Centro lnternoclonot de Agricultura TroPICal lntemotlonal Center tor Tropical Agrlcutture COLLABORATIVE PROJECT · Between CIRAD-CA, CIAT, and FLAR RICE IMPROVEMENT: USING GENE POOLS AND POPULATIONS WITH RECESSIVE MALE-STERILE GENE, . . . AND CONVENT1 J . ~AL BREEDING . . . 1998 Report Marc Chátel, Yolima Ospina, and Jaime Horrero CIRAD The Centre de coopération intemationale en recherche agronornique pour le développement (CIRAD) is a French research organization that specializes in agriculture in the tropics and subtropics. It is a state-owned body and was established in 1984, following the consolidation of French agricultura!, veterinary, forestry, and food technology research organizations for the tropics and subtropics. CIRAD's mission is to contribute to the econornic development of these regions through research, experiments, training, and dissemination of scientific and technical information. The Center employs 1800 persons, including 900 senior staff, who work in about 50 countries. Its budget amounts to approxirnately 1 billion French francs, more than half of which comes from public funds. CIRAD is made up of seven departments: CIRAD-CA (annual crops), CIRAD-CP (tree crops}, CIRAD-FLHOR (fruits and horticultura! crops), CIRAD-EMVT (livestock production and veterinary medicine), CIRAD-Foret (forestry), CIRAD-SAR '(food technology and rural systerns), and CIRAD-GERDAT (management, common services and laboratories, documentation). CIRAD operates through its own research centers, national agricultura! research systems, or development projects. CIAT The International Center for Tropical Agriculture (CIA T, its Spanish acronym) is dedicated to the alleviation of hung~r. and poverty in developing countries. CIA T applies science to a~inc!.eas.~ food production while sustammg the natural resource base. ~dJr:~.: - .-~· .r7'' ; -.. ·~, ~ . . . . . ~ . CIA T is one of 16 intemational agricultura! research centers sponsored ~y the-Con5ultative Group on Intemational Agricultura! Research ( CGIAR). ' · ·- u:-: i u1 0 í:. . .-. _ . " The Center's core budget is fmanced by 25 donor countries, intemational an(:lre.gi~~al d~elopmtmtioYganizations, and prívate foundations. In 1997, the donor countries include Australia, ~H~I'tJáUJ.O~anada, Colombia, Denmark, France, Germany, Japan, the Netherlands, Norway, Spain, Sweden, Switzerland, the United Kingdom, and the United States of America. Donor organizations include the European Union (EU), the Ford Foundation, the Inter- American Development Bank, the International Development Research Center (IDRC), the International Fund for Agricultura! Development (IFAD}, the Nippon Foundation, the Rockefeller Foundation, the United Nations Development Program (UNDP}, and the World Bank. Information and conclusions reported in this document do not necessarily reflect the position of any donor agency. FLAR The Fund for Latin American and Caribbean Irrigated Rice (FLAR} is a means by which the public and prívate sectors of Latin American and Caribbean (LAC) countries can control and take responsibility for irrigated rice activities. FLAR began in January 1995, after an Act of Acceptance was signed by delegates from Brazil, Colombia, Venezuela, the Intemational Center for Tropical Agriculture (CIA T), and the Intemational Rice Research Institute (IRRI). In 1996, Costa Rica, Panama, and the Centre de coopération internationale en recherche agronomique pour le développement (CIRAD) became members. FLAR's rnission is to promote sustainable development of irrigated rice production in LAC, that is, to make it competitive, profitable, and efficient while lowering relative prices ofrice for the consumer. FLAR's objectives are: • To provide up-to-date information on market needs and opportunities of member countries through a permanent forum. • To pursue a broad approach in regional rice actívities that are of interest to all members. • Increase sustainable rice production, that is, ensure the efficiency of production, equitable distnbution of benefits, and resource conservation. • To focus mainly on irrigated rice. '.> ~¿q 19/ i.:.r 1 t/ 3 ... ¡c¡c¡~ CONTENTS SUMMARY Collaborative Project between CIRAD, CIAT, and FLAR. Proyecto Colaborativo entre CIRAD, CIAT, y FLAR. CHAPTER l. n. m. IV. TABLES Presentation Recurrent Selection for Upland Savanna Rice Conventional Breeding for Upland Savanna Rice Recurrent Selection for Lowland Rice APPENDIX l. CIAT rice project IP-4 Individual work plan for 1998 Germplasm Registration 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. Publications, Conferences, and Posters Training activities References Reciproca} Visits from Scientists Visits from LAC Scientists Trips Special services for NARDS Countries maps COLLABORATIVE PROJECT BETWEEN CIRAO, CIAT, ANO FLAR Rice lmprovement, Using Gene Pools and Populations with Recessive Male-Sterile Gene And Conventional Breeding Marc Chátel, Yolima Ospina, and Jaime Borrero 1998 Report: Summary 1. BACKGROUND Genetic uniformity, or lack of genetic diversity, is of major concern to breeders, geneticists, and the agricultura! community in general. In many crops, genetic improvement is usually accomplished by reducing genetic diversity in the gene pools used to develop new varieties. But genetic uniformity is now considered as increasing a crop's potential vulnerability to disasters caused by biotic or abiotic constraints. One way of broadening the genetic base of Latín American rice and assessing the genotype-by- environment interaction is to identify specific potential parents and pool them to develop new, genetically broad-based, breeding material. CIAT and CIRAD's new breeding strategies focus on developing and improving populations to provide sources of potential parents with specific traits required by national breeding programs. One suitable breeding method to achieve this goal is recurrent selection. 2. RECURRENT SELECTION FOR UPLANO RICE IN THE SAVANNAS ANO HIGHLANOS 2.1. lntroduction The upland rice recurrent selection project aims to adapt, develop, and select upland rice gene pools and populations. The main characteristics of germplasm for savanna conditions are: Tolerance of soil acidity Resistance to diseases, mainly rice blast (Pyricularia grizea Sacc.) Resistance to pests, mainly rice plant hopper (Tagosodes orizicolus Muir.) Good grain quality (translucent, long, slender grain) Early maturity (total cycle about 115 days) For highland areas, we are looking for: Tolerance of cold temperatures Grain yield potential Grain quality 2.2. Upland Savanna Rice The activities reported here were conducted at Palmira Experiment Station (PES) and at "La Libertad" Experiment Station (LES).\ 2.2.1. Line development from recurrent populations: Generation 52 Populations PCT-5\PHB\1\0,PHB\1; PCT-A\PHB\1\0, PHB\1; and PCT -4\PHB\1\1,PHB\1 From the 69 lines evaluated at LES, 8 were selected (11 .6%). From each selected line, we chose 6 individual fertile plants. The 48 S3 lines (8 families of 6 lines) will be grown at PES during 1998 B. 2.2.2. Line development from recurrent populations: Generation 54 Populations PCT-5\PHB\1\0, PCT-A\PHB\1\0, and PCT -4\PHB\1\1 From the 150 S41ines evaluated at LES, 35 were selected (23.3%). From each selected line, we chose 6 individual plants. The 21 O SS lines will be grown at PES during 1998 B. 2.2.3. Line development from recurrent populations: Generation 56 2.2.3.1. Populations PCT -5\0\0\0, PCT -A\0\0\0, and PCT -4\0\0\1 From the 282 S6 lines evaluated at LES, 64 were selected (22.7%). From each selected line, we chose 6 individual plants.The 384 S?lines (64 families of 6 lines) will be grown at PES during 1997 B. 2.2.3.2. Population PCT -4\0\0\1>52 From the 96 S6 lines evaluated at LES, 12 were selected (12.5%). In each selected line, we harvested 6 individual plants. The 72 S? lines (12 families of 6 lines) will be grown at PES during 1997 B. 2.2.3.3. Populations PCT -5\0\0\0, PCT -A\0\0\0, and PCT -4\0\0\1. Plant selectlon in 53 lines at PES, 1996 B From the 18 S6 lines evaluated at LES, 2 lines (11.1 %) were selected from the population PCT- 4\0\0\1 . From each selected line, we chose 6 individual plants. The 12 S? lines (3 families of 6 lines) will be grown at PES during 1997 B. 2.2.4. Line development from recurrent populations: Advanced generations 2.2.4.1. From populations with a male-sterile gene The best advanced lines were used to set-up the INGER LAC VIOAL acisd soil nursery, to be distributed to the regional NARDS .. 2.2.4.2. From populations with no male-sterile gene Four (4) lines showing very good adaptation to acid soil condition were evaluated and seed increased at LES. 2.3. Population Enhancement - Recurrent Selection The CIAT rice project emphasizes the enhancement of populations and is phasing out the production of fixed lines for direct release by the region's national programs (NARS). The strategy is to develop and enhance gene pools and populations for well-targeted trait(s) to be used as sources of potential parents by national breeding programs. In the first 2 years of the recurrent selection project, we concentrated on introducing germplasm from Brazil (EMBRAPA Arroz e Feijao [formerly CNPAF] and CIRAD) and French Guiana, characterizing and mass selecting it. From 1995 onward, we concentrated our activities on enhancing and developing new populations. 2.3.1. Recurrent selection based on 52 line evaluation: Population PCT -4\0\0\1 During 1997 A, the population PCT -4\SA\1\1 was grown at LES to go through a second selection cycle. 2.3.1.1. 5electing fertile plants, and 52 evaluation In 1997 A, a total of 155 SO plants were selected. A sample of each SO seed was kept in the cold chamber. The S1 generation was grown during 1997 B at PES, and S2 seeds harvested. The S2 lines were evaluated during 1998 A at LES. The set of S2 lines was remited to Brazil, Bolivia and Venezuela for evaluation and line development by the respective lnstitutions of these countries. 2.3.1.2. Harvesting male-sterile plants During 1997 A, male-sterile plants were harvested individually and their seeds mixed in equal proportions to complete the second cycle of recombination of the population selected once. The second cycle of recombination, identified as PCT -4\SA\2\1 was grown at LES during 1998 A. 2.3.2. Mass recurrent selection for both sexes for major agronomic traits, blast, and "hoja blanca": Populations, PCT-5\0\0; PCT-A\0\0\0; and PCT -4\0\0\1 During 1997 A, the seed mixture of each population with two mass recurrent selection cycles was grown at LES. To make the third recurrent selection cycle, 218, 253, and 165 healthy male- sterile plants, fertilized by the palien of fertile healthy plants, were selected from each population. In 1998 A, each population with three cycles of recurrence was grown at LES. Healthy male- sterile plants were selected ((180, 200, and 240, respectively). Fertile plants (30, 24, and 55, respectively) were selected for line development from the third cycle of recurrence. 2.4. Development of New Populations The development of new populations is a basic activity of the project. lt forms the main source of new recombined variability for population enhancement and line development. In 1996 B, we decided to build, at PES, two new Japonica populations, targeting upland savannas and hillside ecosystems. The source of male-sterility background is the best Japonica population previously developed by the project. 2.4.1. Upland savanna population The idea in developing that population is to pool the best lines of the CIA T conventional rice breeding project and the commercial varieties released in Brazil, Colombia, and Bolivia. In 1996 B, 18 lines were selected, based on their performance for early maturity, blast and acid- soil tolerance, and grain quality. Male-sterile plants from the best-adapted upland Japonica population (PCT -4) were used as female parents. During 1997 A, at PES, each resulting F1 was grown individually and evaluated, and individual plants selected. The basic population PCT-11\0\0\0 is the result of the mixture of the F2 seed. For the Savannas, the population PCT -11 was evaluated at LES, during 1998 A. 95 fertile plants So were selected for line development. The S1 generation will be grown at PES during 1998 B. 2.4.2. Upland hillside population The idea is to develop a population for the Andean highlands of Colombia, with early maturity and cold tolerance for high altitude, 1300-1600 meters above sea level. In 1996 8, 11 lines from Madagascar,'CIAT and IRAT were selected, based on their previous evaluation at high altitudes. The best-adapted upland Japonica population (PCT -4) was used as the source for male-sterility. During 1997 A, at PES, each resulting F1 was grown individually and evaluated, and individual plants chosen. The basic population PCT-13\0\0\0 is the result ofthe mixture of F2 seed. During 1997 8, at PES, the basic population will be recombined once. The identification of the first cycle of recombination of the basic population will be PCT -13\0\0\1 . During1998 A, at PES, the second cycle of recombination was made. The recombined population was then remitted to Dr. Michel Valés for further evaluation in the Colombian Andeas. 3. USE OF RECURRENT UPLAND GERMPLASM BY LAC PARTNERS 3.1. Bolivia. The populations PCT -4 and PCT-5 from CIAT and PCNA-16 from EMBRAPA, were characterized. Mass selection of male-sterile plants was made. Fertile So plants were selected in the population PCT -4 for line development. 3.2. Venezuela. The population PCT-11 was remitted to UNELLEZ and is under characterization 3.3. Cuba. In 1996, the populations PCT -4 was introduced and characterized fro different agronomic traits. In 1997, the germplasm was recombined one more time, and 19 So fertile plants selected for line development. The development of a local population is under way, by introducing 4 Cuban lines in the population PCT -4. 4. CONVENTIONAL BREEDING FOR UPLAND RICE FOR SAVANNAS ANO HIGHLANDS 4.1. Savannas Upland Rice 4.1.1. Upland lines from Brazil. We received from CNPAF a set of Brazilian lines for evaluation under acid-soil conditions 4.1.2. Use of CIAT/CIRAD savanna lines in Brazil On average, 89%, 28%, and 19% of the lines evaluated in the Advanced, Preliminary, and observation trials are CT lines .. The main characteristics they like from our lines are grain quality and plant type. 4.1.3. Line release in Brazil Three new CT lines are very promising candidates to be released in 1999. 4.1 .4. Une release in Bolivia The line named IRA T 170 ids to be released for small farmers. 4.1.5. Use of CT and IRA T lines in China The line IRA T 359 is to be released in the Yunnan Province of China. A great number of CT lines are used as promising parents by the Chinese upland breeding program. 4.2. Upland Rice for the Highlands In 1993, upland lines developed by CIRAD/FOFIFA for the highlands of Madagascar were introduced to Colombia and seed increased. The new germplasm was distributed to CENICAFE and CIA T's híllsides project. In 1994, line evaluation started in the Department of Ca u ca. In 1995, the Centro Internacional de Agricultura Orgánica (CIAO) started evaluations at 1600 masl. The first results were presented at the Conference on Rice for the Highlands in Madagascar in April 1996. 4.2.1. The Coffee Region - CENICAFE and CIAO Une CT 10069-27-3-1-4, well adapted to the mid-altitudes, was used in a trial with young coffee trees. The potential of this line over time is very promising (average grain yield is 4 t/ha). Forty- one new lines were introduced from Madagascar and seed increased at CIAT, Palmira. Eleven single crosses were made at PES, between line CT 1 0069-27-3-1-4 and 1 O lines from Madagascar and CIRAD. The F1 generation was grown during 1997 A at PES. Dr. Michel Vales arrives at CIAT in August 1997 and took the responsibility of the breeding activities for the Highlands of Latín America. For more information about the activities developed during 1998, please report to his annual report. 4.2.2. Department of Cauca and Central America In the Department of Cauca, the five best lines of the previous year's selection and one savanna upland check (CIRAD 409) were tested on farm by five smallholders. The best line (Latsidahy/FOFIFA 62-3) yielded 1400 kg/ha at La Laguna (1600 masl). The savanna upland check showed complete sterility. The 41 introduced lines were dispatched to the CIAT hillsides project for testing in Colombia and Central America. Dr. Michel Vales arrives at CIAT in August 1997 and took the responsibility of the breeding activities for the Highlands of Latín America. For more information about the activities developed during 1998, please report to his annual report. 5. RECURRENT SELECTION FOR LOWLAND RICE 5.1. lntroduction The recurrent selection breeding project was started by introducing different gene pools and populations developed in Brazil, by EMBRAPA Arroz e Feijao and CIRAD in French Guiana. The germplasm was characterized at CIAT, Palmira, and the best-adapted populations were used to develop new populations by incorporating new variability. This resulted in three populations that were registered in the recurrent selection catalogas PCT-6, PCT-7, and PCT-8. A gene pool was also built up by using a different gene for male sterility. The gene pool was registered as GPCT-9. Finally, a second gene pool from CIRAD was registered as GPIRAT-10. 5.2. Recurrent Selectlon: Recurrent Selection for Both Sexes for "Hoja Blanca" The objective was to use the recurrent selection breeding method for existing gerrnplasm for "hoja blanca" virus and blast resistance. Three populations, PCT-6\0\0\2, PCT-7\0\0\0, and PCT-8\0\0\0, and the gene pool GPCT-9\0\0\0F were evaluated for "hoja blanca" virus according to the methodology developed at CIAT. Healthy plants of each gerrnplasm were transplanted separately for recombination with male-sterile plants. The recombined populations, after the first cycle of selection, were identified as PCT-6\HB\1\2, PCT-7\HB\1\0, PCT-8\HB\1\0, and GPCT- 9\HB\1\0F. During 1998, two more recurrent cycles were perforrned at PES for PCT-7 and PCT-8. The population PCT-6 better adapted to the tropics was remitted to Dr. Michel Valés. The enhanced population is the starting point of his recurrent selection project for partial blast resistance. 5.3. Recurrent Selection in Colombia In 1996, Dr. Hemando Delgado from CORPOICA attended the lntemational Course on Rice Recurrent Selection Breeding held at CIAT and selected four germplasm materials (populations PCT -6\0\0\2, PCT-7\0\0\0, and PCT-8\0\0\0, and the gene pool GPCT-9\0\0\0F). Each material was grown separately at LES for recombination, characterization, and selection of fertile plants for line development. The four materials performed well. The populations PCT-6 and PCT-7 presentad the highest potential for the future. The two populations were grown at Villavicencio and evaluated for blast resistance. 5.4. Recurrent Selection in Costa Rica In 1996, we sent Costa Rica the Indica gene pool GPCT-9 and population PCT-7. That same year, Dr. Randolph C. Morera, of the National Rice Program, attended the lntemational Course on Rice Recurrent Selection Breeding held at CIAT. The gerrnplasm was characterized under Costa Rican conditions and was maintained by harvesting male-sterile and fertile plants, independently. In 1997, the gerrnplasm was used for line development by selecting SO fertile plants. For 1998, the results are not yet available 5.5. Recurrent Selection in El Salvador In 1995, we sent three populations (PCT-6, PCT-7, and PCT-8) and the gene pool GPCT-9 to the Centro Nacional de Tecnología Agropecuaria y Floresta! (CENTA), where the following activities were carried out: • Une development: in 1996, 141 and 97 SO fertile plants were selected from the populations PCT-7 and PCT-8, respectively. • Population enhancement: based on S2 progeny evaluation and recombination from the remnant SO seeds. • New population development: the population CNA IRAT ES 1/0/2 was developed by introduction tour lines (X-1 O, CENTA A-1 , CENTA A-2, and CENTA A-5) into the Brazilian population CNA IRA T 4/0/6 For 1998, the results are not yet available 5.6. Recurrent Selection In Panama In 1996, we sent Panama the Indica gene pool GPCT-9 and population PCT-7. That same year, Dr. Ariel E. Jaén Sánchez of the Faculty of Agricultura! Sciences, Universidad de Panama, attended the lntemational Course on Rice Recurrent Selection Breeding held at CIAT. The introduced germplasm was grown and characterized. For each material, the earliest SO fertile plants were harvested for line development. For 1998, the results are not yet available 5.7. Recurrent Selectlon in Venezuela After he attended the lntemational Course on Rice Recurrent Selection Breeding, three populations (PCT -6, PCT-7, and PCT-8) and three gene pools (IRAT 1/420P, IRAT MANA, and GPCT-9) were sent to Dr. E. Graterol for characterization under the local conditions of Calabozo, Guárico State. The objective was to select the best-adapted germplasm to start a recurrent selection program. Two populations, PCT-6 and PCT-7, were selected as male-sterile background to develop two new local populations, identified as PFD-1 and PFD-2. During 1998, at DANAC-Venezuela, and at CIAT Palnira, the built-up of the populations is on- going as planed. 5.8. Recurrent Selectlon in Cuba In 1996, the gene pool GPIRAT-10 and the population PCT-7 were introduced to Cuba, and characterized. The germplasm was seed increased through recombination. Fertile So plants were selected for line development. 5.9. Recurrent Selection in Argentina In December 1996, we supplied the Universidad de Corrientes with the populations PCT-6\0\0\0, PCT-7\0\0\0, and PCT-8\0\0\0. The germplasm was observed and characterized. A total of 171 lines was selected from the three populations. The populations were recombined and seed increased. The Chilean population PQUI-1 was remitted to the Universidad de la Plata, for evaluation in the Pampas region of the province of Buenos Aires. Development of new local populations: PARG-1, is a population with narrow genetic base for short term breeding. PARG-2, is a population with oriented variability from PARG-1 introduced into PCT-8 (the best introduced germplasm) PARG-3, is a population coming from the introduction of 6 Argentinean lines into PCT- 8. This work will be done at CIAT Palmira. 5.10. Recurrent Selection in Chile In 1996, we sent Chile the Japonica gene pool GPIRAT-10, developed by CIRAD specifically for temperate climates. That same year, Dr. Santiago Hernaiz from INIA-Quilamapú attended the lnternational Course on Rice Recurrent .Selection Breeding held at CIAT. , In 1997, the gene pool was grown to characterize and select fertile plants for line development. lt was also used as a source of male sterility to build up a local population by crossing five Chilean lines (Qui. 67108, Diamante, Buli, CINIA 609, and CINIA 606) with male-sterile plants of the gene pool. Part of the hybrid seed was sent to CIAT, Palmira, for growing the F1 generation. F2 seed was shipped back to Chile. The basic Chilean population was identified as PQUI-1\0\0\0. In 1998, the Chilean population was split in two part. The first one was grown at Chillan, and the second one Northern. These two sites correspond to two different agroclimatic situations. After recombination, the two populations were shipped to CIAT Palmira for the second recombination. 5.11. Recurrent Selection in Uruguay In 1996, we sent Chile the Japonica gene pool GPIRAT-10. That same year, Dr. Fernando Pérez de Vida attended the lntemational Course on Rice Recurrent Selection Breeding held at CIAT. In 1997, the gene pool was grown to characterize and select fertile plants for line development. lt was also used as a source of male sterility to build up a local population by crossing selected Uruguayan lines with male-sterile plants of GPIRAT-10. In 1998, at CIAT, we received the F1 's seeds from the different crosses, for seed increase (F1 generation and harvest of the F2's). Three populations will be set-up: PURG-1 from the introduction of 171ines with short grain into GPIRAT-10, PURG-2 from the introduction of 60 lines with long grain quality into GPIRAT-10, and PURG-3 with broad genetic base resulting of the mixture of the two previous populations, with the best selected progenies selected from GPIRAT-10. 5.12. Une Development through Anther Culture In 1994, we introduced from French Guyana, the population IRAT-CT. This population comes from the enhancement of the Indica gene pool GPCNA-18 for anther culture response. From 1995, the CIAT anther .culture laboratory processed the population IRA T-CT, and R2 lines were developed. The lines were evaluated by FLAR at the Santa Rosa Experiment Station and five lines were selected. During 1998, the five lines were evaluated and seed increased for future muftí local evaluation. 5.13. Conventional breeding and anther culture for Romania In the framework of the collaboration between the CIRAD's rice project in Montpellier and the Romanian lnstitution FUNDULEA, two crosses from Romania are being processed by the CIA T's anther culture laboratory. Double Haploids lines will be remitted to FUNDULEA, and we will keep sorne seeds for shipping to Chile, Argentina and Uruguay. These lines can also be useful as donors for cold tolerance in the hillsides of Colombia. 