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dc.contributor.authorTamiru, M.
dc.contributor.authorNatsume, S.
dc.contributor.authorTakagi, H.
dc.contributor.authorWhite, B.
dc.contributor.authorYaegashi, H.
dc.contributor.authorShimizu, M.
dc.contributor.authorYoshida, K.
dc.contributor.authorUemura, A.
dc.contributor.authorOikawa, K.
dc.contributor.authorAbe, A.
dc.contributor.authorUrasaki, N.
dc.contributor.authorMatsumura, H
dc.contributor.authorBabil, P.
dc.contributor.authorYamanaka, S.
dc.contributor.authorMatsumoto, R.
dc.contributor.authorMuranaka, S.
dc.contributor.authorGirma, G.
dc.contributor.authorLópez-Montes, A.
dc.contributor.authorGedil, M.
dc.contributor.authorBhattacharjee, R.
dc.contributor.authorAbberton, M.
dc.contributor.authorKumar, P.L.
dc.contributor.authorRabbi, Ismail Y
dc.contributor.authorTsujimura, M.
dc.contributor.authorTerachi, T.
dc.contributor.authorHaerty, W.
dc.contributor.authorCorpas, M.
dc.contributor.authorKamoun, S.
dc.contributor.authorKahl, G.
dc.contributor.authorTakagi, H.
dc.contributor.authorAsiedu, R.
dc.contributor.authorTerauchi, R
dc.date.accessioned2017-12-13T13:21:32Z
dc.date.available2017-12-13T13:21:32Z
dc.date.issued2017
dc.identifier.citationTamiru, M., Natsume, S., Takagi, H., White, B., Yaegashi, H., Shimizu, M., ... & Terauchi, R. (2017). Genome sequencing of the staple food crop white Guinea yam enables the development of a molecular marker for sex determination. BMC Biology, 15(86), 1-20.en_US
dc.identifier.issn1741-7007
dc.identifier.urihttp://hdl.handle.net/10568/89726
dc.descriptionOpen Access Journal; Published online: 19 Sept 2017en_US
dc.description.abstractBackground Root and tuber crops are a major food source in tropical Africa. Among these crops are several species in the monocotyledonous genus Dioscorea collectively known as yam, a staple tuber crop that contributes enormously to the subsistence and socio-cultural lives of millions of people, principally in West and Central Africa. Yam cultivation is constrained by several factors, and yam can be considered a neglected “orphan” crop that would benefit from crop improvement efforts. However, the lack of genetic and genomic tools has impeded the improvement of this staple crop. Results To accelerate marker-assisted breeding of yam, we performed genome analysis of white Guinea yam (Dioscorea rotundata) and assembled a 594-Mb genome, 76.4% of which was distributed among 21 linkage groups. In total, we predicted 26,198 genes. Phylogenetic analyses with 2381 conserved genes revealed that Dioscorea is a unique lineage of monocotyledons distinct from the Poales (rice), Arecales (palm), and Zingiberales (banana). The entire Dioscorea genus is characterized by the occurrence of separate male and female plants (dioecy), a feature that has limited efficient yam breeding. To infer the genetics of sex determination, we performed whole-genome resequencing of bulked segregants (quantitative trait locus sequencing [QTL-seq]) in F1 progeny segregating for male and female plants and identified a genomic region associated with female heterogametic (male = ZZ, female = ZW) sex determination. We further delineated the W locus and used it to develop a molecular marker for sex identification of Guinea yam plants at the seedling stage. Conclusions Guinea yam belongs to a unique and highly differentiated clade of monocotyledons. The genome analyses and sex-linked marker development performed in this study should greatly accelerate marker-assisted breeding of Guinea yam. In addition, our QTL-seq approach can be utilized in genetic studies of other outcrossing crops and organisms with highly heterozygous genomes. Genomic analysis of orphan crops such as yam promotes efforts to improve food security and the sustainability of tropical agriculture.en_US
dc.description.sponsorshipJapan International Research Center for Agricultural Sciencesen_US
dc.description.sponsorshipInternational Institute of Tropical Agricultureen_US
dc.format.extent1-20en_US
dc.language.isoenen_US
dc.sourceBMC Biologyen_US
dc.subjectYAMen_US
dc.subjectDIOSCOREAen_US
dc.subjectSEX DETERMINATIONen_US
dc.subjectFOOD SECURITYen_US
dc.subjectGENOMEen_US
dc.subjectMOLECULAR MARKERen_US
dc.subjectGUINEA YAMen_US
dc.subjectWHOLE-GENOME SEQUENCEen_US
dc.subjectDIOECYen_US
dc.titleGenome sequencing of the staple food crop white Guinea yam enables the development of a molecular marker for sex determinationen_US
dc.description.versionPeer Reviewen_US
dc.typeJournal Articleen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.subject.iitaGENETIC IMPROVEMENTen_US
cg.subject.iitaPLANT GENETIC RESOURCESen_US
cg.subject.iitaYAMen_US
cg.identifier.statusOpen Accessen_US
cg.contributor.affiliationIwate Biotechnology Research Center, Japanen_US
cg.contributor.affiliationEarlham Instituteen_US
cg.contributor.affiliationKobe Universityen_US
cg.contributor.affiliationOkinawa Agricultural Research Centeren_US
cg.contributor.affiliationShinshu Universityen_US
cg.contributor.affiliationTokyo University of Agricultureen_US
cg.contributor.affiliationJapan International Research Center for Agricultural Sciencesen_US
cg.contributor.affiliationInternational Institute of Tropical Agricultureen_US
cg.contributor.affiliationKyoto Sangyo Universityen_US
cg.contributor.affiliationSainsbury Laboratory, UKen_US
cg.contributor.affiliationUniversity of Frankfurten_US
cg.targetaudienceSCIENTISTSen_US
cg.fulltextstatusFormally Publisheden_US
cg.identifier.doihttp://dx.doi.org/10.1186/s12915-017-0419-xen_US
cg.isijournalISI Journalen_US
cg.coverage.regionAFRICAen_US
cg.coverage.regionASIAen_US
cg.coverage.regionEAST ASIAen_US
cg.coverage.regionWEST AFRICAen_US
cg.coverage.countryJAPANen_US
cg.coverage.countryNIGERIAen_US
cg.contributor.crpGrain Legumesen_US
cg.contributor.crpMaizeen_US
cg.contributor.crpRoots, Tubers and Bananasen_US


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