1 ASSESMENT OF POULTRY PRODUCTIONCONSTRAINTS AND EVALUATION OF DIFFERENT CONCENTRATE ON PRODUCTION PERFORMANCE AND PARASITIC BURDEN OF EXOTIC CHICKENS IN KERSA DISTRICT OF JIMMA ZONE MSc Thesis By Gezali Abafaji Major advisor: Hailu Degefu (DVM, MVSc, Associate Professor of Epidemiology) Co-advisor: Professor Solomon Demeke Co-advisor: Dr. Barbara Wieland MAY 2017 JIMMA, ETHIOPIA 2 ASSESMENT OF POULTRY PRODUCTIONCONSTRAINTS AND EVALUATION OF DIFFERENT CONCENTRATE ON PRODUCTION PERFORMANCE AND PARASITIC BURDEN OF EXOTIC CHICKENS IN KERSA DISTRICT OF JIMMA ZONE By Gezali Abafaji A THESIS SUBMITTED TO THE SCHOOL OF GRADUATE STUDIES OF JIMMA UNIVERSITY COLLEGE OF AGRICULTURE AND VETERINARY MEDICINE IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE MASTERES DEGREE IN VETERINARY EPIDEMIOLOGY MAY 2017 JIMMA, ETHIOPIA i ii Dedication I dedicate this MSc Thesis work to my father Abafaji Abafita and my mother W/ro Lubaba Abanaem for all the love they have given me, their dedicated partnership in the success of my life and for their prayers and support. iii STATEMENT OF AUTHOR First, I declare that this Thesis is my original work and all sources or materials used for this Thesis have fully acknowledged. This thesis having been submitted in partial fulfillment of the requirements for MSc degree in Veterinary Epidemiologyat Jimma University College of Agriculture and Veterinary Medicine and is deposited in the University Library to be made available under the rules of the library. I declare that this thesis not submitted to any other institutions anywhere for the award of any academic degree, diploma or certificate. Brief quotations from this Thesis are allowable without special permission if the source accurately acknowledged. Requests for permission for comprehensive citation from, duplicate of this manuscript in whole, or in part may grant by the School of Veterinary Medicine and or the School of Graduate Studies of Jimma University. In all other instances, however, permission should obtain from the author. Name: Gezali Abafaji Abafita Signature: _______________________ Place: Jimma University, Jimma Date of submission: ______________ iv BIOGRAPHICAL SKETCH The author, Gezali Abafaji, was born on 6November 1989 in Kersa District, Jimma Zone of Oromia National Regional State. He attended his elementary education (grade 1-6) at Dibu Bujit Elementary School from 1995-2000, grade 7 and 8 at Serbo secondary school from 2001-2002, grade 9 and 10 at Serboo high school from 2003-2004. Preparatory at jimma preparatory school from 2005-2006. Dr. Gezali has received DVM from JUCAVM in June 2011.Dr. Gezali Abafaji had worked for Dedo District Animal Health and DevelopmentOffice in Jimma Zone, Southwestern Ethiopia as an expert for 2 years. v Acknowledgment I give all glory and honour to the Almighty God whose guidance and provision made this work to succeed. I express my sincere gratitude to the University of Jimma and livestock and irrigation value chain for Ethiopian small holder (LIVES) project for their financial and material support. Special thanks go to my supervisors Dr. Hailu Degefu, Professor Solomon Demeke and Dr. Barbara Wieland for their guidance and continuous help. May the Almighty God richly reward them for the work; well done. My sincere gratitude goes to Dr. Gemeda of the LIVES project focal person for his generous support that immensely contributed to the success of this work. In addition, I am greatly indebted to staff working at animal nutrition laboratory and Parasitology laboratories, Department of animal Science and veterinary Parasitology, University of Jimma, for ever being available for me whenever I needed them during laboratory sample analysis. I extend appreciation to the field staff of the Ministry of Livestock Development in Jimma Zone for their cooperation during the entire study period. Special thanks go to our contact farmers in the district for their cooperation and availability whenever needed. Finally, I am grateful to my family for their moral support, understanding and prayers during the study period. vi Table of Contents Contents Page APPROVAL SHEET .................................................................................... Error! Bookmark not defined. DEDICATION............................................................................................... Error! Bookmark not defined. STATEMENT OF AUTHOR ................................................................................................................... iii BIOGRAPHICAL SKETCH .................................................................................................................... iv ACKNOWLEDGMENT ............................................................................................................................ v TABLE OF CONTENTS .......................................................................................................................... vi LIST OF TABLES ................................................................................................................................... viii LIST OF FIGURES ................................................................................................................................. ix LIST OF ABBREVIATION ....................................................................................................................... x ABSTRACT ................................................................................................................................................ xi 1. INTRODUCTION ................................................................................................................................... 1 1. 1 Null hypothesis (Ho) ........................................................................................................................ 4 1.3. Research question ............................................................................................................................ 4 2. LITERATURE REVIEW ...................................................................................................................... 5 2.1. Poultry Production Systems in Ethiopia ........................................................................................ 5 2.1.1. Large Scale Commercial Poultry Production System ................................................................. 5 2.1.2. Small Scale Intensive Poultry Production System ...................................................................... 5 2.1.3 Village or Backyard Poultry Production System ......................................................................... 5 2.1.3.1. Poultry Housing under Village Condition ............................................................................ 6 2.1.3.2. Poultry Feeding Under Village Condition ........................................................................... 7 2.1.3.3 Disease and Health Status under Village Poultry Production System .................................. 7 2.1.3.3.1. Infectious Diseases ............................................................................................................ 8 2.1.3.3.2. None Infectious Diseases ............................................................................................ 10 2.1.3.3.4. Poultry Health Management under Backyard Production System .............................. 12 2.2. Production Performance of the Ethiopian Poultry ..................................................................... 13 2.2.1. Production Performance of Indigenous Chickens ..................................................................... 13 2.2.2. Production Performance of Improved Chickens ....................................................................... 14 3. MATERIALS AND METHODS ......................................................................................................... 16 3.1. Description of Study Area ............................................................................................................. 16 3.2. Collection of Baseline Data for Problem Identification .............................................................. 17 3.2.1. Selection of Participating Households ...................................................................................... 17 3.2.2. Source of Data and Data Collection Instruments ...................................................................... 17 3.2.3. Identification and Prioritization of the Exotic Chicken Constraints ......................................... 18 3.2.4. Screening for Ectoparasites ....................................................................................................... 19 3.2.5. Faecal Collection and Analyses for Helminth Eggs ................................................................. 19 3.3. Basic Daily Supplementary Ration Formulation and Feeding Trial ......................................... 20 3.3.1. Preparation of Basic Supplementary Rations ............................................................................ 20 3.3.2. Management of the Experimental Chicken ............................................................................... 20 3.3.3. Measurement and Observation .................................................................................................. 21 3.3.3.1. Chemical Analysis of Feed Ingredients ............................................................................. 21 3.3.3.2. Production Performance ..................................................................................................... 22 3.3.3.3. Examining for Ectoparasites and Faecal Sample Collection for experimental chickens ... 22 3.3.3.4. Partial Budget Analysis ...................................................................................................... 22 3.4. Data Management and Analysis ................................................................................................... 23 4. RESULT ................................................................................................................................................ 24 4.1. Survey result ................................................................................................................................... 24 4.1.1. Demographic Background of the Respondents ......................................................................... 24 4.1.2. Constraints ranking ................................................................................................................... 25 vii 4.1.3. Seasonal changes and occurrence of exotic chicken death and diseases .................................. 28 4.1.4. Disease control .......................................................................................................................... 29 4.1.5. Management .............................................................................................................................. 31 4.1.5.1. Poultry housing system ...................................................................................................... 31 4.1.5.2. Feeds and feeding practices ............................................................................................... 31 4.1.5.3. Watering ............................................................................................................................. 32 4.1.6. Ectoparasites ............................................................................................................................. 33 4.1.6.1. Feed wise prevalence of ectoparasites ............................................................................... 34 4.1.7. Prevalence of gastrointestinal parasites .................................................................................... 35 4.2. Measurements and Observations during experiment ................................................................. 37 4.2.1. Chemical composition and nutritive values of rations .............................................................. 37 4.2.2. Feed Intake ................................................................................................................................ 37 4.2.3. Live weight change and animal performance ........................................................................... 38 4.2.4.Effect of dietary treatments on faecal egg counts ...................................................................... 40 4.2.5. Economics analysis ................................................................................................................... 41 5. DISCUSSION ........................................................................................................................................ 42 6. Conclusions and Recommendations .................................................................................................... 57 6.1 Conclusions ...................................................................................................................................... 57 6.2 Recommendations ........................................................................................................................... 59 REFERENCES .......................................................................................................................................... 60 viii List of Tables Tables Page Table 1: Purposively Selected Peasant Associations and randomly selected Participating Exotic Chicken Owners ......................................................................................................................................... 17 Table 2: Proportion of feed ingredient used in formulating experimental rations ..................................... 20 Table 3: Completely Randomized Experimental Design Used in this Study............................................. 21 Table 4: Demographic characteristics of the exotic chicken’s owner’s respondents in the study ............. 24 Table 5: Simple ranking constraints by exotic chicken farmers in the three PAs ...................................... 25 Table 6: Pair wise ranking on importance of exotic chicken constraints ................................................... 25 Table 7: Mortality and season of disease outbreak of chickens as reported by owners during ................. 29 Table 8: Summary of farmer’s responses on the various dimensions of handling chickens during .......... 30 Table 9: Housing management of exotic chickens reported in Kersa District during survey .................... 31 Table 10: Feeding management and season of feeding of exotic chickens reported in Kersa ................... 32 Table 11: Source and frequency of watering and watering equipment reported during survey ................. 32 Table 12: Types of ectoparasites fauna in free ranging chickens ............................................................... 33 Table 13: Species of ectoparasites and their sites of attachment ................................................................ 34 Table 14: Prevalence, risk factor, presence or absence of statistical significance difference in ................ 34 Table 15: Prevalence, risk factor, presence or absence of statistical significance difference in prevalence for each species of identified gastrointestinal parasites ............................................................. 36 Table 16: Chemical composition of treatment diets and feed ingredients used for experimental .............. 37 Table 17: Performance Characteristics of Layer Pullets Fed Experimental Diets ..................................... 38 Table 18: T-test-Comparing Initial body weight, Final body weight, Egg production and Dry matter intake by feeding group.............................................................................................................. 39 Table 19: ANOVA- Comparing Initial and Final body weight, Egg production and Dry matter intake by feeding group ............................................................................................................................ 39 Table 20: Economic Analysis of Egg Production ...................................................................................... 41 ix List of Figures Figures Page Figure 1: Map of the study area (ILRI, 2015) ............................................................................................. 16 Figure 2: Principal symptom observed by farmers ..................................................................................... 28 Figure 3: Effect of feed on faecal egg count ............................................................................................... 36 Figure 4: Effect of feed on faecal egg count ............................................................................................... 40 x LIST OF ABBREVIATION ANOVA Analysis of Variance AOA Association of Official Analytical Chemists BB Bovan Brown CRD Completely Randomized Design CSA Central statistical Agency DM Dry Matter DMRT Duncan's multiple range tests FAO Food and Agricultural Organization FGD Focus Group Discussion IB Isa Brown IBD Infectious Bursal Disease IBDV Infectious Bursal Disease Virus ILRI International Livestock Research Institute LIVES Livestock and Irrigation Value Chains for Ethiopian Smallholders ME Metabolizable Energy NCD Newcastle Disease NDV Newcastle Disease Virus NGO Non-Governmental Organizations OIE World Organization for Animal Health OR Odds Ratio PA Peasant Association PE Participatory Epidemiology PK Potchefstroom Koekoek RIR Rhode Island Red RRA Rapid Rural Appraisal SEM Standard Error of Mean SPSS Statistical Package for Social Sciences VVIBDV Very Virulent Strains of Infectious Bursal Disease Virus xi ABSTRACT The purpose of this study is to determine the constraints that limit exotic chicken productivity; assess and prioritize them determine the effect of supplementation on performance and parasitic burden and come up with appropriate measure that are sustainable in this livestock production system. The study was conducted in two phases in Kersa District of Jimma Zone. The first phase was a rapid rural appraisal study whereby identification and prioritization of exotic chicken constraints was carried out. Scarcity of feed and disease was found to be the most important constraint to exotic chicken’s production (about 71.7% of the total chicken deaths which is about 89.4% from scavenging only chickens). Green diarhoea and coughing, yellowish diarrhoea and sudden death were the most observed sign of disease in order of importance. Parasites were also found to be important diseases in the chickens. Other constraints identified and ranked were poor animal health service delivery, predator, poor housing, theft and inadequate poultry management skills among farmers in order of importance. The second phase was experimental study to evaluate the effect of feeding on dry matter (DM intake), egg production, mortality, parasitic burdon and profitability of Bovans brown layers. A total of 60 chickens with uniform body weight (BW) and age were randomly divided in to two groups each with 30 pullets. Finally, the two treatment rations were randomly assigned to each of the group of the experimental pullets in completely randomized design with three replicates for an experimental period of 3 months. Treatments were ration made from locally available ingredients and purchased commercial layer feed. All diets contained crude protein (CP) and metabolizable energy (ME) within the recommended level for layers. The CP and ME content of treatment rations were 16.45, 17.8% and 3233.48, 3366.60 kcal/kg DM, respectively. The DM intake of layers was 92.78 and 104.28g/bird/day (SEM = 0.70) for homemade and commercial feed respectively. Hen-day egg production (65.3 and 74.8% (SEM = 1.32)) and total egg produced per hen (58.8 and 67.3 (SEM =1.31) for homemade and commercial supplemented respectively) were better for commercial supplemented. Body weight change and egg mass was greater for commercial supplemented chickens. Helminth parasite prevalence was lower for commercial supplemented chickens (25%) than homemade supplemented (30%). The economic return (profitability) was higher in homemade supplemented chickens. In conclusion, the exotic chicken production suffers from the scarcity of feed, diseases, unavailability of reliable veterinary and extension services, inadequate knowledge and skills in the management of exotic chickens amongst the farming community. Supplementation of commercial feed significantly improved egg production, body weight gain and total profit but when feed cost is taken in consideration lower profit than homemade feed. It is recommended that extension packages that would enhance the knowledge and skills of the exotic chicken farmers on integrated interventions, be initiated and sustained for the improvement of the productivity of the birds. until concluded with further