1 PROYECTO COLABORATIVO ENTRE CIRAD, CIAT Y FLAR Mejoramiento en Arroz usando Acervos Genéticos y Poblaciones con un Gen Recesivo de Androesterilidad y Mejoramiento Convencional Marc Chatel, Yolima Ospina y Jaime Borrero Resumen 1998 1. ANTECEDENTES Uniformidad genética, o falta de diversidad genética, es la preocupación mayor de mejoradores, genetistas, y de la comunidad agrícola en general. En muchas cosechas el avance genético usualmente va acompañado por una reducción de variabilidad genética en los acervos utilizados para el desarrollo de nuevas variedades. Pero la uniformidad genética es ahora considerada como un potencial de incremento de vulnerabilidad a desastres bióticos y abióticos constrastantes. Una manera de ampliar la base genética del arroz en América latina y evaluando la interacción de genotipo por ambiente, es identificando padres específicos potenciales y acervos que desarrollen materiales de base genética amplia. El proyecto colaborativo entre el CIAT y el CIRAD, enfoca una estrategia en desarrollar y mejorar poblaciones que prevén fuentes de padres potenciales con características específicas requeridas por los programas nacionales. Un método satisfactorio para alcanzar esta meta, es la selección recurrente. 2. SELECCION RECURRENTE PARA ARROZ DE SECANO Y ZONA DE LADERAS 2.1 Introducción El proyecto de selección recurrente en arroz proyecta adaptar, desarrollar y seleccionar acervos y poblaciones de arroz. Las características principales del germoplasma para condiciones de sabanas son: . Tolerancia a la acidez del suelo . Resistencia a enfermedades, principalmente piricularia (Pyricularia grisea Sacc.) . Resistencia a plagas, principalmente Hoja blanca (Tagosodes orizico/us Muir.) . Calidad del grano Buena (grano translúcido, largo, delgado) . Precocidad (ciclo total aproximadamente de115 días) Para áreas de laderas: . Tolerancia a temperaturas frías . Potencial de rendimiento . Calidad del Grano 2.2. Arroz de sabanas Las actividades fueron conducidas en La Estación Experimental de palmira (EEP) y en "La Estación Experimental La Libertad" (EELL). 2.2.3. Desarrollo de Líneas de las poblaciones recurrentes, Generación 52, Poblaciones PCT-5\PHB\1\0, PHB\1; PCT-A\PHB\1\0, PHB\1 y PCT -4\PHB\1\1, PHB\1 De las 69 líneas evaluadas en la EELL, se seleccionaron 8 {11 .6%). De cada línea se escogieron 6 plantas individuales fértiles. Las 48 líneas S3 (8 familias de 61íneas) se sembrarán en la EEP durante 1998 B. 2.2.2. Desarrollo de Líneas de poblaciones recurrentes: Generación 54, Poblaciones PCT-5\ PHB\1\0; PCT-A\PHB\1\0; y PCT -4\PHB\1\1 Las 150 líneas S4, fueron evaluadas en la EELL y se seleccionaron 35 (23.3%). De cada línea seleccionada, se seleccionaron 6 plantas individuales. Las 210 líneas SS se sembraran en la EEP durant~ 1998 B. 2.2.3. Desarrollo de Líneas de poblaciones recurrentes: Generación 56: 2.2.3.1. Poblaciones PCT-5\0\0\0; PCT-A\0\0\0; y PCT -4\0\0\1 De las 282 líneas S6 evaluadas en la EELL, se seleccionaron 77 (27.3%). De cada linea se escogió 6 plantas individuales, para un total de 462 líneas S7 (77 familias de 6 líneas) El material se sembrará en la EEP en 1998 B. 2.2.3.2. Población PCT -4\0\0\1> 52 De las 96 líneas S6 evaluadas en la EELL, se seleccionaron 12 (12.5%). En cada línea se seleccionó 6 plantas individuales. Las 72 líneas S7 (12 familias de 6 líneas) se sembraran en la EEP durante 19988. 2.2.3.3. Poblaciones PCT -5\0\0\0; PCT -A\0\0\0; y PCT -4\0\0\1. Selección de plantas en líneas 53 (EEP 1996 B) De las 18 líneas S6 evaluadas en la EELL, 2 líneas (11 .1%) se seleccionó de la población PCT- 4\0\0\1 . De cada una se escogieron 6 plantas individuales. Las 12 líneas S7 (3 familias de 6 líneas) se sembrarán en la EEP, durante 1998 B. 2.2.4. Desarrollo de líneas de poblaciones recurrentes: Generaciones avanzadas 2.2.4.1. De poblaciones con un gen de androesterilidad Se utilizaron las mejores líneas avanzadas para el ensayo VIOAL, para suelos ácidos, deiiNGER LAC, distribuidas a los programas regionales. 2.2.4.2. De poblaciones sin gen de androesterilidad Cuatro líneas se seleccionaron por presentar buena adaptación a las condiciones de suelos ácidos, las cuales fueron multiplicadas en EEP. 2.3. Mejoramiento Poblacional a través de Selección Recurrente El proyecto colaborativo de Arroz del CIAT/CIRAD, hace énfasis al mejoramiento de poblaciones y extracción de líneas para los programas nacionales de la región. La estrategia es desarrollar acervos y poblaciones, con buenas características agronómicas, para ser utilizados como fuentes de progenitores potenciales y líneas fijas para los programas nacionales. En los primeros 2 años de la selección recurrente, el proyecto, se concentró en introducir germoplasma de Brasil (EMBRAPA Arroz e Feijao anteriormente CNPAF) y de la Guyana francesa (CIRAD), caracterizando y seleccionando masalmente. De 1995 en adelante, se concentraron las actividades en reforzar y desarrolla nuevas poblaciones. 2.3.1. Selección Recurrente basado en evaluación de líneas 52 de la Población PCT -4\0\0\1 Durante 1997A la población PCT -4\SA\1\1 se sembró en la EELL, iniciando con el segundo ciclo de la selección recurrente. 2.3.1 .1. Selección de plantas fértiles y evaluación de líneas 52 En 1997 A, un total de 155 plantas SO se seleccionaron y se guardó una muestra de cada semilla SO en el cuarto frío. Las líneas S1 se sembró durante 1997 B en la EEP para avance de generación y se cosechó semilla S2. Las líneas S2 se evaluaron durante 1998 en la EELL. El juego de líneas S2 fue remitido a Brasil, Bolivia y Venezuela para evaluación y desarrollo de líneas por las Instituciones respectivas de estos países. 2.3.1.2. Cosecha de plantas androesteriles Durante 1997 A, se cosecharon plantas androesteriles individuales y sus semillas se mezclaron en proporciones iguales, para completar el segundo ciclo de recombinación de la población, seleccionada una vez. El segundo ciclo de recombinación, se identificó como PCT -4\SA\2\ y se sembró en la EELL durante 1998 A. 2.3.2. Selección recurrente masal para ambos sexos para características agronómicas, piricularia y "hoja blanca": Poblaciones, PCT-5\0\0; PCT-A\0\0\0; y PCT -4\0\0\1 Durante 1997 A, se mezcló la semilla de cada población con dos ciclos de selección recurrente masal y se sembró en la EELL. El tercer ciclo de selección recurrente se obtuvo con, 218, 253, y 165 plantas androestériles fertil izadas por el polen de plantas fértiles sanas seleccionadas en cada población. En 1998 A , se sembró cada población con tres ciclos de recurrencia en la EELL. Se seleccionaron plantas androestériles sanas de las poblaciones PCT-5, PCT-A y PCT4 (180, 200, y 240, respectivamente) . Se seleccionaron también plantas fértiles (30, 24, y 55, respectivamente) para desarrollo de líneas a partir del tercer ciclo de recurrencia. 2.4. Desarrollo de Nuevas Poblaciones El desarrollo de nuevas poblaciones es una actividad básica del proyecto. Es la fuente principal de nueva variabilidad , recombinando y al mismo tiempo extrayendo material para desarrollo de líneas. En 1996 B se sintetizó, en la EEP, dos nuevas poblaciones Japónicas para secano y para el ecosistema de laderas. La fuente de androesterilidad fue obtenida a partir de la mejor población japónica previamente desarrolló por el proyecto. 2.4.1. Población para secano La idea de desarrollar esta población, fue el de agrupar las mejores líneas del proyecto de arroz del CIAT, obtenidas por mejoramiento convencional, y líneas que fueron lanzadas como variedades comerciales en Brasil, Colombia, y Bolivia. En 1996 B, 18 líneas se seleccionaron, por precocidad, enfermedades, tolerancia a suelos ácidos y calidad del grano. Las mejores plantas androestériles de la población PCT -4, fueron utilizadas como madres. Durante 1997A en la EEP, cada F1 resultante se sembró individualmente para evaluación y selección. La población básica PCT-11\0\0\0 es el resultado de la mezcla de la semilla F2. La población PCT-11 se sembró en la EELL durante 1998 A. 95 plantas fértiles se seleccionaron para desarrollo de líneas. La generación S 1, se sembró durante el segundo semestre de 1998 en la EEP durante 1998 B. 2.4.2. Población para Laderas El propósito fue el de desarrollar una población para la región Andina de Colombia, con precocidad y tolerancia al frío de altitudes altas, 1300-1600 msnm En 1996 B, 11 líneas de Madagascar, de CIAT e del CIRAD se seleccionaron, basada en su evaluación previa a altitudes altas. La población de secano PCT -4 mejor adaptada se utilizó como fuente de androesterilidad. Durante 1997 A , en la EEP, cada F1 resultante se sembró individualmente y evaluó, seleccionando plantas individuales. La población básica PCT-13\0\0\0 es el resultado de la mezcla de semilla F2. Durante 1997 B en la EEP, la población básica fue recombinada una vez. La identificación del primer ciclo de recombinación de la población básica es identificado como PCT -13\0\0\1. 1 Durante 1998 A en la EEP, se obtuvo el segundo ciclo de recombinación. Se remitió entonces a Dr. Michel Valés para evaluación más amplia en el ecosistema de laderas. 3. UTILIZACIÓN DE GERMOPLASMA DE SECANO POR LOS COLABORADORES DE DIFERENTES PAISES 3.1. Bolivia. Las poblaciones PCT -4 y PCT-5 de CIAT y PCNA-16 de EMBRAPA, se caracterizó. Se hizo selección de masa! de plantas androestériles fecundadas. Se seleccionaron plantas SO en la población PCT-4 para desarrollo de líneas. 3.2. Venezuela. La población PCT -11 fue remitida a UNELLEZ y está bajo caracterización 3.3. Cuba. En 1996, la población que se introdujo fue la PCT -4, la cual se caracterizó para diferentes características agronómicas. En 1997 el gerrnoplasma fue recombinado una vez mas y 19 plantas fértiles, fueron seleccionadas para desarrollo de lineas. El desarrollo de una población local esta en marcha, introduciendo 4 líneas cubanas en la población PCT -4. 4. MEJORAMIENTO CONVENCIONAL PARA ARROZ DE SECANO Y EL ECOSISTEMA DE LADERAS 4.1. Arroz de secano 4.1.1. Líneas de secano de Brasil. Recibimos de CNPAF un juego de líneas brasileñas para evaluación bajo condiciones de suelos ácidos. 4.1.2. Utilización de líneas de secano de CIAT/ CIRAD en Brasil. En promedio 89%, 28%, y 19% de las líneas evaluadas en el ensayo Avanzado Preliminar y ensayos de observación son líneas CT. Las características principales que gustan de nuestras líneas son calidad del grano y tipo de la planta. 4.1.3. Lanzamiento de Líneas en Brasil Tres líneas CT nuevas son candidatas muy prometedores para ser liberadas en el año de 1999. 4.1.4. lanzamiento de Línea en Bolivia La linea IRAT 170 esta siendo sembrada por pequeños agricultores. 4.1.5. Utilización de líneas CT e CIRAD en China La línea IRA T 359 está siendo sembrada en la provincia de Yunnan-China. Un gran número de líneas cr· son utilizadas como progenitores en su programa de mejoramiento. Una línea del GIRAD, la IRAT 359 es muy promisoria e debe ser lanzada en 1999. 4.2. Arroz de secano para laderas En 1993 líneas de secano desarrolladas por CIRAD/FOFIFA, fueron introducidas para el ecosistema de laderas en la región andina de Colombia, material que fue multiplicado en la EEP. Las líneas fueron distribuidas a CENICAFE y al proyecto de laderas del CIAT. En 1994, la evaluación de las líneas comenzó en el departamento del Cauca. En 1995 el Centro Internacional de Agricultura Orgánica (CIAO) comenzó evaluaciones a 1600 metros sobre el nivel del mar. Los primeros resultados fueron presentados en la Conferencia en Arroz para la región montañosa en Madagascar en abril de 1996. 4.2.1. La Región Cafetera- CENICAFE y CIAO La línea CT10069-27-3-1-4, se adaptó muy bien a altitudes medias, se utilizó en un ensayo con plantas de café jóvenes. El potencial de esta línea es muy prometedor (rendimiento del grano en promedio es de 4 t/ha). Se introdujeron 41 líneas nuevas de Madagascar y se multiplicó la semilla en CIAT, Palmira. Se realizaron 11 cruces simples en la EEP entre la línea CT1 0069-27- 3-1-4 y 10 líneas de Madagascar y CIRAD. La generación F1, fue sembrada durante 1997 en la EEP. El Dr. Michel Valés llegó a CIAT en agosto de 1997 y tomó la responsabilidad de las actividades del Mejoramiento para laderas en la región andina de América Latina. Para mayor información acerca de las actividades desarrolladas durante 1998, favor remitirse al informe anual. 4.2.2. Departamento del Cauca y Centro América En el departamento del Cauca, las mejores cinco líneas, obtenidas de la selección del año anterior, mas una línea de secano (CIRAD 409) utilizada como testigo, fueron probadas en una granjas por cinco propietarios de un minifundio. La mejor linea (Latsidahy/ FOFIFA 62-3) rindió 1400 kg/ha a una altitud de 1600 msnm. El testigo mostró completa esterilidad. Las 41 líneas introducidas de Madagascar, fueron enviadas al proyecto de laderas del CIAT para ser sembradas en Colombia y Centroamérica. Para mayor información, reportarse al informe anual. 5. SELECCION RECURRENTE PARA ARROZ RIEGO 5.1. Introducción EL mejoramiento por selección recurrente, inició con la introducción de diferentes acervos y poblaciones desarrolladas en Brasil, por EMBRAPA Arroz e Feijao y CIRAD en Guyana francesa. Se caracterizó el germoplasma en CIAT, Palmira y las poblaciones de mejor adaptabilidad, fueron utilizadas por el proyecto, para desarrollar nuevas poblaciones e introducir nueva variabilidad. De este estudio se obtuvieron tres poblaciones que se registraron en el catálogo de selección recurrente como PCT-6, PCT-7 y PCT-8. El acervo desarrollado sirvió como nueva fuente de androesterilidad. El acervo se registró como GPCT-9. Finalmente, el segundo acervo de CIRAD se registró como GPIRAT-10. 5.2. Selección Recurrente: Selección recurrente en Ambos Sexos para "Hoja Blanca" El objetivo era usar el método de mejoramiento de selección recurrente para el virus de "Hoja Blanca" y resistencia a enfermedades. Tres poblaciones, PCT-6\0\0\2, PCT-7\0\0\0, y PCT- 8\0\0\0, y el acervo genético GPCT-9\0\0\,0F, fueron evaluados para el virus de la hoja blanca , según la metodología propuesta por CIAT. Se trasplantaron separadamente por recombinación con plantas androestériles. La recombinación de las poblaciones, después del primer ciclo de selección, se identificó como PCT-6\HB\1\2, PCT-7\HB\1\0, PCT-8\HB\1\0, y GPCT-9\HB\1\0F. Durante 1998 se lograron dos ciclos recurrentes en la EEP para la PCT-7 y PCT-8. La población PCT-6, la que mejor se ajustó a los trópicos y se remitió a Dr. Michel Valés. La población mejorada para Hoja Blanca dará inicio a la selección recurrente para resistencia parcial a piricularia. 5.3. Selección Recurrente en Colombia En 1996, el Dr. Hernando Delgado de CORPOICA asistió al Curso Internacional en Arroz de Mejoramiento de Selección Recurrente, realizado en CIAT y seleccionó cuatro poblaciones (PCT- 6\0\0\2, PCT -7\0\0\0, y PCT -8\0\0\0, y el acervo genético GPCT -9\0\0\0F). El material fue sembrado separadamente en la EELL para recombinación, caracterización y selección de plantas fértiles para desarrollo de líneas. Los cuatro materiales se comportaron bien. Las poblaciones PCT-6 y PCT-7 presentó el potencial más alto para el futuro. En 1998 se sembraron dos poblaciones en Villavicencio y se evaluaron por resistencia a enfermedades. 5.4. Selección Recurrente en Costa Rica En 1996 se envió a Costa Rica el acervo genético GPCT-9 y la población PCT-7. Ese mismo año, el Dr. Randolph C. Morera, del Programa del Arroz Nacional, asistió al Curso Internacional de Selección Recurrente en Arroz, realizado en CIA T. Se caracterizó el germoplasma, bajo las condiciones de Costa Rica, seleccionando plantas androesteriles y fértiles separadamente. En 1997 se utilizó el material SO para desarrollo de líneas. Para 1998, los resultados no están todavía disponibles 5.5. Selección Recurrente en El Salvador En 1995 se envió tres poblaciones (PCT-6, PCT-7, y PCT-8) y el acervo GPCT-9 al Centro Nacional de Tecnología Agropecuaria y Forestal (CENTA), donde se llevaron a cabo las actividades siguientes: · Desarrollo de líneas: En 1996 141 y 97 plantas SO se seleccionaron de las poblaciones PCT-7, respectivamente. · Mejoramiento de la Población: basado en la progenie de líneas S2 evaluación y recombinación con el remanente de semillas SO. · Nueva población desarrollada: la población CNA IRAT ES 1/0/2 fue desarrollado por introducción cuatro líneas (X-10, CENTA A-1, CENTA A-2, y CENTA A-5) en la población brasileña CNA IRA T 4/0/6. Para 1998 los resultados no están todavía disponibles 5.6. Selección Recurrente en Panamá En 1996 se envió a Panamá el acervo indica GPCT-9 y población PCT-7. Ese mismo año, el Dr. Ariel E. Jaén Sánchez de la Facultad de Ciencias Agrícolas, Universidad de Panamá, asistió al Curso Internacional de Selección Recurrente en arroz, realizado en CIAT. El introdujo germoplasma sembró y caracterizó. Cosechó plantas fértiles precoces SO para desarrollo de líneas. Para 1998 los resultados no están todavía disponibles 5. 7. Selección Recurrente en Venezuela Después de que asistió al Curso Internacional de Selección Recurrente en arroz, realizado en CIAT, tres poblaciones (PCT-6, PCT-7, y PCT-8) y tres acervos(IRAT 1/420P, IRAT MANA, y GPCT-9) se le envió al Dr. E. Graterol para caracterización bajo las condiciones locales de Calabozo, y estado de Guárico. El· objetivo era seleccionar los mejÓres germoplasmas adaptados e iniciar con el programa de selección recurrente. Dos poblaciones, la PCT-6 y PCT-7, se seleccionaron como fuente de androesterilidad para desarrollar dos poblaciones nuevas locales, identificadas como PFD-1 y PFD-2. Durante 1998 en DANAC-Venezuela, y en CIAT Palmira, se sintetizaron las poblaciones. 5.8. Selección Recurrente en Cuba En 1996 se envió el acervo genético GPIRAT-10 y la población PCT-7 a Cuba, y se caracterizó. La semilla se multiplicó para recombinación. Se seleccionaron plantas fértiles SO para desarrollo de líneas. 5.9. Selección Recurrente en Argentina En el diciembre de 1996 suministramos a la Universidad de Corrientes, las poblaciones PCT- 6\0\0\0, PCT-7\0\0\0, y PCT-8\0\0\0. Se evaluó el germoplasma y se caracterizó. Un total de 171 líneas se seleccionó de las tres poblaciones. Las poblaciones fueron recombinadas y la semilla se multiplicó. La población chilena PQUI-1, se remitió al Universidad del la Plata, para evaluación en el Pampas, región de la provincia de Buenos Aires. Desarrollo de nuevas poblaciones locales: PARG-1, es una población con base genética estrecha para mejoramiento a corto plazo. PARG-2, es una población con variabilidad orientada de PARG-1 introducida en PCT-8 (el mejor germoplasma introducido) PARG-3, proviene de la población PCT8 por introducción de 6 líneas Argentinas. Este se realizará en CIAT Palmira. 5.10. Selección Recurrente en Chile En 1996 se envió a Chile el acervo Japónica GPIRAT-10, desarrollado por GIRAD específicamente para climas templados. Ese mismo año, Dr. Santiago Hernaiz de INIA- Quilamapú asistió al Curso Internacional de Selección Recurrente en Arroz, realizado en CIA T. En 1997 el acervo se caracterizó y se seleccionó plantas SO fértiles para el desarrollo de líneas. Se utilizó también como una fuente de esterilidad masculina, para generar otra población introduciendo cinco líneas chilenas cruzando (Qui. 67108, Diamante, Buli, CINIA 609, y CINIA 606) con plantas androesteriles del acervo. Se envió a CIAT-Palmira parte de la semilla híbrida, obteniendo la generación F1 . Se envió la semilla F2 de regreso a Chile. Se identificó la población básica chilena como PQUI-1 \0\0\0. En 1998 se dividió la población Chilena en dos parte. Una parte se sembró en la localidad de Chillan, y la otra en la localidad de Colchagua. Estos dos sitios corresponden a dos situaciones agroclimáticas diferentes. Después de la recombinación se enviaron a CIAT Palmira las poblaciones para el segundo ciclo de recombinación. 5.11. Selección Recurrente en Uruguay En 1996 se envió a Uruguay el acervo GPIRAT-10. Ese mismo año, el Dr. Fernando Pérez de Vida, asistió al Curso Internacional de Selección Recurrente en Arroz, realizado en CIAT. En 1997 el acervo se sembró, se caracterizó y se seleccionó plantas fértiles SO para el desarrollo de líneas. También se utilizó GPIRAT-10 como una fuente de esteril idad masculina, para desarrollar una población local, cruzando líneas uruguayas con plantas androesteriles de GPIRAT-10. En 1998 en CIAT, se recibió las F1, obtenida de los diferentes cruces para multiplicación de la semilla (F1 generación y cosecha del F2). Tres poblaciones serán obtenidas: PURG-1 de la introducción de 171íneas con grano corto en GPIRAT-10, PURG-2 de la introducción de 60 líneas con calidad del grano largo en GPIRAT-10, y PURG-3 con base genética amplia resultado de la mezcla de las dos poblaciones previas, con las mejores descendencias seleccionadas en GPIRAT-10. 5.12. Desarrollo de la Línea obtenida por cultivo de anteras En 1994 se introdujo de la Guyana francesa, la población IRAT-CT. Esta población viene del mejoramiento del acervo indica GPCNA-18 por cultivo de anteras. En 1995 el laboratorio de cultivo de anteras del CIAT procesó la población IRAT-CT, y desarrolló líneas R2. las líneas fueron evaluadas por FLAR en la Estación Experimental santa Rosa y se seleccionaron cinco líneas. Durante 1998 se evaluaron las cinco líneas y la semilla se multiplicó para evaluaciones futuras. 5.13. Mejoramiento Convencional y Cultivo de anteras para Rumania En el ámbito de la colaboración entre el proyecto de arroz del CIRAD en Montpellier y la institución Rumana FUNDUlEA, dos cruces de Rumania son procesados en el laboratorio de cultivo de anteras en CIAT. las líneas dobles haploides se remitirán a FUNDUlEA, y se guardará unas semillas para enviar a Chile, Argentina y Uruguay. Estas líneas pueden también ser útiles como donadores por tolerancia a frío en las laderas de Colombia. CHAPTERI PRESENTATION l. HIGHLIGHTS • THE CIRAD/CIATIFLAR COLLABORATIVE PROJECT • THE CIAT RICE PROJECT • UPLAND SA V ANNA CONVENTIONAL RICE BREEDING • UPLAND RICE FOR THE HIGHLANDS OF COLOMBIA • RECURRENT SELECTION BREEDING • FONDO LATINOAMERICANO Y DEL CARIBE PARA ARROZ DE RIEGO (FLAR) 2. ACKNOWLEDGMENTS 3. BACKGROUND INFORMATION CHAPTERI PRESENTATION l. IDGHLIGHTS THE CIRAD/CIAT/FLAR COLLABORATIVE PROJECT The third Col/aborative Meeting between CIAT, CIRAD, INRA, and ORSTOM was held at CIAT headquarters in May 1997. At this meeting, the ongoing activities of the CIRADICIAT Rice Col/aborative Project were confirmed, and the project was reinforced by: • The appointment of a new CIRAD-CA scientist at CIAT headquarters. • Starting the adaptation of the AD VENTROP software to Latín America in March. The 4th Collaborative Meeting will be held in 1999 at Montpellier, France. We are working at the implementation of a new collaborative initiative between the CIRAD/CIAT/FLAR rice project and EMBRAPA Arroz e Feijao, Brazil; in rice economics, with the appointment of a CIRAD-CA scientist to Latin America. THE CIAT RICE PROJECT CIAT is developing its research activities according to a project management system. The rice project code is IP-4, and its breakdown structure is presented in Appendix l . The individual work plan of the collaborative project for 1998 is presented in Appendix 2. UPLAND SA V ANNA CONVENTIONAL RICE BREEDING During 1996, the activities developed by the conventional breeding project for upland savanna rice were at first reduced, but then reactivated to a certain extent during 1997. In 1998 we sent upland lines to the new partners we identified during 1997: • Colombia-Ministry of Agriculture for the small farmers of the Atlantic Coast, • Argentina-Universidad Nacional de Tucumán, • Paraguay- Associacion de productores de Arroz de ltapua, • Pero-Programa Nacional de Maíz y Arroz, Pucallpa, and • Venezuela- FONAIAP; and the Universidad Nacional Experimental de los Llanos Orientales "EZEQUIEL ZAMORA". We continue maintaining strong relationships with: • Brazil (EMBRAPA Arroz e Feijao, Goiania), and • Bolivia (CIAT Santa Cruz Bolivia). In the Caribbean, new partners were identified (through CRIDNet), during 1998: Guyana and Belize and Cuba. UPLAND RICE FOR THE IDGHLANDS OF COLOMBIA From 1993, we started, as an informal collaborative effort with the Centro Nacional de Investigaciones de Cafe (CENICAFE) and the CIAT hillsides project, to adapt rice as a new crop for the Colombian highland ecosystem. Results so far are very promising (see "Upland Rice Improvement for the Highlands of Colombia, 1996 Report"). Two upland rice fines were proposed for release. One is a CIAT savanna upland rice for the mid-altitudes (about 1300 mas!, Colombian Coffee Region), and the other is an introduction from Madagascar (CIRADIFOF/FA Highlands Breeding Project) for the higher altitudes (1 600 mas!, Cauca Region). In 1998, we wrote a project that was presented in June to the Colombian institute COLCIENCIAS, by the Centro Internacional de Agricultura Organica (CIAO). The hillsides breeding activities developed during 1998 are presented in the annual report by Michel Vales, now in charge ofthis part ofthe Collaborative Project. RECURRENT SELECTION BREEDING CIAT and CIRAD 's breeding strategies focus on developing and improving populations, and phasing out the development of finished /ines. Such population development and enhancement aim to provide national programs (NARS) with sources ofpotential parents having specific traits. The expertise of the collaborative project on recurrent selection is shared with the NARS through activity reports, didactic documents, field visits, and training courses. The first International Course on Rice Recurrent Selectíon Breeding was held at CIAT in 1996. Fifteen scientists from 13 countries attended the course. Back in their home countries, many began using recurrent selection in their breeding programs. In 1998, two populations developed by the CIRAD/G4I project in Rio Grande do Sul-Brazil, were registered in the germplasm catalog for recurrent selection as PCIRAD-23 and PCIRAD-24. We are monitoring with our partners in Latín America, the use of the basic populations developed by the project. We also help them doing special work at CIA T Palmira; development of specific populations for future local use (Argentina, Uruguay and Venezuela) and generation of recombination cycle (Chile). The first Venezuelan National Course on Rice Recurrent Selection was held at San Felipe - Venezuela, in September 21-26, and organized by Fundacion DANAC, CIRAD/CIA T and EMBRAP A. The Second Intemational Workshop on Rice Recurrent Selection , to be held in Goiania -Brazil, in September 1999 is in preparation. Recurrent selection germplasm crosses continents. On request, we have shipped populations to Europe (France) and Asia (China). FONDO LATINOAMERICANO Y DEL CARIBE PARA ARROZ DE RIEGO (FLAR) (FUND FOR LATINAMERICAN AND THE CARIBBEAN IRRIGATED RICE) In September 1996, CIRAD signed an agreement with the Fund and became a member. Beginning in 1997, the CIRADICIAT collaborative project developed research activities with FLAR on: • Recurren! selection breeding. • Adaptation to Latin America of the CIRAD's ADVENTROP software (Thomas Le Bourgeois, Montpellier- France). • Durable resistance to blast. The activities developed during 1998 are presented in the annual report by Michel V al es, now in charge of this part of the Collaborative Project. In 1998, Bolivia, Guatemala and Uruguay became members of FLAR. Conversations are well engaged with Argentina (S tate of Corrientes), Chile, Ecuador, and Peru to be members in 1999. 