20:55:18Z
dc.date.available2016-03-22T20:55:18Z
dc.date.issued2016-03-15
dc.identifier.citationGujaria-Verma, Neha; Ramsay, Larissa; Sharpe, Andrew G.; Sanderson, Lacey-Anne; Debouck, Daniel G.; Tar’an, Bunyamin; Bett, Kirstin E. 2016. Gene-based SNP discovery in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris) for diversity analysis and comparative mapping. BMC Genomics 17:239.en_US
dc.identifier.issn1471-2164
dc.identifier.urihttps://hdl.handle.net/10568/72712
dc.description.abstractBackground Common bean (Phaseolus vulgaris) is an important grain legume and there has been a recent resurgence in interest in its relative, tepary bean (P. acutifolius), owing to this species’ ability to better withstand abiotic stresses. Genomic resources are scarce for this minor crop species and a better knowledge of the genome-level relationship between these two species would facilitate improvement in both. High-throughput genotyping has facilitated large-scale single nucleotide polymorphism (SNP) identification leading to the development of molecular markers with associated sequence information that can be used to place them in the context of a full genome assembly. Results Transcript-based SNPs were identified from six common bean and two tepary bean accessions and a subset were used to generate a 768-SNP Illumina GoldenGate assay for each species. The tepary bean assay was used to assess diversity in wild and cultivated tepary bean and to generate the first gene-based map of the tepary bean genome. Genotypic analyses of the diversity panel showed a clear separation between domesticated and cultivated tepary beans, two distinct groups within the domesticated types, and P. parvifolius was confirmed to be distinct. The genetic map of tepary bean was compared to the common bean genome assembly to demonstrate high levels of collinearity between the two species with differences limited to a few intra-chromosomal rearrangements. Conclusions The development of the first set of genomic resources specifically for tepary bean has allowed for greater insight into the structure of this species and its relationship to its agriculturally more prominent relative, common bean. These resources will be helpful in the development of efficient breeding strategies for both species and will facilitate the introgression of agriculturally important traits from one crop into the other.en_US
dc.format.extent17:239en_US
dc.language.isoenen_US
dc.sourceBMC Genomicsen_US
dc.subjectPHASEOLUS VULGARISen_US
dc.subjectGENETIC MARKERSen_US
dc.subjectPOLYMORPHISMen_US
dc.subjectGENOMESen_US
dc.subjectGENETIC RESOURCESen_US
dc.subjectPLANT BREEDINGen_US
dc.subjectMARCADORES GENÉTICOSen_US
dc.subjectRECURSOS GENÉTICOSen_US
dc.subjectPOLIMORFISMOen_US
dc.subjectGENOMASen_US
dc.subjectFITOMEJORAMIENTOen_US
dc.titleGene-based SNP discovery in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris) for diversity analysis and comparative mappingen_US
dc.description.versionPeer Reviewen_US
dc.typeJournal Articleen_US
cg.subject.ciatBEANSen_US
cg.subject.ciatGENETIC RESOURCESen_US
cg.subject.ciatPLANT BREEDINGen_US
cg.identifier.statusOpen Accessen_US
cg.contributor.affiliationUniversity of Saskatchewanen_US
cg.contributor.affiliationNational Research Council Canadaen_US
cg.contributor.affiliationInternational Center for Tropical Agricultureen_US
cg.identifier.doihttps://dx.doi.org/10.1186/s12864-016-2499-3en_US
cg.isijournalISI Journalen_US


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