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dc.contributor.authorAbdurahman, A.
dc.contributor.authorGriffin, D.
dc.contributor.authorElphinstone, J.
dc.contributor.authorStruik, P.C.
dc.contributor.authorSchulz, S.
dc.contributor.authorSchulte-Geldermann, E.
dc.contributor.authorSharma, K.
dc.identifier.citationAbdurahman, A.; Griffin, D.; Elphinstone, J.; Struik, P.C.; Schulz, S.; Schulte-Geldermann, E.; Sharma, K. 2017. Molecular characterization of Ralstonia solanacearum strains from Ethiopia and tracing potential source of bacterial wilt disease outbreak in seed potatoes. Plant Pathology. (UK). ISSN 0032-0862. 66(5):826-834.en_US
dc.description.abstractBacterial wilt, caused by Ralstonia solanacearum, is emerging as a major threat to potato production in Ethiopia, reaching epidemic proportions in the Chencha district recently, with a prevalence of 97% of potato fields in 2015. The recent disease outbreak in the district coincided with a significant introduction of seed potatoes. This research was therefore initiated to genetically characterize the pathogen so as to trace its source, identify its relationship with outbreaks in the rest of the country, and make intervention recommendations. Ralstonia solanacearum isolates were sampled both from seed and ware potato fields in Chencha and from seed potato fields in production regions suspected of being potential sources of the pathogen. Multiplex PCR and phylogenetic analysis of partial endoglucanase gene sequences identified all of the isolates as phylotype IIB sequevar 1. VNTR sequence analysis distinguished 11 different haplotypes, nine of which were unique to the Chencha district. However, one of the haplotypes was common to all seed potato producer regions of Ethiopia except for the Shashemene area. The unique and diverse VNTR haplotypes of the pathogen in Chencha indicates that it is well established in the district. When a geographical map of the VNTR haplotypes was superimposed with the main cross-regional seed potato distribution pattern of the country, it became evident that the pathogen was being disseminated via latently infected seed from the Holeta-Jeldu area in the Central Highlands of Ethiopia. Identification of largely uninfected highland districts and multiplication of high grade seed potato exclusively in those districts should be given priority.en_US
dc.description.sponsorshipUnited States Agency for International Developmenten_US
dc.description.sponsorshipWalsh Fellowship of Teagasc-Irelanden_US
dc.description.sponsorshipWageningen Universityen_US
dc.sourcePlant Pathologyen_US
dc.subjectSEED POTATOESen_US
dc.titleMolecular characterization of Ralstonia solanacearum strains from Ethiopia and tracing potential source of bacterial wilt disease outbreak in seed potatoesen_US
dc.description.versionPeer Reviewen_US
dc.typeJournal Articleen_US
cg.authorship.typesCGIAR single centreen_US
cg.identifier.statusOpen Accessen_US
cg.contributor.affiliationWageningen University and Research Centreen_US
cg.contributor.affiliationDepartment of Crop Science, Irelanden_US
cg.contributor.affiliationFera Science Ltd.en_US
cg.contributor.affiliationDeutsche Gesellschaft für Internationale Zusammenarbeiten_US
cg.contributor.affiliationInternational Potato Centeren_US
cg.fulltextstatusFormally Publisheden_US
cg.isijournalISI Journalen_US
cg.coverage.regionEAST AFRICAen_US
cg.contributor.crpRoots, Tubers and Bananasen_US

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