2. ACKNOWLEDGMENTS This document reports the research activities developed during 1997 B and 1998 A at CIA T headquarters (Palmira, Department of Valle, Colombia), and "La Libertad" Experiment Station (Villavicencio, Department ofMeta, Colombia). In Colombia, we maintain close collaborative ties with CORPOICA and CENICAFE. But it is not so easy because of the changes occurred in the definition of research activities of these institutions. We could not find out if CORPOPICA Regional 8 in Villavicencio is still interested in doing research in Savanna Upland Rice. For CENICAFE we know that the project named "Diversificacion del cultivo del café" was reduced. That is why we developed the hillsides activities mainly with CIAO who continues to show great interest in the adaptation of Upland Rice in the Colombian hillsides, and with different CIAT's projects (Hillsides and Participatory Research). At the regional level (Latín America), we conduct research activities in close collaboration with scientists of different institutions and universities. We would therefore like to acknowledge the excellent work and collaboration of the following persons: María Antonia Marassi Juan Antonio Marassi Marta Genoveva Nicosia José Alberto Villegas Roger Taboada Paniagua R. Guzmán Orlando Peixoto de Morais Emilio da Maia de Castro Elcio Perpetuo Guimaraes James Taillebois Roberto Alvarado Santiago Ignacio Hemaiz Lagos Remando Delgado Huertas Argentina, Universidad de Corrientes Argentina, Universidad de la Plata Argentina, Universidad de Tucumán Argentina, Universidad de Tucumán Bolivia, CIAT Santa Cruz Bolivia, CIA T Santa Cruz Brazil, EMBRAPA Arroz e Feijao Brazil, EMBRAP A Arroz e F eij a o Brazil, EMBRAPA Arroz e Feijao Brazil, CIRAD/G4I Chile, INIA -Quilamapú Chile, INIA -Quilamapú Colombia, CORPOICA-Regional 8 Randolph Campos Morera Ramón Eduardo Servillón Ariel E. Jaén Sánchez Alberto Herrera G. Eduardo Graterol Fernando Blaz Pérez de Vida Rene Perez Polanco Costa Rica, Ministry of Agriculture El Salvador, CENTA Panamá, Universidad de Panama Venezuela, Universidad (UNILLEZ) Venezuela, DANAC - Fundación Polar Uruguay, INIA-Treinta y Trés Cuba, IIA Sancti-Spiritus 3. BACKGROUND INFORMATION Genetic uniformity, or lack of genetic diversity, is of major concem to breeders, geneticists, and the agricultura! community in general. In many crops, genetic improvement is usually accomplished by reducing genetic diversity in the gene pools used to develop new varieties. But genetic uniformity is now considered as increasing a crop's potential vulnerability to disasters caused by biotic or abiotic constraints. In Latín America, the genetic diversity of rice varieties depends on a small genetic core of landraces (1 in Appendix 6). One way of broadening the genetic base of Latin American rice and assessing the genotype-by-environment interaction is to identify specific potential parents and pool them to develop new, genetically broad- based, breeding material. CIA T and CIRAD' s new breeding strategies focus on developing and improving populations to provide sources of fixed lines or potential parents with specific traits required by national breeding programs. One suitable breeding method to achieve this goal is recurrent selection. Started in 1992, the CIRAD/CIA T rice improvement collaborative project introduced, from Brazil and French Guiana, and developed in Colombia (CIA T Palmira and Villavicencio) gene pools and populations segregating for a male- sterile recessive gene (2 in Appendix 6). At first, he main objectives of the project were: • To understand the performance of the introduced germplasm in the upland acid soils of the Colombian savannas. • To maintain the germplasm by harvesting fecundated male-sterile plants. • To identify adapted fertile genotypes for use in breeding programs for fixed lines. • To start recurrent selection by recombining the best selected genotypes in the introduced germplasm. • To create new populations by incorporating the best locally adapted lines of the CIA T upland-rice breeding program into the best adapted, introduced germplasm. that also provides a good source of male-sterile background. Since 1995, we are mainly focusing with ours regional partners on line development and enhancement of different upland and lowland populations, especially for blast resistance, earliness, tolerance to acid soils and grain yield for upland ecosystem. For lowland conditions (tropical, sub-tropical, and temperate climate) the objectives are resistance to rice blast and Hoja Blaca virus, tolerance to cold, and grain yield and quality. As Intemational Centers, we also develop germplasm with broad genetic base, in order to gather, and maintain variability. To these germplasms we apply a low selection pressure in order to maintain variability and adaptation to broad ecoregional regions. CHAPTERII RECURRENT SELECTION FOR UPLAND SA V ANNA RICE l. INTRODUCTION 2. UPLAND SA V ANNA RICE 2.1. Line Development from Recurrent Populations 2.1.1. Generation S2 2.1.1.1. Populations PCT-5\PHB\1\0,PHB\1 PCT -A \PHB\1 \O,PHB\1 and PCT -4\PHB\1 \1 ,PHB\1 2.1.1.2. Population PCT -4\SA \1\1 2.1.2. Generation S4 2.1.2.1. Populations PCT -5\PHB\1 \0, PCT -A \PHB\1 \0, and PCT -4\PHB\1 \1 2.1.3. Generation S6 2.1.3.1. Populations PCT-5\0\0\0, PCT-A\0\0\0, and PCT -4\0\0\1 2.1.3.2. Population PCT -4\0\0\1>82 2.1.3.3. Populations PCT-5\0\0\0, PCT-A\0\0\0, and PCT- 4\0\0\1. Plant Selection in S3 Lines at PES, 1996 B 2.1.4. Advanced Generations 2.1.4.1. AGs from Populations with a Male-Sterile Gene 2.1.4.2. AGs from Populations without Male-Sterile Gene 3. UPLAND LINE REGISTRATION 4. POPULATION MAINTENANCE THROUGH RECOMBINATION 5. POPULATION ENHANCEMENT BY RECURRENT SELECTION 5.1. Recurrent Selection ,Based on S2 Line Evaluation: Population PCT -4\0\0\1 5.2. Mass Recurrent Selection for Both S ex es for "Hoja Blanca", Blast, and Major Agronomic Traits: Populations PCT-4\0\0\1, PCT-A\0\0\0, and PCT -5\0\0\0 6. DEVELOPMENT OF NEW POPULATIONS 6.1. Upland Savanna Population 6.2. Upland Hillside Population 7. DISTRIBUTING GERMPLASM TO BRAZIL 8. DISTRIBUTING UPLAND-RICE GERMPLASM BRED BY RECURRENT SELECTION 9. USE OF RECURRENT UPLANDGERMPLASM BY LAC P ARTNERS 9.1. Use in Bolivia 9.2. Use in Venezuela 9.3. Use in Cuba 10. TRIALS 10.1 Evaluation of 82 lines from the population PCT -4\SA \1\1 1 0.2. Yield Trial 10.3 INGER-LAC Trial CHAPTERII RECURRENT SELECTION FOR UPLAND SA V ANNA RICE Marc CHÁTEL, Yolima OSPINA, and Jaime BORRERO l. INTRODUCTION The upland rice recurrent selection project aims to adapt, develop, and select upland rice gene pools and populations. The major characteristics that we look for in germplasm for savanna conditions· are: • Tolerance of soil acidity • Resistance to diseases, mainly rice blast (Pyricularia grisea Sacc.) • Resistance to pests, mainly rice plant hopper (Tagosodes orizicolus) • Good grain quality (translucent, long, slender grain) • Early maturity (total cycle about 115 days) 2. UPLAND SA V ANNA RICE The activities we report here were conducted at two experiment stations: • Off season (1997 B): October 1997 to March 1998 at the Palmira Experiment Station (PES) • Cropping season (1998 A): April to September 1998 at "La Libertad" Experiment Station (LES). The soil and climatic characteristics of LES experimental station are presented in tables 1, and 2 2.1. Line Development from Recurrent Populations During the enhancement of gene pools and populations through recurrent selection, we selected fertile plants to develop promising fixed lines or potential parents for regional NARS. 2.1.1. Generation S2 The generation S2 comes from SO fertile plants selected during 1997 A at LES. The generation S 1 was grown during 1997 B, at PES. 2.1.1.1. Populations PCT -5\PHB\1 \O,PHB\1 PCT -A \PHB\1 \O,PHB\1 and PCT -4\PHB\1 \1,PHB\1 Cycles During 1997 A , fertile plants were se/ected for fine development. In each popu/ation PCT-5\PHB\1 \O,PHB\1; PCT-A \PHB\1 \O,PHB\1; and PCT-4\PHB\1\1,PHB\1 , 41, 13~ and 12 SO fertile plants, respective/y, were harvested. During 1997 B, the SI generation was grown at PES. Cropping Season 1998 A The 66 S2lines were evaluated at LES (table 3) and 8 (12%) were selected, (table 4). In each selected line 6 fertile plants were harvested. During 1998 B, the 48 S3 lines (8 families of 6 lines) will be grown at PES. 2.1.1.2. Population PCT -4\SA \1\1 Cycles During 1997 A, I55 fertile plants were selected m the population. The SI generation was grown during 1997 B, at PES. Cropping season 1998 153 S2 lines were eva1uated (table 5) and 65 (42.5%) were se1ected (table 6). 2.1.2. Generation S4 The generation S4 comes from SO fertile plants selected during I996 A at LES, and the selection of S2 lines during I997 A at LES. The generation S3 was grown during I997 B at PES. 2.1.2.1. Populations PCT-5\PHB\1\0, PCT-A\PHB\1\0, and PCT-4\PHB\1\1 Cycles During 1996 A, from the first recurren! selection cycle for leaf blast and "hoja blanca" virus (see I996 report 4-2), we selected 2II SO fertile plants, distributed asfollows: • 49 in PCT-5\PHB\I\0 (II.5% ofthe total number offertile plants) • 48 in PCT-A\PHB\1\0 (I2.4% ofthe total number offertile plants) • II4 in PCT-4\PHB\I\I (I7.3% ofthe total number offertile plants) During 1996 B, the SI generation (2II SI fines) were grown at PES and the S2 seeds sent toLES to grow the S2 generation during I997 A. During 1997 A ,from the 2II S2lines evaluated at LES, 25 were selected. • PCT-5\PHB\I \0 -- I fine selected (2%) • PCT-A\PHB\I\0 -- 2lines selected (4%) • PCT-4\PHB\I \I -- 22 fines selected (19%) In each selected fine, 6 individual plants were selected. During 1997 B, the 150 S31ines (25 families of61ines) were grown at PES. Cropping season 1998 A From the 150 S4 lines evaluated at LES (table 7), 35 (23.3%) were selected (table 8). • PCT -5\PHB\1 \0 no selection • PCT-A\PHB\1\0 no selection • PCT-4\PHB\1\1 35 lines selected (23.3%) In each selected line 6 fertile plants were harvested. During 1998 B, the 210 S51ines (35 families of6lines) will be grown at PES. ¡ t l 2.1.3. Generation S6 The generation S6 comes from fertile SO plants selected during 1995 A at LES. The generations S 1, S3 and S5 were grown during 1995 B, 1996 B and 1997 B, respectively, at PES. The S2 and S4 generations were selected during 1996 A and 1997 A at LES. 2.1.3.1. Populations PCT -5\0\0\0, PCT -A \0\0\0, and PCT -4\0\0\1 Cycles During the 1995 A cropping season at LES, we selected 55, 85, and 18 SO ferti/e p/ants in PCT-5\0\0\0, PCT-A \0\0\0, and PCT-4\0\0\1, respective/y, and during the off-season (1995 B), we grew the Si generation at PES. During the 1996 A cropping season, we observed 158 S2 and 3 checks (Oryzica Sabana 6, IAC 165, and CIRAD 409) at LES, and selected mainly for p/ant type and yie/d potential, discarding 102 S2 lines (64.5%). A total of 56 S2 lines (35.4%) were se/ected: • PCT-5\0\0\0 --21 lines (38.1 %) • PCT-A\0\0\0 --26lines (30.6%) • PCT-4\0\0\1 -- 9 lines (50. 0%) From the 56 selected lines, we harvested 178 fertile plants: 62 from PCT-5\0\0\0, 91 from PCT-A \0\0\0, and 25 from PCT-4\0\0\1. We app/ied different selection intensity to each selected S2 line, according to the phenotypic value of the /ines (grain yield potential, and plant and grain type). For example, the highest average selection intensity in three PCT-5\0\0\0 S2 lines was 14% and the lowest average was l . 6% in 14 S2 lines. The S3 generation was grown during 1996 B at PES and the S4 seeds sent toLES, to grow the S4 generation during 1997 A. During 1997 A,from the 178 S4 lines evaluated, 47 were selected • PCT-5\0\0\0 -- 3 lines se/ected (5%) • PCT-A \0\0\0 -- 35 Unes selected (38%) • PCT-4\0\0\1 -- 9lines se/ected (36%) From each selected line, 6 individual plants were selected. During 1997 B, the 282 S5 lines (47 families of6lines) were grown at PES. Cropping Season 1998 A From the 282 S6lines (table 9), 64 (22.7%) were selected (table 10). • PCT -5\0\0\0 no selection • PCT-A\0\0\0 45 lines selected (16.0%) • PCT-4\0\0\1 19lines selected (6.7%) In each selected line 6 fertile plants were harvested. During 1998 B, the 384 S7lines (64 fami1ies of 6lines) will be grown at PES. 2.1.3.2. Population PCT -4\0\0\1>82 Cycles During 1996 A, we started enhancing this population by first evaluating the S2line. We took advantage of the 1996 S2 fine tria/ to select S2 fines and individual fertile plants for fine development. From 152 S2 fines evaluated, we selected 19 (1 2.5%) and 7 4 individual plants, based on plant and grain type, and grain yield potential. During 1996 B, the S3 generation was grown at PES and the S4 seeds were sent to LES to grow the S4 generation during 1997 A. During 1997 A,from the 74 S4fines evaluated, 16 were selected (22%). In each selected fine, we harvested 6 individual plants. During 1997 B, the 96 S5 fines (1 6 famifies of 6 fines) were grown at PES. Cropping Season 1998 A From the 96 S6lines evaluated (table 11), 12 (12.5%) were selected (table 12). In each se1ected line 6 fertile plants were harvested. During 1998 B, the 72 S7lines (12 families of 6 lines) will be grown at PES. ! t l 1 1 f l 1 2.1.3.3. Populations PCT-5\0\0\0, PCT-A\0\0\0, and PCT-4\0\0\1. Plant Selection in S3 Lines at PES, 1996 B Cycles During 1996 B, at PES, we selected 12 individual fertile plants, with suitable characteristics, from S3 lines. The S4 seed was sown during 1997 A at LES. During 1997 A, from the 12 S4 fines evaluated, only 3 were selected in one population: • PCT-5\0\0\0 -- no seleciion • PCT-A \0\0\0 -- no selection • PCT-4\0\0\1 -- 3 lines selected (75%) In each selected line, 6 individual plants were selected. During 1997 B, the 18 S5lines (3 families of6lines) were grown at PES. Cropping season 1998A. The 18 lines were evaluated at LES (table 13), and 2 (11.1 %) were selected (table 14). 2.1.4. Advanced Generations The advanced generations (AGs) carne from the SO fertile plants selected from the germplasm we introduced in 1992 from Brazil (with male-sterile gene) and from the gene pool and populations previously developed at CIA T (no male-sterile gene). 2.1.4.1. AGs from Populations with a Male-Sterile Gene Cycles During 1995 B, at PES, we increased seed of2 and 4 advanced lines selectedfrom CNA-IRAT 5 and CNA-IRAT A, respective/y. During 1996 A, we observed these 6 fines at LES. From each of the 6 lines, we selected 5 individual p/ants. During 1996 B, we increased seed of the 30 plants at PESto set up a yield tria! during 1997 A. During 1997 A, a yield tria/ was conducted and analyzed.8 lines presented a high yield potential and good milling characteristics. Cropping Season 1998 A, the best lines , (tables 15 and 16) were used to set-up the VIO AL acid soil nursery of INGER-LAC, to be dispatched to ours partners and for registration appliance in the Rice catalogue of CIRAD. 2.1.4.2. AGs from Populations without Male-Sterile Gene Cycles The first lowland populations used in recurren! selection breeding had been developed by manual crossing by the CIAT Rice Program in the early 1990s (Drs. E. P. Guimariies and F. Correa). The populations were developed from Indica and Japonica parents and used to target blast resistance. One gene pool and three populations were registered in the recurren! selection catalogas GPCT-1, PCT-2, and PCT-3 (Appendix 7). Fixed lines were selected from GPCT-1 and PCT-3 at the Santa Rosa Experiment Station (a "hot spot"for blast evaluation). In 1996 A, we selected 89 individual plants showing good characteristics for savanna conditions. During 1997 A, the 89 progenies were evaluated under savanna, acid-soil conditions at LES. A total of 36 lines were selected. Because these lines come from an Indica-Japonica recombination, Dr. J. Gibbons from FLAR shows interest in this material as having poten tia/ for lowland conditions. Cropping Season 1998 A Four (4) 1ines from PCT-3, showing very good adaptation to acid soil condition were eva1uated and seed increased at LES, (table 17). 3. UPLAND LINE REGISTRATION CIAT does not register lines: when a specific line does well in a given country, the national institution of that country may decide to name and release it for commercial cultivation. CIRAD has a mechanism by which breeders may register a specific material in a catalog. The line is named CIRAD ( and is also given its local synonym, if it is the result of collaborative work), and is registered as "working material". Cycles During 1996, two advanced lines--CNA-IRAT 5 \SAI0\3>127-2-M-2-M and CNA- IRAT A\SAI0\3>1-M-2-M-4-M, selected from the populations CNA-IRAT 5 and CNA-IRAT A--were proposedfor registration in the CIRAD rice catalog. They are registered as CIRAD 410 and CIARD 411, respective/y. During 1997, the results of a yield tria/ showed that 3 fines were very promising. They were selected from two recurren! populations. They were remitted to INGER LA C to be part of the VIO AL tria/ for acid soil condition. During 1998, we apply for their .registration in the CIRAD rice catalog. 4. POPULATION MAINTENANCE THROUGH RECOMBINATION Cycles Until now, the upland populations were maintained under irrigated conditions at Palmira. But, results obtained in Madagascar under similar conditions show that a possible genetic drift toward an increased frequency of the Indica plan! type may occur in the population. Such a drift can be explained by a more effective cross- pollination among genotypes with an Indica background. We must remember that the male-sterile line used to build up populations is an irrigated Indica line (IR 36 male-sterile mutan!). During the 1996 A cropping season, we decided to maintain and increase seed of upland populations under savanna conditions. We maintained the following 6 populations: CNA-IRAT 5/0/4, CNA-IRAT A/012, CNA-IRAT P/1/1, PCT-AI0\0\0, PCT-5\0\010, and PCT-410\0\1. Al/ male-sterile plants were identified, harvested individual/y, and their seeds mixed in equal proportions. Fertile plants were also harvested individual/y and their seeds mixed in equal proportions. The populations were sent to CIAT Palmira, and stored in a cold chamber until further use by the project or requested by regional NARS breeding programs. During 1997 and 1998, no new maintenance ofrecurrent germplasm was made. 5. POPULATION ENHANCEMENT BY RECURRENT SELECTION The CIA T rice project emphasizes the enhancement of populations and is phasing out the production of fixed lines for direct release by the NARS of the region. The strategy is to develop and enhanc~ gene pools and populations for well-targeted traits for use as sources of potential parents by national breeding programs. In the first 2 years ofthe recurrent selection project, we concentrated on introducing germplasm from Brazil (EMBRAP A Arroz e Feijao and CIRAD) and French Guiana, and characterizing and mass selecting it. From 1995 onward, we concentrated our activities on enhancing and developing new populations. 5.1. Recurrent Selection Based on 82 Line Evaluation: Population PCT -4\0\0\1 Cycles During 1995 A, at LES, 159 SO fertile plants were selected. During 1995 B, the SI generation was grown at PES. During 1996 A, we started the first recurrent selection cycle: • Evaluation: 152 fines of S2 and 2 checks (Oryzica Sabana 6 and CIRAD 409) were evaluated and selected at LES under the "Augmented Blocks" statistical design (7, Appendix 6). • Selection: Results of the S2 tria! were analyzed and 53 S2 fines were selected. • Recombination: In 1996 B, at PES, remaining seeds from the SO plants from which originated the selected S2 lines were mixed and grown to develop the recombined enhanced population. • ldentification: The enhanced recombined population was identified as PCT-4\SA \1\1. During 1997 A, the population PCT-4\SA\1\1 was grown at LES to go through a second selection cycle. Harvest of Male-Sterile Plants. Male-sterile plants were harvested individual/y and their seeds mixed in equal proportions to complete the second cycle of recombination of the population selected one time. The second cycle of recombination is identified as PCT-4\SA \2\1. Seed will be stored in the cold chamber for future use. Selection of Fertile Plants. A total of 155 SO plants were selected, anda sample of each SO seed was stored in the cold chamber. During 1997 B, the S 1 generation was grown at PES, and S2 seeds harvested. Cropping Season 1998 A From the 155 S2 lines, 152 were evaluated during 1998 A at LES in a trial named "Augmented B1ocs of Federrer" (BAF), table 5. With a selection index of 39.5o/o we selected the 60 best lines (table 18) for recombination from the original SO selected plants. The recombination will be made at PES during 1998 B by the sowing of the balanced mixture of SO seed (equal proportion of seed of each SO plant) and harvest of the seeds produced by the mal e sterile plants. Multilocal evaluation of S2 's lines The S2 set of lines was remitted to Brazil (EMBRAPA Arroz e Feijao), Bolivia (CIAT Santa Cruz), and Venezuela (UNELLEZ) for evaluation and selection for line development. Selection of SO plants in the second cycle of recombination, after one selection for acid soil. Seventy five (75) SO fertile plants from the population PCT-4\SA\2\1 were selected at LES, (table 19). The generation S 1 will be grown during 1998 B, at PES. 5.2. Mass Recurrent Selection for Both Sexes for "Hoja Blanca", Blast, and Major Agronomic Traits: Populations PCT-4\0\0\1, PCT-A\0\0\0, and PCT -5\0\0\0 Cycles During 1995 A, at LES, we eliminated at the vegetative stage al/ plants showing symptoms of leaf blast and HBV. At harvest, we selected male-fertile plants. Seeds produced by these plants were the result of fertilization with po/len produced by healthy fertile plants. We selected 102, 99, and 96 male-sterile plantsfrom PCT- 5\0\0\0, PCT-A\0\0\0, and PCT-4\0\0\1, respective/y, and their seeds were mixed in equal proportions. The first mass recurren! selection cycles (selection and recombination) were identified as PCT-5\PHB\1\0, PCT-A\PHB\1\0 and PCT-4\PHB\1\1, respective/y. During 1996 A, the seed mixture of each population with one mass recurren! selection cycle was grown at LES. To develop the second recurren! selection cycle, the same selection method as that used during 1995 A was applied. We selected 304, 341, and 442 healthy male- sterile plants, fertilized with pollen of fertile healthy plants, from PCT-5\PHB\1 \0, PCT-A\PHB\1\0, and PCT-4\PHB\1\1, respective/y, and mixed their seeds in equal proportions. The second mass recurren! selection cycles (selection and recombination) were identified as PCT-5\PHB\1 \O,PHB\1, PCT-A \PHB\1 \O,PHB\1, and PCT- 4\PHB\1\1,PHB\1, respective/y. · During 1997 A, the seed mixture of each population with two mass recurren! selection cycles was grown at LES. To make the third recurren! selection cycle, the same selection method as that used during 1995 A and 1996 A was applied (al! plants with symptoms of leaf blast and "hoja blanca" were eliminated during their vegetative stage) . We selected 218, 253, and 165 healthy male-sterile plants, fertilized with po/len from fertile healthy plants, from PCT-5\PHB\1\0,PHB\1; PCT-A\PHB\1\0,PHB\1; and PCT- 4\PHB\1\1,PHB\1, respective/y, and their seeds mixed in equal proportions. The third mass recurren! selection cycles (selection and recombination) are identified as PCT-5\PHB\1\0,PHB\1,PHB\1; PCT-A\PHB\1\0,PHB\1,PHB\1; and PCT-4\PHB\1 \1,PHB\1,PHB\1. Cropping Season 1998 A Fourth Cycle of Recurrent Selection The seed mixture of each population with 3 mass recurrent selection cycles was grown at LES. To make the fourth recurrent selection cycle, the same selection method as that used during 1995 A, 1996 A and 1997 A was applied (all plants with symptoms ofleafblast and "hoja blanca" were eliminated during their vegetative stage). We selected 180, 200, and 240 healthy male-sterile p1ants, fertilized with pollen from ferti1e healthy plants, from PCT-5\PHB\1\0,PHB\ 1,PHB\1; PCT-A\PHB\1\0,PHB\1, PHB\1; and PCT-4\PHB\1\ l,PHB\ 1, PHB\1 respectively, and their seeds mixed in equal proportions. The fourth mass recurrent selection cycles (selection and recombination) are identified as PCT-5\PHB\ 1\0,PHB\1,PHB\1, PHB\ 1; PCTA\PHB\1\0,PHB\1,PHB\, PHB\1; and PCT-4\PHB\1\1,PHB\1,PHB\1, PHB\1. Selection of SO fertile plants 30, 24, and 55 fertile plants were selected in the respective populations PCT -5\PHB\1 \O,PHB\ 1 ,PHB\1; PCT -A \PHB\1 \O,PHB\ 1 ,PHB\; and PCT-4\PHB\1\ l,PHB\l,PHB\ 1 for the future development oflines (tables 20, 21 and 22) The generation S 1 will be grown during 1998 B at PES. 6. DEVELOPMENT OF NEW POPULATIONS The development of new populations is a major activity of the project, and provides the main source of new recombined variability for population enhancement and line development. We need to be well focused in our choice of variability and recombine in new germplasm, as well as in the source of male sterility available 1 (usually a well-adapted existing population or gene pool). l In 1996 B, we decided to build up at PES two new Japonica populations, targeting f upland savannas and hillsides. The source of male-sterility background is the best ! Japonica population previously developed by the project. 