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dc.contributor.authorLi, M.
dc.contributor.authorZhang, Z.
dc.contributor.authorWu, B.
dc.contributor.authorGao, J.
dc.contributor.authorLi, Y.
dc.date.accessioned2018-01-24T10:13:32Z
dc.date.available2018-01-24T10:13:32Z
dc.date.issued2017
dc.identifier.citationLi, M.; Zhank, Z.; Wu, B.; Gao, J.; Li, Y. (2017) Genetic diversity of tartary buckwheat based on the sequence analysis of PAL. Journal of Plant Genetic Resources, 18(3) p. 530-537 ISSN: 1672-1810en_US
dc.identifier.issn1672-1810
dc.identifier.urihttps://hdl.handle.net/10568/90565
dc.description.abstractTartary buckwheat is widely cultivated in different areas of China and gradually evolved into genetic diversity. In order to study and utilize the tartary buckwheat, the PAL gene differences of the tartary buckwheat were analyzed on 67 samples,which were collected from different provinces of China( including the north region and the southwest region) and the Nepal region. We amplified the PAL gene by PCR and then acquired the sequence results.Based on the sequences,genetic diversity was analyzed and cluster of analysis of 67 samples was also carried out by NJ method. The results showed that after aligning and splicing, the length the PAL sequence was 2011 bp in the matrix,the variable sites was 160,while the parsimony informative sites was 33,accounting for 7. 9% and 1. 64% of the total length, respectively. Variable type were mainly base transition and base transversions. Variable site mainly concentrated in the region of 600-1200,which was the site of the N-terminal of the exon-2. The intra-specific mean distance of the Sichuan group was 0. 016,while the tartary buckwheat from Inner Mongolia group showed that the intra-specific mean distance was 0. 002. And it revealed that there was a significant difference among the tartary buckwheat resources in genetics diversity from different geographic origins. We checked the 67 PAL sequences using software. And it showed that the average value of the Nucleotide polymorphism( p) and the average heterozygosity( ?) were 0. 0034 and 0. 0143, respectively. The Nucleotide polymorphism( p) value in samples from Sichuan was the highest( 0. 0148) . From the phylogenetic tree, it indicated that the 67 samples from the different province could be clustered into seven categories. And the 5 sample in Tibet were clustered into another category. These results showed that the PAL sequences of the majority tartary buckwheat samples was stable and the differences between the most samples was not significant. The samples in Sichuan province had abundant genetic diversity. But only in samples from Tibet it revealed many SNPs site,and this should be the new mutation spots in the PAL coding regions.en_US
dc.format.extentp. 530-537en_US
dc.language.isozhen_US
dc.publisherIngenta Connecten_US
dc.sourceJournal of Plant Genetic Resources, 18(3)en_US
dc.subjectFAGOPYRUM AGOPYRUMen_US
dc.subjectGENE EXPRESSIONen_US
dc.subjectGENETIC DIVERSITY AS RESOURCESen_US
dc.subjectGENETIC VARIATIONen_US
dc.titleGenetic diversity of tartary buckwheat based on the sequence analysis of PALen_US
dc.typeJournal Articleen_US
cg.subject.bioversityGENETIC DIVERSITY AS RESOURCES
cg.link.permalinkhttps://www.bioversityinternational.org/index.php?id=244&tx_news_pi1%5Bnews%5D=9524en_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.identifier.statusOpen Accessen_US
cg.contributor.affiliationShanxi Universityen_US
cg.contributor.affiliationChinese Academy of Agricultural Sciencesen_US
cg.contributor.affiliationBioversity Internationalen_US
cg.targetaudienceSCIENTISTSen_US
cg.identifier.urlhttps://www.bioversityinternational.org/index.php?id=244&tx_news_pi1%5Bnews%5D=9524en_US
cg.identifier.doihttps://dx.doi.org/10.13430/j.cnki.jpgr.2017.03.018en_US
cg.coverage.regionASIAen_US


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