Analysis of karyotypes and evolutionary features of wild species of buckwheat
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Shi Jian-qiang; Li Yan-qin; Zhang Zong-wen; Wu Bin; Wang An-hu (2016) Analysis of karyotypes and evolutionary features of wild species of buckwheat. Journal of Plant Genetic Resources 2016-03. ISSN: 1672-1810
Permanent link to this item: http://hdl.handle.net/10568/77336
Buckwheat was originated in southwest of China,where exists a large number of wild relatives. Wild buckwheat karyotype feature analysis has important implications for buckwheat breeding practices. In this study,F. esculentum spp. ancestralis,F. urophyllum,F. leptopodum var. grossii,F. gracilipes,F. gracilipes var. odontopterum were selected as experimental materials and karyotypes of these species were identified by squash method. The results showed that F. esculentum spp. ancestralis,F. urophyllum and F. leptopodum var. grossii were all diploid,the karyotype formula were 2n = 2x= 16 = 12 M + 4m( 2SAT),2n = 2x = 16 = 16 M,2n = 2x = 16 = 14 M + 2m( 2SAT),while that of F. gracilipes and F. gracilipes var. odontopterum were tetraploid,and the karyotype formula were 2n = 4x = 32 = 32 M and 2n = 4x = 32= 30 M + 2m( 2SAT). Karyotype genus type F. esculentum spp. ancestralis and F. urophyllum was 1A,and the rests were1 B. F. esculentum spp. ancestralis,F. leptopodum var. grossii,F. gracilipes var. odontopterum had one pair of satellite chromosomes. It proved that basic chromosome number of wild species of buckwheat was 8. There were diploid and tetraploid levels in wild buckwheat. The analysis indicated that F. urophyllum had a primitive evolutionarily status,while F.gracilipes var. odontopterum was a more advanced tetraploid buckwheat wild species in evolutionary status.