6.1. Upland Savanna Population The idea behind developing that population is to pool the best lines from the CIA T conventional rice breeding project and the commercial varieties released in Brazil, Colombia, and Bolivia. Cycles In 1996 B, 18 lines were selected according to their performance for early maturity, blast and acid-soil tolerance, and grain quality. Male-sterile plants from the best- adapted upland Japonica population (PCT-4) were used as female parents. Each line was eros sed with at least four mal e sterile-plants of the population PCT-4. During 1997 A, at PES, each resulting F1 was grown individual/y, evaluated, and individual plants selected. The F2 seed of the selected F 1 plants were bulked in equal proportions. Each F2 bulk was mixed in balanced proportions to build up a new basic population, identified as PCT-11 \0\0\0. During 1997 B, at PES, the basic population will be recombined once. The first cycle of recombination of the basic population will be identified as PCT -11\0\0\ l. Cropping Season 1998 A The PCT -11 Popu1ation was grown at LES starting its evaluation and selection. 95 So fertile plants were selected for future line development (table 23). During 1998 B, the 95 S 1 's were planted at PES, and the S2 seed harvested. 6.2. Upland Hillside Population The idea is to develop a population for the Andean highlands of Colombia, with early maturity, cold tolerance, and adaptability to high altitudes (1300-1600 masl). Cycles In 1996 B, 11 lines--6 from the CIRADIFOFIF A hillsides program of Madagascar, 4 from the CIAT upland savannas program, and 1 IRAT line--were selected according to their previous evaluations at high altitudes for early maturity and spikelet fertility. We used the best-adapted upland Japonica population (PCT-4) as a source for mal e sterility. Each line was eros sed with at least 4 male-sterile plants of PCT-4. During 1997 A, at PES, each resulting F1 generation was grown individual/y, evaluated, and individual plants selected. The F2 seeds of the selected F 1 plants were bulked in equal proportions. Each F2 bu/k was mixed in balanced proportions to build up the new basic population, identified as PCT-13\0\0\0. During 1997 B, at PES, the basic population will be recombined once. The first cycle of recombination of the basic population will be identified as PCT-1 3\0\0\1. Cropping Season 1998 A The second cycle of recombination of the population was made at PES and the recombined population will be remitted to M. Vales for evaluation at medium to high a1titude in the Colombian Andes. 7. DISTRIBUTING GERMPLASM TO BRAZIL Breeding lines were sent to EMBRAP A Arroz e Feijao for evaluation and selection. Unfortunately for unknown reasons, the lines were not delivered to EMBRAP A Rice and Beans Center by EMBRAP A CENAR GEN and we don 't have enough seed for an other shipment. 8. DISTRIBUTING UPLAND-RICE GERMPLASM BRED BY RECURRENTSELECTION Since 1995 we started to release recurrent populations and gene pools to NARS in Latin America, W est Africa, and Asia. 9. USE OF RECURRENT UPLANDGERMPLASM BY LAC P ARTNERS 9.1. Use in Bolivia R. GUZMAN, R. TABOADA, M. CHATEL, Y. OSPINA and J. BORRERO The populations PCT -4 and PCT-5 from CIA T, were characterized. The population PCT -4 is better adapted to the local conditions. Mass selection of steri1e plants was made in the two populations with a respective intensity of selection of 31 and 33% Population breeding through recurrent selection will be continued with the PCT -4 population. Fertile plants, to develop fixed lines were selected into the population PCT-4. The population PCNA-16 (CNA-7) introduced from EMBRAPA, Arroz e Feijao, Brazil, was characterized. Mass selection was made at vegetative stage for leaf blast and after flowering for neck blast. 13 plants with good agronomic characteristics were selected to develop fixed 1ines 9.2 Use in Venezuela Alberto HERRERA, Marc CHÁTEL, Yolima OSPINA, and Jaime BORRERO We sent to UNELLEZ, Venezuela the population PCT -11 in order to initiate a project on recurrent selection in upland rice. The population was observed and characterized. lt is a very promising germplasm. 9.3 Use in Cuba Rene PEREZ POLANCO, Marc CHATEL, Yolima OSPINA, and Jaime BORRERO (Rene Perez Polanco attended the first International course on recurrent selection held at CIA T in 1996). The results reported here are from the 1996 and 1997 cropping seasons. In 1996, the population PCT -4 was introduced to Cuba and characterized for plant type, tillering, plant heigh, heading time, grain type and resistance to rice blast. By harvesting the male-sterile plants the germplasm was recombined and seed increased. In 1997, the germplasm was recombined a second time, and 19 SO fertile plants were selected for line development. For developing a local population for upland condition for tillering hability, grain yield and later heading time, the population PCT -4 was introgresed by 4 Cuban lines (IACuba-14, IACuba-19, IACuba-20, and J-104). Crosses were made between the different lines and male-sterile plants of PCT -4 using the methodology, leamed at CIA T, during the recurrent selection course. 10. TRIALS 10.1. Evaluation of S2 Lines from PCT-4\SA\1\1 155 S2 lines selected in the population PCT-1\SA\1\1 were evaluated using a statistical trial named "Augmented ( or hoonuiahu) designs. The trial is made of 8 blocks in witch are randomly distributed 19 S2 lines and 3 checks (0. Sabana 6, O. Sabana 10 and CIRAD 409). Each block is fenced by rows ofblast spreaders. The same trial was sent to Brazil, Bolivia and Venezuela. The results of the trial conducted in Colombia are presented in the table 18. 10.2. Yield Trial · 8 advanced lines developed by the project were evaluated for different agronomic traits and yield potential. The results are presented in the table 24. 10.3. INGER-LAC Trial The 1998 VIOAL "Suelos Acidos" is made of 31 lines coming from our project, (table 15), and was dispatched to different countries in Latin America. The results in the different countries will be reported by INGER- LAC. CHAPTERlll CONVENTIONAL BREEDING FOR UPLAND SA V ANNA RICE l. SAV ANNA UPLAND RICE 1.1. Use of CIA T/CIRAD Savanna Lines in Brazil 1.2. Line release in Brazil 1.3. Line release in Bolivia 1.4. Use of CT and IRAT Lines in China 1.5. Evaluation of W ARDA's lines 2. IDGHLAND UPLAND RICE 2.1. History 2.2. Coffee Region - CENICAFE and CIAO 2.3. Department of Cauca and Central America CHAPTERIII CONVENTIONAL BREEDING FOR UPLAND SA V ANNA RICE l. SA V ANNA UPLAND RICE Marc CHATEL, Yolima OSPINA, and Jaime BORRERO As was stated earlier, we are gradually phasing out most of the activities involved in the development offixed lines for direct release by NARS. In 1996 B, we sent savanna upland lines (F4 and F5 generation) to EMBRAPA Arroz e Feijao for observation and seed increase. These lines were sent back to CIAT, Palmira, in 1997. In 1997 B, the lines were seed increased and dispatched to different countries. In Colombia, we continue the eva1uation and selection of lines for the development of fixed material to be tested by CORPOICA in the "Altillanura" condition. During 1998 A, we selected 24 lines that presented the best characteristics of adaptation to acid soil condition (table 25). 1.1. Use of CIAT/CIRAD Savanna Lines in Brazil During our visit to EMBRAPA Arroz e Feijao, we had the opportunity to track back the use of CIAT lines in the breeding program of this Center. The results of the survey, for the 1997/1998 cropping season, showed that the CIAT/CIRAD savanna materials continue to be very useful for the Brazilians at each step of their breeding program. The participation of CIA T/CIRAD material in the different trials is expressive: 89% in the advanced trials, 28 % in the preliminary trials, and 19% in the observation trials. The main characteristic the Brazilian praised from CIRAD/CIA T material is earlyness, plant and grain type. Trial type Nbr. of accessions CIAT/CIRAD Participation Observa~aoEO-S 168 26 15% Observa~aoEO-SF 176 39 22% Preliminar 11 22 5 23% Preliminar 111 24 8 33% Avan~ado 11 15 13 87% A van~ado M. T 2 2 100% A van~ado 111 12 11 92% 1.2. Line release in Brazil During the period 1994-1997, 4 lines were released in different States of Brazil. They are: CONFIAN<;A (S tates of Roraima and Minas Gerais ), CANASTRA (States of Minas Gerais, Goias, Tocantins, Piaui and Maranhao), MARA VILHA (Goias, Mato Grosso, Tocantins,Para, Roraima and Rondonia), and PRIMA VERA (S tates of Goias, Tocantins, Maranhao, Piaui, Mato Grosso and Mato Grosso do Sul). From these 4 released lines, 2 comes from the CIA T breeding program: CANASTRA CT 7415-6-5-1-2-B MARA VILHA CT 6516-23-10-1-2-2-B Three new CIAT lines are very promising candidates to be released in 1999: CNA8172 CT 11614-1-4-1-M CNA8305 CT 11251-7-2-M-M CNA8436 CT 11251-7-2-M-1-M-M 1.3. Line release in Bolivia A CIRAD line (IRA T 170) is to be released in Bolivia. 1.4. Use ofCT and IRAT Lines in China In 1995, we sent China the first set of savanna lines from Brazil and CIAT/CIRAD as part of a collaboration between the Foods Crops Research Institute of the Yunnan Academy of Agricultura! Science, Kunming (Y AAS) and CIRAD. Preliminary results are highly promising, showing good immediate adaptation and acceptability. In 1997, Dr. Tao Dayun told us that the savanna upland line CT 9278-11-14-2-1-M was very promising as parent for the Chinese upland breeding program. In 1998, we were invited to China to a monitoring tour. New CT lines are under evaluation in different trials and sites. The rice project of CIRAD Montpellier has el ose links with Y AAS and have shipped many upland lines in the recent years. After screening, they are used as source of progenitors or for direct release. The line IRAT 104 was released in 1996. In 1998, the line IRA T 359 is conducted in demonstration fields, and will be released in 1999. 1.5. Evaluation of W ARDA's Unes From the 100 lines we received and planted at LES, 8 were selected. They present good vigor, acid soil tolerance and acceptable grain type (table 27). 2. IDG~AND UPLAND RICE Marc CHATEL, Michel VALES and Jaime BORRERO The Andean Mountains range across Colombia from south to north, rising to almost as high as 6000 masl. The most important agricultura! activity in the mid-altitudes (1000-2000 masl) ofthis area is coffee, planted by small farmers. This crop takes at least 3 years to reach commercial. productivity, but, in the meantime, farmers must use considerable resources to control weeds and prevent erosion. With this cropping system in mind, CENICAFE has been working on different altematives for crop diversification to help farmers earn income while waiting for the coffee to reach commercial productivity. Another area of significant agricultura! achVIty by small farmers is in the Department of Cauca, southwestem Colombia, where new crops are being incorporated into existing cropping systems or new ones developed by CIA T to ensure local food security. 2.1. History To identify upland germplasm adapted to the hillside areas of Colombia, the CIAT/CIRAD rice project, together with CENICAFE, started, in 1993, to evaluate 31 selected savanna lines in the heart of the coffee-growing area, at 1300 mas/. Climatic data collected at the main site show annual average temperatures ranging from 23.1 to 20.6 °C. The monthly average maximum (28.5 °C) occurs in February and the mínimum (16. 9 °C) in September. The germplasm for this region must therefore tolerate cold (i.e., have high spikeletfertility). The lines used for the first tria/ were selected from the savanna upland germplasm collection at CIAT. Selection was based on knowledge previously gained from the CIRAD/FOFIF A Highland Rice Project in Madagascar. Upland lines must be early maturing and tolerate cold (as measured by paniclefertility). Results obtained in La Catalina, Department of Risaralda, showed that the percentages for empty grains ranged from almost 100% to 12%, indicating that the germplasm presented variability for cold tolerance. Growing period extended to about 150 days after sowing (DAS), compared with 120 DAS under savanna conditions. Selection concentrated on lines with at least 60% fertility. Th e average grain yield of the six best-adapted lines was higher than e:x:pected, ranging from 3775 to 5592 kg/ha. 2.2. Coffee Region - CENICAFE and CIAO In 1993, upland lines developed by CIRADIFOFIFA for the highlands of Madagascar were introduced to Colombia and seed increased. The new germplasm was distributed to CENICAFE and the hillside project at CIAT. In 1994, line evaluation started in the Department ofCauca. In 1995, the Centro Internacional de Agricultura Orgánica (CIAO) began evaluations at 1600 mas/. The first results were presented at the Conference on Rice for the Highlands in Madagascar in April 1996. In 1997, in the Coffee Region, we identified with CENICAFE and CIAO, the line CT 10069-27-3-1-4, with e:x:cellent adaptation to the mid-altitudes. Considering the potential of this line over time (average yield grain production of 4 t/ha), we decided to register it in the CIRAD Rice Catalog. Germplasm Introduction. Forty-one new lines were introduced from Madagascar to Colombia and seed increased at CIAT, Palmira, and then dispatched to CENICAFE, CIAO, and CIAT's hillsides project. Crosses. Eleven single crosses were made at PES between line CT 10069-27-3-1-4 and 1 O lines from Madagascar and CIRAD, previously selected for their good performance under highland conditions. The F1 generation was grown during 1997 A at PES, and the F2 seed sent to our partners. Cropping Season 1998 Dr. Michel Vales from CIRAD, arrives at CIA T in August 1997 and took the responsibility of the breeding activities for the Highlands of Latin America. For more information about the activities developed during 1998, report to his annual report. 2.3. Department of Cauca and Central America Cropping season 1997 In the Department of Cauca, the five best lines were selected last year and, with one savanna upland check (CIRAD 409), tested this year on farm by five smallholders The best line (Latsidahy/FOFIF A 62-3) from last year 's experiment was also the best in this year 's on-farm tria!, with an average grain production of 1400 kg/ha at 1600 mas!. The savanna upland check showed complete sterility at each farm. 1f these results are conjirmed in this semester 's trials, we will regís ter /in e Latsidahy/FOFIF A 62-3 in the CIRAD Rice Catalog. A survey was conducted with the five farmers to know what are the most desirable characteristics of a rice line. Ranking at first and second places, respective/y, are a high number of panicles and a short cycle. At the vegetative stage, the line that scored as having the highest acceptability was also the one that had the highest yield. The same set of 41 lines introducedfrom Madagascar was dispatched to the CIAT hillsides project for testing in Colombia and Central America. Cropping Season 1998 Dr. Michel Vales from CIRAD, arrives at CIAT in August 1997 and took the responsibility of the breeding activities for the Highlands of Latin America. For more information about the research activities developed during 1998, report to his annual report. CHAPTERIV RECURRENT SELECTION FOR LOWLAND RICE l. INTRODUCTION 2. FLAR/CIRAD RECURRENT SELECTION for "Hoja Blanca" 3. RECURRENT SELECTION IN COLOMBIA 4. RECURRENT SELECTION IN COSTA RICA 5. RECURRENT SELECTION IN EL SALVADOR 6. RECURRENT SELECTION IN PANAMA 7. RECURRENT SELECTION IN VENEZUELA 8. RECURRENT SELECTION IN CUBA 9. RECURRENT SELECTION IN THE SOUTHERN CONE 9.1. Recurrent Selection in Argentina 9.2. Recurrent Selection in Chile 9.3. Recurrent Selection in Uruguay 9.4. Recurrent Selection by CIRAD, in Rio Grande do Sul, Brazil 10. MAINTAINING GERMPLASM BRED BY RECURRENT SELECTION 11. DISTRIBUTING LOWLAND-RICE GERMPLASM BRED BY RECURRENTSELECTION 12. REGISTERING NEW POPULATIONS 13. LINE DEVELOPMENT THROUGH ANTHER CULTURE 14. RECURRENT SELECTION AND ANTHER CULTURE FOR CIDLE 15. ANTHER CULTURE FOR ROMANIA CHAPTERIV RECURRENT SELECTION FOR LOWLAND RICE l. INTRODUCTION The recurrent selection breeding project started by introducing different gene pools and populations developed in Brazil to Colombia by EMBRAPA Arroz e Feijao and CIRAD and to French Guiana by CIRAD. The germplasm was characterized at CIA T, Palmira, and the best-adapted populations were used to develop new populations by introducing new variability. This resulted in three populations that were registered in the recurrent selection catalogas PCT-6, PCT-7, and PCT-8. This work was conducted at CIAT in close collaboration with Drs. C. Martínez andE. P. Guimaraes. A gene pool was also built up, using a different source for the gene ofmale sterility. The gene pool was registered as GPCT-9. A second gene pool, developed by CIRAD for temperate climates, was registered as GPIRAT-10. In 1998, two populations developed by CIRAD in collaboration with G4I, in Brazil, were regeistered as PCIRAD-23 and PCIRAD-24. Descriptions of these populations and gene pools are presented in Appendix 3. 2. FLAR/CIRAD RECURRENT SELECTION: Recurrent Selection on both sexes for "Hoja Blanca Virus" Jaime BORRERO, Marc CHATEL, James GIBBONS, and Monica TRIANA lntroduction One of FLAR's objectives is to focus on breeding. Recurrent selection is an alternative method to conventional breeding and can be incorporated into FLAR's breeding activities. W e applied the recurrent selection breeding method to existing germplasm for resistance to the "hoja blanca" virus vector (rice plant hopper, Tagosodes orizicolus ) and the blast fungus, both considered as the most important biotic problems in the tropics. Cropping Season 1997 Three populations, PCT-6\0\0\2, PCT-7\0\0\0, and PCT-8\0\0\0 and the gene pool GPCT-9\0\0\0F were evaluated for resistan ce to the "hoja blanca " virus according to the methodology developed by CIA T. Each germplasm material and check were sown in the "hoja blanca" nursery. At 45 days after sowing, the populations and checks were evaluated and the number of healthy and diseased plants counted. In the nursery, the four original germplasm materials showed intermediate susceptibility to "hoja blanca" (the same leve! as that ofthe check Oryzica 1). After transplanting, PCT-7 and GPCT-9 presented a high number of plants with "hoja blanca" symptoms. The two least susceptible germplasm materials were PCT-7 and PCT-8, with 18% and 19% of immune plants, respective/y. These plants will be recombined to complete the first cycle of recombination. Healthy plants of each germplasm material were transplanted separately for recombination with male-sterile plants. The selected populations were PCT- 6\HB\0\2, PCT-7\HB\0\0, PCT-8\HB\0\0, and GPCT-9\HB\0\0F. The recombined populations, after the first cycle of·selection, were identified as PCT-6\HB\1\2, PCT-7\HB\1 \0, PCT-8\HB\1 \0, and GPCT-9\HB\1 \O F. Cropping Season 1998 Two more recurrent cycles were performed at PES for PCT -7 and PCT -8 . The Population PCT-6 after two cycles ofrecurrence was remitted to Dr. Michel Vales. This enhanced germplasm is the starting point ofhis recurrent selection breeding project for partial Resistance to rice blast. 3. RECURRENT SELECTION IN COLOMBIA Hernando DELGADO, Marc CHATEL, and Yolima OSPINA Last year we sent four germplasm materials (PCT-6\0\0\2, PCT-7\0\0\0, and PCT- 8\0\0\0 populations and the gene pool GPCT-9\0\0\0F) to CORPOICA Regional 8. Each material was grown separately at LES for recombination, characterization, and se/ection of SO fertile plants for line development. The four materials performed well, with the PCT-6 and PCT-7 populations presenting the best poten tia/. Next year, these populations wi/1 be eva/uated for blast resistance at the Santa Rosa Experiment Station. Cropping Season 1998: The populations were sown in Villavicencio, and the results are not yet available. 4. RECURRENT SELECTION IN COSTA RICA Randolph C. MORERA, Marc CHATEL, and Jaime BORRERO In 1996, we sent Costa Rica the Indica gene pool GPCT-9 and the population PCT-7. That same year, Dr. Randolph C. Morera of the National Rice Program attended the International Course on Rice Recurrent Selection Breeding held at CIAT. The germplasm was characterized under Costa Rican conditions and maintained by harvesting male-sterile and fertile plants independently. In 1997, the germplasm was used for fine development by selecting SO fertile plants. Cropping Season 1998: The results are not yet available. 5. RECURRENT SELECTION IN EL SALVADOR Ramón Eduardo SERVILLON, Marc CHATEL, and Jaime BORRERO In 1995, we sent three populations (PCT-6, PCT-7, and PCT-8) and the gene pool GPCT-9 to the Centro Nacional de Tecnología Agropecuaria y Floresta! (CENTA), El Salvador. Line development. In 1996, 141 and 97 SO fertile plants were selected from the PCT-7 and PCT-8 populations, respective/y. Population enhancement for grain yield, plant type, blast resistance, and grain quality. The recurrent selection method used is based on S2 progeny evaluation and recombinationfrom the remaining SO seeds. One hundred S2/inesfrom the PCT-7 population were evaluated at two different sites. New population development. The population CNA /RAT ES 11012 was developed by introducing 4 lines (X-10, CENTA A-1, CENTA A-2, and CENTA A-5) into the Brazilian population CAN IRAT 41016. The new population has already passed through 2 cycles of recombination and SO fertile p/ants were selected during 1997 B. Cropping Season 1998: The results are not yet available. :1 6. RECURRENT SELECTION IN PANAMA Ariel E. JAÉN SANCHEZ, Marc CHATEL, and Jaime BORRERO In 1996, we sent Panama the Indica gene pool GPCT-9 and the population PCT-7. That same year, Dr. Ariel E. Jaén Sánchez ofthe Faculty of Agricultura/ Sciences, Universidad de Panamá, attended the Intemational Course on Rice Recurren! Selection Breeding held at CIAT The introduced germplasm was grown and its characterization started. But, because of water shortages, irrigation was a problem. The materials suffered and the work could not be completed. Nevertheless, from each material, the earliest SO fertile plants were harvested. New samples from the recurren! populations were sent to Panama. Cropping Season 1998: The results are not yet available. 7. RECURRENT SELECTION IN VENEZUELA Eduardo GRATEROL, Marc CHÁTEL, Jaime BORRERO, and Yolima OSPINA After attending the Intemational Course on Rice Recurren! Selection Breeding, three populations (PCT-6, PCT-7, and PCT-8) and three gene pools (IRAT 1/420P, IRAT MANA, and GPCT-9) were sent to Dr. E. Graterol, for characterization under local conditions in Calabozo, Guárico State. The objective of the characterization was to select the best-adapted germplasm to start a recurren! selection program. The traits evaluated in each germplasm material were time to flowering, tillering ability, plant height, and disease tolerance (of leaf-and-neck blast, brown spot, sheath blight, and sheath rot). Two populations, PCT-6 and PCT-7, were selected as the best introduced material to be used as sources of male-sterile background to develop two new local populations, identified as PFD-1 and PFD-2. PFD-1 Male-sterile plants of PCT-6 were crossed with 5 lines: FONAIAP 1 CT9868-3-2-3-1-4P-M-1-1P IR 62140-48-3-1-2-3 CT 9509-17-3-1-1-M-1-3P-M-1 CT 1031 0-15-3-2P-4-3 PFD-2 Male-sterile plants of PCT-6 were crossed with 4 lines: CT9868-3-2-3-1-4P-M-1-1P IR 62140-48-3-1-2-3 CT 10310-15-3-lP-4-3 CT9509-17-3-1-l-M-1-3P-M-1 Cropping Season 1998 At DANAC, Venezuela and at CIAT Palmira the built-up ofthe populations PFR- 1 and PFD-2 is ongoing as planed. The use of this germplasm for the recurrent selection project ofDANAC was discussed with Eduardo Graterol. 