research, I recommend the producers to use mixed vitamin if homemade feed is fed. In general, supplementation of exotic chickens led to improvement in performance but cheaper feed ingredients are needed in order to get high economic gains. Key words: Poultry production constraints; Poultry feed; parasitic burden; feeding trial 1 1. INTRODUCTION Poultry production has an important economic, social and cultural benefit and plays a significant role in family nutrition in the developing countries. It occupied an important position in the provision of animal protein for human consumption (Yoriyo et al., 2008). The contribution of poultry to the global animal protein production is estimated to reach 40% in the year 2020, the major increase being expected in the developing world (Delgado et al., 1999). It has been estimated that 80% of the poultry population in Africa is found in the traditional backyard production system (Gueye, 2000), which makes substantial contributions to household food security (Muchadeyi et al., 2007), and Ethiopia is not exception to this situation. The poultry sub-sector of Ethiopia could be characterized into three major production systems based on some selected parameters such as breed, flock size, housing, feeding, health, technology and bio-security. These are village or backyard poultry production system, small-scale commercial poultry production system and large scale commercial poultry production system (Bush, 2006).The village or backyard poultry production system is constrained by many extrinsic factors among which high prevalence of disease, insufficient nutrition; poor management and inadequate bio- security are outstanding. Disease is considered to be the major threat to village poultry production. Infectious diseases, such as Newcastle disease, fowl cholera, fowl typhoid and fowl pox are common all over Ethiopia. In addition to infectious diseases, parasites and nutritional deficiency disorders are serious problems in the case of village chickens. Poor genetic potential due to lack of selection and losses to predation are also potential threats to the productivity of the village poultry (Whitmarsh, 1997). Different breeds of exotic chickens (Rhode Island Red, Australorp, New Hampshire and White Leghorns) were imported to Ethiopia since the 1950’s to be used either by their own or for upgrading of the local flock through crossbreeding. Since then higher learning institutions, research organizations, the Ministry of Agriculture and Non-Governmental Organizations (NGO’s) have disseminated many exotic breeds of chicken to rural farmers and urban-based small- scale poultry producers (Solomon, 2008). In the near past, there has been a substantial effort to introduce improved layer type chickens particularly Isa Brown (IB), Bovan Brown (BB) and dual- purpose hybrid Potchefstroom Koekoek (PK) to smallholder farmers to be kept under backyard 2 management system, particularly in Kersa district of Jimma Zone. At present, it is believed that the exotic breeds of chickens comprise about 2.2% of the total Ethiopian poultry population (CSA, 2012/13). Lack of recorded data on the production performance of exotic breeds of chicken makes it difficult to quantify the contributions of exotic breeds of chickens kept under rural objective condition (ILRI, 2015). Few of the available information tends to indicate that, most of the exotic breeds studied under village production system are not high yielding as the hybrids type used in the international poultry industry (FAO, 2010). Some of the bottlenecks of the introduced exotic breeds of chickens under farmer management condition include poor feeding and extension, poor veterinary services, lack of water, high prevalence of disease and predators, high mortality and lack of understanding of the complex biological, cultural and socioeconomic relationships involved in the production processes Moges et al., (2010), Getu and Birhan (2014) and Mengesha et al., (2011). Availability of commercial poultry ration is limited in and in the vicinity of Addis Ababa and is very expensive, especially for farmers in remote rural areas. The productivity of exotic breeds of chicken could be increased through improved feeding, the use of better adaptable breeds and adoption of better management and disease control system. On the contrary, it is believed that the local chickens are disease resistance and adaptable to the objective local conditions. However, it is difficult to improve the nutritional state of village poultry of local chickens, since the daily nutrient intake from scavenging is not exactly known (Smith, 2001), indicating that data on the nutritional status and supplementation requirement of chickens are lacking. Some of the available data tends to indicate that energy is critical during the rainy season in Ethiopia (Dessie, 1996), whereas protein supplementation, particularly that of essential amino acids are considered to be necessary for scavenging chickens during dry period (Rashid, 2003). One possible means of tackling such a problem is to study into the existing nutritional status of village or backyard poultry production system of the district and formulate daily basic supplementary ration based on the result of the assessment with the use of locally available feed ingredients. This being the cases, the specific objectives of this research proposal are 3 • To collect baseline data on productivity and general constraints of village or backyard poultry of exotic chickens in Kersa District of Jimma Zone. • Collect, identify and determine the chemical composition of the locally available feed ingredients found in Kersa District of Jimma Zone including that of the commercial ration available on market • To evaluate and develop daily basic supplementary ration based on the result of the laboratory chemical analytical data • To study the effect of supplementary ration developed on productivity and the parasitic durden on the exotic layers • To determine the comparative economic benefits of the basic daily ration developed as compared to commercial poultry ration available on the market 4 1. 1 Null hypothesis (Ho) Identification of production constraints and putting in place appropriate measures will not improve productivity of the exotic chickens. 1.3. Research question • What are external factor that influence the health and production performance of exotic chickens in Kersa District? • What are internal factor of owner’s perception affecting the health and production performance of exotic chickens in Kersa District? • How are the health and production performance of exotic chicken’s production in Kersa District improved? 5 2. LITERATURE REVIEW 2.1. Poultry Production Systems in Ethiopia The poultry sector in Ethiopia can be characterized into three major production systems based on some selected parameters such as breed, flock size, housing, feeding, health, technology and bio- security. These are large scale commercial poultry production system, small-scale commercial poultry production system and village or backyard poultry production system (Bush, 2006). 2.1.1. Large Scale Commercial Poultry Production System The large-scale commercial production system is highly intensive production system and involves an average of greater or equal to 10,000 birds kept under indoor conditions with a medium to high level of bio-security. This system depends on imported exotic breeds that require intensive inputs such as feed, housing, health, and modern management systems. It is estimated that this sector accounts for nearly 2% of the national poultry population. This system is characterized by higher level of productivity where poultry production is entirely market oriented to meet the large poultry demand in major cities. The existence of somehow better biosecurity practices has reduced chick mortality rates to about 5% (Bush, 2006). 2.1.2. Small Scale Intensive Poultry Production System Small-scale intensive production system is characterized by medium level feed, water and veterinary service inputs and minimal to low bio-security. Most of the small-scale poultry farms obtain their feed and foundation stock from large-scale commercial farms (Nzietchueng, 2008). There are few studies about diseases affecting poultry in this production system. Kinung’hi et al., 2004) mentioned coccidiosis as a cause of mortality, reduced weight gain and egg production and market value of affected birds. 2.1.3 Village or Backyard Poultry Production System In Ethiopia, indigenous chickens are the most widespread and almost every rural family owns chickens, which provide a valuable source of family protein and income (Tadelle et al., 2003). The country has diverse agro-climatic conditions favoring production of many different kinds of crops, 6 providing a wide range of ingredients and alternative feedstuffs suitable for poultry feeding. Making use of these resources to complement the scavenging resource base promises a considerable potential for success (Dessie and Ogle, 2001). Unfortunately, however village or backyard poultry production system is largely dependent on local chickens with little or no inputs. It is characterized by poor health care with minimal level of bio-security, high off take rates and high level of mortality. The system does not involve investment beyond the cost of the foundation stock and handfuls of local grains. Mostly, indigenous chickens are kept although some hybrids and exotic breeds may be kept under this system (Dawit et al., 2008). 2.1.3.1. Poultry Housing under Village Condition In traditional free range, there is no separate poultry house and the chickens live in family dwelling together with human beings (Solomon, 2007). The bio-security of the backyard poultry production system is very poor, as scavenging birds live together with people and other species of livestock. Poultry movement and droppings are very difficult to control and chickens freely roam in the household compound. There is no practice (or even viable means) of isolating sick birds from the household flocks and dead birds are left for either domestic or wild predators. Chickens and eggs are sold on open markets along with other food items. The current live bird marketing system represents a significant and potential hazard to both buyers and sellers, yet implementation of biosecurity and hygienic practices in such a system is generally difficult. The Newcastle Disease experience and the attitude of communities to handling sick birds (which are often sold) shows that marketing systems play a considerable role in the dissemination of disease over wide geographical areas in a relatively short period of time (Gebreab, 1995). Housing systems in the backyard poultry production system is rudimentary and mostly built with locally available materials if there is any. In summary, it is very difficult to apply health and bio-security measures on full day scavenging birds in small flock. Oges et al., (2010) reported that in Bure district, North West Ethiopia, 77.9% of the village chicken owners provide only night shelter and only 22.1% provided separate poultry house. Another study by Mengesha et al., (2011) in Jamma district and South Wollo reported that 41.3% and 21.2% of chicken owners share the same room and provided separate poultry house, respectively. 7 2.1.3.2. Poultry Feeding Under Village Condition Family chicken production is an appropriate system that makes the best use of locally available feed resources (Tadelle et al., 2003). Village chicken also play a role of converting household leftovers, wastes and insects into valuable and high-quality protein (Doviet, 2005). There is no purposeful feeding of chickens and scavenging is almost the only source of diet. Different feeding materials are present for scavenging including seeds, plant materials, worms, insects and unidentified materials (Tadelle and Ogle, 2000). Feed supplementation has been reported in various countries as a common practice to promote chicken performance. In Ethiopia, 99%, 97.5% and 98% of feed supplementation by chicken owners were reported by Halima (2007); Moges et al., (2010) and Mengesha et al., (2011), respectively. To make full use of the productive potential of hybrid layers adequate feed of high quality has to be provided. Ali (2002) reported that at least 60g/h/day of feed supplementation are needed for the scavenging cross birds. Scavenging laying hen could possibly obtain approximately 60 to 70% of their feed requirement from scavenging (Rahman et al., 1997). It is also reported that free-range scavenging chickens fulfill their nutrient requirements for protein, vitamins, and minerals from scavenging feed resources (Payne and Wilson, 1999; Dessie and Ogle, 2001), depending on factors such as available scavenging area per bird, quality of scavenging feed resources; season and production stage (Abdelqader et al., 2007). Maize is always the most preferred feed ingredient under every form of poultry production system (Benvenuti et al., 2012). Wilson (2010) suggested that provision of shelter, regular supplies of clean drinking water and some supplementary feeding would improve growth and reproductive rates and greatly increase survival at village level. 2.1.3.3 Disease and Health Status under Village Poultry Production System Under village poultry production system, prevalence of diseases, predators, lack of proper health care, poor feeding and poor marketing information were reported to the major constraint of poultry production (Moges et al., 2010; Dinka et al., 2010 and Mengesha et al., 2001). The high mortality of chicks under village chicken production system in the central highlands of Ethiopia is reported to be attributed to diseases, parasites, predation, lack of feed, poor housing and insufficient water supply (Tadelle, 2001). Among the infectious diseases, Newcastle disease, Salmonellosis, 8 Coccidiosis, Fowl pox and predators are considered to be the most important causes of mortality in local chicken (Eshetu et al., 2001). Disease Condition is any condition that interferes with the normal functioning of the cells, tissues, organs and systems of diseased animal. In poultry, disease condition could be caused by many factors including disease causing organisms, nutritional deficiencies, consumption of toxic substances, physical damage and internal and external parasitic infestations (CIRAD, 2005). Disease condition resulting from nutrient deficiencies, consumption of toxic substances and physical damage are referred to as non-infectious diseases. These diseases cannot be passed from bird to bird. Infectious diseases are caused by microorganisms such as parasites, fungi, protozoa, bacteria, mycoplasmas, chlamydia and viruses. These diseases are often also called contagious diseases to indicate that they can be passed from one bird to another either directly or indirectly (Dereje, 2002). 2.1.3.3.1. Infectious Diseases Newcastle disease is an infection of domestic poultry caused by virulent Newcastle disease virus (NDV). It is an acute respiratory disease, but depression, nervous manifestations, or diarrhea may be the predominant clinical form. Severity depends on the virulence of the infecting virus and host susceptibility. Occurrence of the disease may result in trade restrictions (petter and muller 2014). Newcastle disease viruses occur in three patho types: lentogenic, mesogenic, and velogenic, reflecting increasing levels of virulence (OIDE, 2000). The most virulent (velogenic) isolates are further subdivided into neurotropic and viscerotropic (Alexander, 1997). The disease can be present in healthy-appearing in cases of carrier exotic pets and birds and in a persistent carrier state, which has been demonstrated in the psittacine order (Erickson et al., 1977). Observations made by Nasser revealed that the velogenic strains of NCD virus are widely distributed throughout Ethiopia (Nasser, 1998). Transmission of Newcastle disease is via aerosols, birds, fomites, visitors and imported psittacines (often asymptomatic). It is not usually vertical (but chicks may become infected in hatcheries from contaminated shells). The virus survives for long periods at ambient temperature, especially in faeces and can persist in houses (in faeces, dust etc.) for up to 12 months. However, it is quite sensitive to disinfectants, fumigants and sunlight. It is inactivated by 9 temperatures of 56°C for 3 hours or 60°C for 30 min, acid pH, formalin and phenol, and is ether sensitive (Hadipour2009). Infectious bursal disease (also known as IBD, Gumboro Disease, Infectious Bursitis and Infectious Avian Nephrosis) is a highly contagious disease of young chickens caused by infectious bursal disease virus (IBDV). The disease is characterized by immuno suppression and high mortality generally at 3 to 6 weeks of age. The disease was first discovered in Gumboro, Delaware in 1962. It is economically important to the poultry industry worldwide due to increased susceptibility to other diseases and negative interference with effective vaccination. In recent years, very virulent strains of IBDV (vvIBDV), causing severe mortality in chicken, have emerged in Europe, Latin America, South-East Asia, Africa and the Middle East. Infection is via the oro-fecal route, with affected bird excreting high levels of the virus for approximately 2 weeks after infection (Caston et al., 2008). Salmonella are Gram negative, short plump shaped rods, non-spore forming and non- capsulated, aerobic and facultative anaerobic organisms and classified under the family Enterobacteriaceae (OIE Manual, 2006). More than 2300 serotypes of Salmonella have been identified, only about10% of these has been isolated from poultry (Gast, 1997). Chickens are the natural hosts for both S. Pullorum and S. Gallinarum (Snoeyenbos, 1991). Pullorum disease is usually confined to the first 2-3 weeks of age and occasionally occurs in adults (Shivaprashad, 1997). Fowl typhoid is frequently referred to as a disease of adult birds and there are also reports of high mortality in young chicks (Christensen et al., 1992). The epidemiology of fowl typhoid and pullorum disease in poultry, particularly with regard to transmission from one generation to the next are known to be closely associated with infected eggs (Wigley et al., 2001). Contaminated eggs produced by infected laying hens are thought to be one of the main sources of human infection with Salmonella Enteritidis (Humphrey et al., 1989). Eggs may become contaminated with Salmonella in two main ways: (i) Salmonella may silently infect the ovaries of apparently healthy hens and contaminate the eggs before the shells are formed. (ii) Salmonella infected bird droppings contain Salmonella that can contaminate the outer egg shells and may penetrate when crack the shell (Deryck and Pattron, 2004). 10 Coccidiosis is caused by various species of Eimeria, an Apicomplexa protozoan parasite. It is one of the common diseases in poultry, which is responsible for major economic losses worldwide (Razmi and Kalideri, 2000). The disease occurs only after ingestion of sporulated oocysts in susceptible hosts. Both clinically infected and recovered birds shed oocysts in their droppings, which contaminate feed, dust, water, litter, and soil. Oocysts may be transmitted by mechanical carriers (e.g., equipment, clothing, insects, farm workers, and other animals) (Hadipour et al., 2011). Coccidiosis occurs in the epithelial cells of the intestine, despite the advances in nutrition, chemotherapy, management, and genetics. E. tenella and E. necatrix are the most pathogenic and cause bloody lesions, high morbidity, and mortality (Gyorke et al., 2013). Most Eimeria spp. affect birds between 3 and 18 weeks of age and can cause high mortality in young chicks. Mixed infections are commonly found under field conditions. Coccidiosis in poultry is characterized by dysentery, enteritis, emaciation, drooping wings, and poor growth. Feed and water consumption are depressed. Weight loss, development of culls, decreased egg production, and increased morbidity and mortality may accompany outbreaks (Sharma et al., 2013). Bad management, such as wet litter that encourages oocyst sporulation, contaminated drinkers and feeders, bad ventilation, and high stocking density can exacerbate the clinical signs (Al-Natour et al., 2002). Knowledge regarding the farm conditions is necessary for developing the best prevention program, enabling the recognition of factors that influence the possibility of incidence of the disease (Shirzad et al., 2011) 2.1.3.3.2. None Infectious Diseases Disease condition resulting from nutrient deficiencies, predation, consumption of toxic substances and physical damage are referred to as non-infectious diseases. Nutritional deficiencies are widespread in Ethiopia and common in poultry production. For maximum performance and good health, poultry need a steady supply of energy, protein, essential amino acids, minerals, vitamins and adequate clean water. Recent advances in poultry nutrition have focused on three main areas: developing an understanding of nutrient metabolism and nutrient requirements; determining the availability of nutrients in feed ingredients; and formulating least-cost diets that bring nutrient requirements and nutrient supply together (FAO, 2015). 