Show simple item record

dc.contributor.authorSaini, H.
dc.contributor.authorKashihara, Y.
dc.contributor.authorLópez-Montes, A.
dc.contributor.authorAsiedu, R.
dc.date.accessioned2016-08-09T12:28:34Z
dc.date.available2016-08-09T12:28:34Z
dc.date.issued2016-06-22
dc.identifier.citationSaini, H., Kashihara, Y., Lopez-Montes, A. & Asiedu, R. (2016). Interspecific Crossing between Yam Species (Dioscorea rotundata and Dioscorea bulbifera) through in Vitro Ovule Culture. American Journal of Plant Sciences, 7(8), 1268-1274en_US
dc.identifier.issn2158-2742
dc.identifier.urihttps://hdl.handle.net/10568/76358
dc.description.abstractIn the present study, in vitro ovule culture technique was used to obtain interspecific cross combination of Dioscorea rotundata ufenyi and Dioscorea bulbifera wild. Ten days after pollination, ovules were excised and cultured onto 1/2 strength Murashige and Skoog (MS) medium (Basal salt mixture + Vitamins) supplemented with 6% sucrose, 0.7% agar and plant growth hormones such as GA3, BAP, Picrolam and TDZ. Cultured ovules were transferred on 1/2 MS medium with 3% sucrose and 0.7% agar after three weeks. 40 days after pollination, germination was observed from 7 months cultured ovule between D. rotundata ufenyi x D. bulbifera wild. Hybridity of the regenerated plant was checked by flow cytometric method. A close relation was observed between the fluorescence intensity of the obtained progeny with one of the parents’ fluorescence. The observed progeny can be closely correlated with an apomictic tissue from an ovule parent of D. rotundata ufenyi. Plantlets derived from ovule culture were proliferated through in vitro shoot multiplication with hormonal concentration (0.5 mg/l BAP) supplemented with 1/2 strength MS medium. Obtained ovule culture derived in vitro plantlets were successfully hardened, acclimatized and transferred to the field, where they survived and grew normally. In plant breeding, interspecific crossing is very important technique, enabling the time needed to produce homozygous lines to be shortened as compared to the conventional plant breeding techniques.en_US
dc.format.extent1268-1274en_US
dc.language.isoenen_US
dc.sourceAmerican Journal of Plant Sciencesen_US
dc.subjectIN VITROen_US
dc.subjectDIOSCOREA ROTUNDATAen_US
dc.subjectDIOSCOREA BULBIFERAen_US
dc.titleInterspecific crossing between yam species (Dioscorea rotundata and Dioscorea bulbifera) through in vitro ovule cultureen_US
dc.description.versionPeer Reviewen_US
dc.typeJournal Articleen_US
cg.authorship.typesCGIAR single centreen_US
cg.subject.iitaYAMen_US
cg.identifier.statusOpen Accessen_US
cg.contributor.affiliationInternational Institute of Tropical Agricultureen_US
cg.targetaudienceSCIENTISTSen_US
cg.fulltextstatusFormally Publisheden_US
cg.identifier.doihttps://dx.doi.org/10.4236/ajps.2016.78122en_US
cg.coverage.regionAFRICAen_US
cg.coverage.regionWEST AFRICAen_US
cg.coverage.countryNIGERIAen_US
cg.contributor.crpRoots, Tubers and Bananasen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record