8. RECURRENT SELECTION IN CUBA Rene Perez Polanco, Marc CHÁTEL, and Jaime BORRERO (Rene Perez polanco attended the first International course on recurrent selection held at CIAT in 1996). The results reported here are from the 1996 and 1997 cropping seasons. In 1996, the gene pool GPIRA T -1 O and the population PCT -7 were introduced to Cuba and characterized for plant type, tillering, plant height, heading time, grain type and resistance to rice blast. GPIRA T -1 O presented a better general adaptation. Nevertheless, PCT -7 is earlier witch is a characteristic of interest for the rice growing area in ·Cuba, mostly for the smallolders. By harvesting the male-sterile plants the two germplasms were recombined and seed increased. In 1997, was performed the second recombination, and 1 7 and 18 So fertile plants were selected respectively for line development. 9. RECURRENT SELECTION IN THE SOUTHERN CONE 9.1. Recurrent Selection in Argentina María Antonia MARASSI, Juan Eduardo MARASSI, M are CHATEL, and Jaime BORRERO In December 1996, we supplied the Universidad de Corrientes with the popu/ations PCT-6\0\0\0, PCT-7\0\0\0, and PCT-8\0\0\0. They were sown at the experimental field of the Company "La Arrocera Argentina", Villaguay, State of Entre Rios. They were observed and characterized. The popu/ations were multiplied by harvesting male-sterile plants. The resulting populations were identified as: PCT-6\0\0\1, PCT-7\0\0\1, and PCT-8\0\0\1. Fertile plants showing potential were selected and harvested individual/y for fine development. From the respective populations, 17, 14, and 34 SO ferti/e plants were selected and given the following identifica/ion: PCT-6> Arg-1 to 17, PCT-7> Arg-1 to 14, and PCT-8> Arg-1 to 34. For 1997 B, we planto develop a specific population by crossing 6 varieties (IRGA 417, CYPRESS, R.P.2, TAIM, Don Juan INTA, and CH 4-7) with male-sterile plants of a selected population. Argentina has a project for developing the Pampas Region, where climatic conditions are similar to those present in Chile, along the latitude with Chillan City, 400 km south of Santiago. The Universidad de la Plata has, consequently, expressed keen interest in our collaborative effort with Chile. The gene pool GPIRAT-10 and the population PQUI-1, together with Chilean and European lines, may be usefulfor the Pampas. Cropping Season 19971 1998 Line development from the populations PCT-6, 7, and 8. A total of 171 lines was selected, 65 from PCT-6, 30 from PCT-7 and 76 from PCT-8 respectively. Recurrent population multiplication. The 3 populations PCT -6, 7, and 8 Were recombined and seed increased. The Chilean popu1ation PQUI-1 was shipped to Argentina to be eva1uated in the temperate region of the "Pampas"in the province of Buenos Aires. Development of new populations with local adaptation. Population P ARG-1. This germp1asm is a new population with narrow genetic base, and corresponds to the mixture of the best male-sterile plants harvested in the se1ected progenies from fertile plants of the 3 populations PCT-6, 7, and 8. After sorne cycles of recombination it will be used as direct source of ferti1e plants fro line deve1opment. Population P ARG-2. This germplasm comes from the mixture of 50% of the population PCT -8\0\0\2 and 50o/o of P ARG-1. The result corresponds to the introduction of oriented variability in the best introduced germplasm; PCT -8. Population PARG-3. This germplasm will be set-up at CIAT by introduction of 50% of variabi1ity from 6 new lines, into the population PCT-8. This new popu1ation then will be the starting point or recurrent selection breeding in Argentina At CIAT Palmira the built-up ofthe population ARG-3 is going on as planed. 9.2. Recurrent Selection in Chile Santiago HERNAIZ, Roberto ALV ARADO, M are CHATEL, and Jaime BORRERO In 1996, we sent Chile the Japonica gene pool GPIRAT-10, which was especial/y developed by CIRAD for temperate climates. That same year, Dr. Santiago liemaiz from INIA-Qui/amapú attended the International Course on Rice Recurren! Selection Breeding held at CIAT. In 1997, the gene pool was grown for characterization and selection of the best fertile plants for /in e development. It was also used as a source of mal e sterility to bui/d up a local population by crossing ji ve Chilean /in es (Qui. 67108, Diamante, Bu/i, CINIA 609, and CINIA 606) with male-sterile plants of the gene pool. So me of the hybrid seeds were sent to CIAT, Palmira, for growing the F1 generation (Chile has only one cropping season per year). The F2 seed was shipped back to Chile in September 1997. The basic Chilean population was named PQUI-1\0\0\0. At CIAT, Palmira, during 1997 B, we will conduct the first cycle of recombination to ensure seed increase for foture use. The first cycle of recombination was identifzed as PQUI-1\0\0\1. Cropping Season 1997/1998 The Chilean population PQUI -1 was split in two parts. One sample was sown in Chillan and an other one in the northem part of the rice growing area where climatic conditions are different. The two populations identified as PQUI-1 \0\0\1 and PQUI-1\Co\1\0 were sent to CIAT Palmira for completing the second cycle of recombination. In October, 1998, the second cycle of recombination was shipped to Chile, but the seed transited through Miami and was incinerated by the plant protection service of the USA. F ortunately we ha ve not shipped all the seed produced in Palmira. Santiago Hemaiz too k the right decision to sow the first cycle of recombination in order to go-on with his project. From our part we will use the remanent see of the second recombination to perform the third cycle during the first semester of 1999. During my meeting ( during the recurrent selection course in venezuela, in September), with Santiago we plan the use of recurrent selection breeding for cold temperature. The project will start 1 during the 1999/2000 cropping season in Chile. 9.3. Recurrent Selection in Uruguay Fernando PÉREZ DE VIDA, Marc CHATEL, and Jaime BORRERO In 1996, we sent to Uruguay the Japonica gene pool GPIRAT-10. That same year, Dr. Fernando Pérez de Vida from IN/A-Treinta y Trés attended the International Course on Rice Recurrent Selection Breeding held at CIAT In 1997, the gene pool was grown for characterization and selection of the best fertile plants for /in e development. It was also used as a source of mal e sterility to build up a local population by crossing selected Uruguayan fines with male-steri/e plants ofGPIRAT-10. Cropping Season 1998 During the 1997 cropping season, Fernando Perez de Vida crosses different lines with sterile plants from the GPIRA T -1 O gene pool. The hybrid seed was shipped to CIA T Palmira to grow the F l. Three (3) populations will be developped: PURG-1 comes from the introduction of 17 lines with short grain quality into GPIRAT-10. The objective is to develop a local population for short grain quality, witch represents a new target export market for Uruguay. PURG-2 comes from the introduction of 60 long grain lines into the gene pool GPIRAT-10. The third population identified as PURG-3 , has a broad genetic base and is the result of the physical mixture of the two previous populations with male-sterile plants selected in the best progenies derived from the original gene pool GPIRAT-10. 9.4. Recurrent Selection by CIRAD, in Rio Grande do Sul, Brazil James TAILLEBOIS and Marc CHÁTEL (James TAILLEBOIS is the person that developed the first broad genetic base germplasm and initiated the use of recurrent selection, in the framework of a collaborative project on hybrid rice between CIRAD and EMBRAPA Arroz e Feijiio, in Goiania, Brazil). In 1997, we sent to the CIRAD/G4I collaborative project, the populations IRA T MANA and PCT -6 for evaluation in Pelotas, Rio Grande do Sul, Brazil. Cropping season 1998 The two germplasms were evaluated under local condition. - IRAT MANA is not adapted to the subtropical condition showing long growing cycle. His main quality of the genotypes present in this population is grain shape and quality (long slender and translucent grains ). The segregation ratio between fertile and male-sterile plants was 50% for each genotype. 26 male-sterile plants were harvested to develop a narrow genetic base population identified as OOEP. During the next cropping season the will be recombined, and a new round of selection of mal e-fertile plants performed. - PCT-6 also presented a great number of genotypes showing late maturity. But about 20% of the genotypes presented a cycle inferior or equal to the local commercial variety BR-IRGA 417. Direct use of this population for line development is possible. 39 male-sterile plants showing good earliness and adapted phenotypes were harvested to develop a new population identified as OONP. During the next cropping season these genotypes will be recombined and crossed with A lines from the hybrid program, to evaluate the general combining ability (GCA) and restoration ability of the population. James is interested in receiving the japonica upland population PCT-11 to start sorne studies onjaponica hybrid rice. 10. MAINTAINING GERMPLASM BRED BY RECURRENT SELECTION Because we manage the catalogue for rice germplasm bred by recurrent selection, we also have the responsibility to ensure the presence of sufficient seed in the germplasm bank. Because of the sufficient disposability of seed, no multiplication was done this year. 11. DISTRIBUTING LOWLAND-RICE GERMPLASM BRED BY RECURRENTSELECTION Since 1995, we started to release recurrent selection populations and gene pools to NARS in Latin America and in countries of W est Africa, Asia, and Europe 12. REGISTERING NEW POPULATIONS In 1998, two CIRAD populations, developed for lowlands, by Dr. James Taillebois, for yield potential and grain quality were proposed for registration in the Recurrent Selection Catalogue managed by our project. The populations were built up in Brazil as part of the collaborative proj ect between CIARD and G4 l. They were registered as PCIRAD-23 and PCIRAD-24. 13. LINE DEVELOPMENT THROUGH ANTHER CULTURE Zaida LENTINI, Marc CILiTEL, James GIBBONS, and Yolima OSPINA In 1994, we introduced the population IRA T -CT from French Guiana. This population comes from the enhancement of the Indica gene pool GPCNA-18 for anther culture response. Cycles One cycle of selection-recombination for anther culture response was previously made in Brazil at EMBRAPA Arroz e Feijao. This gave rise to the population identified as IRAT-CT. From 1995, the anther culture laboratory at CIAT processed the population IRAT-CT and R2 lines were developed. Cropping Season 1997 The R2 lines were evaluated by FLAR at the Santa Rosa Experiment Station and five were selected. Cropping Season 1998: The 5 lines selected by FLAR were evaluated. 14. RECURRENT SELECTION AND ANTHER CULTURE FOR CIDLE Santiago HERNAIZ, Marc CHATEL, Jaime BORRERO, and Adriana MORA To fasten the development of fixed lines for Chile where only one cropping season is possible, we decided to process the two Chilean populations with two recurrent selection cycles through anther culture. The populations will be sown in Palmira and plants processed at the CIA T laboratory. l j \ l j t l 1 l ! ¡ r 1 l 1 i 1 1 1 ~· We hope to ship the DH lines to Chile for sowing during the 1999/2000 cropping season. 15. CONVENTIONAL BREEDING AND ANTHER CULTURE FOR ROMANIA George ALIONTE, Marc CHATEL, Jaime BORRERO, and Adriana MORA In the framework of the collaboration CIRAD-CA rice project has with the Romanian institution FUNDULEA, two crosses, from Romanía, designed for cold tolerance, grain quality, and yield potential are being processed by the CIA T anther culture laboratory. One hundred (100) double haploid (DH) lines by cross will be produced, observed and dispatched back to FUNDULEA. We will keep a sample of each DH line for seed increase. These lines will be sent to Chile, Argentina, and Uruguay. They can a1so be useful as parents for the hillside upland rice breeding project. Cropping season 1998 Dr G. Alionte solicited our project for the processing by anther culture, two very promising Romanian crosses; OLTENITSA 1 RUBINO and CRISTAL 1 L 203. The work is going-on as p1aned. We hope to have about 100 DH lines from each cross. The DH lines will be shipped to Romania, and we will keep sorne seed for shipping to our partners in Chile and Argentina. TABLES Table l. Soil analysis ofthe experimental site (Lote Loma S) at" la libertad" Experimental Station, Colombia, 1998 cropping season. p Al No Depth o .. M Brayll pH Al Ca Mg K C.I.E B Zn Mn Cu Fe Sat (cm) (%) (ppm) Meq./IOOgr ppm (%) 1 0-20 3.2 8.1 4.7 2.60 0 .59 0.25 O.ll 3.55 0.26 0.55 12.64 0.68 25.6 73.24 20-40 2.4 1.6 4.7 2.60 0.29 0.11 0.06 3.06 0.24 0.28 9.19 0 .66 14.0 &4.97 2 0-20 3.7 8.3 4 .8 2 .39 0.52 0.22 0.12 3.25 0.31 0.48 10.20 0.45 18.6 73.54 20-40 2.8 3.6 4 .8 2.29 0 .29 0.12 0.05 2.75 0.21 0.26 6.40 0.42 11.5 83.27 Table 2. Climatíc characteristics ofthe 1998 cropping season at " La Libertad" Experimental Statio~ Colombia. Characteristcs April May June July Aug. Sep. Total Rainfall (mm) 425.3 365.5 471.9 369.3 138.6 122.5 1893. 1 Days of rain (No.) 17 18 21 27 19 19 121 Temperature (maximwn oC) 31.4 30.0 28.6 29.0 30.4 31.0 30.1 Temperature (minimwn °C) 23.2 22.6 21.9 21.6 21.6 21.6 22.1 Relative hwnidity (%) 84 85 86 87 84 82 85 Table 3. Evaluation of S2 lines from the populations PCT-4\PHB\1\1,PHB\1 ; PCT-5\PHB\1\0,PHB\1 and PCT-A\PHB\1\0,PHB\1 . La Libertad experimental station, 1998A Nbr. Field Nbr. Origin Pedigree 1998A 19978 1 S82.aXl1 2 S82(XX)2 3 S82CXXJ3 4 S82CXX)4 5 S82CXX:6 6 S82(XX)6 7 $82(007 8 se~ 9 se~ 10 S820010 11 $820011 12 $820012 13 S820013 14 S820014 15 $820015 16 S820016 17 S820017 18 $820018 19 $820019 20 S820020 21 S820021 22 S820022 23 $820023 24 S820024 25 $820025 26 $820026 27 S820027 28 5820028 29 S820029 3) 582CXB:l 31 $820031 32 S820032 33 S820033 34 5820034 36 S820035 36 5820036 37 $820037 38 S820038 33 S82CXm 40 S820040 41 S820041 42 S820042 43 S820043 44 S820044 45 S820045 46 S820046 47 5820047 48 S820048 49 5820049 50 S82al50 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 16 19 20 21 22 23 24 25 26 27 28 29 3) 31 32 33 34 36 36 37 38 33 40 41 42 43 44 45 46 47 48 49 50 PCT -4\PHB\1 \1 ,PHB\1 >46-M PCT -4\PHB\1\1 ,PHB\1 >120-M PCT -4\PHB\1\1 ,PHB\1 >33)-M PCT -4\PHB\1\1 ,PHB\1 >:D>M PCT -4\PHB\1\1 ,PHB\1 >384-M PCT -4\PHB\1\1,PHB\1 >412-M PCT -4\PHB\1\1 ,PHB\1 >491-M PCT -4\PHB\1\1,PHB\1 >566-M PCT -4\PHB\1\1,PHB\1 >61 ~ PCT -4\PHB\1\t,PHB\1 >649-M PCT -4\PHB\1\1,PHB\1 >682-M PCT -4\PHB\1\t ,PHB\1 > 762-M PCT -4\PHB\1\t ,PHB\1 ~ PCT -4\PHB\1\t,PHB\1 >897-M PCT -4\PHB\1\1,PHB\1 ~ PCT -4\PHB\1\1,PHB\1 >968-M PCT -4\PHB\1\1 ,PHB\1 >1035-M PCT -4\PHB\1\1,PHB\1 >1039-M PCT -4\PHB\1\t,PHB\1 >1176-M PCT -4\PHB\1 \1 ,PHB\1 >1213-M PCT -4\PHB\1\t,PHB\1 >1232-M PCT -4\PHB\1\t ,PHB\1>1244-M PCT -4\PHB\1\1,PHB\1 >1262-M PCT -4\PHB\1\1,PHB\1 >1295-M PCT -4\PHB\1\t ,PHB\1 >1388-M PCT -4\PHB\1\t,PHB\1 >14D-M PCT -4\PHB\1\t,PHB\1 >1484-M PCT -4\PHB\1\t,PHB\1 >1534-M PCT -4\PHB\1\1,PHB\1 >1537-M PCT -4\PHB\1\1,PHB\1 >1563-M PCT -4\PHB\1\1 ,PHB\1 >1~ PCT -4\PHB\1 \1 ,PHB\1 >1 007-M PCT -4\PHB\1\1 ,PHB\1 >1621-M PCT -4\PHB\1\1 ,PHB\1 >1733-M PCT -4\PHB\1\1,PHB\1 >1737-M PCT -4\PHB\1\1,PHB\1 >1700-M PCT -4\PHB\1\t,PHB\1 >1 774-M PCT -4\PHB\1\1,PHB\1>1776-M PCT -4\PHB\1\1 ,PHB\1 >1866-M PCT -4\PHB\1\1 ,PHB\1 >187&M PCT -4\PHB\1\1,PHB\1 >1970-M PCT -5\PHB\1'D,PHB\1>1 7&M PCT-5\PHB\1'D,PHB\1 >201-M PCT -5\PHB\1'D,PHB\1 ~M PCT -5\PHB\1 'D,PHB\1 > 793-M PCT -5\PHB\1'D,PHB\1 >1014-M PCT -5\PHB\1'D,PHB\1 >1ce6-M PCT -5\PHB\1'D,PHB\1 >1125-M PCT -5\PHB\1'D,PHB\1 >1128-M PCT -5\PHB\1'D,PHB\1 >1150-M Vg Bl Bl Fl LSc BS GD NBI 1 2 50% 5 4 3 62 7 2 3 71 7 2 2 65 7 3 3 70 7 3 2 86 5 3 3 74 5 4 4 82 5 3 2 71 5 2 3 71 3 2 2 79 9 2 84 7 2 1 81 7 1 2 74 7 2 3 92 7 2 85 5 2 77 5 2 2 93 5 4 3 77 7 2 2 82 7 3 2 84 5 2 2 75 5 2 2 74 9 3 2 86 7 3 3 83 5 3 2 77 7 2 2 82 7 5 5 70 3 2 2 67 5 1 1 71 5 2 3 82 3 3 3 74 5 2 2 81 3 2 2 89 5 3 2 67 7 5 5 74 7 3 3 82 5 3 3 87 7 2 2 76 5 3 2 11 5 2 2 82 7 1 2 77 5 3 3 89 3 4 3 85 7 3 3 76 5 3 3 72 5 2 1 73 7 3 4 85 5 4 4 61 5 4 4 77 7 2 64 3 1 3 3 1 1 3 3 3 1 3 3 1 3 3 1 1 3 3 1 3 1 1 1 1 3 3 1 3 3 1 3 1 3 1 1 1 3 1 3 1 1 3 1 1 1 3 3 1 3 3 3 1 1 1 3 3 3 1 3 1 1 3 3 3 1 3 3 1 3 3 5 3 3 3 1 1 3 3 5 1 3 3 1 3 3 1 3 3 1 1 3 3 3 1 3 5 1 1 3 1 3 3 3 3 3 5 3 3 51 5820051 51 PCT -5\PHB\1'i>,PHB\1 >1162-M 5 2 2 70 5 3 52 5820052 52 PCT -5\PHB\1'i>,PHB\1 >1437-M 5 3 3 74 5 3 53 5820053 53 PCT -5\PHB\t'(),PHB\1 >1fS4-M 3 4 4 88 3 1 54 5820054 54 PCT -5\PHB\1'i>,PHB\1 >1996-M 5 3 2 78 5 5 3 55 582CX155 55 PCT-A\PHB\1'i>,PHB\1>53-M 5 3 3 75 3 3 56 582aa5 56 PCT-A\PHB\1'i>,PHB\1>3D-M 5 3 2 85 5 3 3 57 5820057 57 PCT-A\PHB\1'i>,PHB\1 >368-M 5 80 3 1 58 5820058 58 PCT-A\PHB\1'i>,PHB\1 >478-M 5 3 2 83 3 1 59 5820059 59 PCT-A\PHB\1'i>,PHB\1 >ffi4-M 7 3 3 69 3 1 ro 5820000 ro PCT -A\PHB\1'i>,PHB\1 >811-M 7 1 1 77 5 3 61 5820061 61 PCT-A\PHB\1'i>,PHB\1 >817-M 5 2 2 68 3 3 62 S820062 62 PCT -A\PHB\1 'i>,PHB\1 ~ 7 2 2 70 3 3 63 5820063 63 PCT-A\PHB\1'i>,PHB\1>968-M 5 3 3 79 3 1 64 S820064 64 PCT -A\PHB\1'i>,PHB\1 >1396-M 3 2 2 74 5 3 3 66 5820066 66 PCT-A\PHB\1'i>,PHB\1>151~M 5 2 2 80 3 1 66 S820066 66 PCT -A\PHB\1 'i>,PHB\1 >1768-M 5 3 2 76 3 3 Vg = vigor; 81 1 = leaf b1ast ; Bl 2 = leaf blast ; Fl = flowering; LSc = leaf scald; BS = brown spot; NBI = neck blast; Gd = grain discoloration. Table 4. S2 lines selected in the populations PCT-4\PHB\1\1 ,PHB\1 ; PCT-5\PHB\1\0,PHB\1; and PCT-A\PHB\1\0,PHB\1 La Libertad experimental station, 1998A Nbr. Field Nbr. Origin Pedigree Vg Bl 81 Fl LSc BS GD NBI 1998A 19978 1 2 50% 1 $8200)1 1 PCT -4\PHB\1\1 ,PHB\1 >46-M 5 4 3 82 2 $8200)2 2 PCT -4\PHB\1\1,PHB\1 >120-M 7 2 3 71 3 $82(003 3 PCT -4\PHB\1\1,PHB\1 >~M 7 2 2 re 1 1 4 5820028 28 PCT -4\PHB\1 \1,PHB\1 >1534-M 3 2 2 67 3 1 3 5 $820029 29 PCT -4\PHB\1\1 ,PHB\1 >1537-M 5 1 71 1 3 6 $820038 38 PCT -4\PHB\1\1 ,PHB\1 >1776-M 7 2 2 76 3 7 $820052 52 PCT -5\PHB\i'D,PHB\1>1437-M 5 3 3 74 5 3 8 5820062 62 PCT-A\PHB\1VJ,PHB\1 >853-M 7 2 2 70 3 3 Vg = vigor; 811 = leaf blast ; Bl 2 = Jeaf blast ; Fl = flowering;' LSc = Jeaf scald; BS = brown spot; NBI = neck blast; Gd = grain discoloration. Table 5. S2 line evaluation of the population PCT-4\SA\1\1 (Augmented Designs of Federer) La Libertad experimental station, 1998A Nbr. Field Nbr. Origin Pedigree 1998A 19978 1 890001 2 890002 3 890003 4 890004 5 890005 6 890006 7 890007 8 890008 890009 9 890010 10 890011 11 890012 12 890013 13 890014 14 890015 890016 15 890017 890018 16 890019 17 890020 18 890021 19 890022 20 890023 21 890024 890025 22 890026 23 890027 24 890028 25 890029 26 890030 27 890031 28 890032 29 890033 30 . 890034 31 890035 32 890036 33 890037 34 890038 35 890039 890040 36 890041 37 890042 890043 38 890044 39 890045 40 890046 41 890047 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 PCT -4\SA\ 1\1 >33-M PCT -4\SA\ 1\1 >45-M PCT -4 \SA \1 \1 >95-M PCT -4\SA\1\1 >109-M PCT-4\SA\1\1>115-M PCT -4\SA\1\1>126-M PCT -4\SA\1\1>147-M PCT -4\SA\1\1>150-M O.S.6 PCT -4\SA\1\1>153-M PCT -4\SA\1\1 >162-M PCT -4\SA\1\1>171-M PCT-4\SA\1\1>179-M PCT-4\SA\1\1>188-M PCT-4\SA\1\1>193-M O.S.10 PCT -4\SA\1\1>195-M C409 PCT -4\SA\1\1>223-M PCT -4\SA\1\1>230-M PCT -4\SA\1\1 >236-M PCT -4\SA\1\1>241-M PCT -4\SA\1\1>249-M PCT -4\SA\1\1>252-M C409 PCT -4\SA\1\1>260-M PCT -4\SA\1\1>261-M PCT -4\SA\ 1\1 >279-M PCT -4\SA\1\1>305-M PCT -4\SA\1\1 >306-M PCT-4\SA\1\1 >311-M PCT -4\SA\1\1>312-M PCT -4\SA\1\1>322-M PCT -4\SA\ 1\1 >341-M PCT -4\SA\1\1>379-M PCT -4\SA\1\1>390-M PCT -4\SA\1\1>402-M PCT -4\SA\1\1 >428-M PCT -4\SA\1\1 >437-M O.S.10 PCT -4\SA\ 1\1 >440-M PCT -4\SA\1\1>442-M O.S.6 PCT-4\SA\1\1>444-M PCT -4 \SA \1 \1 >446-M PCT -4\SA\1\1 >500-M PCT-4\SA\1\1>503-M Vg Ht Bl BJ Fl LSc BS GD NBI 1 2 50% 7 105 3 3 7 104 3 3 7 100 2 2 7 104 2 2 7 96 3 2 7 99 1 1 7 100 1 7 97 1 1 5 92 3 3 5 103 1 2 5 116 1 2 5 115 1 2 3 116 1 3 3 104 1 2 3 118 1 2 5 111 3 3 7 88 5 98 1 5 105 5 93 1 1 5 96 1 1 5 98 2 5 88 1 7 98 2 3 7 97 1 1 7 104 2 3 7 102 2 3 7 106 1 2 7 108 1 3 7 108 1 3 5 106 2 4 5 87 1 3 7 86 1 3 7 93 1 4 5 90 1 3 5 88 1 3 5 88 3 5 102 3 5 96 1 3 5 106 2 3 5 92 1 3 5 90 2 3 5 114 4 4 7 96 3 4 7 99 3 7 101 3 5 94 1 2 81 3 1 1 1 79 3 1 1 70 3 1 1 1 71 3 1 1 1 75 3 1 1 1 70 3 1 1 1 71 3 1 1 75 3 1 1 88 3 3 3 1 87 3 1 1 1 87 3 1 1 1 86 3 1 1 87 3 1 1 86 3 1 1 84 5 1 1 1 91 5 1 1 1 82 3 1 1 1 71 3 3 1 81 3 1 1 83 3 1 1 83 3 1 1 81 3 1 1 82 3 1 1 1 70 3 1 1 1 77 1 1 1 1 73 1 1 1 1 71 1 1 71 1 1 1 74 1 1 1 1 70 3 1 1 75 3 1 1 1 65 3 1 1 1 70 3 1 1 1 67 3 1 1 63 3 1 1 65 3 1 1 85 3 1 1 83 3 1 1 1 82 3 3 93 5 3 1 1 66 3 3 1 65 3 3 3 87 3 1 1 1 77 3 3 1 1 70 3 1 1 68 3 3 1 68 3 1 42 890048 43 890049 44 890050 45 890051 890052 46 890053 47 890054 48 890055 49 890056 890057 50 890058 890059 51 890060 52 890061 53 890062 54 890063 55 890064 56 890065 57 890066 58 890067 59 890068 60 890069 61 890070 890071 62 890072 63 890073 890074 64 890075 65 890076 66 890077 67 890078 890079 68 890080 69 890081 70 890082 71 890083 72 890084 73 890085 74 890086 75 890087 76 890088 77 890089 78 890090 79 890091 80 890092 81 890093 82 890094 83 890095 890096 84 890097 85 890098 86 890099 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 PCT -4\SA\ 1\1 >516-M PCT -4\SA\ 1\1 >540-M PCT -4\SA\1\1>542-M PCT -4\SA\1\1>571-M O.S.6 PCT -4\SA\1\1>573-M PCT -4\SA\ 1\1 >592-M PCT -4\SA\1\1>594-M PCT -4\SA\1\1>595-M O.S.10 PCT -4\SA\ 1\1 >631 -M C409 PCT -4\SA\1\1>632-M PCT -4\SA\1\1>659-M PCT -4\SA\1\1>669-M PCT -4\SA\1\1>674-M PCT -4\SA\1\1>701-M PCT -4\SA\1\1>721-M PCT -4\SA\1\1>722-M PCT -4\SA\1\1>723-M PCT -4\SA\1\1>763-M PCT -4\SA\1\1>781-M PCT -4\SA\1\1>783-M O.S.6 PCT -4\SA\1\1>813-M PCT -4\SA\1\1>881-M O.S.10 PCT -4\SA\1\1>895-M 5 94 2 2 5 92 2 2 5 86 2 5 87 1 2 5 107 3 4 5 84 2 5 89 1 2 5 102 2 2 5 93 2 2 3 105 3 3 3 102 1 2 5 99 1 1 5 104 2 2 5 100 2 4 5 96 2 3 5 99 3 3 5 93 1 2 7 94 4 5 7 85 4 4 7 85 4 4 7 84 3 3 7 86 2 2 7 79 3 3 5 112 4 4 7 89 1 2 3 93 1 1 3 103 2 3 3 103 1 PCT -4\SA\1\1>910-M 3 101 2 3 PCT -4\SA\1\1>911-M 3 98 1 1 PCT-4\SA\1\1>948-M 5 105 1 1 e 409 5 96 1 1 PCT -4\SA\1\1>954-M 5 96 2 3 PCT -4\SA\1\1>957-M 5 90 2 2 PCT -4\SA\1\1 >975-M 5 95 2 2 PCT -4\SA\1\1>982-M 5 96 1 3 PCT-4\SA\1\1>1013-M 5 86 1 2 PCT-4\SA\1\1>1019-M 5 84 1 3 PCT-4\SA\1\1>1034-M 5 94 1 3 PCT -4\SA\1\1>1036-M 5 102 1 3 PCT -4\SA\1\1>1044-M 5 96 3 3 PCT-4\SA\1\1>1047-M 5 97 3 4 PCT-4\SA\1\1>1048-M 5 96 2 3 PCT-4\SA\1\1>1108-M 3 100 2 2 PCT-4\SA\1\1>1116-M 5 95 2 2 PCT -4\SA\1\1>1127-M 5 91 1 1 PCT-4\SA\1\1>1128-M 3 101 2 3 PCT -4\SA\1\1>1135-M 3 106 3 3 O.S.6 3 95 4 4 PCT-4\SA\1\1>1138-M 5 89 1 1 PCT-4\SA\1\1>1145-M 5 84 2 3 PCT-4\SA\1\1>1155-M 5 88 1 2 71 3 1 1 1 68 3 1 1 1 66 3 1 1 1 71 3 3 1 1 86 3 1 1 1 70 3 1 1 1 70 3 1 1 75 3 1 3 1 68 3 1 1 89 3 3 1 74 3 1 1 1 70 3 1 1 1 75 5 1 1 1 75 5 1 1 1 75 5 1 1 1 70 5 1 1 1 75 5 1 1 1 75 3 1 1 1 71 3 1 1 68 3 1 1 3 71 1 1 1 1 75 1 1 1 1 66 1 1 1 3 86 5 3 3 1 73 1 1 1 71 3 1 3 1 89 5 3 1 1 74 3 1 1 1 75 3 1 1 1 70 3 1 1 74 3 1 1 1 70 5 1 1 70 3 1 1 1 75 3 1 1 1 71 3 1 1 1 73 3 1 1 1 79 3 1 1 1 75 3 1 1 1 70 3 1 1 1 75 3 1 1 78 3 1 1 1 79 5 1 1 1 82 3 1 1 1 75 3 1 1 1 79 3 1 1 1 75 3 1 1 1 66 5 1 5 71 5 1 5 1 88 5 3 3 1 71 5 1 1 1 69 5 1 1 1 74 5 1 5 ; .. ~ 890100 87 890101 890102 88 890103 89 890104 90 890105 91 890106 92 890107 93 890108 94 890109 95 890110 96 890111 97 890112 98 890113 99 890114 100 890115 101 890116 102 890117 890118 103 890119 104 890120 105 890121 106 890122 107 890123 108 890124 109 890125 110 890126 890127 11 890128 112 890129 890130 113 890131 114 890132 115 890133 116 890134 117 890135 118 890136 119 890137 890138 120 890139 121 890140 122 890141 890142 123 890143 124 890144 125 890145 126 890146 127 890147 128 890148 129 890149 130 890150 131 890151 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 O.S.10 PCT -4\SA\1\1>1156-M C409 PCT -4\SA\1\1>1199-M PCT -4\SA\1\1>1201-M PCT -4\SA\1\1>1206-M PCT -4\SA\1\1 >1231-M PCT -4\SA\1\1>1236-M PCT -4\SA\1\1>1241-M PCT -4\SA\ 1\1 >1259-M PCT -4\SA\1\1>1260-M PCT -4\SA\1\1>1270-M PCT -4\SA\1\1>1272-M PCT -4\SA\1\1>1293-M PCT -4\SA\1 \1>1302-M PCT -4\SA\1\1>1320-M PCT -4\SA\1\1>1337-M PCT -4\SA\1\1>1344-M O.S.6 PCT -4\SA\1\1>1358-M PCT -4\SA\1\1>1376-M PCT -4\SA\1\1>1377-M PCT -4\SA\1\1>1389-M PCT -4\SA\1\1>1392-M PCT -4\SA\1\1 >1414-M PCT -4\SA\1\1>1443-M PCT -4\SA\1\1>1472-M O.S.10 PCT -4\SA\1\1>1475-M PCT -4\SA\1\1>1479-M C409 PCT -4\SA\1\1>1486-M PCT -4\SA\1\1>1512-M PCT -4\SA\ 1\1 >1538-M PCT -4\SA\ 1\1 >1549-M PCT -4\SA\1\1 >1559-M PCT -4\SA\1\1>1566-M PCT -4\SA\1\1>1576-M O.S.6 PCT -4\SA\1\1>1584-M PCT -4\SA\1\1>1632-M PCT -4\SA\1\1>1642-M O.S.10 3 5 5 5 5 7 7 7 7 7 7 7 7 7 7 7 7 5 3 7 7 5 5 5 5 5 5 5 94 2 3 92 2 2 96 2 2 96 2 95 3 95 1 2 98 1 2 95 1 2 94 3 3 92 2 2 89 2 3 93 2 4 92 1 97 1 1 96 1 97 1 95 1 96 2 95 4 4 95 2 1 96 2 1 78 3 3 86 4 4 85 4 4 89 4 4 84 4 4 89 4 5 98 3 4 5 102 2 2 3 90 2 2 3 93 2 1 3 102 2 2 5 102 4 3 5 77 4 4 7 92 2 2 7 99 2 2 5 98 2 2 5 98 2 2 3 93 4 4 5 106 2 3 7 100 1 2 5 96 2 2 3 