11 Practical poultry diets are formulated from a mixture of feed ingredients, including cereal grains, cereal by-products, fats, plant protein sources, animal by-products, vitamin and mineral supplements, crystalline amino acids and feed additives. In developing countries, the increasing cost and decreasing supply of traditional feedstuffs are expected to constrain the future expansion of poultry production. This situation highlights the urgent need to improve utilization of the wide range of locally available alternative feedstuffs. In many circumstances, feed resources are either unused and wasted, or used inefficiently. The use of most alternative feedstuffs is currently negligible, owing to constraints imposed by nutritional, technical and socio-economic factors. However, unlike intensive commercial poultry production systems, family poultry units and semi- commercial systems are well-suited to the inclusion of these feedstuffs (FAO, 2015). On the top of shortage and high cost of feed ingredients, one of the major nutritional problems in developing countries is the biological and chemical contamination of poultry feeds, which may have serious consequences on bird performance and the safety of poultry products in human nutrition. Of the potential contaminants, mycotoxins are the most widespread, particularly in hot, humid conditions (FAO, 2015). Clinical manifestation of nutrient deficiencies often occurs in conjunction with an alteration of normal biological processes that are unique for the nutrient. Some enzymes depend on particular vitamins and minerals for their functioning, and their activity diminishes when there are deficiency conditions. In other instances, a particular physiological response or change in metabolite concentration may occur. This information was primarily obtained from formal experiments in which the inadequacies were definitive. Under field conditions, nutrient inadequacies are usually marginal, occasionally multiple, and often confounded with management problems or disease (Lawrence et al., 2004). Predation is not common in commercial poultry production. However, predation is a big concern for backyard poultry producers. The reason for this difference is in the way flocks are housed and managed (Aaron and Ison, 2004). Commercial poultry producers maintain flocks within buildings during the entire production cycle. In the commercial sector, poultry houses and buildings are usually constructed with concrete foundations, complete roof, and enclosed fenced run areas. Commercial flocks are at risk from small predators and birds of prey when the building structures are not maintained (Sara and Spiegle, 2004). Backyard flocks, maintained by small farmers are usually housed in the existing barns that may not be adequate for keeping predators out. In some 12 cases, they may not be housed at all, allowing the birds to free-range and take cover under existing structures. Backyard poultry are also prone to predation if birds are raised on free-range, where they are allowed to scavenge freely. Flocks are at the highest risk, especially during the night, if they are not provided with any enclosed structure for protection (Teresa, 2004). 2.1.3.3.4. Poultry Health Management under Backyard Production System Many factors influence the health of smallholder chicken population which makes it even more difficult to design improvement strategies to overcome health constraints (Mapiye et al., 2008). High mortality is considered to be the major constraint to village chicken production systems (Muchadeyi et al., 2007). The effective control of diseases is an essential first step towards improving village poultry production (Ahlers et al., 2009). Moges et al., 2010) suggested that improvement in veterinary and advisory service could help to achieve control of diseases at village level. The same author reported 96.4% of village chicken owners had no culture of vaccination against poultry diseases in North West Ethiopia. Effective health coverage and vaccination programmes improved rural chicken performance in Pakistan (Javed et al., 2003). In village production study in different parts of Ethiopia, no vaccination practice against poultry diseases was reported to exist (Moges et al., (2010); Leta and Endalew (2010); Takele and Oli (2011). The Ethiopian National Veterinary Institute (NVI) produces a range of ND vaccines and provides them on request to modern poultry subsector. There is no national ND control policy or coordinated prevention and control program in rural Ethiopia. Vaccination against ND occurs in rural areas only in response to an outbreak. According to CACC (2003), the estimated number of vaccinated and treated animals in the country in the agricultural year of 2003 was estimated at 11.5 and 2.8 million of which 1.39% and 10% was poultry respectively. On the other hand the great majority of afflicted (56%) and dead (67%) animals were poultry. At the beginning of the agricultural year of 2003, the total chicken population was estimated at 42 million of which 0.37, 0.68, 26.4 and 24.2% was vaccinated, treated, afflicted and died respectively. The number of poultry died over the year was estimated at 10.2 million (24% of the national poultry population) resulting in the poultry population of 32 million at the beginning of 2004. 13 2.2. Production Performance of the Ethiopian Poultry 2.2.1. Production Performance of Indigenous Chickens In Ethiopia village poultry is rarely the sole means of livelihood for the household but is one of a number of integrated and complementary farming activities contributing to the overall well-being of the household. There is no reliable data indicating the annual contribution of village poultry for the national economic development in Ethiopia. Nevertheless, it is believed that rural poultry accounts for 99 percent of the national total production of poultry meat and eggs in Ethiopia (Tadelle et al., 2000). The most dominant chicken reared in Ethiopia are local ecotypes, which show a large variation in body conformation, plumage color, comb type and productivity (Halima et al., 2007). Generally, Tadelle et al. (2003) and Halima (2007) reported that the names of the indigenous chicken groups were being called as chicken-ecotypes and native-chickens, respectively. The egg production potential of local chicken is 30-60 eggs/year/hen with an average weight of 38g, under village management conditions, as compared to exotic breeds’ annual egg production of 250 eggs with mean egg weight of 60g (Alganesh et al., 2003) under intensive production in Ethiopia. According to (Alganesh et al., 2003) and (Negussie et al., 2003), the low productivity of the local scavenging hens is not only because they are low producers of small sized eggs and slow growers but also attributed to high chick mortality before they reach around 8 weeks of age. Moreover, the local chickens are the results of uncontrolled breeding between various local chicken ecotypes, which have not been selected by systematic breeding methods. The low production performance of the Ethiopian indigenous chickens’ os also attributed to the long natural reproductive cycle. It is by natural incubation and brooding that chicks are hatched and raised all over rural Ethiopia. A broody hen hatching, rearing and protecting few number of chicks (6-8) ceases egg laying during the entire incubation and brooding periods of 81 days. Yet the successes of the hatching and brooding process depend on the maternal instinct of the broody hen and prevalence of predators in the area, such as birds of prey, pets and some wild animals, all of which are listed as the major causes of premature death of chicks (Solomon, 2007). 14 2.2.2. Production Performance of Improved Chickens Production performance of exotic birds in Ethiopian condition needs to be monitored regularly to provide guidelines for policy makers. Lack of recorded data on the productive performance of chicken makes it difficult to assess the importance and contributions of the past attempts to improve the sector (Moges et al., 2010). All the available evidence indicates that all the imported breeds of chickens performed well under the intensive management system (Yami and Desie, 1997). In Ethiopia, the idea of distributing exotic chickens particularly Rhode Island Red (RIR) was to improve the productivity of local birds by mating them with improved cocks. According to Permin (2008), this scheme usually failed to work due to the fact that the introduced breeds could not adapt to the hot climate, low feeding and extensive management. The egg production potential of local chicken is 30-60 eggs year–1 hen–1 with an average of 38 g egg weight under village management conditions, while exotic breeds produce around 250 eggs year–1 hen–1 with around 60 g egg weight (Alganesh et al., 2003) in Ethiopia. With this potential of indigenous chicken, the demand of egg and chicken meat of Ethiopian populations cannot be satisfied (Geleta et al., 2013). The maximum number of eggs/year under Oromia Agricultural Research Institute for Fayoumi chicken (156 egg) was lower than 185 eggs year–1 hen–1 for Rhode Island Red and White Leghorn (176 eggs) but higher than Fayoumi (144 egg) as reported by Abraham and Yayneshet (2010) in North Ethiopia. Moreover, Alem (2014) reported average egg production per year per hen of exotic chicken (RIR) was 118.6 and 148.2 in lowland and highland agroecological zone of central Tigray, respectively. Most results showed that the overall performance of the crosses was better than either the native or the exotic parents under the existing management condition (Hailemariam, 1998; Aberra et al., 2005). From the report of CSA (2011), the average length egg-laying period/hen was also determined in breeds and environmental managements systems of which estimated numbers of days were 21, 36 and 105 days for local, hybrid and exotic breeds, respectively. Similarly, Alem (2014) reported average egg production per clutch per hen of exotic chicken (RIR) was 38.5 and 45.2 in lowland and highland agroecological zone of central Tigray, respectively. Sexual maturity of White Leghorn under intensive and extensive management ranged from 149-169 days (Demeke, 2004, 15 2007). Geleta et al. (2013) indicated that egg weight of Fayoumi chicken under Adami Tulu Research center (44.3 g) was similar to Fayoumi (43 g) but lower than egg weight for Rhode Island Red (52.5 g) and White Leghorn (52.1 g) reported by Abraham and Yayneshet (2010) in North Ethiopia. From this we can conclude that exotic breed and cross breed chicken can produce large number of eggs in the presence of adequate amount of feed. Poultry production is affected by factors such as breed and strain of chicken used, environmental conditions in poultry house, management practices and feed and feeding management (Bell and Weaver, 2002). The knowledge of performance of economic traits in chicken is important for the formulation of breeding plans for further improvement in production traits. Growth and production traits of a bird indicate its genetic constitution and adaptation with respect to the specific environment (Ahmed and Singh, 2007). The laying cycle of a chicken flock usually covers a span of about 12 months. Egg production begins when the birds reach about 18-22 weeks of age, depending on the breed and season. Flock production rises sharply and reaches a peak of about 90%, 6-8 weeks later, production then gradually declines to about 65% after 12 months of lay. There are many factors that can adversely affect egg production. Unraveling the cause of a sudden drop in egg production requires a thorough investigation into the history of the flock. Egg production can be affected by feed consumption (quality and quantity), water intake, intensity and duration of light received, parasite infestation, diseases, management and environmental factors (Jacob et al., 1998 and Fesha et al., (2010). 16 3. MATERIALS AND METHODS 3.1. Description of Study Area This study was conducted in Bulbul, Gello and Kitimbile Kebeles of Kersa district of Jimma Zone. Kersa district is located at 320 km South West of Addis Ababa. The altitude of the district ranges between 1740 and 2660 meters above sea level. About 58.6% of the total land of the district is arable of which 37.5% is under annual crops, 17.3% is pasture, 6.0% is forest, and the remaining 18.9% is considered swampy, degraded or otherwise unusable. The livestock resource of the study district comprises of 184,551 cattle, 12,364 sheep, 7,032 goats, 3,138 horses, 2,440 mules, 112 donkeys, 79,582 poultry, and 12,770 bee colonies. The total human population of the district is 165,391, of which 50% is reported to be male. Figure 1: Map of the study area (ILRI, 2015) 17 3.2. Collection of Baseline Data for Problem Identification 3.2.1. Selection of Participating Households Kersa district (Woreda) of Jimma Zone was purposively selected on the basis of poultry population, accessibility and the recommendation of Livestock and Irrigation Value Chains for Ethiopian Smallholders (LIVES) project. The three Kebeles (Peasant Association), namely Bulbul, Kitimbile and Gello were also purposively selected based on the accessibility and availability of exotic chicken either supplemented commercial feed or left as scavenging only. From the total of 360 exotic chicken owners, 180 owners were selected by using Krejcie & Morgan (1970) table for determining sample size of a known population. Then the 50% of the sample from each village was chosen to collect baseline data on productivity and general nutritional and health status of village or backyard poultry of exotic chickens in the district. Table 1: Purposively Selected Peasant Associations and randomly selected Participating Exotic Chicken Owners Peasant Association Total Exotic chicken owners Sampling fraction Participating owners Bulbul 176 0.5 88 Kitimbile 90 0.5 45 Gello 94 0.5 47 Total 360 180 3.2.2. Source of Data and Data Collection Instruments The primary data were collected with the use of pretested and structured questioners and focus group discussion (FGD). The primary data collected included: socio-demography of chicken keepers, ownership, and type of housing, feeds and feeding practices, diseases and perceived flock mortalities and animal health service provision. On site observations on other aspects of production such as housing, feeding, feed and water availability and ectoparasite and endoparasite prevalence were made and data were recorded. Focus group discussions were held with 24 farmers starting with the introduction of the investigator and explanation of the purpose of the discussion. Each focus group comprised of 6-9 participants and included a both female and male and the discussions were held in Farmer Training Centers in each village. The discussions were conducted in Afan Oromo language, on the overall potential and challenges of exotic chickens with the use of 18 checklist prepared in advance. Additional information’s on secondary data were obtained from Livestock Development and Extension Office. 3.2.3. Identification and Prioritization of the Exotic Chicken Constraints A Rapid Rural Appraisal (RRA) study with the use of Participatory Epidemiological (PE) tools was conducted to identify and prioritize the exotic chicken productivity constraints and capture the farmers’ perception of the exotic chicken production. To understand the effect of supplementation the survey includes two categories of exotic chicken’s owners keeping on scavenging with or without supplementary commercial ration. In the process of identification and prioritization, the specific item(s) identified by the respondents were written on cards. The cards were used for simple ranking of the item(s) in the desired order of importance (ILRI 2009), or for pair-wise ranking to compare individual with all the other items one-by-one to understand the relative importance of different constraint. The level of agreement between informants was assessed using Kendall’s coefficient of concordance. The participants were asked to identify poultry diseases found in the study area and the listed diseases were compared in terms of prevalence and mortality, using proportional pilling (Catley et al., 2011). All the data (information) collected on exotic chicken production and the prevailing poultry disease rankings were tabulated and weighted by awarding scores from 1-6 and 1-3, respectively, to each respondent (Catley et al., 2012). The cumulative sum of all the responses was considered as the weighted score for the particular constraint. Thus, the constraint with largest score was considered to be the most important. In the process of disease diagnosis and ranking, the clinical sign reported by the respondents were used to give tentative diagnosis and the respondents were asked to rank the diseases in order of prevalence and mortality rate. This was followed by the construction of seasonal calendar based on the information of the participating households. 19 3.2.4. Screening for Ectoparasites Thrusfield, (1995) formula would be used for calculating the adequate sample size in prevalence of ectoparasite study: - 𝑛 = 𝑧2p(1 − 𝑞) 𝑧2 Where n is the sample size, Z is the statistic corresponding to level of confidence, P is expected prevalence (that can be obtained from same studies or a pilot study conducted by the researchers), and d is precision (corresponding to effect size). The expected prevalence was 80% and we would like to take enough samples to be 90% sure that our estimate is within 10% of the actual prevalence. So, 60 chickens were included in the sample. Screening for ectoparasites involved a thorough examination of the body of the birds including the head, cloacal, brachial, ventral, and femoral areas. Those with parasites were identified and recorded. Samples of the observed ectoparasites were removed with a thumb forceps or brush and transferred to a Petri dish containing 10% formal saline. The samples were cleared with lactophenol and fixed on a microscopic slide using a little quantity of polyvinyl alcohol and lactophenol solution before detailed morphological examination. The scrape was collected and preserved in 70% ethanol. The mites were isolated from scrape, cleaned and mounted directly with Hoyerûs medium (Hoyerûs medium method) (Krantz, 1970). The identification was done using a compound microscope Lapage (1962) and Soulsby (1982). 3.2.5. Faecal Collection and Analyses for Helminth Eggs After thorough examination of each bird for ectoparasites, pooled sample were collected from the same birds examined for ectoparasites. The faecal sample were put into sample bottles and identified appropriately. The samples were later processed in the laboratory using the salt floatation technique with saturated sodium chloride and sugar solution as the floating medium (Hansen 1990). Demonstration of the parasites was by microscopic examination of smears made after the concentration method. The identification keys of Soulsby (1982) and Khali et al., (1994) were adopted. 