Estimation of the nuclear DNA content in some representative of genus Dioscorea
Date Issued
2009Language
enType
Journal ArticleReview status
Peer ReviewAccessibility
Open AccessMetadata
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Obidiegwu, J.E., Rodriguez, E., Ene-Obong, E.E., Loureiro, J., Muoneke, C.O., Santos, C., ... & Asiedu, R. (2009). Estimation of the nuclear DNA content in some representative of genus Dioscorea. Scientific Research and Essays, 4(5), 448-452.
Permanent link to cite or share this item: https://hdl.handle.net/10568/90219
Abstract/Description
Nuclear DNA content of 54 accessions of 6 Dioscorea species collected from West African countries were determined using flow cytometry. Observed ploidy levels are diploid (2x), triploid (3x), tetraploid (4x), hexaploid (6x) and octoploid (8x). DNA index varied from 0.36 to 1.54 depending on the cultivar and considered specie. Estimated nuclear DNA sizes ranged from 0.702 ± 0.004 pg for G1 nuclei of diploid Dioscorea dumetorum to 2.573 ± 0.020 pg for G1 nuclei of octoploid Dioscorea cayenensis. Our result showed that there is a decrease in genome size with higher ploidy level. The study contributes a step towards a genome understanding of this group of plants
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Subjects
YAM; GENETIC IMPROVEMENTOrganizations Affiliated to the Authors
International Institute of Tropical Agriculture; National Root Crops Research Institute, Nigeria; Michael Okpara University of Agriculture; University of Aveiro; Tun Abdul Razak Research CentreInvestors/sponsors
Kirkhouse TrustCollections
- IITA Journal Articles [4999]