95 3 4 PCT-4\SA\1\1>1666-M 5 88 1 2 PCT-4\SA\1\1>1667-M 5 92 2 2 PCT-4\SA\1\1>1689-M 5 89 2 2 PCT -4\SA\1 \1>1691-M 5 86 3 3 PCT -4\SA\1\1>1701-M 5 93 2 2 PCT-4\SA\1\1>1702-M 5 91 2 2 PCT-4\SA\1\1>1709-M 7 103 2 1 PCT-4\SA\1\1>1731-M 5 102 2 PCT-4\SA\1\1>1732-M 7 102 2 94 5 3 3 68 5 1 1 1 76 5 1 1 71 5 1 1 1 71 5 1 1 70 3 1 1 1 77 3 1 1 71 3 1 1 1 71 3 1 1 1 71 3 1 1 67 3 1 1 1 70 3 1 1 1 71 3 1 1 77 3 3 1 75 3 3 1 1 73 3 1 1 1 77 3 3 1 75 3 3 1 1 88 5 3 3 1 76 3 1 1 1 74 3 3 1 1 85 3 1 84 3 1 1 73 3 1 1 1 70 3 1 1 3 84 3 1 3 88 3 1 1 3 91 5 3 3 1 75 5 1 1 1 73 3 1 1 71 3 1 1 74 3 75 3 1 81 3 1 1 3 76 3 1 1 71 5 1 1 74 5 1 1 74 3 1 1 1 87 5 3 3 1 72 5 1 74 3 3 1 71 3 1 1 1 86 5 3 1 1 71 3 1 1 1 71 3 1 1 1 71 3 1 1 75 3 1 1 1 71 3 1 1 72 3 1 1 1 63 3 1 1 3 62 3 1 1 3 62 3 1 1 3 132 890152 152 PCT-4\SA\1\1>1740-M 7 100 2 1 63 3 1 1 3 133 890153 153 PCT -4\SA\1\1 >1776-M 7 104 2 1 62 3 1 3 890154 154 C409 7 100 2 1 75 5 1 1 134 890155 155 PCT-4\SA\1\1 >1792-M 7 100 2 1 62 3 1 1 3 135 890156 156 PCT -4\SA\1\1>1802-M 7 100 2 1 70 3 1 1 1 136 890157 157 PCT-4\SA\1\1>1837-M 7 104 2 1 71 3 1 1 137 890158 158 PCT-4\SA\1\1>1843-M 7 102 2 1 70 3 3 1 1 138 890159 159 PCT -4\SA\1\1>1856-M 7 92 2 2 71 3 3 1 1 890160 160 O.S.6 5 96 5 4 88 3 1 139 890161 161 PCT -4\SA\1\1 >1861-M 5 88 2 1 70 5 3 1 140 890162 162 PCT -4\SA\1\1>1875-M 5 86 2 1 68 5 3 1 1 890163 163 O.S.10 5 99 2 2 93 5 3 1 1 141 890164 164 PCT -4\SA\1\1>1878-M 5 98 1 1 74 5 1 1 1 142 890165 165 PCT -4\SA\1\ 1 >1880-M 5 100 1 2 71 5 1 1 1 143 890166 166 PCT -4\SA\1\1>1881-M 5 99 1 1 74 3 1 1 144 890167 167 PCT -4\SA\ 1\1 >1896-M 5 102 2 2 74 3 1 1 1 145 890168 168 PCT -4\SA\1\1>1915-M 5 110 2 75 5 1 1 1 146 890169 169 PCT-4\SA\1\1>1917-M 7 104 2 1 82 3 1 1 1 147 890170 170 PCT -4\SA\1\1>1919-M 7 87 1 2 73 3 1 1 890171 171 C409 5 96 2 1 71 3 1 1 1 148 890172 172 PCT -4\SA\1 \1>1921-M 7 90 2 2 75 3 3 1 149 890173 173 PCT-4\SA\1\1>1925-M 7 89 2 1 75 3 1 1 1 150 890174 174 PCT -4\SA\1 \1>1928-M 7 88 1 2 71 3 1 1 1 151 890175 175 PCT -4\SA\ 1\1 >1947 -M 7 93 2 2 75 3 1 1 1 152 890176 176 PCT -4\SA\ 1\1 >1948-M 7 87 4 3 71 3 1 1 Vg =vigor; 81 1 = leaf blast ; 81 2 = leaf blast ; Fl = flowering; LSc ::: leaf scald; 8S = brown spot; N81 = neck blast; Gd = grain discoloration; Ht = plant heighl Table 6. S2 lines from the population PCT -4\SA\1\1, selected in the evaluation trial ( Augmented Design of Federer) La Libertad experimental station, 1998A Nbr.. Field Nbr. Origln Pedlgree 1998A 19978 1 890002 2 PCT-4\SA\1\1>45-M 2 890003 3 PCT-4\SA\1\1>95-M 3 890004 4 PCT-4\SA\1\1>109-M 4 890006 6 PCT -4\SA\1\1>126-M 5 890007 7 PCT -4\SA\1\1>147-M 6 890008 8 PCT-4\SA\1\1>150-M 7 890011 11 PCT-4\SA\1\1>162-M 8 890014 14 PCT -4\SA\1\1>188-M 9 890015 15 PCT-4\SA\1\1>193-M 10 890017 17 PCT-4\SA\1\1>195-M 11 890019 19 PCT-4\SA\1\1>223-M 12 890020 20 PCT -4\SA\1\1>230-M 13 890021 21 PCT-4\SA\1\1>236-M 14 890026 15 890027 16 890028 17 890029 18 890030 19 890034 20 890041 21 890045 22 890046 23 890047 24 890048 25 890049 26 890053 27 890058 28 890060 29 890062 30 890065 31 890066 32 890068 33 890072 34 890073 35 890075 36 890076 37 890078 38 890082 39 890083 40 890084 41 890087 42 890088 43 890091 44 890092 45 890093 46 890101 47 890103 48 890105 49 890107 50 890108 26 PCT -4\SA\1\1>260-M 27 PCT -4\SA\1\1>261-M 28 PCT-4\SA\1\1>279-M 29 PCT -4\SA\1\1>305-M 30 PCT -4\SA\1\1>306-M 34 PCT-4\SA\1\1>341-M 41 PCT-4\SA\1\1>440-M 45 PCT-4\SA\1\1>446-M 46 PCT -4\SA\1\1>500-M 47 PCT -4\SA\1\1>503-M 48 PCT-4\SA\1\1>516-M 49 PCT -4\SA\1\1>540-M 53 PCT-4\SA\1\1>573-M 58 PCT-4\SA\1\1>631-M 60 PCT -4\SA\1\1>632-M 62 PCT-4\SA\1\1>669-M 65 PCT-4\SA\1\1>721-M 66 PCT -4\SA\1\1>722-M 68 PCT-4\SA\1\1>763-M 72 PCT-4\SA\1\1>813-M 73 PCT-4\SA\1\1>881-M 75 PCT -4\SA\1\1>895-M 76 PCT -4\SA\1\1>910-M 78 PCT-4\SA\1\1>948-M 82 PCT -4\SA\1\1 >975-M 83 PCT -4\SA\1\1>982-M 84 PCT -4\SA\1\1>1013-M 87 PCT-4\SA\1 \1>1036-M 88 PCT-4\SA\1\1>1044-M 91 PCT -4\SA\1\1 >1108-M 92 PCT-4\SA\1\1>1116-M 93 PCT -4\SA\1\ 1>1127-M 101 PCT -4\SA\1\1 >1156-M 103 PCT-4\SA\1\1>1199-M 105 PCT-4\SA\1\1>1206-M 107 PCT -4\SA\1\1>1236-M 108 PCT -4\SA\1\1>1241-M Vg Ht Bl Bl Fl LSc BS GD NBI 1 2 so•¡. 7 104 7 100 7 104 7 99 7 100 7 97 5 116 3 104 3 118 7 88 5 105 5 93 5 96 7 104 7 102 7 106 7 108 7 108 · 7 93 5 92 7 99 7 101 5 94 5 94 5 92 5 84 3 102 5 104 5 96 7 94 7 85 7 84 7 89 3 93 3 103 3 101 5 105 5 95 5 96 3 3 79 2 2 70 2 2 71 70 1 71 1 1 75 1 2 87 1 2 86 3 3 3 3 3 3 3 3 1 1 1 1 1 1 1 1 2 84 5 1 1 1 1 1 2 3 2 3 2 3 3 1 4 3 1 3 1 3 2 2 2 2 2 2 1 2 2 2 2 3 4 5 4 4 3 3 1 2 1 1 1 1 2 3 1 2 2 3 82 81 83 83 73 71 71 74 70 67 66 70 68 68 71 68 70 74 75 75 75 71 71 73 71 74 75 74 71 73 3 3 3 3 1 1 1 3 3 3 3 3 3 3 3 3 3 5 5 3 3 1 1 3 3 3 3 3 3 1 1 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 5 86 1 2 79 3 1 5 102 1 3 75 3 1 1 5 96 3 3 78 3 1 1 1 3 100 2 2 75 3 1 5 95 2 2 79 3 1 5 91 1 75 3 5 92 2 2 68 5 96 1 2 71 7 95 2 70 7 95 1 2 71 7 94 3 3 71 5 5 1 3 1 3 1 3 1 1 1 1 1 1 1 1 1 51 890110 110 PCT -4\SA\1\1>1260-M 7 89 2 3 67 3 1 1 1 52 890111 111 PCT -4\SA\1\1>1270-M 7 93 2 4 70 3 1 1 1 53 890112 112 PCT -4\SA\1\1>1272-M 7 92 1 71 3 1 1 1 54 890128 128 PCT -4\SA\1\1>1475-M 5 102 2 2 75 5 1 1 55 890129 129 PCT -4\SA\1\1>1479-M 3 90 2 2 73 3 1 1 1 56 890131 131 PCT -4\SA\1\1>1486-M 3 102 2 2 74 3 1 1 57 890134 134 PCT -4\SA\1\1 >1549-M 7 92 2 2 76 3 1 1 1 58 890136 136 PCT -4\SA\1\1>1566-M 5 98 2 2 74 5 1 1 1 59 890137 137 PCT-4\SA\1\1>1576-M 5 98 2 2 74 3 1 1 1 60 890140 140 PCT -4\SA\1\1>1632-M 7 100 1 2 74 3 3 1 1 61 890145 145 PCT -4\SA\1\1>1689-M 5 89 2 2 71 3 1 1 1 62 890148 148 PCT -4\SA\ 1\1 >1702-M 5 91 2 2 72 3 1 1 63 890157 157 PCT -4\SA\1\1>1837-M 7 104 2 1 71 3 1 1 1 64 890164 164 PCT -4\SA\1\1>1878-M 5 98 74 5 1 1 1 65 890174 174 PCT -4\SA\1\1>1928-M 7 88 1 2 71 3 1 1 1 Vg = vigor; 81 1 = leaf blast ; 81 2 = leaf blast ; Fl = flowering; LSc = leaf scald; 8S = brown spot; NBI = neck blast; Gd = grain discoloration; Ht = plant height. Table 7. S4 line evaluation of the populations PCT-5\PHB\1\0; PCT-A\PHB\1 \0; and PCT -4\PHB\1\1 La Libertad experimental station, 1998A Nbr. Field Nbr. Origln Pedigree 1998A 19978 1 5840001 2 5840002 3 5840003 4 5840004 5 5840005 6 5840006 7 5840007 8 5840008 9 5840009 10 5840010 11 5840011 12 5840012 13 5840013 14 5840014 15 5840015 16 5840016 17 5840017 18 5840018 19 5840019 20 5840020 21 5840021 22 5840022 23 5840023 24 5840024 25 5840025 26 5840026 27 5840027 28 5840028 29 5840029 30 5840030 31 5840031 32 5840032 33 5840033 34 5840034 35 5840035 36 5840036 37 5840037 38 5840038 39 5840039 40 5840040 41 5840041 42 5840042 43 5840043 44 5840044 45 5840045 46 5840046 47 5840047 48 5840048 49 5840049 50 5840050 51 5840051 52 5840052 1 PCT-5\PHB\1\0>1165-M-1-M 2 PCT-5\PHB\1\0>1165-M-2-M 3 PCT-5\PHB\1\0>1165-M-3-M 4 PCT-5\PHB\1\0>1165-M-4-M 5 PCT-5\PHB\1 \0>1165-M-5-M 6 PCT-5\PHB\1\0>1165-M-6-M 7 PCT-A\PHB\1\0>161-M-1-M 8 PCT-A\PHB\1\0>161-M-2-M 9 PCT-A\PHB\1 \0>161-M-3-M 10 PCT-A\PHB\1 \0>161-M-4-M 11 PCT-A\PHB\1\0>161-M-5-M 12 PCT-A\PHB\1\0>161-M-6-M 13 PCT-A\PHB\1\0>1443-M-1-M 14 PCT-A\PHB\1\0>1443-M-2-M 15 PCT-A\PHB\1\0>1443-M-3-M 16 PCT-A\PHB\1\0>1443-M-4-M 17 PCT-A\PHB\1\0>1443-M-5-M 18 PCT-A\PHB\1 \0>1443-M-6-M 19 PCT -4\PHB\1\1>145-M-1-M 20 PCT-4\PHB\1\1>145-M-2-M 21 PCT -4\PHB\1\1>145-M-3-M 22 PCT -4\PHB\1 \1 >145-M-4-M 23 PCT -4\PHB\ 1\1 >145-M-5-M 24 PCT -4\PHB\1\1 >145-M-6-M 25 PCT -4\PHB\1\1>196-M-1-M 26 PCT-4\PHB\1 \1>196-M-2-M 27 PCT -4\PHB\ 1\1 >196-M-3-M 28 PCT -4\PHB\ 1\1 >196-M-4-M 29 PCT -4\PHB\1\1 >196-M-5-M 30 PCT -4\PHB\1\1>196-M-6-M 31 PCT -4\PHB\1\1>209-M-1-M 32 PCT -4\PHB\1\ 1 >209-M-2-M 33 PCT -4\PHB\1\1>209-M-3-M 34 PCT -4\PHB\1\1>209-M-4-M 35 PCT -4\PHB\ 1\1 >209-M-5-M 36 PCT-4\PHB\1\1>209-M-6-M 37 PCT-4\PHB\1\1>231-M-1-M 38 PCT -4\PHB\1\1>231-M-2-M 39 PCT -4\PHB\1\1 >231-M-3-M 40 PCT-4\PHB\1 \1>231-M-4-M 41 PCT-4\PHB\1\1>231-M-5-M 42 43 44 45 46 47 48 49 50 51 52 PCT -4\PHB\ 1\1 >231-M-6-M PCT -4\PHB\1\1>277-M-1-M PCT -4\PHB\1\1>277-M-2-M PCT -4\PHB\1\1 >277-M-3-M PCT -4\PHB\1\1>277-M-4-M PCT -4\PHB\1\1>277-M-5-M PCT -4\PHB\1\1>277-M-6-M PCT -4\PHB\1 \1>368-M-1-M PCT -4\PHB\1\1>368-M-2-M PCT -4\PHB\1\1>368-M-3-M PCT -4\PHB\1\1 >368-M-4-M Vg Bl Bl Fl LSc BS GD NBI 1 2 50% 5 5 5 78 3 1 3 7 4 5 79 1 1 1 7 3 2 67 1 1 3 1 3 4 3 79 1 1 1 1 5 2 2 82 1 1 1 1 7 3 5 80 1 1 1 1 7 2 3 67 1 1 3 1 7 5 5 71 1 1 1 1 5 5 5 75 1 1 1 3 3 4 75 7 3 3 82 7 4 4 77 5 3 3 69 5 3 3 79 5 2 2 68 5 3 3 77 7 1 1 76 5 3 80 5 1 1 75 7 4 4 79 5 5 5 70 5 5 5 79 7 3 3 70 5 3 3 79 7 5 4 80 7 4 4 81 3 5 5 82 1 1 1 1 3 1 1 3 1 1 3 1 3 3 1 1 5 1 1 3 1 1 3 1 3 3 1 3 1 1 3 3 1 1 1 1 1 1 3 1 1 3 1 1 3 1 1 3 1 5 4 4 71 3 1 5 4 3 82 7 4 3 83 7 4 4 84 7 4 3 79 5 5 4 80 5 3 3 77 7 3 2 71 7 2 1 75 5 2 2 77 7 2 2 75 5 5 4 66 5 5 4 81 5 3 3 87 3 3 2 71 5 3 2 77 5 3 2 82 5 3 3 82 5 71 5 1 78 5 2 68 5 3 2 66 5 2 1 75 5 2 2 71 5 2 2 74 1 1 1 3 1 1 1 1 1 1 1 1 3 3 3 3 3 1 3 1 3 1 3 1 3 3 1 5 1 1 5 3 3 3 1 3 1 1 1 1 1 1 1 3 1 1 3 1 1 1 1 1 1 1 3 1 3 1 1 1 1 1 1 5 1 1 1 3 1 1 1 1 3 1 1 1 1 3 1 1 1 5 3 1 1 1 1 1 1 1 1 3 53 5840053 54 5840054 55 5840055 56 5840056 57 5840057 58 5840058 59 5840059 60 5840060 61 5840061 62 5840062 63 5840063 64 5840064 65 5840065 66 5840066 67 5840067 68 5840068 69 5840069 70 5840070 71 5840071 72 5840072 73 5840073 74 5840074 75 5840075 76 5840076 77 5840077 78 5840078 79 5840079 80 5840080 81 5840081 82 5840082 83 5840083 84 5840084 85 5840085 86 5840086 87 5840087 88 5840088 89 5840089 90 5840090 91 5840091 92 5840092 93 5840093 94 5840094 95 5840095 96 5840096 97 5840097 98 5840098 99 5840099 100 5840100 101 5840101 102 5840102 103 5840103 104 5840104 105 5840105 106 5840106 107 5840107 108 5840108 109 5840109 110 5840110 53 PCT -4\PHB\ 1\1 >368-M-5-M 54 PCT-4\PHB\1 \1>368-M-6-M 55 PCT -4\PHB\1\1>453-M-1-M 56 PCT -4\PHB\ 1\1 >453-M-2-M 57 PCT -4\PHB\1\1>453-M-3-M 58 PCT -4\PHB\ 1\1 >453-M-4-M 59 PCT -4\PHB\ 1\1 >453-M-5-M 60 PCT -4\PHB\ 1\1 >453-M-6-M 61 PCT-4\PHB\1\1>485-M-1-M 62 PCT -4\PHB\1\1>485-M-2-M 63 PCT -4\PHB\1\1>485-M-3-M 64 PCT-4\PHB\1\1>485-M-4-M 65 PCT-4\PHB\1\1>485-M-5-M 66 PCT -4\PHB\ 1\1 >485-M-6-M 67 PCT -4\PHB\1\1>538-M-1-M 68 PCT -4\PHB\1\1>538-M-2-M 69 PCT -4\PHB\ 1\1 >538-M-3-M 70 PCT -4\PHB\1\1>538-M-4-M 71 PCT -4\PHB\1\ 1 >538•M-5-M 72 PCT -4\PHB\1\1>538-M-6-M 73 PCT-4\PHB\1\1>582-M-1-M 7 4 PCT -4\PHB\ 1\1 >582-M-2-M 75 PCT-4\PHB\1 \1>582-M-3-M 76 PCT -4\PHB\1\1>582-M-4-M 77 PCT -4\PHB\1\1>582-M-5-M 78 PCT -4\PHB\1\1>582-M-6-M 79 PCT-4\PHB\1\1>603-M-1-M 80 PCT -4\PHB\1\1 >603-M-2-M 81 PCT -4\PHB\1\1 >603-M-3-M 82 PCT -4\PHB\1\1 >603-M-4-M 83 PCT -4\PHB\1\1>603-M-5-M 84 PCT -4\PHB\1\1>603-M-6-M 85 PCT -4\PHB\1\1>749-M-1-M 86 PCT-4\PHB\1\1>749-M-2-M 87 PCT -4\PHB\ 1\1 >749-M-3-M 88 PCT-4\PHB\1\1>749-M-4-M 89 PCT-4\PHB\1\1>749-M-5-M 90 PCT -4\PHB\1\1>749-M-6-M 91 PCT-4\PHB\1\1>751-M-1-M 92 PCT-4\PHB\1\1>751-M-2-M 93 PCT -4\PHB\1\1>751-M-3-M 94 PCT-4\PHB\1\1>751-M-4-M 95 PCT -4\PHB\1\1>751 -M-5-M 96 PCT -4\PHB\1\1>751-M-6-M 97 PCT-4\PHB\1\1>752-M-1-M 98 PCT -4\PHB\1\1>752-M-2-M 99 PCT -4\PHB\ 1\1 >752-M-3-M 100 PCT-4\PHB\1\1>752-M-4-M 101 PCT -4\PHB\ 1\1 > 752-M-5-M 102 PCT-4\PHB\1\1>752-M-6-M 103 PCT -4\PHB\1\1>783-M-1-M 104 PCT-4\PHB\1\1>783-M-2-M 105 PCT -4\PHB\1\1 >783-M-3-M 106 PCT -4\PHB\1\1 >783-M-4-M 107 PCT -4\PHB\1\1>783-M-5-M 108 PCT -4\PHB\ 1\1 > 783-M-6-M 109 PCT -4\PHB\1\1>822-M-1-M 110 PCT -4\PHB\1\1 >822-M-2-M 5 3 2 71 5 3 2 71 7 2 1 74 7 1 1 71 7 2 4 78 7 2 3 71 7 2 2 84 7 4 4 79 7 3 2 77 7 2 2 80 5 4 4 82 5 4 4 77 5 4 3 77 5 3 3 79 5 3 3 80 7 2 2 71 7 5 5 77 5 2 3 75 3 4 3 75 3 3 4 75 5 4 3 80 5 2 75 5 3 3 74 5 2 2 81 7 2 1 76 5 4 4 82 5 2 2 74 7 2 1 77 7 3 3 74 5 2 1 84 7 2 1 74 5 3 3 76 5 2 2 77 7 2 2 73 5 2 2 75 5 3 2 71 7 3 3 83 5 3 3 70 5 3 2 85 5 2 2 75 3 1 67 5 2 79 3 2 1 79 5 2 2 75 5 2 1 68 5 2 67 5 2 1 65 3 1 2 76 5 1 1 78 5 2 1 77 5 4 3 77 7 2 74 5 3 2 85 5 2 77 7 2 1 68 7 2 70 7 2 70 7 2 75 1 1 3 3 1 1 1 1 3 1 1 1 3 1 1 1 1 1 1 1 3 1 1 1 3 1 1 3 5 1 3 3 1 1 1 1 1 1 1 1 3 1 3 1 1 3 1 3 1 3 1 1 1 1 3 1 1 3 1 1 3 1 1 3 1 5 1 1 1 1 3 1 1 1 1 1 3 1 3 3 3 3 1 1 1 1 3 1 3 3 1 3 1 3 3 3 3 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 3 1 1 1 1 1 1 1 1 3 1 3 1 3 5 1 3 1 1 1 3 1 1 1 1 3 1 1 1 1 1 1 1 3 1 1 1 1 1 3 3 3 1 1 3 1 1 1 1 1 3 1 3 3 1 1 1 111 5840111 111 PCT -4\PHB\ 1\1 >822-M-3-M 112 5840112 112 PCT-4\PHB\1\1>822-M-4-M 113 5840113 113 PCT -4\PHB\ 1\1 >822-M-5-M 114 PCT -4\PHB\ 1\1 >822-M-6-M 115 PCT -4\PHB\ 1\1 >846-M-1 -M 116 PCT -4\PHB\1\1>846-M-2-M 117 PCT -4\PHB\ 1\1 >846-M-3-M 118 PCT -4\PHB\ 1\1 >846-M-4-M 119 PCT -4\PHB\ 1\1 >846-M-5-M 120 PCT -4\PHB\1\1>846-M-6-M 121 PCT-4\PHB\1\1>856-M-1-M 122 PCT -4\PHB\ 1\1 >856-M-2-M 123 PCT -4\PHB\1\1>856-M-3-M 124 PCT-4\PHB\1\1>856-M-4-M 125 PCT -4\PHB\1\1 >856-M-5-M 126 PCT -4\PHB\1\1>856-M-6-M 127 PCT -4\PHB\1\1 >994-M-1-M 128 PCT-4\PHB\1\1>994-M-2-M 129 PCT -4\PHB\1\1>994-M-3-M 130 PCT -4\PHB\1\1 >994-M-4-M 131 PCT-4\PHB\1\1>994-M-5-M 132 PCT -4\PHB\1\1>994-M-6-M 133 PCT -4\PHB\1\1>1332-M-1-M 134 PCT -4\PHB\1\1>1332-M-2-M 135 PCT-4\PHB\1\1>1332-M-3-M 136 PCT-4\PHB\1\1>1332-M-4-M 137 PCT -4\PHB\1\1>1332-M-5-M 138 PCT -4\PHB\1\1>1332-M-6-M 139 PCT -4\PHB\1\1>1678-M-1-M 140 PCT -4\PHB\1\1>1678-M-2-M 141 PCT -4\PHB\1\1>1678-M-3-M 142 PCT -4\PHB\ 1\1 >1678-M-4-M 143 PCT -4\PHB\ 1\1 >1678-M-5-M 144 PCT -4\PHB\ 1\1 >1678-M-6-M 145 PCT -4\PHB\1\1>1723-M-1-M 146 PCT -4\PHB\1\1 >1723-M-2-M 5 1 1 72 1 1 1 1 5 3 3 71 1 5 22 841 5 1 1 74 5 4 4 74 5 3 2 82 5 3 1 79 5 2 2 70 5 2 2 72 5 2 3 74 5 3 2 76 7 2 2 70 5 2 2 81 5 3 2 74 7 2 2 82 5 3 2 77 5 2 2 85 7 1 1 ' 81 7 1 1 84 5 2 2 80 5 2 1 79 7 3 2 70 5 3 2 71 7 4 3 71 5 3 2 74 5 2 1 67 7 2 2 71 5 2 74 5 4 4 84 7 3 2 82 5 3 3 89 5 3 2 66 5 4 3 82 5 4 4 83 5 4 4 82 5 3 2 81 1 1 3 1 1 3 3 3 3 1 3 1 3 1 1 3 1 3 1 1 3 1 3 3 1 1 3 1 1 1 1 1 1 1 3 1 1 3 1 1 1 1 1 3 1 1 3 3 1 1 1 1 1 1 3 1 3 1 3 1 3 3 1 3 1 3 1 1 3 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 114 5840114 115 5840115 116 5840116 117 58401 17 118 5840118 119 5840119 120 5840120 121 5840121 122 5840122 123 5840123 124 5840124 125 5840125 126 58401 26 127 5840127 128 5840128 129 5840129 130 5840130 131 5840131 132 5840132 133 5840133 134 5840134 135 5840135 136 58401 36 137 5840137 138 5840138 139 5840139 140 5840140 141 5840141 142 5840142 143 5840143 144 5840144 145 5840145 146 5840146 147 5840147 148 5840148 149 5840149 150 5840150 147 PCT -4\PHB\1\1 >1723-M-3-M 5 3 2 75 3 1 1 148 PCT -4\PHB\1\1>1723-M-4-M 5 2 2 77 3 149 PCT -4\PHB\1\1>1723-M-5-M 5 2 3 75 3 150 PCT-4\PHB\1\1>1723-M-6-M 5 3 3 78 3 Vg = vigor: Bl 1 = leaf blast ; Bl 2 = leaf blast ; Fl = flowering; LSc = leaf scald; BS = brown spot; NBI = neck blast; Gd = grain discoloratlon. 1 1 1 Table 8. S41ines selected in the population PCT-4\PHB\1\1 . Nbr. La Libertad experimental station , 1998A Field Nbr. 1998A 1 S840021 2 5840023 3 5840028 4 5840036 5 5840037 6 5840042 7 5840045 8 5840046 9 5840047 10 5840048 11 5840049 12 5840050 13 5840054 14 5840055 15 5840056 16 5840062 17 5840068 18 5840079 19 5840083 20 5840086 21 5840093 22 5840095 23 5840097 24 5840099 25 5840100 26 5840103 27 5840106 28 5840107 29 5840109 30 5840114 31 5840118 32 S840142 33 5840145 34 5840146 35 5840147 Origin 19978 21 23 28 36 37 42 45 46 47 48 49 50 54 55 56 62 68 79 83 86 93 95 97 99 100 103 106 107 109 114 118 142 145 146 147 Pedigree PCT -4\PHB\1 \1 >145-M-3-M PCT -4\PHB\1\1>145-M-5-M PCT-4\PHB\1\1>196-M-4-M PCT -4\PHB\ 1\1 >209-M-6-M PCT -4\PHB\1\1>231-M-1-M PCT-4\PHB\1\1>231-M-6-M PCT -4\PHB\1\1>277-M-3-M PCT -4\PHB\ 1\1 >277 -M-4-M PCT -4\PHB\1\1>277-M-5-M PCT -4\PHB\ 1\1 >277 -M-6-M PCT -4\PHB\1\1>368-M-1-M PCT -4\PHB\ 1\1 >368-M-2-M PCT -4\PHB\ 1\1 >368-M-6-M PCT -4\PHB\ 1\1 >453-M-1-M PCT -4\PHB\ 1\1 >453-M-2-M PCT -4\PHB\1\1>485-M-2-M PCT -4\PHB\1\1>538-M-2-M PCT -4\PHB\1\1>603-M-1-M PCT -4\PHB\ 1\1 >603-M-5-M PCT -4\PHB\ 1\1 >749-M-2-M PCT -4\PHB\1\1>751-M-3-M PCT-4\PHB\1\1>751-M-5-M PCT -4\PHB\1\1>752-M-1-M PCT -4\PHB\1\1>752-M-3-M PCT -4\PHB\1\1>752-M-4-M PCT -4\PHB\ 1\1 > 783-M-1-M PCT -4\PHB\ 1\1 > 783-M-4-M PCT -4\PHB\1\1>783-M-5-M PCT -4\PHB\ 1\1 >822-M-1-M PCT -4\PHB\ 1\1 >822-M-6-M PCT -4\PHB\1\1>846-M-4-M PCT -4\PHB\1\1>1678-M-4-M PCT -4\PHB\1\1>1723-M-1-M PCT -4\PHB\1\1>1723-M-2-M PCT -4\PHB\1\1>1723-M-3-M Vg Bl Bl Fl LSc BS GD NBI 1 2 50% 5 5 5 70 3 3 1 1 7 3 3 70 1 1 1 1 5 4 4 71 7 2 1 75 5 2 2 77 3 3 2 71 5 3 3 82 5 1 1 71 5 1 1 78 5 2 1 68 5 3 2 66 5 2 1 75 5 3 2 71 7 2 1 74 7 71 7 2 2 80 7 2 2 71 5 2 2 74 7 2 1 74 7 2 2 73 3 1 1 67 3 2 1 79 5 2 1 68 5 2 1 65 3 1 2 76 5 4 3 77 5 2 1 77 7 2 1 68 7 2 1 70 5 1 1 74 5 2 2 70 5 3 2 66 5 4 4 82 5 3 2 81 5 3 2 75 3 1 1 1 3 1 1 1 3 1 1 1 3 1 1 1 1 1 3 1 1 3 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 1 3 1 1 1 1 3 1 1 1 1 1 1 1 3 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 3 1 1 3 1 1 3 1 1 3 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 3 1 1 1 1 1 1 1 Vg = vigor; 811 = leaf blast ; Bl 2 = leaf blast ; Fl = flowering; LSc = leaf scald; BS = brown spot; NBI = neck blast; Gd = grain discoloration. Table 9. S6 line evaluation from the populations PCT-5\0\0\0; PCT-A\0\0\0; and PCT -4\0\0\1 La libertad experimental station, 1998A Nbr. Field Nbr. Orlgln Pedigree 1998A. 1997B 1 S860001 1 PCT-5\0\0\0>1496-M-1-M-1-M 2 S860002 2 PCT-5\0\0\0>1496-M-1-M-2-M 3 S860003 3 PCT-5\0\0\0>1496-M-1-M-3-M 4 S860004 5 S860005 6 S860006 7 S860007 8 S860008 9 S860009 10 S860010 11 S860011 12 S860012 13 S860013 14 S860014 15 S860015 16 S860016 17 S860017 18 S860018 19 S860019 20 S860020 21 S860021 22 S860022 23 S860023 24 S860024 25 S860025 26 S860026 27 S860027 28 S860028 29 S860029 30 S860030 31 S860031 32 S860032 33 S860033 34 S860034 35 S860035 36 S860036 37 S860037 38 S860038 39 S860039 40 S860040 4 PCT-5\0\0\0>1496-M-1-M-4-M 5 PCT -5\0\0\0> 1496-M-1-M-5-M 6 PCT-5\0\0\0>1496-M-1-M-6-M 7 PCT-5\0\0\0>2130-M-2-M-1-M 8 PCT-5\0\0\0>2130-M-2-M-2-M 9 PCT-5\0\0\0>2130-M-2-M-3-M 1 O PCT -5\0\0\0>2130-M-2-M-4-M 11 PCT-5\0\0\0>2130-M-2-M-5-M 12 PCT -5\0\0\0>2130-M-2-M-6-M 13 PCT-5\0\0\0>2314-M-1-M-1-M 14 PCT -5\0\0\0>2314-M-1 -M-2-M 15 PCT-5\0\0\0>2314-M-1-M-3-M 16 PCT -5\0\0\0> 2314-M-1-M-4-M 17 PCT -5\0\0\0> 2314-M-1-M-5-M 18 PCT-5\0\0\0>2314-M-1-M-6-M 19 PCT-A.\0\0\0>175-M-1-M-1-M 20 PCT-A.\0\0\0>175-M-1-M-2-M 21 PCT-A.\0\0\0>175-M-1-M-3-M 22 PCT-A.\0\0\0>175-M-1-M-4-M 23 PCT-A.\0\0\0>175-M-1-M-5-M 24 PCT-A.\0\0\0>175-M-1-M-6-M 25 PCT-A.\0\0\0>175-M-3-M-1-M 26 PCT-A.\0\0\0>175-M-3-M-2-M 27 PCT-A\0\0\0>175-M-3-M-3-M 28 PCT -A\0\0\0>175-M-3-M-4-M 29 PCT -A\0\0\0>175-M-3-M-5-M 30 PCT-A.\0\0\0>175-M-3-M-6-M 31 PCT-A\0\0\0>175-M-4-M-1-M 32 PCT -A\0\0\0>175-M-4-M-2-M 33 PCT -A\0\0\0>175-M-4-M-3-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 34 PCT-A\0\0\0>175-M-4-M-4-M 35 PCT-A\0\0\0>175-M-4-M-5-M 36 PCT-A\0\0\0>175-M-4-M-6-M 37 PCT-A\0\0\0>175-M-6-M-1-M 38 PCT-A\0\0\0>175-M-6-M-2-M 39 PCT-A\0\0\0>175-M-6-M-3-M 40 PCT-A\0\0\0>1 75-M-6-M-4-M Vg Bl Bl 1 2 Fl LSc BS GD NBI Sal. 50% 3 3 3 84 3 3 1 1 5 2 2 78 3 1 . 1 1 5 2 2 77 31116 5 1 1 77 7 2 2 77 7 2 2 76 7 2 2 78 7 2 2 82 7 3 3 81 9 5 5 70 7 5 5 71 5 4 5 85 5 4 5 78 7 3 3 82 5 2 1 76 5 2 1 68 7 2 1 71 5 2 1 66 5 2 2 67 5 1 2 67 7 3 3 68 7 3 2 66 7 3 2 68 7 4 3 71 7 3 2 66 7 3 4 83 5 3 2 70 5 2 70 7 2 1 77 5 2 1 74 7 3 2 79 7 2 2 78 7 2 1 77 3 3 4 87 5 1 76 7 2 3 95 3 3 3 86 5 1 1 74 3 2 2 91 7 2 1 71 7 1 1 75 7 1 1 75 5 1 72 5 1 1 76 5 3 4 88 3 2 2 84 3 1 1 3 1 1 1 3 1 1 3 1 1 1 3 1 1 1 3 1 5 3 1 1 1 5 3 1 1 3 1 1 3 1 1 3 3 1 3 1 1 1 3 1 1 1 3 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 6 1 1 1 1 6 1 1 1 1 1 1 1 6 1 1 1 1 3 1 1 6 3 1 1 1 6 3 1 1 5 3 1 5 1 1 3 1 1 1 3 1 1 1 3 1 1 3 1 1 1 1 3 1 1 3 1 1 1 5 1 1 1 6 1 1 6 1 1 1 1 1 6 1 1 1 1 3 3 5 1 1 1 41 S860041 42 S860042 43 S860043 44 S860044 45 S860045 46 S860046 47 S860047 48 S860048 49 S860049 50 S860050 51 S860051 52 S860052 53 S860053 54 S860054 55 S860055 56 S860056 57 S860057 58 S860058 59 S860059 60 S860060 61 S860061 62 S860062 63 S860063 64 S860064 65 S860065 66 S860066 67 S860067 68 S860068 69 S860069 70 S860070 71 S860071 72 S860072 73 S860073 74 S860074 75 S860075 76 S860076 77 S860077 78 S860078 79 S860079 80 5860080 81 5860081 82 5860082 83 5860083 84 5860084 85 5860085 86 5860086 87 5860087 88 5860088 89 5860089 41 PCT-A\0\0\0>175-M-6-M-5-M 42 PCT-A\0\0\0>175-M-6-M-6-M 43 PCT-A\0\0\0>189-M-1-M-1-M 44 PCT-A\0\0\0>189-M-1-M-2-M 45 PCT-A\0\0\0>189-M-1-M-3-M 46 PCT-A\0\0\0>189-M-1-M-4-M 4 7 PCT -A \0\0\0> 189-M-1-M-5-M 48 PCT -A\0\0\0>189-M-1-M-6-M 49 PCT-A\0\0\0>189-M-2-M-1-M 50 PCT-A\0\0\0>189-M-2-M-2-M 51 PCT-A\0\0\0>189-M-2-M-3-M 52 PCT-A\0\0\0>189-M-2-M-4-M 53 PCT-A\0\0\0>189-M-2-M-5-M 54 PCT-A\0\0\0>189-M-2-M-6-M 55 PCT-A\0\0\0>189-M-3-M-1-M 56 PCT-A\0\0\0>189-M-3-M-2-M 57 PCT-A\0\0\0>189-M-3-M-3-M 58 PCT-A\0\0\0>189-M-3-M-4-M 59 PCT-A\0\0\0>189-M-3-M-5-M 60 PCT-A\0\0\0>189-M-3-M-6-M 61 PCT-A\0\0\0>189-M-4-M-1-M 62 PCT-A\0\0\0>189-M-4-M-2-M 63 PCT-A\0\0\0>189-M-4-M-3-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 64 PCT-A\0\0\0>189-M-4-M-4-M 65 PCT-A\0\0\0>189-M-4-M-5-M 66 PCT-A\0\0\0>189-M-4-M-6-M 67 PCT-A\0\0\0>278-M-1-M-1-M 68 PCT-A\0\0\0>278-M-1-M-2-M 69 PCT-A\0\0\0>278-M-1-M-3-M 70 PCT-A\0\0\0>278-M-1-M-4-M 71 PCT-A\0\0\0>278-M-1-M-5-M 72 PCT-A\0\0\0>278-M-1-M-6-M 73 PCT-A\0\0\0>394-M-1 -M-1-M 74 PCT-A\0\0\0>394-M-1-M-2-M 75 PCT-A\0\0\0>394-M-1-M-3-M 76 PCT-A\0\0\0>394-M-1-M-4-M 77 PCT-A\0\0\0>394-M-1-M-5-M 78 PCT-A\0\0\0>394-M-1-M-6-M 79 PCT-A\0\0\0>394-M-2-M-1-M 80 PCT-A\0\0\0>394-M-2-M-2-M 81 PCT-A\0\0\0>394-M-2-M-3-M 82 PCT -A\0\0\0>394-M-2-M-4-M 83 PCT-A\0\0\0>394-M-2-M-5-M 84 PCT-A\0\0\0>394-M-2-M-6-M 85 PCT-A\0\0\0>503-M-1-M-1-M 86 PCT-A\0\0\0>503-M-1-M-2-M 87 PCT-A\0\0\0>503-M-1-M-3-M 88 PCT-A\0\0\0>503-M-1-M-4-M 89 PCT-A\0\0\0>503-M-1-M-5-M 3 2 2 83 3 3 3 84 5 2 2 84 5 3 3 83 5 3 2 87 5 2 1 78 7 2 1 76 7 3 2 71 7 2 78 7 2 1 85 7 3 2 63 7 3 2 56 7 3 2 65 3 4 4 70 5 3 2 64 5 3 2 85 5 2 2 79 5 3 2 65 5 3 2 65 5 3 2 82 5 2 2 81 5 2 2 82 3 3 2 79 3 3 3 85 3 1 1 70 3 3 3 90 3 4 3 79 3 4 4 79 3 4 4 79 3 3 3 79 3 3 3 79 5 2 3 84 5 2 3 84 5 3 3 84 5 3 3 81 5 3 3 82 5 2 2 87 5 2 3 82 5 2 2 75 5 2 3 82 3 2 3 84 5 2 3 79 5 2 2 84 5 3 2 79 5 2 2 82 5 3 2 81 5 3 2 79 5 2 2 81 5 2 82 5 2 1 86 5 3 2 83 5 3 2 90 5 3 1 1 3 3 1 1 3 1 1 3 3 1 3 1 3 1 1 1 1 1 1 3 1 1 3 1 1 3 1 1 1 6 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 3 1 1 1 3 1 1 1 3 1 1 1 3 1 1 3 1 1 3 1 1 1 5 1 1 1 6 5 1 1 1 3 1 . 1 5 1 5 1 1 1 6 5 3 1 1 6 5 3 1 6 5 1 6 5 1 1 6 1 1 1 3 1 1 1 1 1 3 1 1 1 3 1 1 6 3 1 1 1 3 1 1 3 1 1 1 6 3 1 6 3 1 3 1 1 6 3 1 6 3 1 1 6 3 1 1 1 1 1 1 1 1 1 1 1 6 1 1 1 1 1 1 1 1 1 1 90 S860090 90 PCT-A\0\0\0>503-M-1 -M-6-M 91 S860091 92 S860092 93 S860093 94 S860094 95 S860095 96 S860096 97 S860097 98 S860098 99 S860099 100 S860100 101 S860101 102 S860102 103 S860103 104 S860104 105 S860105 106 S860106 107 S860107 108 S860108 109 S860109 110 S860110 111 S860111 112 S860112 113 S860113 114 5860114 115 S860115 116 S860116 117 S860117 118 S860118 119 S860119 120 S860120 121 S860121 122 S86012.2 123 S860123 124 S860124 125 S860125 126 S860126 127 S860127 128 S8601.28 129 S860129 130 S860130 131 S860131 132 S860132 Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 91 PCT-A\0\0\0>1169-M-1-M-1-M 92 PCT -A \0\0\0>1169-M-1-M-2-M 93 PCT-A\0\0\0>1169-M-1-M-3-M 94 PCT-A\0\0\0>1169-M-1-M-4-M 95 PCT-A\0\0\0>1169-M-1-M-5-M 96 PCT-A\0\0\0>1169-M-1-M-6-M 97 PCT-A\0\0\0>1169-M-2-M-1-M 98 PCT-A\0\0\0>1169-M-2-M-2-M 99 PCT-A\0\0\0>1169-M-2-M-3-M 100 PCT-A\0\0\0>1169-M-2-M-4-M 101 PCT-A\0\0\0>1169-M-2-M-5-M 102 PCT -A\0\0\0>1169-M-?