20 3.3. Basic Daily Supplementary Ration Formulation and Feeding Trial 3.3.1. Preparation of Basic Supplementary Rations The feed ingredients used for the formulation of experimental ration (homemade) used in this study were corn grain (CG), wheat (W), soybean meal (SBM), noug seed (NS), limestone, and salt. The CG, SBM, NS, and wheat were grounded before mixing and subjected to laboratory chemical analysis (DM, CP, EE, CF and ash) according to the proximate method of analysis (Table 2). The homemade, experimental ration was prepared to contain about 2800-2900 Kcal of metabolizable energy (ME) per Kg of dry matter (DM) and 16- 17% of crude protein (CP) to meet the energy and protein requirement of layers respectively. Table 2: Proportion of feed ingredient used in formulating experimental rations Experimental feed T1 Ingredients (%) CG 44 WS 29 SBM 14 NS 7.5 LS 5.0 Salt 0.5 Total 100 CG= corn grain; WS= wheat short; SBM= soybean meal; NS = noug seed; LS= limestone; T1 =homemade feed 3.3.2. Management of the Experimental Chicken Sixty (60), 3-month old pullets of Bovans Brown breed of chicken were purchased from Bishoftu Research Center. These were individually weighed, examined for general health status (Some points observed include behaviors (such as eating and drinking), attitude, gait, feathers (are they ruffled?) and personality of the bird in the group (fearful, aggressive, alert). The various body areas (eyes, nostrils, beak/oral cavity, ears, respiratory system, skeletal system, wings, feet, legs, weight/muscles) examined to determine if a problem exists. Feces also observed for their consistency) and divided in to two groups each with 30 pullets. Each of these groups was further sub-divided into 3 groups each with 10 pullets, making a total of 6 groups each with 10 pullets housed in an experimental cage. Prior to start of the trial the chickens were treated with broad- 21 spectrum anthelmintic (Levamisole 30% IP; Smith Kline Pharmaceuticals Limited, India, at 30 mg per kg body weight through drinking water). (Ox tetracycline 20% W.S.P.) Was chosen and administered as a broad-spectrum antibiotic. The dosage was at 0.5g per 1 liter of drinking water for 5 days. This was to ensure that no other bacterial disease would affect the chickens during the experiment. Finally, the two treatment rations shown in table 3 were randomly assigned to each of the group of the experimental pullets in completely randomized design with three replicates for an experimental period of 3 months. Table 3: Completely Randomized Experimental Design Used in this Study Dietary treatments Types of treatments Replication 1 2 3 Total 1 Homemade diet 10 10 10 30 2 commercial diet 10 10 10 30 Total 20 20 20 60 120g of supplementary feeds (homemade and commercial ration) were offered once per day and water is made available all the times. Body weight of the pullets was measured weekly and eggs were collected daily and recorded. 3.3.3. Measurement and Observation 3.3.3.1. Chemical Analysis of Feed Ingredients Representative samples (3g) from each were taken from each of the feed ingredients and subjected to laboratory chemical analysis before formulating the homemade treatment ration (T2). The results of the analysis were used to formulate the ration. The samples were analyzed for dry matter (DM), ether extract (EE), crude fiber (CF) and total ash according to AOAC (1990). Nitrogen (N) content was determined by Kjeldahl procedure and crude protein (CP) was calculated as Nx6.25. The total metabolizable energy content was estimated by using the formula of Wiseman (1987) as: ME (Kcal/kg DM) = 3951 + 54.4 EE – 88.7 CF – 40.8 Ash. Chemical analyses of feeds were done in Animal Nutrition Laboratories of JUCAVM. 22 3.3.3.2. Production Performance Mean daily feed intake, body weight change, feed conversion ratio and rate of egg production were used to measure the production performance of the experimental pullets. Mean feed consumption was determined as the difference between the feed offered and refused. The experimental birds were weighed individually using analytical balance. Average bird weight was calculated as sum of individual weight of birds divided by number of birds. Average body weight gain or loss for each replicate was calculated by subtracting the initial weight from the final weight. Feed conversion efficiency was estimated as a ratio of the weight of feed consumed per unite body gain and per egg weight produced. Eggs were collected per day and rate of lay was expressed as the average percentage hen-day egg production based on the average values from each replicate Hunton (1995). 3.3.3.3. Examining for Ectoparasites and Faecal Sample Collection for experimental chickens After thorough examination of each bird, for ectoparasites, fresh faecal samples were collected in sterile polythene bags. The collected samples were placed into an airtight cool box and brought to JUCAVM Parasitology Laboratory and were refrigerated at 4ºC until analysis for the investigation of the parasites. The samples were screened using saturated sodium chloride floatation techniques (Mc Nabb et al., 1985). 3.3.3.4. Partial Budget Analysis Partial budget analysis was done according to Upton (1979) to determine the comparative economic benefit of the treatment rations. Total variable cost includes cost of feeds, cost of transportation and cost health during the experiment for each treatment group. Total return (TR) was considered as difference in sale and purchase price. The net income (NI) was expressed by subtracting total variable cost (TVC) from total return (TR). • NI=TR-TVC The change in net income (ΔNI) was expressed as the difference between the change in total return (Δ TR) and total variable cost (Δ TVC). 23 • ΔNI = Δ TR - Δ TVC The marginal ret of return (MRR) measures the increase in net income (ΔNI) related to each additional unit of expenditure (Δ TVC) and expressed in percentage. MRR= ΔNI / Δ TVC 3.4. Data Management and Analysis Probing was used for description and clarification of the data at different stages of the process of information gathering and Kendall’s coefficient of concordance was used to indicate the level of agreement. All the data obtained from the field were properly organized and prepared for codification. The collected data were coded and entered in to Microsoft Excel spread sheet and analyzed using Statistical software for Social Sciences (SPSS) version 20. Frequency was used to calculate the prevalence. Chi-square was used to test the statistical significance difference between the risk factor groups in prevalence of parasite infestation. Odd ratio was used to estimate risk in groups of risk factors at 90% confidence interval (CI). P-value less than 0.05 (p<0.05) was considered as statistical significance difference. The data collected from experimental chickens were analyzed and ANOVA analysis was made to examine and understand relationship between the commercial and the homemade ration supplemented group and the observed score of Initial body weight, Final body weight, Egg production and the observed parasite score. 24 4. RESULT 4.1. Survey result 4.1.1. Demographic Background of the Respondents Table 4 revealed that the proportion of female respondents were higher than males in three PAs. The analysis for educational status disclosed that 88.5% in Bulbul, 63.8% in Gello and 62.2% of the respondents in Kitimbille were illiterates. Others can write and read and involved in formal education such as elementary school and high school. About 93.3% of the respondents were fully involved in mixed farming activities as means of livelihood. Most of the respondents (62.8%) were between the ages of 23-33 years in all PAs. Table 4: Demographic characteristics of the exotic chicken’s owner’s respondents in the study Frequency (N (%)) Variables Categories Bulbul Gello Kitimbille Total Gender male 18(20.5%) 16(34.0%) 8(17.8%) 42(23.3%) female 70(79.5%) 31(66.0%) 37(82.2%) 138(76.7%) Educational status illiterate 78(88.5%) 30(63.8%) 28(62.2%) 136(75.6%) literate 10(11.4%) 17(36.2%) 17(37.8%) 44(24.4%) Source of income mixed farm 82(93.2%) 44(93.6%) 42(93.3%) 168(93.3%) poultry production 4(4.5%) 2(4.3%) 0(0%) 6(3.3%) other 2(2.3%) 1(2.1%) 3(6.7%) 6(3.3%) Age 23-33 50(56.8%) 30(63.8%) 33(75.3%) 113(62.8%) 34-46 38(43.2) 17(36.2%) 12(26.7%) 67(37.2%) 25 4.1.2. Constraints ranking Table 5; presents lists of exotic chicken constraints ranked in order of importance in the three PAs. The ranking of constraints by the stakeholders was almost similar in the three study villages. Table 5: Simple ranking constraints by exotic chicken farmers in the three PAs Constraints Focus group discussions Bulbul Kitimbille Gell o R (sum of the rank) R-A D2∑(R-A) Scarcity of Feed 5 6 6 17 10.5 110.25 Disease 6 5 5 16 9.5 90.25 Poor animal health service 2 4 4 10 3.5 12.25 Predator 4 3 3 10 3.5 12.25 poor housing 1 1 2 4 -2.5 6.25 Theft - - 1 1 -5.5 30.25 Conflict - - 1 1 -5.5 30.25 Inadequate skills - - - - -6.5 42.25 - - - - -6.5 42.25 A=59/9=6.5 S= 376.25 ❖ W=12S/m2 (N) (N2-1) ➢ Where ✓ S= sum of the square of the R- from the A ✓ m= number of respondents ranking the constraints ✓ n= number of constraints that is evaluated by respondents ➢ From the data ✓ s= 376.25 ✓ m=3 ✓ n=9 W= 4515/9(9) (80) = 4515/6480 = 0.69 27 Table 6: Pair wise ranking on importance of exotic chicken constraints Principal symptoms observed by farmers, prior to the death of their chickens Disease Poor animal health service Conflict feed Low attitude Predator Theft Inadequate skills Poor housing Disease disease Disease feed disease disease disease disease disease Poor animal health service Poor animal health service feed Poor animal health service Poor animal health service Poor animal health service Poor animal health service Poor animal health service Conflict feed conflict predator conflict conflict conflict Feed scarcity feed feed feed feed feed Low attitude predator theft Inadequate skill Poor housing Predator predator predator predator Theft theft theft Inadequate skills Poor housing Poor housing 28 Figure 2: Principal symptom observed by farmers 4.1.3. Seasonal changes and occurrence of exotic chicken death and diseases Seasonal patterns of the diseases were similar in the three villages. Participants identified a number of seasonal risk factors, and believed that early morning dew (obsessrved throughout the wet season) was the most important risk factor for disease occurrence. Another seasonal factor included the early onset of the wet season (referred to as early rain or early grass growth). Alternatively, some farmers simply identified the wet season as increasing risk. Farmers confirmed that they experienced losses in their chickens as illustrated in table 12. Basically, losses were due to diseases, nutrition and parasites. The extent and severity of losses were reported to be seasonal (Table 12) with the greatest magnitude of losses (57.8%) occurring during the rainy season. 29 Table 7: Mortality and season of disease outbreak of chickens as reported by owners during villages Parameters Bulbul N =88 Gello N=47 Kitimbille N=45 Overall N=180 Mortality Dry Season 11(12.5%) 5(10.6%) 10(22.2%) 26(14.4%) Rainy Season 49(55.7%) 30(63.8%) 24(53.3%) 103(57.2%) Total 60(68.2%) 35(74.5%) 34 (75.5%) 129(71.7%) Season of Disease Outbreak Rainy Season 67(76.1%) 33(70.2%) 29(64.4%) 129(71.7%) Dry Season 15(17%) 11(23.4%) 10(22.2%) 36(20%) 4.1.4. Disease control Table 8 presents the proportions of indigenous chicken farmers who used various disease control methods and animal health service provision in Bulbul, Gello and Kitimbille villages. Small percentage of households used to isolate sick birds in Bulbul (3.4%) and Gello (2%) but none of households isolate sick birds in Kitimbille. According to the majority (94.4%) of the respondents, there is no control of the free movement of birds, except (11.4%) of households in Bulbul. All of the respondents reported to throw away dead birds. About 17.2% of the respondents vaccinate their chickens whereas about (82.8%) of respondents reported to have no history of vaccination. Animal health service delivery was poor in the three villages, with less than 10% of the farmers receiving services from Government in the three villages. About 6% of the farmers in the three villages used human antibiotics, particularly tetracycline capsules for treating their chickens. 30 Table 8: Summary of farmer’s responses on the various dimensions of handling chickens during survey Frequency (N (%)) Variables Categories Bulbul Gello Kitimbille Total Isolate sick bird Yes 3(3.4%) 1(2%) 0(0%) 4(2.2%) No 85(96.6%) 46(97.8%) 45(100%) 176(97.2%) Extension package Good 21(23.8%) 5(10.6%) 6(13.3%) 32(17.8%) Poor 67(76.1%) 42(89.4%) 39(86.7%) 148(82.2%) Control free movement Yes 10(11.4%) 0(0%) 0(0%) 10(5.6%) No 78(88.6%) 47(100%) 45(100%) 170(94.4%) Throwing dead bird on field Yes 88(100%) 47(100%) 45(100%) 180(100%) No 0(0%) 0(0%) 0(0%) 0(0%) Vaccination Yes 21(23.9%) 4(8.5%) 6(13.3%) 31(17.2%) No 67(76.1%) 4.3(91.5%) 39(86.7%) 149(82.8%) Access to vet. service Yes 11(12.5%) 2(4.3%) 2(4.4%) 15(8.3%) No 77(47.5%) 45(95.7%) 43(95.6%) 165(91.7%) Human drug Yes 5(5.7%) 3(6%) 2(4.4%) 10(5.6%) No 73(82.9%) 44(93.6%) 43(95.5%) 160(88.8%) 31 4.1.5. Management 4.1.5.1. Poultry housing system The results of poultry housing and facilities assessment are presented in Table 9. Table 9: Housing management of exotic chickens reported in Kersa District during survey villages Parameters Bulbul N=88 Gello N=47 Kitimbille N=45 Cumulative N=180 Housing Share main house 23(26.1%) 3(6.4%) 9(20%) 35(19.4%) Separated shelter 65(73.9%) 44(93.6%) 36(80%) 145(80.6%) Perch inside 17(73.9%) 0(0%) 8(88.9%) 25(73.5%) On ceilings of the house 6(26.1%) 3(100%) 1(11.1%) 10(28.6%) Constructed based on recommended package 9(10.2%) 7(14.9%) 5(11.1%) 22(12.2%) Frequency of cleaning Once per day 66(75%) 40(85%) 34(75.5%) 138(76.6%) Every two days 4(4.5%) 5 (10.6%) 7(15.6%) 16(8.5%) Every 3 to 6 days 0(0%) 2(4.2%) 1(2.2%) 3(1.7%) Per week 9(10.2%) 2(4.3%) 8(17.7%) 19(10.6%) Never 3(3.4%) 0(0%) 3(6.7%) 6(3.2%) 4.1.5.2. Feeds and feeding practices In all the PAs studied, 61.7% of the respondents reported to use only scavenging with no additional feed supplements for chicken except a few households (12.2%) supplemented kitchen left over and some grain very rarely. About 26.1% of the respondents were using purchased commercial feeds (Table 10). 32 Table 10: Feeding management and season of feeding of exotic chickens reported in Kersa villages Parameters Bulbul N=88 Gello N=47 Kitimbille N=45 overall N=180 Feed system purchased feed supplemented 16(18.2%) 10(21.3%) 11(24.4%) 47(26.1%) Scavenging only 62(70.5%) 32(68.1%) 27(60%) 111(61.7%) Kitchen left over and some grain 10(11.4%) 5(10.6%) 7(15.6%) 22(12.2%) Feed availability Harvesting season 54(61.4%) 32(68.1%) 38(84.5%) 124(68.9%) Wet season only 14(15.9%) 8(17%) 4(8.9%) 226(14.4%) Dry season 3(3.4%) 2(4.2%) 0 5(2.8%) Feeding troughs Regular feeding troughs 0 0 0 0 Flat plastic 12(13.6%) 7(14.8%) 5(11.1%) 24(13.3%) locally made wood 2(2.3%) 1(2.1%) 0 3(1.7%) any broken material 4(4.5%) 3(6.3%) 6(13.3%) 13(7.2%) 4.1.5.3. Watering Information recorded for frequency of watering (Table 11) revealed that about 63.9% of respondents provide water with free access in the three PAs. Only 11.1% of the respondents provide water in the afternoon only. A few respondents 19.4% were provide water both in morning and evening. Regarding source of water, river water was the major source (55.6%), whereas pond water accounts for about 31.1% of the total water supply. Borehole accounts for the rest of the proportion (13.3%) as water source. The majority of the respondents (57.7%) used any broken material as watering trough while 40.6% used plastic materials. But none of respondent used regular watering troughs.More than half (69.4%) of the respondents never cleaned watering trough, which indicates poor sanitation. Table 11: Source and frequency of watering and watering equipment reported during survey 33 villages Source and frequency of watering Bulbul N=88 Gello N=47 Kitimbille N=45 overall N=180 Frequency of watering Free access 48(54.5%) 32(68.1%) 35(77.8%) 115(63.9%) Afternoon only 12(13.6%) 5(10.6%) 3(6.7%) 20(11.1%) Morning and evening 18(20.5%) 10(21.3%) 7(15.6%) 35(19.4%) Water sources River water 33(37.5%) 28(59.6%) 39(86.7%) 100(55.6%) Borehole water 16(18.2%) 8(17%) 0(0%) 24(13.3%) Pond water 39(21.7%) 11(23.4%) 6(13.3%) 56(31.1%) watering troughs Regular watering troughs 0(0%) 0(0%) 0(0%) 0(0%) Plastic tray 35(39.7%) 17(36.2%) 21(46.7%) 73(40.6%) locally made wood 2(2.3%) 1(2.1%) 0(0%) 3(1.7%) any broken material 51(57.9%) 29(61.7%) 24(53.3%) 104(57.7%) Frequency of cleaning daily 6(6.8%) 0(0%) 0(0%) 6(3.3%) twice per week 9(10.2%) 2(4.3%) 0(0%) 11(6.1%) once per week 27(30.7%) 7(14.9%) 4(8.9%) 38(21.1%) never 46(52.3%) 38(80.6%) 41(91.1%) 125(69.4%) 4.1.6. Ectoparasites Two species of mites of the genus Acari, three species of lice of the genus Mallophaga, one species of tick of the genus Argasidae and one species of flees of the genus Echidnophaga were identified in the study areaduring survey (Table 12). Table 12: Types of ectoparasites observed during survey inthe study area Ectoparasites No. Examined No. Infested Prevalence (%) 95% CI Mites 60 30 50 1.08-9.95 Lice 60 44 73.3 0.32-32.9 Ticks 60 16 26.7 0-12.25 Fleas 60 20 33.3 0.41-6.47 The species of ectoparasites registered in present survey includes, M. gallinae which had the highest prevalence 46.7% followed by, C.mutans, Echidenophaga gallinacean, M. stramineus,, A. 34 persicus, D.gallinae and C. heterographus, with a respective prevalence of 40%, 33.3%, 30%, 26.7%, 23.3% and 16.7%, respectively. These ectoparasites have commonly been identified in free range chickens (Ekpo et al., 2010). Table 13: Species of ectoparasites and their sites of attachment Species of Ectoparasites No. infested Prevalence (%) 95%CI Sites of attachments Mites 30 50 Cnemidocoptes mutans 24 40 1.06-9.3 Lower limb (non-feather part) Dermanyssus gallinae 14 23.3 0.62-7.4 mixed 8 Lice 44 73.3 Cuclotogaster heterographus 10 16.7 0.41-6.5 Comb, head, neck Menopon gallinae 28 46.7 0.8-6.4 Thigh, wing, leg,chest, theshoulders and the back of birds Menacanthus stramineus 18 30 0.62-5.9 Vent,head, fluffy feather mixed 12 Ticks 25 47.1 Argas persicus 25 47.1 2.46-60.9 Ventral abdominal area and below wings Fleas 20 33.3 Echidnophaga gallinacean 20 33.3 1.5-16.56 Comb, wattles, eyes and around the ears 4.1.6.1. Feed wise prevalence of ectoparasites Out of the 60 chickens examined for ectoparasites 46 chickens were infested with an overall prevalence of 76.7%.In this study, higher infestation rate was observed in scavenging with no supplemented chickens 96.7% than scavenging with supplemented birds (56.7%) and the difference was statistically significant (p<0.05). Table 14: Prevalence, risk factor, presence or absence of statistical significance difference in 35 prevalence for each species of identified ectoparasites Species of Ectoparasites Scavenge only Scavenge plus supplement OR 95%CI X2 P-value Cnemidocoptes mutans 16(53.3%) 8(26.7%) 3.14 1.06-9.3 4.4 0.032 Dermanyssus gallinae 15(50%) 11(36.7%) 1.7 0.6-4.8 1.06 0.217 Cuclotogaster heterographus 5(16.7%) 4(13.3%) 1.3 0.3-5.4 0.13 0.5 Menopon gallinae 17(56.7%) 11(36.7%) 2.25 0.8-6.4 2.44 0.098 Menacanthus stramineus 11(36.7%) 7(23.3%) 1.9 0.6-5.8 1.27 0.19 Argas persicus 17(56%) 8(26.7%) 3.6 1.2-10.6 5.5 0.18 Echidnophaga gallinacean 15(50%) 5(16.7%) 5 1.5-16.5 7.5 0.006 Overall 29(96.7%) 17(56.7%) 22.17 2.66-184.7 13.4 0.000 As we can observe from the result of the above Table 15, scavenging with no supplemented group (OR=22.17 p<0.05), thus the result indicates that there is statistical significance relationship between the scavenging plus supplemented and scavenging without supplemented group on the prevalence of ectoparasites. The odds of ecto-parasitic prevalence for scavenging chickens with no supplementations are 22.2 times the odds of parasitic ecto-prevalence of scavenging with commercial feed supplemented There was a significant association between feeding and parasitic prevalence (X2(1)>=13.4, P<0.001). 4.1.7. Prevalence of gastrointestinal parasites From a total of 60 examined chickens 28 (62.2%) were found positive for gastrointestinal parasite eggs. About 53.3% and 26.7% of the chickens were positive for nematodes and cestodes species, respectively. The association of prevalence of gastrointestinal nematodes and cestodes species with the considered risk factors were shown in Table 15. There is statistically a significant difference in the overall prevalence of GIT helminth parasites between commercial supplemented and Scavenging only chickens (P <0.05). During the survey, the commonly recorded ova were of A. galli (26.7%), H. gallinarum (40%), Capillaria spp. (13.3%), Raillietina, spp, (26.7%). 36 Table 15: Prevalence, risk factor, presence or absence of statistical significance difference in prevalence for each species of identified gastrointestinal parasites Species of Ectoparasites Scavenge only Scavenge plus supplement OR 95%CI X2 P-value Capillaria species 5(16.7%) 3(10%) 1.8 0.39-8.3 0.57 0.353 Heterakis gallinarum 16(53%) 6(20%) 4.57 1.45-14.39 7.17 0.007 Ascaridia galli 12(40%) 5(16%) 3.3 0.99-11.12 4.02 0.042 Raillietina tetragona 7(23%) 6(20%) 1.22 0.35-4.17 0.098 0.500 Raillietina echinobothrida 2(6.7%) 0 - 0.848-1.03 2.069 0.246 Raillietina cesticillus 4(13%) 7(23%) 0.50 1.31-1.95 1.002 0.253 Total 23(76%) 14(46.7%) 3.75 1.24-11.34 5.71 0.016 The lower Mean faecal egg counts (FECs) were recorded in the scavenging with supplemented chickens as compared to the scavenging without supplementation (Figure 2). 