Banana nectar as a medium for testing pollen viability and germination in Musa
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Nyine, M. & Pillay, M. (2007). Banana nectar as a medium for testing pollen viability and germination in Musa. African Journal of Biotechnology, 6(10), 1175-1180.
Permanent link to cite or share this item: http://hdl.handle.net/10568/91445
A quick and reliable method for evaluating pollen quality is essential in a breeding program, especially in a crop such as banana that is characterized by high male and female sterility. In this study the germination and viability of banana pollen was evaluated in a sucrose solution and diluted banana nectar. Twenty banana accessions were used to evaluate pollen germination in the two media after 3 and 24 h. Nineteen genotypes (95%) showed significantly higher pollen germination potential (PGP) in diluted nectar than in 3% sucrose solution. The accession TMB2x 8075 - 7 showed no significant pollen germination in nectar and sucrose. Eleven genotypes (55%) showed significant increase in pollen germination by increasing the time of incubation whereas pollen germination for nine genotypes (45%) was not affected by increase in incubation time. Nectar from different banana clones influenced pollen germination suggesting a genotype effect for pollen germination in Musa.
Investors/sponsorsDirectorate General for International Co-operation, Belgium
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