-M-6-M 103 PCT-A\0\0\0>1321-M-2-M-1-M 104 PCT-A\0\0\0>1321-M-2-M-2-M 105 PCT-A\0\0\0>1321-M-2-M-3-M 106 PCT -A\0\0\0>1321-M-2-M-4-M 1 07 PCT -A\0\0\0>1321-M-2-M-5-M 108 PCT-A\0\0\0>1321-M-2-M-6-M 109 PCT-A\0\0\0>1452-M-1-M-1-M 110 PCT-A\0\0\0>1452-M-1-M-2-M 111 PCT -A\0\0\0>1452-M-1-M-3-M 112 PCT-A\0\0\0>1452-M-1-M-4-M 113 PCT-A\0\0\0>1452-M-1-M-5-M 114 PCT-A\0\0\0>1452-M-1-M-6-M 115 PCT -A \0\0\0> 1485-M-1-M-1-M 116 PCT -A\0\0\0>1485-M-1-M-2-M 117 PCT-A\0\0\0>1485-M-1-M-3-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O Oryzica Sabana 6 linea 30 Oryzica Sabana 1 O 118 PCT -A \0\0\0> 1485-M-1-M-4-M 119 PCT-A\0\0\0>1485-M-1-M-5-M 120 PCT -A \0\0\0> 1485-M-1-M-6-M 121 PCT-A\0\0\0>1488-M-4-M-1-M 122 PCT-A\0\0\0>1488-M-4-M-2-M 123 PCT-A\0\0\0>1488-M-4-M-3-M 124 PCT-A\0\0\0>1488-M-4-M-4-M 125 PCT -A \0\0\0> 1488-M-4-M-5-M 126 PCT-A\0\0\0>1488-M-4-M-6-M 127 PCT -A\0\0\0>1488-M-5-M-1-M 128 PCT-A\0\0\0>1488-M-5-M-2-M 129 PCT-A\0\0\0>1488-M-5-M-3-M 130 PCT -A \0\0\0> 1488-M-5-M-4-M 131 PCT-A\0\0\0>1488-M-5-M-5-M 132 PCT-A\0\0\0>1488-M-5-M-6-M 5 3 2 79 1 1 3 4 3 5 1 3 2 2 5 2 1 5 2 1 3 2 1 3 2 1 5 1 1 5 1 1 5 1 2 5 2 1 5 3 3 5 3 2 3 2 1 5 2 3 5 2 2 5 1 5 1 1 5 1 1 5 1 5 2 1 5 2 1 5 1 1 5 1 1 5 2 2 5 1 1 5 1 1 5 1 1 5 1 1 7 3 2 3 3 3 7 7 2 2 3 3 3 5 1 1 5 2 2 3 1 1 5 1 1 5 5 2 1 5 1 1 3 2 2 3 3 3 7 2 5 1 1 5 2 2 5 2 2 7 5 7 1 1 7 86 71 90 88 87 71 71 77 65 66 70 82 83 79 83 79 84 70 69 71 68 68 68 70 70 75 70 70 77 66 85 71 93 88 74 91 70 78 75 75 75 83 84 87 86 84 91 74 70 75 70 3 1 1 3 1 1 1 3 1 1 1 3 1 1 3 3 1 1 3 1 1 3 1 1 1 6 3 1 1 3 1 1 3 1 1 1 1 1 1 1 6 3 1 1 1 3 1 1 3 1 1 1 3 1 1 1 3 1 1 1 3 3 3 1 3 1 1 1 3 1 1 1 6 3 1 1 3 1 1 3 1 1 1 1 1 1 6 1 1 1 1 1 1 1 1 1 1 1 3 1 1 3 1 1 3 1 1 3 1 1 1 3 1 1 3 1 1 1 1 1 1 3 1 3 1 3 1 1 1 3 1 3 1 3 6 1 1 1 6 1 1 1 1 1 1 1 6 1 1 1 1 1 3 1 3 1 6 3 1 6 3 1 3 1 1 6 3 1 1 1 1 1 1 1 1 1 1 1 6 133 S860133 134 S860134 .135 S860135 136 S860136 137 S860137 138 S860138 139 S860139 140 S860140 141 S860141 142 S860142 143 S860143 144 S860144 145 5860145 146 S860146 147 S860147 148 S860148 149 S860149 150 S860150 151 S860151 152 S860152 153 S860153 154 S860154 155 S860155 156 S860156 157 S860157 158 S860158 159 S860159 160 S860160 161 S860161 162 S860162 163 5860163 164 S860164 165 S860165 166 S860166 167 S860167 168 S860168 169 S860169 170 S860170 171 S860171 172 S8601 72 173 S860173 174 S860174 175 S860175 176 S860176 177 S860177 178 S860178 133 PCT-A\0\0\0>1674-M-1-M-1-M 134 PCT-A\0\0\0>1674-M-1-M-2-M 135 PCT -A\0\0\0> 167 4-M-1-M-3-M 136 PCT -A\0\0\0> 167 4-M-1-M-4-M 137 PCT-A\0\0\0>1674-M-1-M-5-M 138 PCT -A\0\0\0>167 4-M-1-M-6-M 139 PCT-A\0\0\0>1674-M-2-M-1-M 140 PCT-A\0\0\0>1674-M-2-M-2-M 141 PCT-A\0\0\0>1674-M-2-M-3-M 142 PCT-A\0\0\0>1674-M-2-M-4-M 143 PCT-A\0\0\0>1674-M-2-M-5-M 144 PCT-A\0\0\0>1674-M-2-M-6-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 145 PCT-A\0\0\0>1674-M~M-1-M 146 PCT-A\0\0\0>1674-M-6-M-2-M 147 PCT-A\0\0\0>1674-M-6-M-3-M 148 PCT-A\0\0\0>1674-M-6-M-4-M 149 PCT-A\0\0\0>1674-M-6-M-5-M 150 PCT -A\0\0\0>1674-M-6-M-6-M 151 PCT-A\0\0\0>1788-M-2-M-1-M 152 PCT -A\0\0\0>1788-M-2-M-2-M 153 PCT-A\0\0\0>1788-M-2-M-3-M 154 PCT-A\0\0\0>1788-M-2-M-4-M 155 PCT-A\0\0\0>1788-M-2-M-5-M 156 PCT -A\0\0\0>1788-M-2-M-6-M 157 PCT-A\0\0\0>1788-M-3-M-1-M 158 PCT-A\0\0\0>1788-M-3-M-2-M 159 PCT-A\0\0\0>1788-M-3-M-3-M 160 PCT-A\0\0\0>1788-M-3-M-4-M 161 PCT-A\0\0\0>1788-M-3-M-5-M 162 PCT-A\0\0\0>1788-M-3-M-6-M 163 PCT-A\0\0\0>1788-M-5-M-1-M 164 PCT-A\0\0\0>1788-M-5-M-2-M 165 PCT-A\0\0\0>1788-M-5-M-3-M 166 PCT-A\0\0\0>1788-M-5-M-4-M 167 PCT-A\0\0\0>1788-M-5-M-5-M 168 PCT-A\0\0\0>1788-M-5-M-6-M 169 PCT -A\0\0\0>1832-M-1-M-1-M 170 PCT -A\0\0\0> 1832-M-1-M-2-M 171 PCT-A\0\0\0>1832-M-1-M-3-M Oryzica Sabana 6 Unea 30 Oryzica Sabana 1 O 172 PCT -A\0\0\0>1832-M-1-M-4-M 173 PCT -A\0\0\0> 1832-M-1-M-5-M 174 PCT-A\0\0\0>1832-M-1-M-6-M 175 PCT-A\0\0\0>1955-M-2-M-1-M 176 PCT -A\0\0\0>1955-M-2-M-2-M 177 PCT-A\0\0\0>1955-M-2-M-3-M 178 PCT-A\0\0\0>1955-M-2-M-4-M 7 1 1 7 1 5 2 3 5 3 4 5 3 4 5 3 3 5 3 3 7 4 4 3 3 3 5 2 1 5 2 2 3 2 2 3 4 4 5 2 2 5 3 3 3 2 3 5 2 1 5 3 3 5 5 5 7 5 5 5 3 2 5 3 4 9 4 4 7 3 2 5 2 1 7 2 1 7 2 1 5 2 1 7 2 1 7 2 1 5 3 2 5 2 1 5 3 1 5 2 1 5 2 1 5 4 4 3 4 4 5 4 4 3 4 4 3 5 5 5 4 4 5 3 3 3 4 3 3 1 1 5 3 3 5 3 2 5 2 2 5 2 1 5 2 2 3 1 1 3 1 2 3 3 2 67 76 83 86 84 82 82 88 78 71 82 75 86 75 93 84 88 70 70 79 71 73 83 68 65 78 70 65 74 71 70 70 71 70 71 79 79 80 80 80 81 84 86 70 90 85 85 85 85 86 81 81 1 1 1 1 1 1 3 1 1 1 3 1 1 1 3 3 1 .. 1 3 1 1 3 1 1 1 1 1 1 3 1 1 1 3 1 1 6 1 1 1 1 1 1 3 3 3 1 1 3 1 1 1 3 1 1 3 1 1 3 3 1 1 1 6 3 1 1 1 1 1 1 3 1 1 1 6 1 1 1 1 1 1 1 1 6 3 1 1 3 1 1 1 3 1 1 1 3 1 1 1 3 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 3 1 1 1 3 1 1 1 3 3 1 1 3 1 1 3 1 1 1 3 1 1 1 3 1 1 1 3 1 1 3 1 3 1 3 1 3 1 1 1 3 1 1 1 3 1 1 5 1 1 1 5 1 1 179 S860179 180 S860180 181 S860181 182 S860182 183 S860183 184 S860184 185 S860185 186 S860186 187 S860187 188 S860188 189 S860189 190 S860190 191 S860191 192 S860192 193 S860193 194 S860194 195 S860195 196 S860196 197 S860197 198 S860198 199 S860199 200 S860200 201 S860201 202 S860202 203 S860203 204 S860204 205 S860205 206 S860206 207 S860207 208 S860208 209 S860209 210 S860210 211 S860211 212 5860212 213 S860213 214 S860214 215 S860215 216 8860216 217 8860217 218 S860218 219 8860219 220 8860220 221 8860221 222 S860222 223 S860223 224 S860224 179 PCT-A\0\0\0>1955-M-2-M-5-M 180 PCT-A\0\0\0>1955-M-2-M-6-M 181 PCT-A\0\0\0>1955-M-3-M-1-M 182 PCT-A\0\0\0>1955-M-3-M-2-M 183 PCT-A\0\0\0>1955-M-3-M-3-M 184 PCT -A\0\0\0>1955-M-3-M-4-M 185 PCT -A\0\0\0>1955-M-3-M-5-M 186 PCT-A\0\0\0>1955-M-3-M-6-M 187 PCT-A\0\0\0>1955-M-4-M-1-M 188 PCT-A\0\0\0>1955-M-4-M-2-M 189 PCT-A\0\0\0>1955-M-4-M-3-M 190 PCT -A\0\0\0>1955-M-4-M-4-M 191 PCT-A\0\0\0>1955-M-4-M-5-M 192 PCT -A\0\0\0>1955-M-4-M-6-M 193 PCT -A \0\0\0> 1955-M-5-M-1-M 194 PCT-A\0\0\0>1955-M-5-M-2-M 195 PCT-A\0\0\0>1955-M-5-M-3-M 196 PCT-A\0\0\0>1955-M-5-M-4-M 197 PCT-A\0\0\0>1955-M-5-M-5-M 198 PCT-A\0\0\0>1955-M-5-M-6-M 199 PCT-A\0\0\0>2083-M-1-M-1-M 200 PCT-A\0\0\0>2083-M-1-M-2-M 201 PCT -A \0\0\0> 2083-M-1-M-3-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 202 PCT-A\0\0\0>2083-M-1-M-4-M 203 PCT -A\0\0\0>2083-M-1-M-5-M 204 PCT-A\0\0\0>2083-M-1-M-6-M 205 PCT-A\0\0\0>2083-M-2-M-1-M 206 PCT-A\0\0\0>2083-M-2-M-2-M 207 PCT-A\0\0\0>2083-M-2-M-3-M 208 PCT-A\0\0\0>2083-M-2-M-4-M 209 PCT-A\0\0\0>2083-M-2-M-5-M 210 PCT-A\0\0\0>2083-M-2-M-6-M 211 PCT -A\0\0\0>2137 -M-2-M-1-M 212 PCT-A\0\0\0>2137-M-2-M-2-M 213 PCT-A\0\0\0>2137-M-2-M-3-M 214 PCT-A\0\0\0>2137-M-2-M-4-M 215 PCT-A\0\0\0>2137-M-2-M-5-M 216 PCT-A\0\0\0>2137-M-2-M-6-M 217 PCT-A\0\0\0>2149-M-1 -M-1-M 218 PCT-A\0\0\0>2149-M-1-M-2-M 219 PCT-A\0\0\0>2149-M-1-M-3-M 220 PCT-A\0\0\0>21 49-M-1-M-4-M 221 PCT-A\0\0\0>2149-M-1-M-5-M 222 PCT -A \0\0\0> 2149-M-1-M-6-M 223 PCT -A\0\0\0>2149-M-3-M-1-M 224 PCT-A\0\0\0>2149-M-3-M-2-M 3 3 2 3 3 2 3 2 2 3 2 2 3 2 2 3 2 1 5 2 1 5 2 1 5 2 1 5 2 1 3 2 1 3 2 1 5 2 5 2 1 3 2 1 3 2 1 5 1 1 3 1 5 1 1 5 1 1 5 1 1 7 1 1 7 1 1 3 3 3 5 1 1 5 3 3 3 3 3 5 1 1 5 2 3 5 1 1 7 1 1 5 1 1 5 1 1 5 1 1 3 1 1 5 1 1 5 5 1 1 5 2 1 5 1 1 5 1 1 5 1 1 5 1 5 2 1 5 1 1 5 1 1 3 2 2 3 2 3 1 3 1 1 3 2 1 5 2 2 82 5 1 1 83 5 3 1 82 5 3 1 83 5 1 1 83 3 1 1 78 5 1 1 80 3 1 1 83 3 1 1 80 3 1 1 82 3 1 1 81 3 1 1 74 5 1 1 80 5 1 1 75 5 1 1 82 5 1 1 82 5 1 1 76 3 1 1 72 3 1 1 68 3 1 1 68 3 1 1 67 3 1 1 68 3 1 1 65 3 1 1 86 3 1 3 71 3 1 1 90 3 1 3 84 3 1 3 73 3 1 1 89 3 1 1 66 3 1 1 68 3 1 1 67 3 1 1 64 3 1 1 63 3 1 1 63 3 1 1 67 3 1 1 67 3 1 1 73 3 1 . 1 64 3 1 1 73 3 1 1 75 3 1 1 68 3 1 1 70 3 1 66 33 1 71 3 1 74 3 71 5 1 71 5 74 5 1 71 5 1 74 5 1 1 81 5 1 1 1 1 1 1 6 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 6 1 6 1 6 1 6 1 6 1 6 1 1 1 1 1 1 1 1 1 1 1 1 1 f 1 1 f f ¿ 1 1 1 1 1 ! 1 225 5860225 226 5860226 227 5860227 228 5860228 229 5860229 230 5860230 231 5860231 232 5860232 233 S860233 234 5860234 235 S860235 236 5860236 237 S860237 238 S860238 239 5860239 240 5860240 241 S860241 242 5860242 243 S860243 244 S860244 245 5860245 246 S860246 247 S860247 248 S860248 249 S860249 250 S860250 251 S860251 252 S860252 253 S860253 254 S860254 255 S860255 256 S860256 257 S860257 258 S860258 259 S860259 260 S860260 261 5860261 262 5860262 263 5860263 264 S860264 265 S860265 266 S860266 267 5860267 268 S860268 269 S860269 270 S860270 225 PCT-A\0\0\0>2149-M-3-M-3-M 226 PCT-A\0\0\0>2149-M-3-M-4-M 227 PCT-A\0\0\0>2149-M-3-M-5-M 228 PCT-A\0\0\0>2149-M-3-M-6-M 229 PCT -4\0\0\1>1311-M-1-M-1-M 230 PCT -4\0\0\1>1311-M-1-M-2-M 231 PCT -4\0\0\1>1311-M-1-M-3-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 232 PCT -4\0\0\1>1311-M-1-M-4-M 233 PCT -4\0\0\0> 1311-M-1-M-5-M 234 PCT -4\0\01>1311-M-1-M-6-M 235 PCT -4\0\0\1>1311-M-2-M-1-M 236 PCT -4\0\0\1>1311-M-2-M-2-M 237 PCT -4\0\0\1>1311-M-2-M-3-M 238 PCT -4\0\0\1 >1311-M-2-M-4-M 239 PCT -4\0\0\1 >1311-M-2-M-5-M 240 PCT -4\0\0\1>1311-M-2-M-6-M 241 PCT -4\0\0\1 >90-M-2-M-1-M 242 PCT -4\0\0\1 >90-M-2-M-2-M 243 PCT -4\0\0\1>90-M-2-M-3-M 244 PCT -4\0\0\1 >90-M-2-M-4-M 245 PCT -4\0\0\1>90-M-2-M-5-M 246 PCT -4\0\0\1>90-M-2-M-6-M 247 PCT-4\0\0\1>106-M-3-M-1-M 248 PCT-4\0\0\1>106-M-3-M-2-M 249 PCT -4\0\0\1 >106-M-3-M-3-M 250 PCT -4\0\0\1 >1 06-M-3-M-4-M 251 PCT -4\0\0\1 >1 06-M-3-M-5-M 252 PCT -4\0\0\\1 >1 06-M-3-M-6-M 253 PCT -4\0\0\0\1 >2435-M-2-M-1-M 254 PCT -4\0\0\0\1 >2435-M-2-M-2-M 255 PCT -4\0\0\0\1 >2435-M-2-M-3-M 256 PCT -4\0\0\0\1 >2435-M-2-M-4-M 257 PCT -4\0\0\0\1>2435-M-2-M-5-M 258 PCT -4\0\0\0\1 >2435-M-2-M-6-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 259 PCT -4\0\0\0\1>2485-M-1-M-1-M 260 PCT-4\0\0\0\1>2485-M-1-M-2-M 261 PCT -4\0\0\0\1 >2485-M-1-M-3-M 262 PCT -4\0\0\0\1 >2485-M-1-M-4-M 263 PCT -4\0\0\0\1 >2485-M-1-M-5-M 264 PCT -4\0\0\0\1>2485-M-1-M-6-M 265 PCT -4\0\0\0\1>2485-M-2-M-1-M 266 PCT -4\0\0\0\1 >2485-M-2-M-2-M 267 PCT -4\0\0\0\1 >2485-M-2-M-3-M 268 PCT -4\0\0\0\1 >2485-M-2-M-4-M 269 PCT -4\0\0\0\1 >2485-M-2-M-5-M 270 PCT -4\0\0\0\1>2485-M-2-M-6-M 5 1 5 1 1 5 1 1 5 1 1 5 1 1 5 1 1 5 1 1 3 3 3 5 1 5 2 2 5 2 1 5 2 1 5 1 5 1 5 1 3 2 1 3 2 1 7 2 1 5 2 1 5 2 1 5 2 1 5 2 1 5 2 1 7 2 1 5 2 1 5 2 1 7 2 1 5 2 5 1 1 5 2 2 5 1 2 5 1 1 5 1 1 7 1 1 7 1 1 7 1 2 7 1 1 3 3 4 5 1 1 5 3 3 5 1 1 7 1 1 3 3 4 3 3 4 5 3 4 3 3 4 5 3 3 5 3 3 5 4 4 5 5 5 5 3 3 5 3 3 81 79 83 81 81 80 81 85 71 90 82 83 79 81 84 70 71 78 71 73 75 69 70 75 70 69 70 71 78 79 82 71 79 70 70 70 71 88 72 91 70 71 84 84 86 82 83 83 75 76 79 77 5 5 1 1 5 1 1 5 1 1 1 5 1 1 1 5 1 1 5 1 1 1 5 1 1 3 1 1 3 1 1 3 3 1 1 5 1 1 1 5 1 1 1 3 1 1 6 3 1 1 3 1 1 3 1 1 1 3 1 1 3 1 1 1 3 1 1 1 3 1 1 3 1 1 1 6 3 1 6 3 1 6 3 1 1 6 3 1 1 6 3 1 1 1 3 1 1 1 6 3 3 1 1 3 3 1 1 3 3 1 5 3 1 1 3 3 1 3 1 1 1 6 3 1 1 3 3 1 1 3 3 3 1 6 3 3 3 1 3 1 3 3 1 1 3 3 1 1 6 3 3 1 1 5 1 1 5 1 5 1 5 6 5 1 1 1 5 1 1 1 6 3 3 1 6 3 3 1 6 3 3 1 1 3 1 1 3 271 S860271 271 PCT -4\0\0\0\1 >2485-M-3-M-1-M 5 2 3 77 3 1 6 272 S860272 272 PCT -4\0\0\0\1 >2485-M-3-M-2-M 7 2 2 79 3 1 1 1 273 S860273 273 PCT -4\0\0\0\1 >2485-M-3-M-3-M 5 2 2 74 3 1 1 6 274 S860274 274 PCT -4\0\0\0\1 > 2485-M-3-M-4-M 5 1 2 75 3 1 6 275 S860275 275 PCT -4\0\0\0\1 >2485-M-3-M-5-M 7 80 1 1 276 S860276 276 PCT -4\0\0\0\1 >2485-M-3-M-6-M 5 1 1 75 1 1 1 1 277 S860277 277 PCT -4\0\0\0\1 > 2486-M-1-M-1-M 5 1 1 75 1 1 1 1 6 278 S860278 278 PCT -4\0\0\0\1 >2486-M-1-M-2-M 7 1 75 1 279 S860279 279 PCT -4\0\0\0\1>2486-M-1-M-3-M 5 1 81 1 1 280 S860280 280 PCT -4\0\0\0\1 >2486-M-1-M-4-M 5 79 3 1 1 1 281 S860281 281 PCT -4\0\0\0\1 >2486-M-1-M-5-M 7 83 3 1 1 282 S860282 282 PCT -4\0\0\0\1>2486-M-1-M-6-M 7 1 1 85 3 1 1 1 Vg = vigor; 81 1 = leaf blast ; 81 2 = leaf blast ; Fl = flowering; LSc = leaf scald; BS = brown spot; N81 = neck bias!; Gd = grain discoloratíon; Sel = plan! selectíon. Table 10. S61ines selected from the populations PCT-5\0\0\0; ; PCT-A\0\0\0; and PCT-4\0\0\1 La Libertad experimental station, 1998A Nbr. Field Nbr. Orlgin Pedigree 1998A 19978 1 5860003 3 PCT-5\0\0\0>1496-M-1-M-3-M 2 5860022 22 PCT-A\0\0\0>175-M-1-M-4-M 3 5860023 23 PCT-A\0\0\0>175-M-1-M-5-M 4 5860025 25 PCT-A\0\0\0>175-M-3-M-1-M 5 S860027 27 PCT-A\0\0\0>175-M-3-M-3-M 6 5860028 28 PCT-A\0\0\0>175-M-3-M-4-M 7 5860034 34 PCT-A\0\0\0>175-M-4-M-4-M 8 5860035 35 PCT-A\0\0\0>175-M-4-M-5-M 9 S860037 37 PCT-A\0\0\0>175-M-6-M-1-M 10 5860050 50 PCT-A\0\0\0>189-M-2-M-2-M 11 5860063 63 PCT-A\0\0\0>189-M-4-M-3-M 12 5860064 64 PCT-A\0\0\0>189-M-4-M-4-M 13 5860065 65 PCT-A\0\0\0>189-M-4-M-5-M 14 5860066 15 5860067 16 5860068 17 5860073 18 S860076 19 5860077 20 5860079 21 5860080 22 5860081 23 S860085 24 5860094 25 5860098 26 S860106 27 5860110 28 S860118 29 5860119 30 5860121 31 5860124 32 5860125 33 5860127 34 5860130 35 5860142 36 5860147 37 5860150 66 PCT-A\0\0\0>189-M-4-M-6-M 67 PCT-A\0\0\0>278-M-1-M-1-M 68 PCT-A\0\0\0>278-M-1-M-2-M 73 PCT-A\0\0\0>394-M-1-M-1-M 76 PCT-A\0\0\0>394-M-1-M-4-M 77 PCT-A\0\0\0>394-M-1-M-5-M 79 PCT -A\0\0\0>394-M-2-M-1-M 80 PCT-A\0\0\0>394-M-2-M-2-M 81 PCT -A\0\0\0>394-M-2-M-3-M 85 PCT -A\0\0\0>503-M-1-M-1-M 94 PCT-A\0\0\0>1169-M-1-M-4-M 98 PCT -A\0\0\0>1169-M-2-M-2-M 106 PCT -A\0\0\0>1321-M-2-M-4-M 11 O PCT -A\0\0\0>1452-M-1-M-2-M 118 PCT-A\0\0\0>1485-M-1-M-4-M 119 PCT-A\0\0\0>1485-M-1-M-5-M 121 PCT-A\0\0\0>1488-M-4-M-1-M 124 PCT-A\0\0\0>1488-M-4-M-4-M 125 PCT-A\0\0\0>1488-M-4-M-5-M 127 PCT-A\0\0\0>1488-M-5-M-1-M 130 PCT-A\0\0\0>1488-M-5-M-4-M 142 PCT-A\0\0\0>1674-M-2-M-4-M 147 PCT-A\0\0\0>1674-M-6-M-3-M 150 PCT-A\0\0\0>167 4-M-6-M-6-M 38 5860152 152 PCT-A\0\0\0>1788-M-2-M-2-M 39 5860184 184 PCT-A\0\0\0>1955-M-3-M-4-M 40 5860202 202 PCT-A\0\0\0>2083-M-1-M-4-M 41 5860203 203 PCT-A\0\0\0>2083-M-1-M-5-M 42 5860204 204 PCT-A\0\0\0>2083-M-1-M-6-M 43 5860205 205 PCT-A\0\0\0>2083-M-2-M-1-M 44 5860207 207 PCT-A\0\0\0>2083-M-2-M-3-M 45 5860208 208 PCT-A\0\0\0>2083-M-2-M-4-M 46 5860235 235 PCT -4\0\0\0>1311-M-2-M-1-M 47 5860243 243 PCT -4\0\0\0>90-M-2-M-3-M 48 5860244 244 PCT -4\0\0\0>90-M-2-M-4-M 49 S860245 245 PCT -4\0\0\0>90-M-2-M-5-M 50 5860246 246 PCT -4\0\0\0>90-M-2-M-6-M Vg Bl Bl Fl LSc BS GD NBI 1 2 50% 5 2 2 77% 3 1 1 1 7 3 2 66 1 1 1 7 3 2 7 3 2 5 3 2 68 1 1 1 1 66 1 1 1 1 5 2 7 2 7 5 1 1 7 2 1 1 1 1 3 3 2 3 4 3 3 4 4 3 4 4 3 3 3 3 3 3 5 3 3 5 2 2 5 2 3 5 2 3 5 2 2 5 3 2 5 2 2 3 2 5 2 1 5 1 5 1 1 3 1 1 5 1 1 5 2 1 3 3 3 7 2 1 5 2 2 5 1 1 5 2 1 5 3 3 5 3 2 70 70 71 75 72 85 79 79 79 79 79 79 82 75 82 79 84 79 81 71 70 69 68 70 78 75 84 87 84 70 71 70 71 9 4 4 83 3 2 1 78 5 1 1 66 7 1 1 68 5 1 1 67 5 1 1 64 3 1 1 63 5 1 1 67 3 1 3 1 1 1 1 1 1 3 1 5 1 5 1 5 3 5 3 5 1 5 1 3 1 3 1 3 1 3 1 3 1 3 1 1 1 3 1 1 3 1 1 1 1 1 1 1 1 1 3 1 3 1 3 1 1 3 1 3 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 5 1 1 3 1 3 1 1 1 3 1 1 1 3 1 1 3 1 3 1 1 5 1 1 81 3 1 1 1 5 2 1 69 3 1 1 1 5 2 70 3 1 7 2 75 3 5 2 70 3 1 51 5860247 247 PCT -4\0\0\0>1 06-M-3-M-1-M 5 2 69 3 1 1 52 5860249 249 PCT -4\0\0\0::>106-M-3-M-3-M 5 2 1 71 3 1 53 5860255 255 PCT -4\0\0\0\1 >2435-M-2-M-3-M 7 1 70 3 1 54 5860258 258 PCT -4\0\0\0\1 >2435-M-2-M-6-M 7 1 71 3 3 3 1 55 5860259 259 PCT -4\0\0\0\1>2485-M-1-M-1-M 5 1 70 3 3 1 56 5860264 264 PCT -4\0\0\0\1 >2485-M-1-M-6-M 3 3 4 82 5 57 5860266 266 PCT -4\0\0\0\1 >2485-M-2-M-2-M 5 3 3 83 5 1 58 5860267 267 PCT -4\0\0\0\1 > 2485-M-2-M-3-M 5 4 4 75 3 3 1 1 59 5860268 268 PCT -4\0\0\0\1 >2485-M-2-M-4-M 5 5 5 76 3 3 1 1 60 5860271 271 PCT -4\0\0\0\1>2485-M-3-M-1-M 5 2 3 77 3 1 1 61 5860273 273 PCT -4\0\0\0\1 >2485-M-3-M-3-M 5 2 2 74 3 1 1 1 62 5860274 274 PCT -4\0\0\0\1 >2485-M-3-M-4-M 5 1 2 75 3 1 1 1 63 5860277 277 PCT -4\0\0\0\1 >2486-M-1-M-1-M 5 1 1 75 1 1 1 1 Vg = vigor: Bl 1 = leaf blast ; Bl 2 = leaf blast ; Fl = flowering; LSc = leaf scald; BS = brown spot; NBI = neck blast; Gd = grain discoloration. Table 11 . 56 line evaluation from the population PCT -4\0\0\1 >52 La Libertad experimental station, 1998A Nbr. Field Nbr. Origin Pedigree 1998A 19978 1 5860283 283 PCT -4\0\0\1 >52-41-1-M-1-M 2 5860284 284 PCT -4\0\0\1 >52-41-1-M-2-M 3 5860285 285 PCT -4\0\0\1 >52-41-1-M-3-M 4 5860286 286 PCT -4\0\0\1 >52-41 -1-M-4-M 5 5860287 287 PCT -4\0\0\1 >52-41-1-M-5-M 6 5860288 288 PCT -4\0\0\1 >52-41 -1-M-6-M 7 5860289 289 PCT -4\0\0\1 >52-41-2-M-1-M 8 5860290 290 PCT -4\0\0\1>52-41-2-M-2-M 9 5860291 291 PCT -4\0\0\1 >52-41-2-M-3-M 10 5860292 11 5860293 12 5860294 13 5860295 14 5860296 15 5860297 16 5860298 17 5860299 18 5860300 19 5860301 20 5860302 21 5860303 22 5860304 23 5860305 24 5860306 25 5860307 26 5860308 27 5860309 28 5860310 29 5860311 30 5860312 31 5860313 32 5860314 33 5860315 34 5860316 35 5860317 36 5860318 37 5860319 38 5860320 39 5860321 40 S860322 41 5860323 42 5860324 292 PCT -4\0\0\1 >52-41-2-M-4-M 293 PCT -4\0\0\1 >52-41-2-M-5-M 294 PCT -4\0\0\1 >52-41-2-M-6-M 295 PCT -4\0\0\1>52-1532-1-M-1 -M 296 PCT -4\0\0\1 >52-1532-1-M-2-M 297 PCT -4\0\0\1 >S2-1532-1-M-3-M 298 PCT -4\0\0\1 >S2-1532-1-M-4-M 299 PCT -4\0\0\1 >S2-1532-1-M-5-M 300 PCT -4\0\0\1 >S2-1532-1-M-6-M 301 PCT -4\0\0\1 >S2-1803-3-M-1-M 302 PCT -4\0\0\1 >S2-1803-3-M-2-M 303 PCT -4\0\0\1 >S2-1803-3-M-3-M 304 PCT -4\0\0\1 >S2-1803-3-M-4-M 305 PCT -4\0\0\1>S2-1803-3-M-5-M 306 PCT -4\0\0\1>S2-1803-3-M-6-M 307 PCT -4\0\0\1 >S2-2324-2-M-1-M 308 PCT -4\0\0\1>S2-2324-2-M-2-M 309 PCT -4\0\0\1 >S2-2324-2-M-3-M 31 O PCT -4\0\0\1 >52-2324-2-M-4-M 311 PCT -4\0\0\1>S2-2324-2-M-5-M 312 PCT -4\0\0\1 >S2-2324-2-M-6-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 313 PCT -4\0\0\1>S2-2324-3-M-1 -M 314 PCT -4\0\0\1 >S2-2324-3-M-2-M 315 PCT -4\0\0\1 >S2-2324-3-M-3-M 316 PCT -4\0\0\1 >S2-2324-3-M-4-M 317 PCT -4\0\0\1 >S2-2324-3-M-5-M 318 PCT -4\0\0\1>S2-2324-3-M-6-M 319 PCT -4\0\0\1>52-2324-4-M-1-M 320 PCT -4\0\0\1 >S2-2324-4-M-2-M 321 PCT -4\0\0\1 >S2-2324-4-M-3-M 322 PCT -4\0\0\1 >S2-2324-4-M-4-M 323 PCT -4\0\0\1>S2-2324-4-M-5-M 324 PCT -4\0\0\1 >52-2324-4-M-6-M Vg Bl Bl Fl LSc BS GD NBI 1 2 50-J. 5 1 1 85 3 1 1 1 5 1 1 5 2 1 5 2 1 85 3 1 1 1 76 5 1 1 1 76 3 1 1 1 5 1 1 5 1 1 5 2 2 81 3 1 1 1 76 3 1 1 1 75 3 1 1 1 5 1 1 5 1 1 5 1 5 1 5 1 1 5 1 5 2 1 5 2 1 5 2 1 5 1 1 5 1 5 1 1 5 2 1 5 1 5 5 5 5 1 5 1 1 1 1 5 1 1 3 1 7 1 1 5 2 1 83 81 74 79 78 76 79 77 80 82 78 78 82 78 77 78 76 77 80 77 74 80 75 5 4 4 87 5 1 1 74 5 3 3 93 3 1 1 70 5 1 1 79 3 3 2 71 5 2 1 70 5 2 1 79 3 2 1 73 3 1 1 71 3 1 75 5 2 71 5 2 1 71 5 1 1 78 5 1 1 75 3 3 3 3 3 3 3 3 3 3 5 5 5 5 3 3 3 3 3 3 3 3 3 5 3 5 3 3 3 3 3 3 3 3 3 3 1 1 1 1 1 1 1 1 1 1 3 1 3 3 1 1 1 1 1 1 1 3 3 3 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 43 S860325 44 S860326 45 S860327 46 S860328 47 S860329 48 S860330 49 S860331 50 S860332 51 S860333 52 S860334 53 S860335 54 S860336 55 S860337 56 S860338 57 S860339 58 S860340 59 S860341 60 S860342 61 S860343 62 S860344 63 S860345 64 S860346 65 S860347 66 S860348 67 S860349 68 S860350 69 S860351 70 S860352 71 S860353 72 S860354 73 S860355 74 S860356 75 S860357 76 S860358 77 S860359 78 S860360 79 S860361 80 S860362 81 S860363 82 S860364 83 S860365 84 S860366 85 S860367 86 S860368 87 S860369 325 PCT -4\0\0\1 >S2-2324-5-M-1-M 326 PCT -4\0\0\1 >S2-2324-5-M-2-M 327 PCT -4\0\0\1 >S2-2324-5-M-3-M 328 PCT -4\0\0\1 >S2-2324-5-M-4-M 329 PCT -4\0\0\1 >S2-2324-5-M-5-M 330 PCT -4\0\0\1 >S2-2324-5-M-6-M 331 PCT -4\0\0\1 >S2-2358-2-M-1-M 332 PCT -4\0\0\1>S2-2358-2-M-2-M 333 PCT -4\0\0\1>S2-2358-2-M-3-M 334 PCT -4\0\0\1 >S2-2358-2-M-4-M 335 PCT -4\0\0\1>S2-2358-2-M-5-M 336 PCT-4\0\0\1>S2-2358-2-M-6-M 337 PCT -4\0\0\1>S2-227-3-M-1-M 338 PCT -4\0\0\1>S2-227-3-M-2-M 339 PCT -4\0\0\1>S2-227-3-M-3-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 340 PCT -4\0\0\1>S2-227-3-M-4-M 341 PCT -4\0\0\1>S2-227-3-M-5-M 342 PCT -4\0\0\1 >S2-227 -3-M-6-M 343 PCT -4\0\0\1 >S2-227 -4-M-1-M 344 PCT -4\0\0\1 >S2-227 -4-M-2-M 345 PCT -4\0\0\1>S2-227-4-M-3-M 346 PCT -4\0\0\1>S2-227-4-M-4-M 34 7 PCT -4\0\0\1 >S2-227 -4-M-5-M 348 PCT -4\0\0\1 >S2-227 -4-M-6-M 349 PCT -4\0\0\1>S2-1584-1-M-1-M 350 PCT -4\0\0\1>S2-1584-1-M-2-M 351 PCT -4\0\0\1 >S2-1584-1-M-3-M 352 PCT -4\0\0\1 >S2-1584-1-M-4-M 353 PCT -4\0\0\1 >S2-1584-1-M-5-M 354 PCT -4\0\0\1>S2-1584-1-M-6-M 355 PCT -4\0\0\1>S2-1584-4-M-1-M 356 PCT -4\0\0\1 >S2-1584-4-M-2-M 357 PCT -4\0\0\1 >S2-1584-4-M-3-M 358 PCT -4\0\0\1>S2-1584-4-M-4-M 359 PCT -4\0\0\1>S2-1584-4-M-5-M 360 PCT -4\0\0\1>S2-1584-4-M-6-M 361 PCT -4\0\0\1>S2-2197-3-M-1-M 362 PCT -4\0\0\1 >S2-2197-3-M-2-M 363 PCT -4\0\0\1>S2-2197-3-M-3-M 364 PCT -4\0\0\1 >S2-2197 -3-M-4-M 365 PCT -4\0\0\1>S2-2197-3-M-5-M 366 PCT -4\0\0\1>S2-2197-3-M-6-M 367 PCT -4\0\0\1>S2-2145-5-M-1-M 368 PCT -4\0\0\1 >S2-2145-5-M-2-M 369 PCT -4\0\0\1>S2-2145-5-M-3-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 10 Oryzica Sabana 6 5 5 5 1 5 7 1 1 5 1 1 5 1 1 7 1 1 5 1 1 5 1 1 7 1 1 5 1 1 5 1 1 7 2 5 1 1 3 3 4 3 1 1 5 2 3 5 1 1 5 1 1 3 1 3 1 5 2 5 5 5 1 5 2 1 5 1 1 5 2 5 1 2 5 2 3 5 2 3 5 2 4 5 3 4 5 2 3 5 2 2 3 4 4 5 2 3 3 3 4 5 2 3 5 2 4 5 2 5 1 2 5 1 5 5 1 2 5 2 5 1 2 3 3 3 7 1 1 5 2 3 3 3 3 78 79 78 79 85 71 71 79 75 74 77 79 80 82 71 85 70 91 71 76 71 71 75 75 70 89 75 78 79 88 89 88 89 88 88 85 83 84 81 80 83 76 83 83 77 79 82 85 85 75 90 86 1 1 3 3 1 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 5 3 5 3 3 5 1 3 1 1 3 1 1 3 3 1 3 1 3 3 3 1 1 1 3 1 3 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 3 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 Linea 30 5 1 1 75 5 1 3 1 Oryzica Sabana 1 O 5 3 3 91 5 1 1 1 88 5860370 370 PCT -4\0\0\1 >52-2145-5-M-4-M 3 2 85 3 1 89 5860371 371 PCT -4\0\0\1 >52-2145-5-M-5-M 5 1 2 85 3 1 90 5860372 372 PCT -4\0\0\1 >52-2145-5-M-6-M 3 1 2 86 3 1 91 5860373 373 PCT -4\0\0\1 >52-1 038-1-M-1-M 5 1 2 86 3 1 1 92 5860374 374 PCT -4\0\0\1 >52-1 038-1-M-2-M 5 1 2 86 3 1 1 93 5860375 375 PCT -4\0\0\1 >52-1 038-1-M-3-M 5 1 2 86 3 1 1 1 94 5860376 376 PCT -4\0\0\1 >52-1 038-1-M-4-M 5 1 2 86 3 1 1 1 95 5860377 377 PCT -4\0\0\1 >S2-1 038-1-M-5-M 5 1 1 87 3 1 1 1 96 5860378 378 PCT -4\0\0\1 >52-1 038-1-M-6-M 5 2 2 84 3 1 1 1 97 5860379 379 PCT -4\0\0\1 >52-41-1-1,1-M 5 85 3 1 1 1 98 5860380 380 PCT -4\0\0\1>52-41-1-1,2-M 5 1 2 85 3 1 1 99 5860381 381 PCT -4\0\0\1 >52-41-1-1 ,3-M 5 3 3 86 3 1 3 1 100 5860382 382 PCT -4\0\0\1>52-41-1-1-4-M 5 1 1 89 3 1 1 1 101 5860383 383 PCT -4\0\0\1 >52-41-1-1-5-M 5 2 2 88 3 1 1 102 5860384 384 PCT -4\0\0\1 >52-41-1-1-6-M 5 2 2 86 3 1 1 1 103 5860385 385 PCT -4\0\0\1 >52-41-2-1-1-M 5 2 88 3 1 1 1 104 5860386 386 PCT -4\0\0\1 >52-41-2-1-2-M 5 1 2 88 3 1 1 1 105 5860387 387 PCT -4\0\0\1 >52-41 -2-1-3-M 5 1 2 89 3 1 1 106 5860388 388 PCT -4\0\0\1 >52-41-2-1-4-M 5 2 89 3 1 1 107 5860389 389 PCT -4\0\0\1 >52-41-2-1-5-M 5 2 89 3 1 108 5860390 390 PCT -4\0\0\1>52-41-2-1-6-M 5 1 2 89 3 1 1 109 5860391 391 PCT -4\0\0\1 >52-41-2-2-1-M 5 1 2 89 3 1 3 1 110 5860392 392 PCT -4\0\0\1 >52-41-2-2-2-M 7 1 1 90 3 1 1 1 111 5860393 393 PCT -4\0\0\1 >52-41-2-2-3-M 7 1 77 3 112 5860394 394 PCT -4\0\0\1 >52-41-2-2-4-M 7 2 1 71 3 1 1 113 S860395 395 PCT -4\0\0\1 >52-41-2-2-5-M 7 1 1 79 3 1 1 1 114 5860396 396 PCT -4\0\0\1 >52-41 -2-2-6-M 5 2 1 76 3 1 1 Vg = vigor; Bl 1 = leaf blast ; Bl 2 = leaf blast ; Fl = flowering; LSc = leaf scald; BS = brown spot; NBI = neck blast; Gd = grain discoloration. Table 12. Selected lines in the population PCT -4\0\0\1>S2 La Libertad experimental station, 1998A Nbr. Field Nbr. Origin Pedigree Vg Bl Bl Fl LSc BS GD NBI 1998A 19978 1 2 50% 1 5860286 286 PCT -4\0\0\1 >52-41-1-M-4-M 5 2 1 76 3 1 1 1 2 5860288 288 PCT -4\0\0\1 >$2-41-1-M-6-M 5 1 76 3 1 1 3 $860303 303 PCT -4\0\0\1 >$2-1803-3-M-3-M 5 1 78 5 3 1 4 $860304 304 PCT -4\0\0\1 >52-1803-3-M-4-M 5 1 77 3 1 1 5 $860319 319 PCT -4\0\0\1>52-2324-4-M-1-M 3 1 1 71 3 1 1 1 6 $860358 358 PCT -4\0\0\1 >52-1584-4-M-4-M 3 4 4 83 3 1 1 1 7 $860359 359 PCT -4\0\0\1 >$2-1584-4-M-5-M 5 2 3 84 3 1 1 8 $860360 360 PCT -4\0\0\1 >$2-1584-4-M-6-M 3 3 4 81 3 1 1 1 9 $860363 363 PCT -4\0\0\1 >52-2197 -3-M-3-M 5 1 2 76 3 1 1 1 10 $860366 366 PCT -4\0\0\1 >52-2197 "3-M-6-M 5 1 77 3 1 1 1 11 $860376 376 PCT -4\0\0\1 >$2-1 038-1-M-4-M 5 1 2 86 3 1 1 12 $860378 378 PCT -4\0\0\1 >$2-1 038-1-M-6-M 5 2 2 84 3 1 13 5860393 393 PCT -4\0\0\1 >$2-41-2-2-3-M 7 1 77 3 1 1 14 5860394 394 PCT -4\0\0\1 >$2-41-2-2-4-M 7 2 1 71 3 1 1 1 Vg = vigor; Bl 1 = leaf blast ; Bl 2 = leaf blast ; Fl = flowering; LSc = leaf scald; BS = brown spot; NBI = neck blast; Gd = grain discoloration. Table 13. S61ine evaluation of the population PCT -4\0\0\1 (selection of 53 plants at PES, 19969) La Libertad experimental station, 1998A Nbr. Field Nbr. Orfgin Pedlgree Vg 81 81 Fl LSc 85 GD N81 1998A 19978 1 2 50% 1 5860379 379 PCT -4\0\0\1 >52-41-1-1-1-M 5 1 1 85 3 2 5860380 380 PCT -4\0\0\1 >52-41-1-1-2-M 5 1 2 85 3 3 5860381 381 PCT -4\0\0\1>52-41-1-1-3-M 5 3 3 86 3 4 5860382 382 PCT -4\0\0\1 >52-41-1-1-4-M 5 1 1 89 3 5 5860383 383 PCT -4\0\0\1 >5 2-41-1-1-5-M 5 2 2 88 3 6 5860384 384 PCT -4\0\0\1 >52-41-1-1-6-M 5 2 2 86 3 7 5860385 385 PCT -4\0\0\1 >52-41-2-1-1-M 5 1 2 88 3 8 5860386 386 PCT -4\0\0\1 >52-41-2-1 -2-M 5 1 2 88 3 9 5860387 387 PCT -4\0\0\1 >52-41-2-1-3-M 5 1 2 89 3 10 5860388 388 PCT -4\0\0\1 >52-41-2-1-4-M 5 1 2 89 3 11 5860389 389 PCT -4\0\0\1 >52-41-2-1-5-M 5 1 2 89 3 12 5860390 390 PCT -4\0\0\1>52-41-2-1-6-M 5 1 2 89 3 13 5860391 391 PCT -4\0\0\1 >52-41-2-2-1-M 5 1 2 89 3 14 5860392 392 PCT -4\0\0\1 >52-41-2-2-2-M 7 1 90 3 15 5860393 393 PCT -4\0\0\1 >52-41-2-2-3-M 7 1 77 3 16 5860394 394 PCT -4\0\0\1 >52-41-2-2-4-M 7 2 1 71 3 17 5860395 395 PCT -4\0\0\1 >52-41-2-2-5-M 7 1 1 79 3 18 5860396 396 PCT -4\0\0\1 >52-41-2-2-6-M 5 2 1 76 3 Vg = vigor; 81 1 = leaf blast ; 81 2 = leaf blast ; Fl = flowerlng; LSc = leaf scald; 85 = brown spot; N81 = neck blast; Gd = grain discoloration. Table 14. 56 fines selected from the population PCT -4\0\0\1 (selection of S3 plants at PES, 19969) La Libertad experimental station, 1998A 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Nbr. Fleld Nbr. 1998A Orlgln Pedlgree 19978 Vg 81 81 Fl LSc 85 GD N81 1 2 50% 1 5860393 2 5860394 393 PCT -4\0\0\1 >52-41-2-2-3-M 71 773111 394 PCT -4\0\0\1 >52-41-2-2-4-M 7 2 71 3 1 1 Vg = vigor; 81 1 = leaf blast ; 81 2 = leaf blast ; Fl = flowerlng; LSc = leaf scald; 8S = brown spot; N81 = neck blast; Gd = grain discoloration. 1 1 t 1 Table 15. INGER- LAC: VIOAL Nursery for Acld Solls, 1998 Cons. 1 2 3 4 5 6 7 6 9 10 11 12 13 14 15 16 17 16 19 20 21 22 23 24 25 26 27 28 29 ~ 31 Whlte Pedlgree Vg. Bl Bl AC NBI Fl LSc BS VHB Gd Ht G.T W.B. S.T Amyl. rice 1 2 (00%) (%) (%) CNA-IRAT-5\SA\0\3>1 27-2-M-1-M-2 3 1 2 1 1 63 1 1 1 1 104 1 0.6 L 29 80.14 CNA-IRAT -A\SA '0.3>1-M-2-M-1-M-1 5 1 1 1 1 66 1 1 9 1 102 H 0.8 L 22 69.40 CNA-IRAT -A\SA '0.3>1-M-2-M-1-M-2 3 1 1 1 1 66 1 1 9 1 99 H 1.2 L 22 73.88 CNA-IRAT -A\SA '0.3>1 -M-2-M-1-M-5 3 1 1 1 1 66 1 1 9 1 106 H 2.2 L 20 66.72 CNA-IRAT -A\SA '0.3> 1-M-2-M-2-M-1 3 1 1 1 1 63 1 1 9 1 112 H 2.0 L 22 71 .29 CNA-IRAT-A\SA'D\3>1-M-2-M-2-M-4 3 1 1 1 1 66 1 1 9 1 105 H 1.2 L 24 66.67 CNA-IRAT-A\SAVJ\3>1-M-2-M-3-M-2 3 1 1 1 1 66 1 1 9 1 112 H 1.6 L 20 67.58 CNA-IRAT-A\SA'D\3>1-M-2-M-3-M-4 5 1 1 1 1 66 1 1 9 1 113 H 1.0 L 23 70.20 CNA-IRAT-A\SA'D\3>1-M-2-M-3-M-5 5 1 1 1 1 66 1 1 9 1 112 H 1.8 L 22 71.64 CNA-IRA T-A\SA'0.3>1-M-2-M-4-M-2 3 1 1 1 1 66 3 1 9 1 97 H 1.6 L 23 72.99 CNA-IRAT-A\SA '0.3>1-M-2-M-4-M-3 1 1 1 1 1 66 3 1 9 1 95 H 1.2 L 21 72.15 CNA-IRAT-A\SA '0.3>1-M-2-M-4-M-4 3 1 1 1 1 69 1 1 9 1 95 H 1.2 L 22 74.24 CNAx3606-6-1-2-2-1-M-M 3 1 2 1 1 66 1 1 9 1 112 - 0.1 EL CANx3619-3-1 -B-2-M-M 3 3 4 1 1 86 3 1 9 1 102 . 