0 100 200 300 400 500 600 700 800 900 E g g p er g ra m o f fa ec es figure 3: Faecal egg count in scavenging with and without commercial feed Scavenge only Commercial supp 37 4.2. Measurements and Observations during experiment 4.2.1. Chemical composition and nutritive values of rations The results of the chemical analysis of ingredients used and nutritional composition of the ration for the treatment are given in Tables 19. Table 16: Chemical composition of treatment diets and feed ingredients used for experimental Chemical composition (%) Ingredients DM CP EE CF Ash ME Kcal/kg CG 89.5 8.7 5.9 5.2 4.21 3638.95 WS 90.3 14.3 5.1 7.4 4.8 3376.22 SBM 93.2 38 7.8 6.1 6.4 3384.15 NS 92.5 28.5 9.2 17.2 8.2 2694.53 Treatments Homemade 89.175 16.45 6.2 7.2 10.2 3233.48 Commercial 91.56 17.8 4.32 6.8 5.3 3366.60 DM= dry matter; CP= crude protein; EE = ether extract; CF= crude fiber; CG=Corn grain; WS=Wheat short; SBM=Soybean meal; NS=Noug seed; ME=methabolizable energy 4.2.2. Feed Intake The effect of using locally available ingredients and commercial feed in layers ration on dry matter (DM) intake and performance of layers is shown in Table 18. Intake of DM was higher in commercial feed supplemented birds. 38 Table 17: Performance Characteristics of Layer Pullets Fed Experimental Diets Parameters % Dietary Treatments Homemade Commercial DMI (g/bird/day) 92.78 104.28 Initial body Weight (g)/bird) 962.5 967.75 Final Body Weight (g/bird) 1492.7 1553.25 Body Weight Gain (g/bird) 530.2 585.5 Total Feed Consumed (kg) 250 281 Eggs produced (per bird/wk) 4.9 5.61 Total Eggs produced 1764 2019 Hen Day production (%) 65.3 74.8 Mortality - - Egg mass (g/hen/d) 24.8 29.3 FCR 3.7 3.5 4.2.3. Live weight change and animal performance Initial BW (g) in experimental chickens did not differ significantly (P<0.05) among dietary treatments; however, final BW and total BW gain (g) for the period of 90 days were significantly (P<0.05) higher in commercial feed supplemented groups relative to homemade supplemented group. Feed conversion ratio (FCR) by chickens (g DMI g-1 gain) was also significantly (P<0.05) better in commercial supplemented groups as compared to homemade supplemented group. Egg production during the experimental period was significantly (P<0.05) better in commercial supplemented birds as compared to homemade supplemented birds. 39 Table 18: T-test-Comparing Initial body weight, Final body weight, Egg production and Dry matter intake by feeding group Group Statistics Group N Mean Std. Deviation Std. Error Mean Initial body weight Homemade 30 966.67 4.381 0.800 commercial 30 967.7 3.816 0.697 Final body weight Homemade 30 1444.37 9.478 1.730 commercial 30 1490.33 8.976 1.639 Egg production Homemade 30 58.13 4.04 0.74 commercial 30 67. 2 4.5 0.80 Dry matter intake Homemade 30 92.70 3.56 0.65 commercial 30 104.23 5.26 0.96 Table 19: ANOVA- Comparing Initial and Final body weight, Egg production and Dry matter intake by feeding group ANOVA Group Sum of Squares Df Mean Square F Sig. Initial body weight Between Groups 16.017 1 16.017 0.949 0.334 Within Groups 978.967 58 16.879 Total 994.983 59 Final body weight Between Groups 31694.017 1 31694.017 371.993 0.000 Within Groups 4941.633 58 85.201 Total 36636.65 59 Egg production Between Groups 1233.07 1 1233.07 67. 20 0.000 Within Groups 1064.27 58 18.35 Total 2297.33 59 Dry matter intake Between Groups 1995.27 1 1995.27 98.94 0.000 Within Groups 1169.66 58 20.16 Total 3164.90 59 40 4.2.4.Effect of dietary treatments on faecal egg counts From a total of 60 experimental chickens examined 14 (23.3%) were found positive for gastrointestinal parasite eggs prior to treatment with anthelminthic (Table 19). Prevalence and species of gastrointestinal parasite identified prior to treatment Species of parasites No.of chicken examined No.of positive chickens Nematodes Ascaridia galli 60 9(15%) Heterakis gallinarum 60 5(8%) Eimmiria species 60 7(11.6%) Cestodes Raillietina tetragona 60 4(6.7%) Total 60 14(23%) The mean faecal egg counts (FECs) (per gram) were slightly the same in both treatments when assigned to this different treatment and started decrease in both treatments. No appreciable difference in FECs was noticed up to 3rd period of experimentation in the two groups of chickens. A significant increment in the EPG was recorded in the homemade feed supplemented chickens after 3rd period whereas, in commercial feed supplemented groups the FECs remained unchanged throughout the 90 days experimental period (Figure 5). Figure 4: Effect of feed on faecal egg count 0 50 100 150 200 250 300 350 400 I II III IV V VI Fortnight F ae ca l eg g c o u n t (e g g p er g ra m ) Homemade Commercial 41 4.2.5. Economics analysis Table 26 shows the economics of raising exotic chickens under homemade and improved feed management. Higher gross margin, net profit and return per birr invested were obtained from homemade feed supplemented chickens, compared to commercial feed supplemented chickens. The MRR implied that each additional unit of 1 birr per hen egg cost increment resulted in 1 birr and additional 1.326 ETB profit for T1. Even though, layers in T2 showed higher percentage of egg production, it was not found economically feasible as compared to layers in T1. On the other hand, T1 was found to be profitable and economically feasible due to relatively lower cost. Assuming that capital is not a constraint, the technology with the highest change in net return is chosen. However, if cost of feed change, recommendation could be change based on the existing conditions for egg production. Table 20: Economic Analysis of Egg Production Parameters % Dietary Treatments T1 T2 Total Feed Consumed (g/bird) 250 281 Cost of total Feed Consumed/treatment 2275 3186.5 Feed Cost per Kg (wt) 9.1 11.34 Total egg produced 1764 2019 Gross income (Birr) 5292 6057 Net return (Birr) 3017 2870.5 Change in net return 146.5 - Change in total variable cost -911.5 MRR (%) 132.6 90 MRR = marginal rate of return; Birr is Ethiopian currency; the price of the egg during the experiment at local market was 3 Birr/egg; T1= homemade ration; T2 = commercial ration 42 5. DISCUSSION This study investigated the views of people involved in exotic chicken production in kersa district, with respect to the economic importance of chickens, constraints to production, perceptions of disease risk factors and biosecurity measures. For many of the participants in this study, chicken production was not their primary source of income. Although detailed data on participants’ income or assets were not collected we believe that it is likely that almost all exotic chicken producers had very low incomes based on our discussions with the participants, our observations during visits to households and our knowledge of the local area. The RRA approach managed to provide quick over view of exotic chicken production. The RRA method was found to be introductive and boosted the morale of the farmers who were very active and generously offered information that enriched the outcome of the survey. This agrees with the finding by Olwande, (2014) that farmers feel appreciated and become positive, when their ideas are respected. In this study, scarcities of feed were ranked as the most important constraint to exotic chicken production. Poor quality and cost of feed was an important production constraint to farmers in this study area. There are few established feed processing companies in Ethiopia and the majority of them are located near Bishoftu (FAO, 2008). Given that local farmers rank feed constraints so highly; this is a concern for producers in other parts of the country, where the additional transportation costs could be expected to impose further limitations. The result is comparing well with Emmanuel (2015) who reported feed as highly constraints for local farmer. Poor feeding of the indigenous chicken flocks had been reported by several studies including Okeno et al., (2011), Wachira et al., (2010) and Olwande et al., (2010) in Kenya and Yakubu (2010) in Nigeria. Poorly fed chickens always take longer time to reach maturity and produce fewer eggs (Wachira et al., 2010). This partially explains why indigenous chickens take longer time to start laying (6- 10 months) compared to the well fed commercial chickens that start producing eggs at 5 months of age. It also partially explains why the indigenous chickens lay fewer eggs (36- 60 eggs a year) compared to the commercial birds (over 250 eggs a year (Okuthe, 1999)). Well-fed chickens always develop adequate immunity to disease infections (Njagi et al., 2012). Diseases were ranked as the second most important constraint to indigenous chicken production. Emmanuel (2015) in Ethiopia, Wachira et al., (2010) in Kenya and Yakubu, (2010) in Nigeria 43 reported disease as the most important constraint to indigenous chicken production. The other constraints identified in this study were poor animal health services reported by the producers. Poor animal health care associated with indigenous chicken production was the reason for high prevalence of the diseases. Predominantly, this issue identified by informal discussions that followed the ranking exercise about veterinary services. The issues that were identified included difficulty accessing veterinarians, the perception that the treatment was not effective, afraid the cost of their services, Lack of attention to village birds and carelessness of the producers. There was evidence that producers had limited knowledge of the potential benefits of veterinary services, and this may be symptomatic of the previously reported lack of understanding that buyers and sellers of veterinary services in many African countries have of one another (Leonard, 2000). Other important constraints identified such as poor housing, Conflict with neighbor, Inadequate skills, Farmers low attitude theft and predation were also reported by others including Wachira et al., (2010) and Ondwasy et al., (2006) in Kenya and Gondwe and Wollny, (2005) in Malawi and Mohammed et al., (2005) in Sudan. The participants could name and provide accurate clinical signs for some diseases. This is contrary to previous reports (Pagani and Wossene, 2008), where individually and collectively chicken farmers was unable to identify more than a small number of diseases. This difference may be due to the relatively greater importance of chicken production (FAO, 2008; USAID, 2010) and the concentration of animal health expertise in the study area. Many previous studies have focused on Newcastle disease as an important cause of mortality among chickens in Ethiopia (Dessie and Jobre, 2004; Tadesse et al., 2005; Halima et al., 2007) and this is consistent with the opinions of the participants of this study. Coughing and greenish diarrhoea was the most frequently identified disease problem causing bird mortality, and was usually the highest ranked sign of disease. However, our results highlight that a number of other disease were impacting chicken productivity and mortality, including diarrhoea, chronic respiratory diseases, sudden death and reduction in egg production. This study was the first to construct a comprehensive seasonal pattern of major exotic chicken diseases for the district, with a view of providing basis for mitigations for improved productivity. The pattern showed that most exotic chicken diseases occurred during feed scarcity and wet and cold months of the year. All these months are usually associated with stressful conditions that 44 compromise the immunity of the birds. When birds are starved during feed scarcity, their immunity to most diseases is lowered (Wachira et al., 2010). Several studies including Njagi et al., (2012), Njue et al., (2001) and others have shown that stressed birds have poor immune response to infections to the extent that, even less virulent pathogens can cause severe clinical disease in the stressed birds. The producers identified a number of seasonal risk factors, and believed that early morning dew (observed throughout the wet season) was the most important risk factor for disease occurrence. Another seasonal factor included the early onset of the wet season (referred to as early rain or early grass growth). Alternatively, some farmers simply identified the wet season as increasing risk with the greatest magnitude of losses (57.2%) occurring during the rainy season. The result is in contrary with that of (Kusina et al., 2004) that reported the extent and severity of losses were seasonal with the greatest magnitude of losses (47%) occurring during the hot, dry season. In the study area, about 91.7% of the total respondents have encountered most disease outbreak. In Bulbul, Gello and Kitimbille, about 76.1%, 70.2% and 64.4% of households reported that, they have experience of disease outbreak in rainy season in their flock, respectively. The disease affected all classes of chicken and killed all birds. Fast transmitting fatal disease locally named as ‘koksa’ (it means coughing) that may kill all birds in the flock occurs in rainy season. The symptom of the disease was mentioned to be greenish diarrhea and in coordination of movement, which are symptoms of Newcastle disease at different stages. Tadelle (2003) reported that, in five agro ecological zones of Ethiopia, one of the disease commonly reported as ‘Fengle’ that is believed to be Newcastle disease. The farmers identified limited access of veterinary services and resorted to the use of human medicine, which they confessed never worked. Few farmers who vaccinated their birds never followed the recommended vaccination regime making it difficult to control the disease. About 17.2% of the respondents vaccinate their chickens after disease outbreak; this case increases disease severity instead of curing it. This result in line with the finding of (Samson and Endalew 2010) and Fisseha et al., (2010) who reported the level of awareness about availability of vaccines for chicken is low and the farmers do not have any experience of getting their chicken vaccinated 45 against diseases. This is due to the fact that the farmers have no information about disease control and vaccination because of poor extension package of poultry production. The veterinary assistant reported that the farmers were not responsible bringing their chickens on assigned day for vaccination and the problem of dose formation. Housing structures currently used by most households were not appropriate. The housing structures most of which were tiny and sketchy in make (made of pieces of old iron sheets) and some grass house made from locally available material were only used to shelter few birds from hot sun during the day. From the total of 180 chicken owners interviewed, only 145 farmers (80.6%) prepared separate overnight houses for village birds (Table 9). However, the minority (19.4%) of village chicken owners share the same house with their chickens. kept birds on various night sheltering places including; on ceilings of the house (28.6%) and perch inside the hose (73.5%). Lack of attention to village birds, lack of construction materials, lack of knowledge and awareness and shortage of labor and time were some of the major reasons mentioned by village chicken owners for not preparing a separate house for their chickens. Most of the respondents clean their chicken house/shelter daily (76.6%). This result agrees with the survey undertaken by Halima (2007) in northern Ethiopia who reported 74.02 % of the households cleaned their chickens’ house once per day. About 8.5% of the owners cleaned it every two days while (10.6%) of the households clean the chicken’s house once per week. The remaining (1.7%) and (3.2%) clean every 3 to 6 days and never clean respectively. The situation in the other study areas is similar. Lack of frequent cleaning of poultry shelter can easily cause diseases and increase morbidity and mortality rates of chicken. Thus, raising awareness of farmers on the need for cleaning shelters is important that all development practitioners should take seriously. Housing is essential to protect the bird against incremental weather (rain, sun, very cold winds, dropping night temperatures), predators, and theft, and also to provide shelter for hens laying eggs and broody hens. And most important: housing is necessary in order to maintain a high level of biosecurity in the flock. Furthermore, a suitable or comfortable poultry house is extremely important to maintain an efficient production and for the convenience of the poultry farmer. The major feed resource base for rural poultry in this study area is scavenging and it consists of anything edible found within the environment. The chickens got most feed requirements from 46 scavenging; around the home stead, where they could eat plant leaves and seeds, insects and any other edible within range. This scavenging feed resource base (SFRB) can include household waste, grains, worms and insects, grasses and many more. The birds got plenty of food during harvesting seasons; in October, November and December each year. The birds lived mainly on scavenged food during the other months of the year, except in some few households where they received little quantities of grains and kitchen left over as supplements, but inconsistently. The SFRB is not constant but changes with season and household farming activities, for example, sowing and harvesting. According to Tadelle (1996), protein supply may be critical, particularly during the drier months of the year, whereas energy may be critical during the rainy season. To improve the nutrition of village chickens, and hence productivity, supplementary feeding may be necessary as this will reduce pressure on the available SFRB. This will increase the biomass that can be supported by the system, reduce survival pressure and selection against the weakest members of the flock and hence reduce mortality of chicken’s due lack of adequate nutrition. Lack of adequate nutrition predisposes chickens to the effects of diseases. Farmers interviewed in the survey realized the benefits of supplementary feeding to their poultry. The most commonly used supplementary feeds in the study area include maize, Dura and kitchen leftovers. Frequency of feeding and amounts were variable and depended on seasonal supply and fluctuation in local feed resources such as cereal grains. In addition, poultry feed is expensive for the rural resource-poor farmers to purchase (ARC 1999). Improved feeding will also improve productivity, well fed birds are resistant to most infections and hence deaths from diseases will go down. This was demonstrated by the seasonal patterns of diseases constructed in this study. Low or no major disease prevalence was shown to be occurring in the harvesting months with plenty of food for the chickens. That being the case, it is important to develop feeds based on locally available ingredients to supplement the SFRB of rural chickens. A very high level of mortality reported in non-supplemented birds. Despite variations in source of water and frequency of watering, almost all of the respondents provided water for their chickens. This is a promising and good experience and could be considered as one aspects of their concern to their chickens. Households reported use of different sources of water for chicken and river is the major source of water followed by pond water and borehole 47 water. In the Kitimbille and Gello villages, the majority of chicken owners used river water sources 86.7% and 59.6% respectively. This result was in agreement with that of Mekonen (2007) who reported that water for chickens in Southern Nations, Nationalities and Peoples Regional State of Ethiopia was drawn from river (37%), pond (35%) and bore hole (28%). The most widely used types of watering troughs in the study area were broken home utensils, plastic material and locally made watering trough from wood. Regarding the frequency of cleaning watering trough, 69.4% of chicken owners never clean watering trough, 6.1% clean twice per week, 21.1% once per week and 3.3% daily (Table 11). However, more than half of chicken owners having watering trough responded that they never cleaned watering trough, which indicates poor sanitation. The result is in line with the finding of Abdurehman (2014) that reported the frequency of cleaning watering trough, 47.8% of chicken owners never clean watering trough, and 23.3% clean twice per week, 16.7% once per week and 12.2% daily. This finding disagrees with the report of Tesfau (2007) who reported the container was not washed at all in 15.3 percent of the households; simply poured extra water so as to remove the dirt seen in the container around the village of Dire dawa town. The overall prevalence of ectoparasites in the study area during the survey was 76.7%. The observed overall prevalence of 76.7% of ectoaparasite infestation in the current study is slightly comparable to 70.73% report from Meerut Kansal and Singh (2014). However, higher prevalence rate of 91.5% Belihu et al., (2010), 86.67% Shanta et al., (2006) and 100% Bala et al., (2011) were recorded in East Shoa zone (Ethiopia), Bangladesh and Nigeria, respectively. This discrepancy might have occurred due to agro-ecology, management, climatic factors in the study area and sample size and differences in sampling time. In the present survey, a significantly (p<0.05) higher overall prevalence of ectoparasite was found in scavenging only chickens (96%) slightly comparable finding of 93.68% was reported in central Ethiopia (Hagos Ashenafi and Eshetu Yimer 2005) and Hossein et al., (2012) Sistan region, east of Iran who reported prevalence of (93.22 %). In supplemented birds the prevalence was 56.7% slightly comparable finding of 48.21% was reported in and Around Ambo Town, Ethiopia Firaol et al., (2014) (Table 16). Ectoparasite infestation in feed type showed significant difference (p<0.001) with odds ratio (OR) of 22; where scavenging only chickens were 22 times more likely 48 infested than scavenge with supplemented chickens. The likely explanation is that it might be associated with long period exposure to the infested environment than the supplemented chickens, hence a higher prevalence rates. In addition, scavenging only chickens scavenge through a wider area of the farmers’ homesteads and beyond that make them more exposed to the source of infestation. The species of ectoparasites registered in present survey includes, M. gallinae which had the highest prevalence 46.7% followed by, C.mutans, Echidenophaga gallinacean, M. stramineus, A. persicus D.gallinae, and C. heterographus with a respective prevalence of 40%, 33.3%, 30%, 26.7%, 23.3% and 16.7%, respectively. These ectoparasites have commonly been identified in free range chickens (Ekpo et al., 2010). Lices were more prevalent comprising 73.3% of the total infestations. Lawal et al., (2016) reported lices as the most prevalent ectoparasite in village chickens. Other studies reported lower prevalence of lice, ranging from 8.1%-19.5% (Hagos Ashenafi and Eshetu Yimer, 2005; Solomon Mekuria and Elsabet Gezahegn, 2010). The higher prevalence of lices in the current study areas might be associated with poor hygiene of chicken houses as well as lack of control measures towards such parasites or may be related to favourable climatic condition for the successful breeding and development of the parasites in the study areas (Hopla et al., 1994). Their fecundity is high and they lay relatively large number of eggs in clusters (Urquhart et al., 1996). Besides, after introduction into a flock the lice can spread from bird to bird very rapidly by contact. Perhaps these factors contributed towards a higher prevalence of lices in backyard poultry. Out of the three lice species, M. gallinae had the highest prevalence 46.7% followed by, M. stramineus, in scavenging only chickens. Similar finding has reported by (Solomon Mekuria and Elsabet Gezahegn (2010) and Lawal et al., (2016). Menacanthus stramineus (30%) was the second most prevalent species of lices encountered in this study. The result was slightly compareable to the finidng of Eslami et al., (2009). Lower prevalence of (1.28%) M. stramineus was reported by (Firaol Tamiru et al., 2014). However, highest prevalence of 70% and 65.33% M. stramineuswas reported from Bangladesh Shanta et al., (2006) and Ethiopia Belihu et al., (2010), respectively. M. stramineus is the most 49 pathogenic species of poultry lice as it causes severe anaemia by puncturing small feathers and feed on blood that oozes out. It is known to cause inflammation of the skin and extensive scab formation (Urquart et al., 1987). Cuclotogaster heterographus 16.7% the third lice species identified in this study. The prevalence was lower than the report of (40%) Solomon Mekuria and Elsabet Gezahegn 2010 Wolayta Soddo town, Southern Ethiopia. Mite infestation was registered as the second most in this survey in contrary to Tesfaheywet Zeryehun and Yonas Yohannes (2015) who reported mites as the first most prevalent (26%). Cnemidocoptes mutans (40%) is slightly comparable to the finding of Firao et al., (2014) but lower prevalence of C.mutans 9.4% Bala et al., (2011) and 0.89% Biu et al., (2012) reported in Nigeria. The difference might be due to management, climatic and geographic (altitudinal) difference among these studies. C. mutans is one of the dozen related species of scaly leg mites occurring on various chickens (Swai et al., 2010). The second species of mite observed in the current survey was D. gallinae (23.3%). Dermanyssus gallinae (23.3%) encountered in this study was lower than 71.2% and 57% reported by Zumani (2011) and Shanta et al., (2006). Dermanyssus gallinae is a common mite of poultry houses and the most important haematophagus ectoparasite of birds (Eslami et al., (2009). Dermanyssus gallinae (northern fowl mites), apart from causing intense pruritis and pain act as vectors of Borrelia anserine (Urquart et al., 1987). Chicken mites may cause severe problems for producers, through potential direct effects on weight gain, egg production and sperm production in rooster and importance nuisance pest for human beings including poultry workers and particularly who handle hens and eggs (Bellanger et al., (2008). Echidnophaga gallinacean is the only flease encountered in the current survey with a prevalence of 33.3%. This finding is in line with the finding of Lawal et al., (2016) in Gombe, Northeastern Nigeria. The Prevalence of (33.3%) encountered in this study is lower than 50.7%; 71.9%; 44.4% and 51.16% reported by Moyo et al., (2015), Mukaratirwa and Hove (2009), Firaol et al., (2014) and Belihu et al. (2010) respectively, but higher than 9.4% and 0.89% reported by Bala et al., (2011) and Biu et al., (2012). Fleas have been reported as the dominant ectoparasites by Nnadozie (1996) while Saidu et al., (1994) showed that they were the least occurring of the ectoparasites. Moreover, Adene (1975) encountered no flea in their survey of blood and ectoparasites of domestic 50 fowls in Ibadan, Western Nigeria. We speculate that these variations in result could be attributed to the season, time of the day, and the study location with respect to urban, periurban or village setting. Moreover, it is expected that the prevalence may have been higher as other flea types may have left the host after feeding and during overnight caging of the chicks. A. persicus was the only tick species identified with prevalence of (26.7%). This finding was higher than the findings of Bala et al. (2011), Mulugeta et al., (2013), Mukaratirwa and Hove (2009), Kelay Belihu et al., (2010) and Tesfaheywet and Yonas, 2015 who recorded 8.8%, 4.97%, 5.2%, 5.2% and 1.3% respectively. However, Bunza et al., (2008) reported 62.2% prevalence of Argas persicus in village chickens in a survey to study the ticks in domesticated birds which was higher than the current finding. Considering the respective findings reported in the various works, the difference might be due to the numbers of birds examined during various study, type of management system practice, climatic and geographic (altitudinal) difference among the various study areas. However, this does not reflect the true figure, due to the feeding habit of the parasite where it briefly visits the host usually at night; hence it may not be found abundantly at sampling during the day time. The result of this survey also showed a wide range of gastrointestinal parasitic infections among backyard village chickens. The overall prevalence of gastrointestinal parasites was 62.2%. This finding is comparable with some reports 63.6% by Ogbaje et al., (2012) in Makurdi and 59.64% by Yehualashet (2011) in Ethiopia. In scavenging, African chicken’s even higher prevalence (99- 100%) of helminth infections reported Mwale and Masika, (2011). The observed higher prevalence of helminth infection in scavenging chickens could be due to a constant contact with the infective stage and/or intermediate host. Or it could be the absence of chickens deworming practice by the owners Mekibib et al., (2014). The prevalence was higher in scavenging only chickens (76%). This finding is in a general agreement with the report of various investigators from Ethiopia and other areas. Such frequent multiple species infestations could be explained by the free roaming nature of the scavenging chickens, which increase the access to different types of embryonated parasite eggs or infective larvae. Moreover, in the absence or scarcity of feed these chickens could be forced to eat different 51 insects, snails, slugs, dung beetles and earth worms, which are believed to be the intermediate hosts of some nematode and cestodes. The most prevalent nematode species encountered in the present survey were Heterakis gallinarum (40%) and Ascaridia galli (26.7%), Raillietina spp. (26.7) while Capillaria spp. (13.3%) was the least frequently recorded. Previous studies indicated high prevalence rates of Ascaridia galli (45- 67%) and Heterakis gallinarum (11-43%) in backyard chicken from different parts of Ethiopia Abebe et al., (1997), Bersabeh (1999), and Teshome (1991) and Alam et al., (2014) from Bangladesh. Higher prevalence of Heteraki sgallinarum 93.3% reported by (Rahman et al., 2009) among nematode spp. in Malaysia. (Baboolal et al., 2012) reported lower prevalence of (0.9%) Heterakis gallinarum. This discrepancy could be related to the differences in the management systems, control practices in the farms and seasonal differences. It has been reported that these factors exacerbates the infection of domestic birds in the tropics (Opara et al., 2014).The presence of Heterakis gallinarum poses the danger of enhanced transmission of Histomonas meleagridis to both susceptible turkeys and other poultry through shedding of the eggs in the environment. Ascarida galli (26.7%) was the second prevalent nematode parasite encountered in this study. this species had been reported in several studies as the commonest and most important helminth infection of poultry (Ahmed et al., 2011). The result was in line with the report of Yacob and Hagos (2013). In previously conducted studies higher prevalence of Ascarida galli ranging between 47 to 67% (Asfaw, 1992; Teshome, 1993; Abebe et al., 1997; Bersabeh, 1999; Hagos, 2000) were reported in different parts of Ethiopia. But lower prevalence (5.8%) with (Vandana et al., 2012) was reported in commercial broiler chickens. The rate of infection by A.galli was higher in the lowland and midland areas compared to the highlands. These variations could be due to differences in local environmental conditions, which support larval development and facilitate transmission. About the cestodes Raillietina spp. were the most prevalent one which found in (26.7 %) in the current survey (Table 18). Similar to this finding in Bangladesh, Alam (2014) reported prevalence of Raillietina spp. (21.05%) during winter season. Higher prevalence of Raillietina spp were reported by (Ahmed et al., 2011) in Eritrea and (Eslami et al., 2009) in Iran with the prevalence rate of (82.35%) and (60%) respectively. The relatively higher prevalence of Raillietina spp. can 52 be attributed to the wide spread and eases accessibility of intermediate hosts (dung beetles, ants) to the scavenging chickens. The habit of free range chickens of scratching any material including cow dung to look for among other things maggots accounted for the high prevalence of Raillietina whose intermediate hosts are maggots of Musca domestica (Dube et al., 2010). The difference in the coverage of study areas might have strongly influenced the differences in the diversity of species recorded, since the current study covered only 1 district compared with more than one districts in the other study. Also, Capillaria spp. 13.3% (6/45) was other recorded nematode species in the current survey. This study agreed with (Trisha et al., 2014) reported prevalence (4.68%) in indiginous scavenging chickens. The lower Mean faecal egg counts (FECs) were recorded in the scavenging with supplemented chickens as compared to the scavenging without supplementation. These findings are in agreement with the work by Idi et al. (2007).The low EPG in the scavenging with supplemented group in the present surveymight be related to both the low number of female worms and the low fecundity. It also might be related to the immune status of the supplemented birds as the commercial feed contained vitamin A. the low worm burden in the supplemented group can partly be related to the immune status of the supplemented birds(Villamor and Fawz, 2005). The results of the laboratory analysis of the different feed ingredients used and the experimental rations are given in Table 19. From the analysis result, it can be seen that soybean meal (SBM) and noug seed (NS) are rich in CP content that make these ration ingredients to be a good source of protein supplement for poultry. Previous reports indicated that the CP content of SBM to be in the range of 41 to 50 % (Eekeren et al., 2006 and Waldroup, 2002). However, similar to the value noted in this study, a 38% CP content of SBM was reported in Ethiopia (Aregaw, 2010) and (Senayt 2011). The difference in the CP content of SBM might be due to various factors such as differences in variety, origin and the method of oil processing that the byproduct is obtained. The CP content of the NS used in the current experiment is slightly comparable to 29.2 and 28.9% CP reported by (Senayt 2011) and Fantie and Solomon (2008), respectively. Values for the CP and ME content of maize grain used in the current experiment were comparable with that reported by Mesert (2006) and (Senayt 2011). 53 The DM and CP content of wheat short used in this study were slightly similar to the 90.7% DM and 15.4 % CP values reported by Haftu (2012). The CP content of the treatment diets were between 16.45% and 17.8%, which was within the range of CP requirement (14-19%) suggested by Leeson and Summers (2001) for layers. Similarly, Tadelle (1997) noted that the protein requirement of high producing laying hens should be between 16-18% of the diet to meet the needs of egg production, maintenance and growth of body tissues. The ME content of treatment diets in homemade feed slightly lower than the commercial feed and was slightly greater than the anticipated 2800 kcal/kg DM ME. This appeared to be mainly due to the greater ME content of SBM. Ration in homemade feed contained relatively greater Crude Fat content due to relatively more level of CF in NS and SBM. The low intake of DM recorded in homemade feed supplemented chickens may be due to higher crude fiber content of the diet (7.2%), which is almost close to the maximum limit (10% CF) for poultry. Xiohe (2010) showed that leghorn chickens fed ration consisting above 10% CF in the diet cannot maintain the required metabolic energy intake and consequently growth of the chickens is reduced. In line with these findings, (Senayt 2011) reported that an increase in CF content in the diet of poultry causes a reduction in digestibility of nutrients and therefore intake. The slightly low DM intake in homemade feed could be also related to differences in the amino acid profile of the two protein supplements. Since animal source protein that used in commercial feed consists higher total and digestible amino acid content as compared to homemade feed that used plant protein sources (Campbell 2016), its inclusion at appropriate level might have provided the bird with better balanced amino acid than the ration containing plant source protein, which could also be the reason for the variation in the DM intake among treatments in this study. This may be due to differences in the palatability of diets formulated for the two treatments because of the fineness form of homemade feed used in this study. The ration used in the present experiment was mash form, and since the particle size of ration was very small (almost powder) the animals had difficulty in consuming more of the ration because of no appropriate grain milling masion. Although initial body weights (BW) were slightly similar (P > 0.05) among treatments, the mean BW gain of the birds for the entire experimental period was variable (P < 0.05) among treatments (Table 20). The BW gain was greater for birds in commercial supplemented as compared to the 54 ones in homemade supplemented. The lower body weight gain in homemade supplemented chickens might be due to the lower profile and/or levels of amino acids supplied; and partly may be associated with the low level of feed intake. Wu et al., (2001) noted that deficiency of essential amino acids affects feed consumption and growth in chicken. Animal source protein is better than plant source protein in amino acid composition and profile, especially in lysine content and is also better in nutrient digestibility due to the less fiber content. This might have resulted to significantly better nutrient supply and improvement in growth rate of birds when commercial feed is supplemented. Hassan et al., (2000) reported that hens fed high protein diets had higher final body weight than those fed lower protein diets. Similarly, El-Sayed et al., (2001) noted that body weight of birds fed diets with high protein levels were significantly heavier than those fed diets of high energy level. Differences in BW gain in the present study might also in part be attributable to the slight differences in CP and ME content of the treatments rations. This suggestion is supported by the results of BW gain reported by Haitham (2010) that noted decreased BW because of dietary protein reduction from 17 to 14% in treatment rations. Total egg produced per bird and HDEP was greatest (P < 0.05) for commercial supplemented birds, and slightly lower for homemade supplemented birds (Table 20). The overall mean HDEP for homemade supplemented birds (68.23±1.34%) in the present experiment were lower than the mean for commercial supplemented birds (76.87±1.03%). The lower egg production in homemade supplemented birds noted in this study might be associated with the lower DM intake and possibly lower content of CP of homemade supplemented bird’s diet that might have limited nutrient supply for egg production. Similarly, the highest egg production in commercial supplemented birds was due to greatest DM intake by birds and could also be due to the improvement in balanced nutrient supply due to the added premix in the diet. Onwudike (1981) noted that reduction in hen- day egg production was associated with amino acid deficiency and imbalance. The rate of lay observed in this experiment for homemade supplemented birds higher than the report of (Senayt 2011) that reported the overall mean HDEP (58.5±1.22%). The authors reported 92.4% and 73% HDEP and HHEP, respectively. Such differences might have been due to strain and breed purity difference. Feed conversion ratio did not differ among treatments. The values obtained here were above the optimum value of 2.0 for layer noted by Peter (2011) but were however better than the range (4.0- 55 5.0) reported by Haftu (2012) and Uko and Kamalu (2008). Hirnik et al., (1977) also showed that the variation in feed conversion efficiency is highly dependent on the number of eggs produced (by 51%) followed by feed consumption (31%) and egg weight (18%). Since the variation in egg weight and feed consumption between treatments is not wide, large difference in feed conversion ratio is also not expected. Results of the experimental study indicated that the chicken were infected with Ascaridia galli, Heterakis gallinarum, Eimmiria species and Raillietina tetragona. Levamisole HCL at a dose of 30 mg/kg body weight reduced FECs in the first round. No appreciable difference in FECs was noticed up to 3rd period of experimentation in the two groups of chickens. The probable reason was that effect of the anthelmintic medication of the chickens prior to start of the experiment might have played some role in preventing the establishment of the worms. Results showed decreased in EPG after treatment with Levamisole 30mg/kg, this agree with results of (Begum et al.,2012) which showed significant decrease in EPG , due to decreased in numbers of infested worms in alimentary tract , the decrease occur due to anthelelementic activity of Levamisole in which agonist nicotinic receptors and elicit spastic muscle paralysis due to prolonged activation of the excitatory nicotinic acetylcholine receptors on nematodes body wall muscles (Akhtar et al.,1985) . An increase in faecal egg count in the second round might be due to that levamisole has no residual effect unless delivered using a slow-release device. This means that a single administration will kill the parasites present in the host at the time of treatment, but it will not protect the host against re-infestations. Levamisole is not ovicidal, which means it will not affect eggs already present, but it will affect the larval stage of the worm or the chickens may probably ingest an intermediate host or paratenic host houseflies that are considered paratenic hosts, for some nematode as they can ingest the egg in feces and a juvenile may hatch in tissues, which stays dormant until eaten by birds or may ingested the egg in contaminated water as running water (which parasite tranismit with running water) provided (Permin and Hansen 1998). A significant increment in the EPG was recorded in the homemade feed supplemented chickens after 3rd period whereas, in commercial feed supplemented groups the FECs remained unchanged throughout the 90 days experimental period (Figure 5). The low worm burden in the commercial 56 feed supplemented group can partly be related to the immune status of the commercial supplemented birds (Villamor and Fawz, 2005). Because commercial feed contains premixes that contain vitamin A, there are lines of evidence that vitamin A improves immunity, thus when birds are supplemented they develop strong immunity that interferes with establishment of the worms and also lowers fecundity of the fertile female worms (Idi et al., 2007). In addition, vitamin A improves the integrity of the intestinal mucosa and hence interfering with both the migration phases of the larvae and the attachments of the adult worms to the mucosa (Crompton and Nesheim, 2002). These perhaps interfere with the overall establishment of the worms. Chickens fed on diets containing animal protein acquire fewer worms compared with those fed mainly on plant protein. Increasing levels of essential amino acids especially, lysine and calcium, in feed also lessens the number and length of parasite Hafiz et al., (2015). Furthermore, feed rich in vitamins A and B minimized the chances of worm establishment in the intestine (Walker & Farrell, 1976). GI parasitism increases the amino acid requirement of GI tissues and consequently peripheral tissues are denied the nutrients required for optimal growth. Normal animal performance in the face of larval challenge may be possible if the protein supply is increased, and this is possible by balanced feed supplementation, which improves the bio-availability of proteins. (Dey et al., 2008; Dubey et al., 2012). The economic return in terms of partial budget from birds under different treatments is presented in Table 26. The highest value for marginal rate of return was recorded in homemade supplemented chickens. According to partial budget analysis, hen fed commercial feed returned a higher total net income than homemade supplemented chickens. Even though, layers in hen fed commercial feed showed higher percentage of egg production, it was not found economically feasible as compared to layers in homemade supplemented. This means, the income obtained from hen fed commercial feed returned less per unit of expenditure, suggesting homemade supplemented to be the treatment of choice in terms of profit. Therefore, substitution of commercial feed with homemade feed is profitable because of the highest value for marginal rate of return. Thus, homemade feed can be substituted for commercial feed economically without affecting body weight, egg production and 57 health status of chickens. However, if cost of locally available ingredients changed, recommendation could change based on the existing conditions for egg production. 