1 1.2 L CNAx4754-SO-B-M-7 -M-M 3 1 2 1 1 66 1 1 9 1 97 - 0.6 EL C NAx4754-128-B-M-4-M 3 1 1 1 1 74 1 1 9 1 95 H 1.0 L CNAx4754-61-B-M-20-M 3 1 1 1 1 91 3 1 9 1 104 H 0 .4 L CT1348(}.M-16-1-M-M 3 1 1 1 1 86 3 1 3 63 - - - CT13480-M-10-1 -M-M 1 1 2 1 1 94 5 1 3 66 B 0.6 L CT13468-M-9-2-M-M 1 1 1 1 1 85 3 1 3 69 1 1.2 L CT13603-M-3-1 -M-M 1 1 3 1 1 85 3 3 3 68 Bl 0.8 L CT13603-M-13-1-M-M 3 2 2 1 3 86 5 3 5 76 B 0.2 L CT13603-M-18-1-M-M 3 1 1 1 1 85 5 1 5 78 1 0.6 L CT13603-M-18-2-M-M 1 1 1 1 1 86 5 3 5 76 - 1.0 L CT13603-M-18-3-M-M 1 1 1 1 1 86 5 3 5 74 - 2.0 EL CIRAD409 1 1 1 1 1 62 3 1 1 91 1 1.2 L 24 71 .06 CIRAD410 3 3 3 1 1 67 3 5 1 67 1 3.8 M 24 85.20 CIRAD 411 5 3 3 1 1 70 3 3 1 102 Hl 2.8 L 17 67.25 ORYZICA SABANA 6 3 3 4 1 1 82 5 1 1 106 1 2.2 L 26 73.04 ORYZICA SABANA 1 O 3 2 1 1 1 86 3 1 1 106 H 1.2 L 23 71 .53 lRAT 216 4 - 7 2 1_ c___.1___ _67_ ~-~3 ~ --- - --- - - - - ---- Vg = vigor; Bl 1 = leafblast ; Bl 2 = leafblast ; AC = acid soil reaction; Fl = flowering; LSc = leaf scald; BS = brown spot; NBl = neck blast; Gd = grain discoloration; Sel. = number selected; Ht = height (cm). G.T. = Gelatinization temperature; W.B. = white belly; Amyl. = amylose content. _____ ......_.. -~--.. --___ .._ ........... - ........ ~-........ -... ~ .. --~ Head rice (%) 66.76 1 62.95 67.10 59.61 63.15 58.~ 58.58 63.50 66.36 66.89 67.10 67.95 66.6J 66.86 56.32 56.67 61 .73 ~--- - ~-... Table 16. INGER LAC, VIOAL Nursery for SUELOS ACIDOS- Evaluation in Colombia La Libertad experimental station, 1998A Nbr. Fleld Nbr. Orlgln Pedlgree Vg Ht Bl Bl 1 2 Fl LSc BS GD NBI Hum Rto. 1998A 19978 1 S870001 2 S870002 3 S870003 4 S870004 5 S870005 6 S870006 7 5870007 8 5870008 9 S870009 10 S870010 11 5870011 12 S870012 13 5870013 14 5870014 15 5870015 16 S870016 17 8870017 18 S870018 19 S870019 20 21 22 23 S870020 24 S870021 25 S870022 26 S870023 27 5870024 28 S870025 29 8870026 30 S870027 31 5870028 32 S870029 33 5870030 1 CNA-IRAT-5\SA\0\3>127-2-M-1-M-2 2 CNA-IRAT-A\SA\0\3>1-M-2-M-1-M-1 3 CNA-IRAT-A\SA\0\3>1-M-2-M-1-M-2 4 CNA-IRAT-A\SA\0\3>1-M-2-M-1-M-5 5 CNA-IRAT-A\SA\0\3>1-M-2-M-2-M-1 6 CNA-IRAT-A\SA\0\3>1-M-2-M-2-M-4 7 CNA-IRAT-A\SA\0\3>1-M-2-M-3-M-2 8 CNA-IRAT-A\SA\0\3>1-M-2-M-3-M-4 9 CNA-IRAT-A\SA\0\3>1-M-2-M-3-M-5 10 CNA-IRAT-A\SA\0\3>1-M-2-M-4-M-2 11 CNA-IRA T-A\SA\0\3>1-M~2-M-4-M-3 12 CNA-IRAT-A\SA\0\3>1-M-2-M-4-M-4 13 CNAx3608-6-1-2-2-1-M-M 14 CANx3619-3-1-B-2-M-M 15 CNAx4754-80-B-M-7-M-M 16 CNAx4754-128-B-M-4-M 17 CNAx4754-61-B-M-20-M 18 CT 13480-M-16-1-M-M 19 CT 13480-M-1 0-1-M-M Oryzica Sabana 6 Linea 30 Oryzica Sabana 1 O 20 CT13488-M-9-2-M-M 21 CT13503-M-3-1-M-M 22 CT13503-M-13-1-M-M 23 CT13503-M-18-1-M-M 24 CT13503-M-18-2-M-M 25 CT13503-M-18-3-M-M 26 CIRAD 409 27 CIRAD 410 28 CIRAD 411 29 ORYZICA SABANA 6 30 ORYZICA SABANA 10 5 1 7 1 7 97 1 3 2 2 7 97 2 2 7 95 2 1 7 98 1 7 100 1 7 97 1 1 7 113 1 1 7 100 1 1 7 112 2 1 7 96 2 1 7 88 2 1 7 92 3 3 7 108 2 1 7 99 2 1 7 110 1 1 5 104 1 1 7 112 1 3 7 85 3 3 5 74 1 1 5 112 2 2 5 96 1 5 97 1 5 80 1 1 7 81 1 7 82 1 1 7 81 1 1 5 90 1 1 5 85 1 1 5 87 2 5 103 3 3 5 85 2 2 Vg = vigor; Bl 1 = leaf blast ; Bl 2 = leaf blast ; Fl = ftowering; LSc = leaf scald; BS = brown spot; 50% 66 71 75 74 71 74 74 73 77 81 80 81 74 94 75 81 94 89 92 86 73 87 86 88 90 90 88 90 71 71 75 89 89 NBI = neck blast; Gd = grain dlscoloratlon. Ht = plant height; Hum = grain humidity; Rdo = grain yield. 1 1 1 1 1 1 1 1 1 1 1 3 3 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 3 3 1 1 3 1 1 1 1 3 1 5 3 1 5 1 1 5 3 1 3 1 3 3 1 3 3 3 3 3 3 3 3 1 3 3 3 1 5 1 3 1 1 3 1 1 3 1 1 3 3 3 3 3 2 5 1 % 1 16.3 1 15.9 16.3 1 15 1 15.4 1 15 1 14.3 1 14.3 1 15 1 15 1 14.3 1 15.4 Kg/ha 1433.68 1015.52 1127.85 1054.52 1410.50 1189.85 973.52 1033.69 1033.99 980.01 854.70 1145.66 1 15.4 1582.28 1 16.3 877.80 15.4 1614.06 1 15 1975.60 1 17.3 1811 .93 16.3 1965.60 14.3 1245.64 1 17.3 2190.66 1 16.9 2202.74 1 16.9 1426.38 1 15.9 1820.04 3 16.9 2047.77 3 14.3 2275.11 3 16.3 1918.06 1 15.4 1600.44 1 14.3 2200.37 1 16.3 1706.19 1 15.9 1148.66 15.9 999.35 1 17.7 1799.48 1 20.1 1032.70 Table 17. Evaluationof Advanced lines from the population PCT-3 La Libertad experimental station, 1998A Nbr. Field Nbr. Orlgin Pedlgree Vg Bl Bl Fl 1998A 19978 1 2 50% 1 S880001 1 CT13480-M-1 0-1-M-M-1 5 2 2 91 2 S880002 2 CT13480-M-1 0-1-M-M-2 5 3 4 93 3 S880003 3 CT 13503-M-18-1-M-M-1 5 1 86 4 S880004 4 CT13503-M-18-2-M-M-1 5 2 85 LSc BS GD 3 5 1 3 3 1 3 5 3 Vg = vigor; 81 1 = leaf blast : 81 2 = leaf blast : Fl = flowering; LSc = leaf scald; 8S = brown spot; N81 = neck blast; Gd = grain discoloration; Sel = mass selection. NBI Sel. 1 M 3M 1 M 5M Tabl18. S21ines selected for recombination from the population PCT -4\SA\1\1 after statistical analysis of the Augmented Designs trial LA Libertad experimental station, 1998A Nbr. Origin Origin Pedigree Nbr. Origin Origin Pedigree 1998A 19976 1998A 19976 1 890011 11 PCT-4\SA\1\1>162 31 890098 98 PCT -4\SA\1\1>1145 2 890015 15 PCT-4\SA\1\1>193 32 890099 99 PCT-4\SA\1\1>1155 3 890017 17 PCT -4\SA\1\1>195 33 890101 101 PCT-4\SA\1\1 >1156 4 890019 19 PCT -4\SA\1\1>223 34 890103 103 PCT-4\SA\1\1 >1199 5 890020 20 PCT -4\SA\1\1 >230 35 890105 105 PCT-4\SA\1\1>1206 6 890021 21 PCT -4\SA\1\1>236 36 890109 109 PCT-4\SA\1\1>1259 7 890022 22 PCT-4\SA\1\1>241 37 890110 110 PCT -4\SA\1\1>1260 8 890029 29 PCT -4\SA\1\1 >305 38 890119 119 PCT-4\SA\1\1>1358 9 890038 38 PCT-4\SA\1\1>428 39 890121 121 PCT-4\SA\1\1>1377 10 890047 47 PCT-4\SA\1\1>503 40 890122 122 PCT-4\SA\1\1>1389 11 890050 50 PCT -4\SA\1 \1>542 41 890123 123 PCT -4\SA\1\1>1392 12 890058 58 PCT -4\SA\1 \1>631 42 890124 124 PCT-4\SA\1\1>1414 13 890073 73 PCT -4\SA\1\1>881 43 890125 125 PCT-4\SA\1\1>1443 14 890075 75 PCT -4\SA\1\1>895 44 890126 126 PCT -4\SA\1\1>1472 15 890076 76 PCT-4\SA\1\1>910 45 890128 128 PCT -4\SA\1 \1>1475 16 890080 80 PCT -4\SA\1\1 >954 46 890129 129 PCT-4\SA\1\1>1479 17 890082 82 PCT -4\SA\1\1>975 47 890131 131 PCT-4\SA\1\1>1486 18 890083 83 PCT-4\SA\1\1>982 48 890132 132 PCT-4\SA\1\1>1512 19 890084 84 PCT-4\SA\1\1>1013 49 890133 133 PCT-4\SA\1\1>1538 20 890086 86 PCT-4\SA\1\1>1034 50 890134 134 PCT-4\SA\1\1>1549 21 890087 87 PCT -4\SA\1\1>1036 51 890135 135 PCT -4\SA\1\1>1559 22 890088 88 PCT-4\SA\1\1>1044 52 890136 136 PCT-4\SA\1\1>1566 23 890089 89 PCT -4\SA\1 \1>1047 53 890140 140 PCT -4\SA\1\1>1632 24 890090 90 PCT -4\SA\1\1>1048 54 890143 143 PCT-4\SA\1\1>1666 25 890091 91 PCT -4\SA\1\1>1108 55 890144 144 PCT-4\SA\1\1>1667 26 890092 92 PCT-4\SA\1\1>1116 56 890145 145 PCT-4\SA\1\1>1689 27 890093 93 PCT -4\SA\1\1>1127 57 890147 147 PCT-4\SA\1\1>1701 28 890094 94 PCT -4\SA\1\1>1128 58 890148 148 PCT-4\SA\1\1>1702 29 890095 95 PCT -4\SA\1 \1>1135 59 890168 168 PCT-4\SA\1\1>1915 30 890097 97 PCT-4\SA\1\1>1138 60 890170 170 PCT-4\SA\1\1>1919 Tabl19. SO fertile plants selected in the population PCT-4\SA\2\1 La Libertad experimental stationEstación Experimental La Libertad 1998A Nbr. Pedigree 1 PCT-4\SA\2\1>38 2 PCT-4\SA\2\1>43 3 PCT-4\SA\2\1>44 4 PCT -4\SA\2\ 1 >69 5 PCT-4\SA\2\1>113 6 PCT-4\SA\2\1>128 7 PCT -4\SA\2\ 1 > 172 8 PCT-4\SA\2\1>190 9 PCT-4\SA\2\1>192 10 PCT-4\SA\2\1>275 11 PCT -4\SA\2\ 1 >282 12 PCT -4\SA\2\1 >287 13 PCT-4\SA\2\1>290 14 PCT-4\SA\2\1>310 15 PCT-4\SA\2\1>317 16 PCT-4\SA\2\1>331 17 PCT -4\SA\2\ 1 >333 18 PCT-4\SA\2\1>335 19 PCT -4\SA\2\1>375 20 PCT -4\SA\2\ 1 >438 21 PCT -4\SA\2\1>473 22 PCT -4\SA\2\ 1 >4 77 23 PCT -4\SA\2\ 1 >482 24 PCT-4\SA\2\1>521 25 PCT-4\SA\2\1>529 26 PCT-4\SA\2\1>589 27 PCT-4\SA\2\1>617 28 PCT -4\SA \2\1 >626 29 PCT -4\SA\2\ 1 >657 30 PCT -4\SA \2\1 >664 31 PCT-4\SA\2\1>676 32 PCT -4\SA \2\1 >677 33 PCT -4\SA \2\1 >697 34 PCT -4\SA\2\ 1 > 731 35 PCT-4\SA\2\1>734 36 PCT-4\SA\2\1>764 37 PCT -4\SA \2\1 > 768 38 PCT-4\SA\2\1>779 Nbr. Pedigree 39 PCT -4\SA\2\1 >786 40 PCT -4\SA\2\1 >792 41 PCT -4\SA\2\1>805 42 PCT -4\SA\2\1 >831 43 PCT -4\SA\2\1 >855 44 PCT -4\SA\2\1 >979 45 PCT -4\SA\2\ 1 >1 020 46 PCT -4\SA\2\1 >1158 47 PCT -4\SA\2\1 >1181 48 PCT -4\SA\2\ 1 >1190 49 PCT-4\SA\2\1>1196 50 PCT-4\SA\2\1>1198 51 PCT -4\SA\2\1>1225 52 PCT -4\SA\2\1 >1234 53 PCT -4\SA\2\1 >1235 54 PCT -4\SA\2\1>1238 55 PCT -4\SA\2\1>1270 56 PCT -4\SA\2\1>1300 57 PCT -4\SA\2\ 1 >1306 58 PCT -4\SA\2\1>1326 59 PCT -4\SA\2\ 1 >1343 60 PCT -4\SA\2\1>1416 61 PCT-4\SA\2\1>1417 62 PCT-4\SA\2\1>1492 63 PCT -4\SA\2\1 >1513 64 PCT-4\SA\2\1>1559 65 PCT -4\SA\2\1>1564 66 PCT -4\SA\2\ 1 >1605 67 PCT -4\SA\2\1>1615 68 PCT -4\SA\2\1>1669 69 PCT -4\SA\2\1>1699 70 PCT -4\SA\2\1>1728 71 PCT -4\SA\2\1>1794 72 PCT -4\SA\2\1>1807 73 PCT -4\SA\2\1>1836 74 PCT -4\SA\2\1>1923 75 PCT -4\SA\2\1 >1985 Table 20. SO fertile plants selected in the population PCT-5\PHB\1\0,PHB\1 ,PHB\1 La Libertad experimental station, 1998A Nbr. Pedigree 1 PCT-\PHB\1\0,PHB\1 ,PHB\1 >126 2 PCT-\PHB\ 1 \O,PHB\ 1 ,PHB\ 1 >163 3 PCT-\PHB\1\0,PHB\1 ,PHB\1>192 4 PCT-\PHB\1\0,PHB\1 ,PHB\1>226 5 PCT-\PHB\ 1 \O,PHB\ 1 ,PHB\ 1 >230 6 PCT-\PHB\1\0,PHB\1 ,PHB\1 >242 7 PCT-\PHB\1\0,PHB\1 ,PHB\1 >258 8 PCT-\PHB\1 \0,PHB\1 ,PHB\1 >331 9 PCT-\PHB\1\0,PHB\1,PHB\1>338 10 PCT-\PHB\1\0,PHB\1,PHB\1>384 11 PCT-\PHB\1\0,PHB\1,PHB\1 >385 12 PCT-\PHB\1\0,PHB\1,PHB\1>439 13 PCT-\PHB\1\0,PHB\1,PHB\1 >444 14 PCT-\PHB\1 \0,PHB\1,PHB\1>515 15 PCT-\PHB\1\0,PHB\1 ,PHB\1 >579 Nbr. Pedigree 16 PCT -\PHB\ 1 \O,PHB\ 1 ,PHB\ 1 >590 17 PCT-\PHB\1\0,PHB\1,PHB\1>618 18 PCT-\PHB\1\0 ,PHB\1,PHB\1>619 19 PCT-\PHB\1\0,PHB\ 1,PHB\1>652 20 PCT-\PHB\1 \0,PHB\ 1,PHB\1>730 21 PCT-\PHB\1\0,PHB\1 ,PHB\1 >842 22 PCT-\PHB\1\0,PHB\1,PHB\1>1037 23 PCT-\PHB\1\0,PHB\ 1,PHB\1>1129 24 PCT-\PHB\1 \0,PHB\ 1,PHB\1>1341 25 PCT-\PHB\1\0,PHB\ 1,PHB\1>1591 26 PCT-\PHB\1\0,PHB\ 1,PHB\1>1651 27 PCT-\PHB\1\0,PHB\ 1 ,PHB\1 >1661 28 PCT-\PHB\1\0 ,PHB\1,PHB\1>1670 29 PCT-\PHB\1\0,PHB\ 1,PHB\1>1853 30 PCT-\PHB\1\0,PHB\1 ,PHB\1>1917 Table 21. SO fertile plants selected in the population PCT-A\PHB\1 \0,PHB\1,PHB\1 La Libertad experimental station 1998A Nbr. Pedigree 1 PCT-A\1\0,PHB\1,PHB\1>253 2 PCT-A\1\0,PHB\1,PHB\1>397 3 PCT-A\1\0,PHB\1 ,PHB\1>421 4 PCT-A\1\0,PHB\1,PHB\1>642 5 PCT-A\1\0,PHB\1,PHB\1>721 6 PCT-A\1\0,PHB\1 ,PHB\1>866 7 PCT-A\1\0,PHB\1,PHB\1>946 8 PCT-A\1\0,PHB\1,PHB\1>1058 9 PCT-A\1\0,PHB\1,PHB\1>1194 10 PCT-A\1\0,PHB\1,PHB\1>1302 11 PCT-A\1\0,PHB\1,PHB\1>1326 12 PCT-A\1\0,PHB\1,PHB\1>1347 Nbr. Pedigree 13 PCT-A\1 \0,PHB\1,PHB\1>1361 14 PCT-A\1\0,PHB\1,PHB\1>1447 15 PCT-A\1 \0,PHB\1,PHB\1>1631 16 PCT-A\1\0,PHB\1,PHB\1>1744 17 PCT-A\1 \0,PHB\1 ,PHB\1>1802 18 PCT-A\1\0,PHB\1 ,PHB\1>1827 19 PCT-A\1\0,PHB\1 ,PHB\1>1834 20 PCT-A\1\0,PHB\1 ,PHB\1>1850 21 PCT-A\1\0,PHB\1,PHB\1>1889 22 PCT-A\1\0,PHB\1,PHB\1>1933 23 PCT-A\1\0,PHB\1,PHB\1>1990 24 PCT-A\1\0,PHB\1,PHB\1>2007 Table 22. SO fertile plants selected in the population PCT-4\PHB\1\1 ,PHB\1 ,PHB\1 La Libertad experimental station, 1998A Nbr. Pedigree 1 PCT-4\PHB\1\1 ,PHB\1,PHB\1>135 2 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>191 3 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>227 4 PCT-4\PHB\1\1,PHB\1,PHB\1>228 5 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>262 6 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>300 7 PCT-4\PHB\1\1,PHB\1 ,PHB\1>329 8 PCT-4\PHB\1\1,PHB\1,PHB\1>336 9 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>359 10 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>443 11 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>455 12 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>472 13 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>479 14 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>507 15 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>509 16 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>541 17 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>572 18 PCT-4\PHB\1\1,PHB\1,PHB\1>583 19 PCT-4\PHB\1\1,PHB\1,PHB\1>584 20 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>585 21 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>587 22 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>603 23 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>614 24 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>650 25 PCT-4\PHB\1\1,PHB\1,PHB\1>692 26 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>816 27 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>869 Nbr. Pedigree 28 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>894 29 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1047 30 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1062 31 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1093 32 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1112 33 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1155 34 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1234 35 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1255 36 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1264 37 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1298 38 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1308 39 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1 >1335 40 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1373 41 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1376 42 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1377 43 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1382 44 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1 >1398 46 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1515 47 PCT -4\PHB\1\1,PHB\1 ,PHB\1>1545 48 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1652 49 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1697 50 PCT -4\PHB\1 \1 ,PHB\1 ,PHB\1>1734 51 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1735 52 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1 >1864 53 PCT -4\PHB\1 \1 ,PHB\1 ,PHB\1>1895 54 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1912 55 PCT -4\PHB\1\1 ,PHB\1 ,PHB\1>1945 Table 23. SO fertile plants selected in the population PCT-11\0\0\1 La Libertad experimental station, 1998A Nbr. Pedigree 1 PCT -11\0\0\1 >42 2 PCT-11\0\0\1>51 3 PCT-11 \0\0\1>52 4 PCT-11\0\0\1>73 5 PCT-11\0\0\1>84 6 PCT-11\0\0\1 >87 7 PCT-11\0\0\1 >110 8 PCT-11\0\0\1 >112 9 PCT-11\0\0\1>134 10 PCT-11 \0\0\1>137 11 PCT-11\0\0\1>150 12 PCT-11\0\0\1>155 13 PCT-11\0\0\1 >166 14 PCT-11\0\0\1>224 15 PCT-11\0\0\1>225 16 PCT-11\0\0\1>228 17 PCT-11\0\0\1>242 18 PCT-11\0\0\1>244 19 PCT-11\0\0\1>256 20 PCT-11\0\0\1>261 21 PCT-11\0\0\1>269 22 PCT-11\0\0\1>309 23 PCT-11\0\0\1>314 24 PCT-11\0\0\1>317 25 PCT-11\0\0\1 >375 26 PCT-11\0\0\1 >376 27 PCT-11\0\0\1>386 28 PCT -11\0\0\1 >405 29 PCT-11\0\0\1>438 30 PCT -11 \0\0\1 >493 31 PCT-11 \0\0\1>509 32 PCT-11\0\0\ 1 >513 33 PCT-11\0\0\1>516 34 PCT-11\0\0\1>522 35 PCT -11\0\0\1 >549 36 PCT -11\0\0\1 >566 37 PCT-11\0\0\1 >576 38 PCT-11\0\0\1>621 39 PCT-11\0\0\1 >657 40 PCT-11\0\0\1 >660 41 PCT-11\0\0\1>727 42 PCT-11\0\0\1>733 43 PCT-11\0\0\1>734 44 PCT-11\0\0\1>781 45 PCT-11\0\0\1>782 46 PCT-11\0\0\ 1 >801 47 PCT-11\0\0\1>810 48 PCT-11\0\0\1 >816 Nbr. Pedigree 49 PCT -11 \0\0\1 >831 50 PCT -11\0\0\1 >841 51 PCT-11\0\0\1>866 52 PCT-11\0\0\1>877 53 PCT -11\0\0\1 >903 54 PCT -11\0\0\1 >905 55 PCT -11 \0\0\1 >908 56 PCT-11\0\0\1>924 57 PCT-11\0\0\1>943 58 PCT -11 \0\0\1 >953 59 PCT-11\0\0\1>1013 60 PCT-11\0\0\1 >1050 61 PCT-11\0\0\1 >1 083 62 PCT-11 \0\0\1>1092 63 PCT-11\0\0\1 >1138 64 PCT-11\0\0\1>1184 65 PCT-11 \0\0\1>1196 66 PCT-11\0\0\1 >1199 67 PCT-11 \0\0\1>1308 68 PCT-11\0\0\1 >1310 69 PCT-11\0\0\1>1337 70 PCT-11\0\0\1>1372 71 PCT-11 \0\0\1>1384 72 PCT-11\0\0\1>1397 73 PCT-11\0\0\1 >1461 74 PCT-11\0\0\1>1486 75 PCT-11\0\0\1>1520 76 PCT-11\0\0\1>1526 77 PCT-11\0\0\1>1537 78 PCT-11\0\0\1>1600 79 PCT-11\0\0\1>1606 80 PCT-11\0\0\1>1627 81 PCT-11\0\0\1 >1628 82 PCT-11\0\0\1 >1629 83 PCT-11\0\0\1>1636 84 PCT-11\0\0\1 >1660 85 PCT-11\0\0\1>1670 86 PCT-11\0\0\1 >1681 87 PCT-11\0\0\1>1694 88 PCT-11\0\0\1 >1709 89 PCT-11\0\0\1>1777 90 PCT-11\0\0\1>1780 91 PCT-11\0\0\1>1781 92 PCT-11\0\0\1>1790 93 PCT-11 \0\0\1 >1814 94 PCT-11\0\0\1>1959 95 PCT-11\0\0\1 >1961 Table 24. Yield trial of 8 advanced lines La Libertad experimental station, 1998A Nbr. Pedigree Fl Ht Hum Rto 50% Cm % Kg/ha 1 Oryzica Sabana 6 85 99 17.7 2404.4 2 CIRAD409 66 88 20.1 2891 .26 3 CIRAD 410 67 81 18.6 2228.4 4 CIRAD 411 72 85 21 1779.18 5 Oryzica Sabana 1 O 89 102 20.1 1791.55 6 CNA IRAT-A\SA\0\3>1-M-2-M-1-M-1 64 102 16.9 1675.04 7 CNA IRAT-A\SA\0\3>1-M-2-M-1-M-1 69 95 18.2 1591.77 8 CNA IRAT-A\SA\0\3>1-M-2-M-3-M-4 68 100 18.2 1637.58 Fl = flowering; Hum = grain humldity; Rdo = grain yield Table 25. Selected lines from the conventional breeding project La Libertad experimental station, 1998A Nbr. Field Nbr. Origin Pedlgree Vg Bl Bl FJ LSc BS GD NBI 1998A 19978 1 2 50% 1 8800017 17 CT9899-12-6-3P-1-4-M 5 3 4 82 3 1 1 1 2 8800019 19 CT9907 -5-3-1 P-3-1-M 5 3 4 79 3 3 1 1 3 8800022 2.2 CT11632-3-3-M 5 2 3 93 3 1 1 4 8800026 26 CT11231-2-2-1-3-M 5 3 4 79 3 1 1 5 8800031 31 CT11891-3-3-3-M 5 3 3 70 3 1 6 8800040 40 CT13366-8-2-M 5 2 1 70 5 1 7 8800043 43 CT13366-9-3-M 5 3 3 77 5 1 8 8800044 44 CT13366-9-5-M 5 2 3 80 5 1 9 8800059 59 CT13370-2-1-M 5 1 2 81 3 1 10 8800064 64 CT13370-3-4-M 5 2 3 84 3 1 1 11 8800098 98 CT13371-5-1-M 5 1 2 79 3 1 1 12 8800120 120 CT 13382 -8-3-M 3 2 2 82 3 1 13 8800125 125 CT13382-9-M 5 2 2 83 3 1 14 8800127 127 CT13569-5-1-M 5 2 4 86 3 1 15 8800129 129 CT13569-5-5-M 5 2 2 84 3 1 1 16 8800130 130 CT13569-5-6-M 5 2 2 83 3 1 1 17 8800132 132 CT13569-8-1-M 5 2 3 81 3 1 1 3 18 8800133 133 CT13569-8-2-M 5 2 3 80 3 19 8800134 134 CT13570-1-3-M 5 1 3 81 3 1 20 8800135 135 CT13570-3-2-M 5 1 3 83 3 1 1 21 8800136 136 CT13570-3-3-M 5 2 3 83 3 1 1 22 8800137 137 CT13570-13-4-M 5 2 2 80 3 1 1 23 8800142 142 CT13571 -12-3-M 5 3 3 85 1 24 8800146 146 CT13572-3-3-M 7 3 4 71 Vg =vigor; Bl 1 = leaf blast ; Bl 2 = leaf blast ; Fl = flowering; LSc = leaf scald; BS = brown spot NBI = neck blast; Gd = grain discoloration Table 26. Selected lines in the introduction trial from WARDA (O.sativa x O. glaberrima) La Libertad experimental station, 1998A Nbr. Field Nbr. Origin Pedlgree Vg Ht 81 81 Fl LSc 85 GD NBI Hum Rto. 1998A 19978 1 2 50% o¡. grs 1 S810004 4 WAB450-1-B-P-82-2-1 7 98 1 1 77 1 1 18.2 510.3 2 S810006 6 WAB450-II-1-2-P50-HB 5 85 2 2 74 3 1 1 15.0 233.2 3 S810011 11 W AB450-I-B-P-78-HB 7 94 2 70 1 1 1 15.4 363.6 4 S810017 17 WAB450-I-B-P-57 -3-1 7 104 2 79 1 1 1 15.9 322.4 5 S810021 21 WAB450-I-B-P-133-HB 5 92 2 2 75 1 1 1 14.3 408.7 6 S810022 22 WAB450-I-B-P-6-2-1 5 89 2 2 78 1 1 1 . 15.4 417.2 7 S810079 79 W AB450-16-2-BL 1-DR2 7 71 3 2 71 1 1 15.9 325.6 8 S810082 82 WAB450-16-2-BL 1-DV1 7 70 2 1 70 1 15.0 389.9 Vg = vigor; 81 1 = leaf blast ; 81 2 = leaf blast ; Fl = ftowering; LSc = leaf scald; 8S = brown spot; N81 = neck blast; Gd = grain dlscoloration, Ht = plant height; Hum = grain humidity; Rdo = grain yield. APPENDIX l. RICE PROJECT IP-4. Breakdown Structure and Logical Framework . Project IP-4. lmproved Rke Germplasm ror Latin Amerka and tbe Carlbbean Project Goal To lmprove the nutritíonal and economic well-being of rice growers and low lncome consumers In Latín America and the Caribbean lhrough sustainable increases in rice production and productivlty Project Purpose To increase genetic diversity and enhance gene pools for higher, more stable ylelds wilh lower unlt productlon costs and reduce environmental hazards 1 1 1 1 Physlological basls for rice traits Rice pests and genetícs of resistance Project priorities and research capacities Enhanced Gene Pools understood characterized enhanced - Rice improvement for upland and - Weed control enhanced by lhe use - Monitoring changes in genetíc - Analysis of natíonal rice samples in lowland using recurren! selection and of new genotypes and practicas structure and virulence diversity of Colombia conventíonal breeding - ldentificatíon of rice cultlvars wilh the rice blast pathogen - Creatíon of a network of rice - Evaluatíon of savannas upland rice tolerance to submergence - Characterizatíon of rice germplasm economics in Latín America lines in Latín American countries - ldentíficatíon of rice cultívars for blast resistance (RE CAL) - Populatíon maintenance through exhibitíng hlgh weed competltion - Genetlcs and dissection of blast - Collaboratlon with IFPRI in varietal recombination - Characterlzatlon of useful tralts In resistance genes using molecular adoptlon studles in Latín America - Registration of new populatíons new plant type derived populations markers and the Caribbean - Distributíon of breeding populatíons to - lmprovlng methods for developin - FLAR breeding and crop LAC countries durable blast resistance management activitles in Latín - Conventíonal and recurren! selectíon - Characterlzation of partial reslstance Amerlca and the Caribbean breeding for hillsides upland rice to blast in existíng germplasm (training) - ldentification and selectíon for useful - Characterizatíon of resistance to - Germplasm characterization for traits In wild rices with the aid of RHBV and Tagosodes oryzicolus yield, milling quallty, blast molecular markers. lmprovement of - Maintalning and establishing colonles reslstance, iron toxicity tolerance, yield potential in interspecific crosses of T. oryzicolus RHBV resistance, cold tolerance by - Evaluation of interspecific populations. - Epidemiology for RHBV FLAR lntrogression of new plant type lnto - Control of RHBV lhrough - Crosses and evaluatíon of LAC's gene pols nudeoproteln mediated cross segregating populations by FLAR - Use of anther and in vitro culture and protectíon in transgenic rice and country members somadonal variation for enhancement - Physical, chemical and moleculars - FLAR lnteractions with of gene pools characterization of the rice stripe CIRAD/CIAT/IRRI necrosis virus - Collaboration with Forest Margins project for the development of lmproved upland rice cultivars for the Peruvian selva LOGICAL FRAMEWORK • PROJECT IP-4 Proiect Titl -· ····t'·-· - d Rice G -···'t''- -· ·· ·-· LatinAm · -··--· d the Caribbean Proiect Manaaer: F . doCo -·. ·-· ·-- --·. -- Narrativa Summarv Measurable lndicators Means of Verification lmportantAssumptions Goal To improve the nutritional and economic well-being of lmproved access of rice growers and consumers to standard Natlonal statistics on Donors, govemments and NARS continuad goocls and services. agricultura and development interest in sustainable increase in rice rice growers and low income consumers in Latin of LAC. production. America and the Caribbean through sustainable Reduction in pesticida use and increase yield average/ha . Rice productlon statlstics. increases in rice production and productivity lncrease in the number of ha plantad with new cultivars. Purpose Evaluatlons of yield potentlal of F2BC2, end 1998. To increase genetic divers\ty and enhance gene pools lncreased use of improved populations from recurren! selection by Database on seed exchange. lmprovedldiversed populations are for higher, more stable yields with lower unit production NARS at the end of 1997. Project, CIAT and NARS adopted/used by NARS; policies favor adoption. costs and reduce environmental hazards Rice lines selected with desired gene traits. annual reports. Farmers are willing to reduce pesticida use. Potential donors high levels of blast resistance. Output 1 Seed of bes! gene pools distributed to FLAR and 50% of other Project progress report for Continuad demand for these populations. Enhanced Gene Poo\s. partners by the end of 1998. 1998. NARS wllllng to try out/use improved lines. Activltles Adequate funding and timely release of budget. - Introduce, identify, generate and evaluate First evaluation of the yleld potential of 3 F2BC2 populations Project progress report for Continuad support from CIRAD-CA. conducted by 1998 in 3 sltes. Number of field trials planted. 1998. gerrnplasm from different sources. Multiply seed lo Number of crosses made, OH obtained, hybrid plants recovered by Useful tralts in wild gerrnplasm can be FLARI other partners. Use AC/ embryo rescue (CM, Fleld vlslts to testing sites. MCh, ZL, CB). embryo rescue and traits identified. incorporated in improved populations. QTls identilied. Budget. NARS willíng and capable to try out/use new - ldentify and select for useful traits with the ald of molecular markers (linked to Project SB-2) (JT, FC, improved populations. CM). Output 2 Main 5 agronomic/physiological traits measured beginning Project progress report for Continuad demand by NARS for these Physiological basis for rice traits understood 1997. 1998. populations and knowledge. Two publications. Activities ! - Characterize new plant. lntrogress new plant N-management for new plant type worked out by the end of Project progress report for Continuad JIRCAS interest and support. type into LAC's gene pools ICB, CMI 1998. 1998. Adequate funding and timely released of - Understand the physiological mechanisms for First BC to new plant type made by end 1998. budget. Rice support staff in plant tolerance to low P and acid soils (CBI physiology in place. - Weed control enhanced by the use of new Mechanism for tolerance to low P/acid soils proposed. Post-Doc in place. genotypes and practicas Weed competitiva varieties developes. Germplasm adoption is higher and more - ldentification of rice cultivars with tolerance to consistent than of agronomic changas submergence '------ ---- . -- - - --- Output 3 lsolates characterized for their virulence and genetic structure. Project progress reports. Collection of blast infectad samples gives Rice pest and genetics of resistance characterized. viable isolates. Molecular markers available from BRU. Activities · Monitoring genetic and virulence diversity of lsolates. Assigned budget. Rice crosses and populations developed by pathogen. Testing breeding methods for durable rice breeders. blast resistance (CM, FCl Biotechnology unit continuas identifying · Dissecting blast resistance genes in highly molecular markers associated with resistant cultivars and make new crosses (CM, resistance. FC, linked to project SB-2) Activities · Evaluation of rice germplasm inciuding t ransgenic Rice lines with diversified resistance to Tagosodes orizicolus Worl