6. Conclusions and Recommendations 6.1 Conclusions The survey was conducted to identify exotic chicken constraints in Kersa district on chickens those fed supplementary feeding given by the LIVES project and left as scavenging only. The exotic chicken production suffers from the scarcity of feed, diseases, unavailability of reliable veterinary and extension services, inadequate knowledge and skills in the management of exotic chickens amongst the farming community. Infestation of chickens with ectoparasites and endoparasites were important constraint observed in the study area. To tackle these problems and improve 58 production performance with lower cost, a feeding experiment on layer chickens was conducted using feed concentrates from commercially purchased feed and homemade from locally available ingredients. Parasitic infections in chickens induce low levels egg production and body weights. Supplementation of commercial feed significantly improved egg production, body weight gain and total profit but when feed cost is taken in consideration lower profit than homemade feed. The effects of parasitic infection on the above-mentioned parameters can be reversed by supplementing the chickens with mixed feed especially commercial feed as it contained premixes that contained vitamin A eventhough it has lower profit as compared to homemade feed. Vitamin A moderates parasitic infection in chickens particularly by reducing egg counts and fecundity of fertile female worms. Vitamin A has, therefore, beneficial effect on moderating the effect of parasitic infestation. Higher profit will expected by using premixes with homemade feed. 59 6.2 Recommendations It is recommended that extension packages that would enhance the knowledge and skills of the exotic chicken farmers on exotic chicken production, be initiated and sustained for the improvement of the productivity of the birds. Given the current low interaction between the veterinary profession and chicken producers, efforts to increase chicken health and production through veterinary input may need to include both improved training for veterinarians and efforts to demonstrate the benefits of veterinary input to farmers and veterinarians alike. Animal-health technicians may also be able to make an important contribution to provision of preventive health care. The evidence on the effects of parasites on the above-described parameters necessitates strong measures to control helminths infestations in chickens. Similarly, considering the benefits of vitamin A described above supplementation of homemade feeds with vitamin A is recommended to be a routine practice for farmers raising chickens for various purposes. The above-mentioned recommendations are particularly important for free range chickens, which are usually highly infested by worms and depend on natural sources of vitamin A, which vary significantly with seasons. While the present study reports association of different concentrate with performance and parasitic infestation under experimental condition, it could be worth to have similar study conducted in the field in order to provide an insight of what happens in a typical field condition, where feed and parasites were a problem. In the present study, it is not clear whether the reduction in FEC was due to reduced worm load or due to decreased fecundity of GI nematodes. Nevertheless, the present findings have got tremendous implications on the epidemiology of infection. Reduced FEC means less contamination of the environment with infective larvae, which in turn results into less infection in the chickens scavenge on the area. Moreover, the mean FECs of the treated groups were much lower, which warrants for anthelmintic medication. Thus, the frequency of medication can be curtailed in the balanced fed and good management. This is especially important in the study area where inadequate veterinary service is raised as the constraints. 60 REFERENCES Abdelqader, A., Wollny, C. and Gauly, M. (2007): Characterization of local chicken production systems and their potential under different levels of management practice in Jordan. Trop. Anim. Health Prod., 39:155-164. Abdurehman, A. (2014): Study of production practices, fertility, hatchability and egg quality of rural chicken in gorogutu district, eastern Hararghe, Ethiopia. MSc. Thesis, Haramaya University. Abebe, W., Asfaw, T., Genete, B., Kassa, B., Dorchies, P.H. (1997): Comparative studies of external parasites and gastrointestinal helminthes of chickens kept under different management system in and around Addis Ababa (Ethiopia). Rev. Med. Vet. 148: 497-500. Aberra, M., Maak, S.and von, G. (2005): The performance of naked neck and their F1 crosses with Lohmann White and New Hampshire chicken breeds under long-term heat stress conditions. Ethiop. J. Anim. Prod., 5: 91-106. Abraham, L. and Yayneshet, T. (2010): Performance of exotic and indigenous poultry breeds managed by smallholder farmers in northern Ethiopia. Lives. Res. Rural Dev., 22: 10-13. Adene, D.F., Dipeolu, O.O. (1975): Survey of Blood and Ectoparasites of Domestic fowls in Ibadan, Western State of Nigeria. Bulletin of An. Health and Prod. in Afr. 23:333–335. Adriana, G., Loredana, P. and Vasile, C. (2013): Prevalence and distribution of Eimeria species in broiler chicken farms of different capacities. Volume 20. https://doi.org/10.1051/ parasite. Ahlers, C., Alders, R., Bagnol, B., Cambaza, A., Harun, M., Mgomezulu, R., Msami, H., Pym, B., Wegener, P., Wethli, E. and Young, M. (2009): Improving village chicken production: A manual for field workers and trainers. ACIAR Monograph No. 139. Australian Centre for International Agricultural Research, Canberra. http://www.aciar.gov.au/publication/MN139. Ahmed, A. A., Olfat, A. M., Aida, A. N. and Mohamed, S. A. (2012): Studies on Coccidia of Egyptian Balady Breed Chickens. Life Sci Jour. 9(3): 14. 61 Ahmed, M. and Singh, P. (2007): Estimates of genetic parameters for some economic traits in White Leghorn. Indian Poult. Sci., 42:311-312. Alam, M. N., Mostofa, M., Khan, M. A. H. N. A., Alim, M. A., Rahman, A. K. M. A. and Trisha, A. A. (2014): Prevalence of gastrointestinal helminth infections in indigenous chicken of selected areas of Barisal, Bangladesh. Bangladesh Journal of Veterinary Medicine, 12(2): 135-139. Alem, T. ( 2014): Production and reproduction performance of rural poultry in lowland and midland agro-ecological zones of central Tigray, Northern Ethiopia.Afr. J. Agric. Res.,9: 3531- 3539. Alexander, D.J. (1997): Newcastle disease and Avian paramyxovirus infections. In: Calnek, B.W., Bernes, H.J., Beard, C.W., McDougald, L.R., Saif, Y.M. (editors), Disease of Poultry, 10th edn. Iowa State University Press, Ames, USA, Pp: 541-569. Alganesh, T., Matewos, B. and Gizaw, K. (2003): Survey on traditional livestock production system. Proceeding 11th Annual Conference of Ethiopian Society of Animal production, Addis Ababa, Ethiopia, August 28-30, 2003. pp. 141-150. AOAC (Association of Official of Analytical Chemists), 1990. Official methods of analysis, 15th ed. Arlington, VA. Association of Official Analytical Chemists. Pp: 957. ARC (Agricultural Research Council) 1999. Five-Year Rolling Strategic Plan (1999–2004). Ministry of Lands and Agriculture. Aregaw, A.(2010): Comparative Study of Laying Performance, Egg quality, Fertility and Hatchablity of Hrro, Koekoek and Lohmann silver chicken under intensive managment. Asfaw, T. (1992): A survey of ectoparasites and gastro-intestinal helminthes of chicken kept under different management system in and around Addis Ababa. DVM thesis, Faculty of Veterinary Medicine, Addis Ababa University, Ethiopia. Pp: 22. 62 Ashenafi, H. (2000): Survey of identification of major Diseases of local chickens in three selected agro climatic zones in central Ethiopia. DVM thesis, Addis Ababa University, Faculty of Veterinary Medicine, Debre Zeit, Ethiopia, pp: 1 -60. Ashenafi, H. and Yimer, E. (2005): Ectoparasites of local scavenging chickens of central Ethiopia. Ethiop. J. Sci.,28 (1); 69 - 74. Baboolal, V., Suratsingh, V., Gyan, L., Brown, G., Offiah, N. V., Adesiyun, A. A. and Basu, A. K. (2012): The prevalence of intestinal helminthes in broiler chickens in Trinidad. Vet.Arhiv.82: 591-597. Bala, A.Y., Anka, S.A., Waziri, A., Shehu, H. (2011): Preliminary Survey of Ectoparasites Infesting Chickens (Gallus domesticus) in Four Areas of Sokoto Metropolis. Nigerian Jour. of Basic and Applied Sci. 19: 173-180. Begum, M.M., Akgr, A., Hassani, M. F., Barman, V.G., Ali, A.A. and Mian, R. (2010): Comparative efficacy of leaves extract of Neem and Biskatali with patent drugs Piprazine and Levamisole against Ascaridiasis of endogenous chicken. Int. J. Bio Res. 1(5):17-20. Belihu K., Mamo A., Lobago F., Ayana, D. (2010): Prevalence of Ectoparasites in Backyard Local Chickens in Three Agroecologic Zones of East Shoa in Ethiopia. Revue Méd. Vét: 160: 537-541. Belihu, K., Mamo, A., Lobago, F., Ayana, D. (2010): Prevalence of ectoparasites in backyard local chickens in three agroecologic zones of east Shoa in Ethiopia. Revue Méd. Vét: 160: 537-541. Bell, D. and Weaver, W. (2002): Commercial chicken meat and egg production. 5thed. Cambridge, Massachusetts. Kluwer Academic Publisher. Bellanger, A.P., Bories, C., Foulet, F., Bretagne, S. & Botterel, F. (2008).Nosocomial dermatitis caused by Dermanyssus gallinae.Infection Control and Hospital Epidemiology, 29: 282–283. Benvenuti, M., Lorella, G., Carlo, P., Domenico, G. and Marco, B. (2012): Buckwheat bran (Fagopyrum esculentum) as partial replacement of corn and soybean meal in the laying hen diet. Ital. J. Anim. Sci., 11:2. 63 Bersabeh, T. (1999): A survey of ectoparasites and gastro-intestinal helminthes of backyard chickens in three selected agro climatic zones in Central Ethiopia. DVM thesis, Faculty of Veterinary Medicine, Addis Ababa University, Ethiopia. Pp: 2. Biu, A.A., Ahmed, H.A., Konto, M., Paul, B.T. (2012): Survey of podoknemidokoptiasis in locally domesticated market chickens (Gallus gallus domesticus) in Maiduguri, Nigeria. J. of Med. and App. Biosci. 4: 39-4. Bunza, M.D.A., Yahaya, M.M., Muhammed, A.S., Saidu, A.R. (2008). A Survey on Ticks Species Infesting Domestic Birds Sold at Sokoto Central Market. Sokoto J. Vet. Sci. 7(2):56 – 58. Bush, J. (2006): The threat of avian flu predicted impacts on rural livelihoods in southern Nation, nationalities and peoples region (SNNPR), Ethiopia. The Food Economy Group, May 2006. CACC (Central Agricultural Census Commission), (2003): Statistical report on farm management practices, livestock and farm managments Central Statistical Authority report of 2004- 2005, Vol. II, Addis Ababa, Ethiopia. Caston, (2008): "Infectious Bursal Disease Virus (IBDV)". Segmented double-stranded RNA viruses: Structure and molecular biology. Caister Academic Press. ISBN 978-1-904455-21-9. Catley, A. (2006): Use of participatory epidemiology to compare the clinical veterinary knowledge of pastoralists and veterinarians in East Africa. Tropical Animal Health and Production, 38: 171- 184. Christensen, J.P., Olsen, J.E., Hansen, H.C. and Bisgaard, M. (1992): Characterization of Salmonella enterica serovar Gallinarum biovars gallinarum and by plasmid profiling and biochemical analysis. Avian Pathol, 21: 461-470. CIRAD, (2005 CIRAD, (2005): Investigation of poultry diseases in Ethiopia. Mission report, the French Agricultural Research Centere for International Development (CIRAD), 29 June-7 July 2005, Pp: 6-14. 64 Crompton, D.W.T. and Neisheim, M.C. (2002): Nutritional impact of intestinal helminthiasis during the human life cycle. Annual Review Nutrition 22: 35-59. CSA. (2011): Agricultural sample survey report on livestock and livestock characteristics (prevent peasant holdings). Statistical Bulletin 505, Addis Ababa. 2: 1-21. Daniel, W.W. (1999): Biostatistics: a foundation for analysis in the health sciences. 7th ed. New York. Dawit, A., Tamrat, D., Stotaw, F., Nzietcheung, S. and Roy, D. (2008): Overview and background paper on Ethiopia’s poultry sector. Relevance for HPAI Reseasrch in Ethiopia. www.hpai-reserach net. Demeke, S., (2004): Egg production performance of local and White Leghorn hens under intensive and rural household conditions in Ethiopia. Lives. Res. Rural Dev., Vol. 16. Demeke, S., (2007): Suitability of hay-box brooding technology to rural household poultry production system. Lives. Res. Rural Dev., Vol. 19. Dessie, T., Jobre, Y. (2004): A review of the importance and control of Newcastle disease in Ethiopia. Ethiop. Vet. J. 8:71–81. Dey, A., Dutta, N., Sharma, K., Pattanaik, A. K., (2008): Effect of Dietary Inclusion of Ficus infectoria leaves as a protectant of proteins on the performance of lambs. Small rum. res. 75: 105- 114. Dinka, H., Regassa, C., Fufa, D., Endale, B. and Leta, S. (2010): Major constraints and health management of village poultry production in Rift Valley of Oromia, Ethiopia. American-Eurasian Jour. Agric. Environ. Sci., 9 (5): 529-533. Doviet, M. (2005): Effect of supplementation, breed, season, and location on feed intake and performance of scavenging chickens in Vietnam. PhD thesis, Swedish University of Agricultural Sciences. Pp: 45. 65 Dube, S., Zindi, P., Mbanga, J. and Dube, C. (2010): A Study of Scavenging Poultry Gastrointestinal and Ecto-parasites in Rural Areas of Matebele land Province, Zimbabwe. Inter. J. Poul. Sci., 9(9): 911- 915. Eekeren, A.N., Maas, H.W., Saatkam, p.and Verschuur, M. (2006): Small Scale Chicken Production. Digigrafi, Wageningen press, Netherlands Pp. 99. Ekpo, U. F., Ogbooye, A.A., Oluwole, A.S. and Takeet, M. (2010): A preliminary survey on the parasites of free range chicken in Abeokuta, Ogun state, Nigeria. J. Natural Sci. Eng. Tech., 9, 123-130. El-Sayed, A. N., Rizk,R.E., El-Deen,M. B. and Hedaia,M.(2001): Effect of strain and dietary regimen on the performance of local chickens. Egypt Poult Sci, 21:1021-1038. Emmanuel, L. (2015): Assessments of commodity and trade issues made by USDA staff and not necessarily statements of official U.S. government policy to increase broiler production by approximately eight percent to 4.2 million tons on rising demand from the growing middle class. Poultry and poultry products annual 2015, gain report number: IN5124 New delhi, India. Erickson, G. A., Mare, C. J., Gustafson, G. A., Proctor, S. J., Miller, L. D. andCarbey. E. A. (1977): Interactions between viscerotropic velogenic Newcastle disease virus and pet birds of six species. Clinical and serological responses and viral excretion.Avian Dis. 21:642–654. Eslami, A., Ghaemi, P., Rahbari,S. (2009): Parasitic Infections of Free Range Chickens from Golestan Province, Iran. J. Parasitol, 4: 10-14. FAO (2008): Food and Agriculture Organization of the United Nations; Rome. Poultry sector country review. http://www.fao-ectad nairobi.org/IMG/pdf/ Ethiopia Poultry Sector Review. FAO. (2008): Chicken genetic resources used in smallholder production systems and opportunities for their development, by P. Sorensen. FAO Smallholder Poultry Production Paper No. 5. Rome. 66 Firaol, T., Dagmawit, A., Askale, G., Solomon, S., Morka, D. and Waktole, T. (2014): Prevalence of ectoparasite infestation in chicken in and around Ambo Town, Ethiopia. J. Vet. Sci. Technology 5: 189. doi:10.4172/2157-7579.1000189. Fisseha, M. and Tadelle, D. (2010: Characterization of village chicken and egg marketing systems of Bure district, North-West Ethiopia. Fisseha, M. (2009). Studies on production and marketing systems of local chicken ecotypes in bure woreda, north-west Amhara. Pp. 23. Gast, R.K., Stephens, J.F., Foster, D.N. (1988): Effects of kanamycin administration to poultry on the interspecies transmission of drug-resistant Salmonella. Poult. Sci.67:699–706. Gebreab, F. (1995): Zoonotic diseases in Ethiopia. Ethiopian Society of Animal Production, Addis Ababa, Ethiopia. Geleta, T., Leta, S. and Bekana, E. (2013): Production performance of fayoumi chickens under intensive management condition of Adami Tulu research center. Int. J. Livestock Prod., 4: 172- 176. Getu, A. and Birhan, M. (2014): Chicken production systems, performance and associated constraints in north Gondar zone, Ethiopia. World J. Agric. Sci., 10: 25-33. Gondwe, T.N. and Wollny, C.B. A. (2005): Evaluation of growth potential of local chickens in Malawi. Inter. Jour. of Poult. Sci., 4: 64-70. Guan, J., Chan, M., Grenier, C., Wilkie, D.C., Brooks, B.W., Spencer, J.L. (2009): Survival of avian influenza and Newcastle disease viruses in compost and at ambient temperatures based on virus isolation and real-time reverse transcriptase PCR. Avian Dis.53(1):26-33. Guèye, E. F. (2000): Women and family poultry production in Africa. Development in Practice 10: 98–102. 67 Hadipour, M.M., Habibi, G.H., Golchin, P., Hadipourfard, M.R., Shayanpour, N. (2011): The Role of Avian Influenza, Newcastle Disease and Infectious Bronchitis Viruses during the Respiratory Disease Outbreak in Commercial Broiler Farms of Iran. International J. Anim. Vet. Advances 3(2):69-72. Haftu, K. (2012): Effect of Replacing Maize with Malted Barley grain on Egg production, Quality, Fertility and Hatchability of White Leghorn layers. Msc Thesis, Haramaya University. Hagos, A. and Eshetu, Y. (2005): Ectoparasites of Local Scavenging Chickens of Central Ethiopia. Ethiop. J. Sci., 28(1):69–74. Hailemariam, M. (1998).Egg laying performance of White leghorn and their crosses with local birds at Debre Zeit, Ethiopia. Proceedings of 6th Conference of Ethiopian Society of Animal Production, May 14-15, 1998, Addis Ababa, Ethiopia, pp: 141-150. Haitham, M.Y. (2010): Effects of reducing dietary crude protein with amino acids supplementation on performance of commercial white leghorn layers during late production period. Egypt. Poult. Sci. 30(4): 975-988. Halima, H. (2007): Phenotypic and Genetic Characterization of indigenous chicken populations in northwest Ethiopia. PhD thesis, University of the Free State, Bloemfontein, South Africa. Hansen, J.W., Perry, B. (1994): The epidemiology, diagnosis and control of helminth parasites of ruminants. International Laboratory for Research on Animal Diseases, Nairobi, Kenya, pp. 171. Hassan, G.M., Farghaly,M., Soliman,F.N.K. and Hussain, H.A. (2000): The influence of strain and dietary protein level on egg production traits for different local chicken strains. Egypt Poult Sci. 20:49-63. Hirnik, J. F., J.D. Summers, J.P. Walker and Szkotnick, W. (1977): Production traits influencing the individual feed conversion ratio. Poultry Science. 56: 912-917. Hopla, C.E., Durden, L.A., Keirans, J.E. (1994): Ectoparasites and classification. Rev. Sci. Tech. 13: 985-1017. 68 Hossein, S., Shuhaimi, N.H. and Mehrafarin, R. (2012): The Settlement Patterns of Acheamenian Empire in Iranian’s Sistan (from 550 BC to 331 BC). In 4th Int. Cong. of Soci. of South Asian Archaeology (4SOSAA). Humphrey, T. J., Baskerville, A., Mawer, S., Rowe, B. and Hopper, S. (1989): Salmonella enteritidis phage type 4 from the contents of intact eggs: A study involving naturally infected eggs. Epidem. Inf. 103: 415–423. Hunton, P., (1995): Egg production, processing and marketing. In: World Poultry Science, Elsevier, Tokyo. Pp: 457-480. Idi, A., Permin, A., Jensen, S.K. and Murrell, K.D. (2007): Effect of minor vitamin A deficiency on the course of infection with Ascaridia galli (Schrank, 1788) and the resistance of chickens. Helminthologia 44 (1): 3-9. ILRI, (2015): African chicken genetic gains: Providing well-adapted chickens for African smallholders. ILRI Project Profile. Nairobi, Kenya. Javed, I., Nawaz, M., Khan, F.H. (2003): Pharmacokinetics and optimal dosage of kanamycin in domestic ruminant species. Vet. arhiv 73:323-331. Kansal, G., Singh, H.S. (2014): Incidence of Ectoparasites in Broiler Chicken in Meerut. IOSR- JAVS 7: 55-58. Khalil, L.F., Jones, A., Bray, R.A. (1994): Keys to the cestodes parasites of verterbrates. International Institute of Parasitology (an Institute of CAB International). Kinung’hi, S. M., Getachew, T., Hafez, M., Moges, W., Moses, K., Matthias G. and Maximillian, B. (2004): Assessment of Economic Impact Caused by Poultry Coccidiosis in Small and Large Scale Poultry Farms in Debre Zeit, Ethiopia Int. J. Poult. Sci., 3 (11):715-718. Krantz, G.W. (1978): A Manual of Acarology. 2nd Edition, Corvallis, Oregon, Oregon State University Book Store, pp. 508. 69 Kusina, J.F., Kusina, N.T., Mhlanga, J. (2000): Poultry production in Mashonal and Central Province: The role and opportunities for women. Integrated crop-livestock production in smallholder farming systems in Zimbabwe, proceedings of a review workshop, Harare, Zimbabwe. Pp: 247-264. Lapage, G. (1962): Moning Veterinary Helminthology and Entomology, 5th edition, Bailliere, Tindal and Cox, London, pp: 566-723. Lawal, A. M., Bello1, S. Y., Balami, Y., Wakil, Z. B., Yusuf, J., Dauda, E. S., Mshelia, H. P., Mana, M. K. and A. A. Biu. (2016): Prevalence and Economic Significance of Ectoparasites Infestation in Village Chickens (Gallus gallus domesticus) in Gombe, Northeastern Nigeria. Jour. of Agr. and Food Sci. 4 (5): 94-103. Lawrence, K.C., Windham, W.R., Park, B., Smith, D.P. and Poole, G.H. (2004): comparison between visible/NIR spectroscopy and hyper spectral imaging for detecting surface contaminants on poultry carcasses. Proceeding of the international society for optical engineering, 5271:35-52. Leeson, S. and Summers, J.D. (2001): The Nutrition of Chicken. 2nd ed. University Books, Guelph, Canada. Pp. 355. Leonard, D.K. (2000): The new institutional economics and the restructuring of animal health services in Africa. In: Leonard D.K., editor. Africa's Changing Market for Health and Veterinary Services. MacMillan Press Ltd; Basingstoke, UK. Leta, S. and Endalew, B. (2010): Survey on Village Based Chicken Production and Utilization System in Mid Rift Valley of Oromia, Ethiopia. Global Veterinaria, 5 (4):198-203. Mapiye, C., Mwale, M., Mupangwa, J., Chimonyo, M., Foti, R. and Mutenje, M. (2008): A Research Review of Village Chicken Production Constraints and Opportunities in Zimbabwe. Asian-Aust. J. Anim. Sci., 21 (11):1680-1688. McNabb, S.J., Hensel, D.M., Welch, D.F., Heijbel, H., McKee, G.L.and Istre, G.R. (1985): Comparison of sedimentation and flotation techniques for identification of Cryptosporidium sp. oocysts in a large outbreak of human diarrhea. Jour. of clin. mic., 22: 587-589. 70 Mekibib, B., Dejene, H. and Sheferaw, D. (2014): Gastrointestinal Helminthes of scavenging chickens in outskirts of Hawassa, southern Eithiopia. Global veterianaria 12(4): 557-561. Mekonnen, G. (2007): Characterization of Smallholder poultry production and Marketing system of Dale wonsho and Loka abaya weredas of southern Ethiopia. Msc. Thesis, Hawassa University. Mengesha, M., Tamir, B. and Dessie, T. (2011): Village chicken constraints and traditional management practices in Jamma District, south Wollo, Ethiopia. Lives. Res.for Rural Dev., 23 (37). Mesert, G. (2006): Effect of feeding peanut seed cake and brewery dried yeast on egg production, fertility and hatchability of Rhode Island Red chicken. M Sc Thesis, Alemaya University, Ethiopia. Pp: 52. Moges, F., Abera, M. and Tadelle, D. (2010): Assessment of village chicken production system and evaluation of the productive and reproductive performance of local chicken ecotype in Bure district, North West Ethiopia. African J. Agri. Res., 5 (13):1739-1748. Moges, F., Mellesse, A. and Dessie, T. (2010): Assessment of village chicken production system and evaluation of the productive and reproductive performance of local chicken ecotype in Bure district, North West Ethiopia. Afr. J. of Agric. Res. 5(13): 1739-1748. Mohammed, M.D., Abdalsalam, Y.I., Kheir, A.M., Jinyu, W. and Hussein, M.H. (2005): Comparison of the Egg Characteristics of Different Sudanese Indigenous chicken types. Int. J. of Poult. Sci., 4: 455- 457. Moyo, B. and Masika, P. J. (2015): Tick control methods used resource-limited farmers and the effect of ticks on cattle in rural areas of the Eastern Cape Province, South Africa. Trop. of Ani. Health and Prod. 41: 517-523. Muchadeyi, F., Wollny, C., Eding, H., Weigend, S., Makuza, M. and Simianer, H. (2007): Variation in village chicken production systems among agro-ecological zones of Zimbabwe. Trop. Anim. Health and Prod., 39:453-461. 71 Mukaratirwa, S., Hove T. (2009): A survey of ectoparasites, cestodes and management of free- range indigenous chickens in rural Zimbabwe. Tydskr. S. Afr. vet. Ver. 80(3): 188–191. Mulugeta, A., Chanie, M., Bogale, B. (2013): Major constraints of village poultry production in Demba gofa District of southern region, Ethiopia. British Jour. Poult. Sci. 2(1):1-6. Mwale, M. and Masika, J.P. (2011): Point prevalence study of gastro-intestinal parasites in village chickens of Centane district, South Africa. Afr. Jour. of Agr. Res., 6(8): 2033-2038. Nasser, M. (1998): Oral Newcastle Disease VaccinationTrial and Studies of NCD in Ethiopia. Msc. Thesis, Joint degree program, Addis Ababa University, Addis Ababa, Ethiopia, and Freie Universitat, Berlin, Germany,. Nigussie, D., Alemu, Y., Tadelle, D. and Samuel, W. (2003): On-station and on-farm evaluation of the ‘hay-Box chick brooder’ using different insulation materials at Debre Zeit Agricultural Research Center and Denbi village, Adaa woreda. In: Proceedings of the 10th annual conference of the Ethiopian Society of Animal Production (ESAP), August 21–23, held in Addis Ababa, Ethiopia. pp. 211–216. Njagi, L.W., Nyaga, P.N., Bebora, L.C., Mbuthia, P.G. and Minga, U.M. (2012): Effect of immunosuppression on newcastle disease virus persistence in ducks with different immune status. ISRN Veterinary Science, Volume 2012 (2012), Article ID 253809, 6 pages; doi: 10.5402/2012/253809. http://www.isrn.com/journals/vs/2012/253809 date 24/01/2012. Njue, S.W., Kasiiti, J.L., Macharia, J.M., Gacheru, S.G. and Mbugwa, H.C.W. (2001): A survey of the disease status of village chicken in Kenya. In: Livestock community and Environment. Proceedings of the 10th conference of the Association of Institutions for Tropical Veterinary Medicine, Denmark. Nnadozie, V.O. (1996): Prevalence of Ectoparasites of Local Chicken in Nsukka Area of Enugu State, Nigeria. Nsukka, Nigeria: University of Nigeria. 72 Nzietchueng, S. (2008): Characterization of poultry production systems and potential pathways for the introduction of highly pathogenic avian influenza in Ethiopia. Draft Report. International Livestock Research Institute. Ogbaje, C.I., Agbo, E.O. and Ajanusi, O.J. (2012): Prevalence of Ascaridia galli, Heterakis gallinarum and Tapeworm Infections in Birds Slaughtered in Makurdi Township. Inter J Poult Sci, 11(2): 103-107. OIE (2012): Newcastle disease. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 2.3.14.http://www.oie.int/ international-standard-setting/terrestrial-manual/ access- online. OIE. (2007): The Global Strategy for Prevention and Control of H5N1 Highly Pathogenic Avian Influenza. Rome: Food and Agriculture Organization of the United Nations. Okeno, T.O., Kahi, A.K. and Peters, J.K. (2011): Characterization of indigenous chicken production systems in Kenya: Household flock structures, dynamics and breeding practices. Tropical Animal Health and Production, DOI: 10.1007/s11250-011-9942-x. Okuthe, S.O. (1999): Participatory epidemiology assessment of livestock production constraints in the western Kenya highlands. PhD Thesis, University of Reading, United Kingdom. Oljira, D., Melaku, A., Bogale, B. (2012): Prevalence and risk factors of coccidiosis in poultry farms in and around Ambo Town, western Ethiopia. The American-Eurasian J. of Sc. Res. 7(4):146–149. Olwande, P.O., Ogara, W.O., Okuthe, S.O., Muchemi, G., Okoth, E., Odindo, M.O. and. Adhiambo, R.F., (2010): Assessing the productivity of indigenous chickens in an extensive management system in southern Nyanza, Kenya. Tropical Animal Health and Production, 42: 283- 388. DOI10.007/s11250-009-9418-4. Olwande, P.O., Ogara, W.O., Okuthe, S.O., Muchemi, G., Okoth, E., Odindo, M.O. and Adhiambo, R.F. (2014): Assessing the productivity of indigenous chickens in an extensive 73 management system in southern Nyanza, Kenya. Tropical Animal Health and Production, 42: 283- 388. Ondwasy, H., Wesonga, H. and Okitoi, L.O. (2006): Indigenous chicken production manual. In: KARI Technical Note No. 18, February 2006. Onwudike, O.C. (1981): Effect of various protein sources on egg production in tropical environment. Trop. Animal Prod. 6: 249-256. Opara, M.N., Osuji, C.T. and Opara, J.A. (2010): Gastrointestinal parasitism in Captive Animals at the Zoological Garden, Nekede Owerri, Southeast Nigeria. Report and Opinion 2: 5. Pagani, P., Wossene, A. Rome; Consultative Mission, Food and Agriculture Organization of the United Nations: 2008. Review of the new features of the Ethiopian poultry sector biosecurity implications. Payne, W. and Wilson, R., (1999): An introduction to Animal Husbandry in the tropics. Blackwell Science Ltd, Pp: 447-484. Permin, A. (2008). Good practices in small scale poultry production: a manual for trainers and producers in east Africa. A consultancy report to FAO, Addis Ababa, Ethiopia. Permin, A. and Hansen, J.W. (1998): The Epidemiology, Diagnosis and Control of Poultry Parasites. Food and Agriculture Organization of the United Nations, Rome, Italy, pp. 29-30. ISBN 92-5-104215-2. Pestka, D., Stenzel, T., Koncicki, A. (2014): Occurrence, characteristics and control of pigeon paramyxovirus type 1 in pigeons. Pol. J. Vet. Sci. 17(2):379-84. Rahman, A. W., Salim, H. and Ghause, M. S. (2009): Helminthic parasites of scavenging chickens (Gallus domesticus) from villages in penang Island, Malaysia. Tropical Life Science Research, 20(1):1-6. 74 Rahman, M., Sorensen, P., Jensen, H. and Dolberg, F. (1997): Exotic hens under semiscavenging condition in Bangladesh. Liv. Res. For rural Dev., 9:3. Rashid, M. M., Islam, M. N., Roy, B. C., Jakobsen, K. and Lauridsen, C. (2004): Effect of dietary supplementation of energy and protein on production performance and egg quality of scavenging crossbred hens in rural areas under tropical conditions. Livestock Research for Rural Development, 16: 63. Reta, D. (2009): Understanding the role of indigenous chickens during the long walk to food security in Ethiopia. Livestock Research for Rural Development, 21 (8): 116. Saidu L., Abdu P.A., Umoh J.U., Abdullahi S.U. (1994).Diseases of indigenous chickens. Bulletin of Animal Health and Production in Africa. 42:19–23 Senayt, A. (2011): The Effect of Feeding Different Levels of Soybean mealon egg production, quality, fertility and hatchablity of white leghorn layers. Msc thesis, Haramaya University. Shanta, I.S., Begum, N., Anisuzzaman, B.A.S.M., Karim, M.J. (2006): Prevalence and clinico- pathological effects of ectoparasites in Backyard poultry. Bangl. Jour. Vet. Med. 4: 19-26. Sharma, S., Azmi, S., Iqbal, A., Nasirudullah, N. and Mushtaq, I. (2013): Pathomorphological alterations associated with chicken coccidiosis in Jammu division of India. Jour. Parasitic Dis, doi: 10.1007/s12639- 013-0302-9. Shirzad, M. R., Seifi, S., Gheisari, H.R., Hachesoo, B.A., Habibi, H. andBujmehrani, H. (2011): Prevalence and risk factors for subclinical coccidiosis in broiler chicken farms in Mazandaran province, Iran. Trop. Anim. Health Prod. 43:1601-04. Shivaprasad, H. L. (1997): Pullorum Disease and Fowl Typhoid in Disease of poultry by Calnek, B.W., John, H., Barnes, C. W., Beard, L., McDougald, R., Saif, Y. M. 10th edition, Iowa State University Press, Iowa, USA, pp. 82-93. Smith, A.J. (2001): The tropical agriculturist. Macmillan publishers Ltd. London, pp 2 – 7. 75 Snoeyenbos, G. H. (1991):Pullorum disease. In Diseases ofPoultry, 9th ed., pp. 73–86. Edited by B. W. Calnek. Ames, IA: University of Iowa Press. Solomon, D. (2007): Suitability of hay-box brooding technology to rural household poultry production system. Lives. Res. Rural Dev., 19 (1): 21. Solomon, D. (2007): Suitability of hay-box brooding technology to rural household poultry production system. Lives. Res. Rural Dev., 19 (1): 63-64. Solomon, D. (2007): Suitability of hay-box brooding technology to rural household poultry production system. Lives. Res. Rural Dev., 19: 71-74. Solomon, D. (2008): An Analysis of poultry sector in Ethiopia. Food and agricultural organization, animal health and production, Viale delle Terme di Caracalla, 00153 Rome, Italy. Solomon, M. and Elsabet, G. (2010): Prevalence of External parasite of poultry in intensive and backyard chicken farm at Wolayta Soddo town, Southern Ethiopia. Veterinary World. 3(12): 533- 538. Sonaiya, E. B. (1990): The context and prospects for development of smallholder rural poultry production in Africa. CTA-Seminar proceedings on smallholder rural poultry production 9-13 October 1990, Thessaloniki, Greece, 1: 35-52. Soulsby, E.J.L. (1982): Helminths, Arthropod and Protozoa of Domesticated Animals, 7th edition, Bailliere Tindal, London, pp: 476- 486. Swai, E.S., Karimuribo, E.D., Kyakaisho, P.F. and Mtui, P.F. (2007): Free-range village chickens on the humid coastal belt of Tanga, Tanzania: Their roles, husbandry and health status. Livestock Research for Rural Development, 19 (8): 9. Tadelle, D. (1996): Studies on village poultry production systems in the central highlands of Ethiopia. MSc. Thesis, Swedish University of Agricultural Sciences, Uppsala, Sweden. 76 Tadelle, D. (1997): The Role of Scavenging Poultry in Integrated Farming Systems in Ethiopia. Debre Zeit Agricultural Research Centre. Tadelle, D. (2001): The role of scavenging poultry in integrated farming systems in Ethiopia. Debre Zeit Agricultural Research Center, Debre Zeit, Ethiopia. Livestock feed resources within 55 integrated farming systems. Pp: 377–399. Tadelle, D. (2010): Reducing the impact of infectious diseases on village poultry production in Ethiopia. Project Summaries in Investors of Department for International Development. Tadelle, D. and Ogle B. (2001): Village poultry production systems in the central highlands of Ethiopia. Tropical Animal Health and Production. 33(6), 521-537. Tadelle, D. and Ogle, B. (1996): Effect of maize (Zea mays) and noug (Guizotia abyssinica) cake supplementation on egg production performance of local birds under scavenging conditions in the central highlands of Ethiopia. MSc Thesis, Swedish University of Agricultural Sciences, pp. 14. Tadelle, D. and Ogle, B. (2000): Nutritional status of village poultry in the central high lands of Ethiopia as assessed by analyses of crop contents. Eth. J. Agri. Sci., 17:47-57. Tadelle, D., Million, T., Alemu Y. and Peters, K. J. (2003): Village chicken production system in Ethiopia. Flock characteristics and performances, Lives. Res. for Rural Dev. 15(1): Pp 4 – 8. Tadelle, D., Million, T., Allemu, Y. and Peters, K.J. (2003): Village chicken production systems in Ethiopia: 1. Flock characterization and performance. Liv. Res. for Rural Dev., 15 (1): 2003. Tadesse, D. (2010): Management Practices, Productive performances and egg quality traits of Exotic chickens under village production system in East Shewa, Ethiopia. Msc Thesis, Addis Ababa University in Tropical Animal Production and Health. Pp.48. Tadesse, S., Aschenafi, H., Aschalew, Z. (2008): Seroprevalence study of Newcastle disease in local chickens in central Ethiopia. Int. J. Appl. Res. Vet. Med. 3: 25–29. 77 Takele, T. and Oli, W. (2011): Uses and flock management practices of scavenging chickens in Wolaita Zone of southern Ethiopia. Trop. Anim. Health Prod., 44:537-544. Tesfaheywet, Z. and Yonas, Y. (2015): Ectoparasite infestation of free scavenging chickens reared under traditional backyard production system in Wolayita Zone, southern Ethiopia. Ethiop. Jour. vet. Med., 19 (2): 55-66. Tesfau, T. (2007). Chicken production systems and monitoring around the villages of diredawa town. M. Sc. Thesis Presented to School of Graduate Studies of HaramayaUniversity, Ethiopia. Pp. 47-49. Teshome, M. (1993): Preliminary survey of gastro-intestinal helminthes in local chickens in and around Sodo. DVM thesis, Faculty of Veterinary Medicine, Addis Ababa University, Ethiopia. Thrusfield, M. (1995): Veterinary epidemiology. 2nd ed., Blackwell Science, Oxford, England, pp. 479. Upton, M. (1979): Farm Management in Africa: the principle of production and planning. Oxford University press, Great Britain. Pp: 282-298. Urquart, G.M., Armour, J., Duncea, J.L., Dunn, A.M. and Jennigns, F.W. (1987): Veterinary Parasitology. Churchill Living stone Inc, New York. Pp: 8–170. Urquhart, G.M., Armour, J., Duncan, J.L., Dunn, A.M. and Jennings, F.W. (1996): Veterinary Parasitology. 2nd ed. Blackwell Science Lt., UK. USAID (United States Agency for International Development); 2010. Partnership for safe poultry in Kenya (PSPK) Program: Value chain analysis of poultry in Ethiopia. http://pdf. usaid.gov/pdf docs/ PNADU 076. Villamor, E. and Fawzi, W.W. (2005): Effects of vitamin A supplementation on immune responses and correlation with clinical outcomes. ClinicalMicrobiology Reviews 18 (3): 446–464. 78 Wachira, M.A., Mail, S.K., Munyasi, J.W., Nzioka, M., Mwangi, D.M., Kaguthi, P. and Kithome, J. (2010): Uptake of improved technologies through dissemination by indigenous chicken service providers in Southern Rangeland of Kenya. In: Proceedings at the 12th KARI Biannual Scientific Conference in November 2010, KARI headquarters, Kaptagat road off Loresho, Nairobi. Pp: 1376- 1382. Waldroup, W. P. (2002): Soybean meal in poultry nutrition. Poultry science, 0.210 faretteville AR 72701.Arakansas.www.soymeal.org. Walker, T.R. and Farrel, D.J. (1976): Energy and nitrogen metabolism of diseased chickens: Inter- action of Ascaridia galli infestation and vitamin A status. British Poultry Science 17: 63-77. Wilson, R. (2010): Poultry production and performance in the Federal Democratic Republic of Ethiopia. World's Poult. Sci. J., Pp: 66. Wu, G., M.M. Bryant, R.A. Voitle, and D.A. Roland Sr. (2005): Effect of dietary energy on performance and egg composition of Bovans White and Dekalb White hens during phase 1. Poult Sci. 84:1610–1615. Xiohe, (2010): Feed the impact of fiber on poultry production. http://www. articlefield.com/ 46836/feed. The-impact-of-fiber-on-poultry. Yakubu, A. (2010): Indigenous chicken flocks of Nasarawa state, Nigeria: Their characteristics, husbandry and productivity. Tropical and Subtropical Agroecosystems 12: 69-76. Yami, A. and Desie, T. (1997): The status of poultry research and development in Ethiopia. Proceedings of the 5th National Conference of Ethiopian Society of Animal Production (ESAP), May 15-17, Addis Ababa, Ethiopia. Pp: 40-60. Yehualashet, B. (2011): A study on the prevalence of the helminth parasites in free range (backyard) chicken in selected small holder farms in and around Haramaya. DVM Thesis, Haramaya University, Ethiopia. Pp: 1-48 Zumani, B. (2011): Ectoparasites of indigenous Malawi chickens. Aust. J. Basic and Appl. Sci., 5(6): 1454-1460. 79