The One Health approach emphasizes the interconnectedness of human, animal, and environmental health, recognizing that ad- dressing complex health challenges requires collaboration across disciplines and sectors. In Southeast Asia, this approach has gained momentum as countries work to develop and implement strategies that promote health security through coordinated ac- tions. One Health platforms and regulations in Southeast Asia INTRODUCTION 1. Describe the current status of One Health platforms, strategies, action plans, and regula- tions across the Southeast Asia region. 2. Identify gaps and considerations to improve the im- plementation of One Health initiatives in Southeast Asia. The findings presented here offer a comprehensive overview of regional efforts, highlighting progress and opportunities for enhancing the One Health approach to address the region’s health and environmental chal- lenges. Objectives METHODS Literature Review: Information was collected from sources such as Google, official websites of WHO, FAO, WOAH, UNEP, and national ministries in Southeast Asia. Reports like Joint External Evalua- tion, National Action Plans for Health Security, and National Bridging Workshop reports were also re- viewed. Key Informant Interviews: We conducted 5 inter- views with One Health experts. All data was ana- lyzed for alignment with international frameworks from WHO and FAO RESULTS Figure 2: Southeast Asia One Health University Network Figure 1: One health concept In Southeast Asia, each country has established a One Health coordination mechanism for national action programs. While there is no single regional framework, several initiatives, policies, and networks support the One Health approach to address health challenges holistically. Regional strategy and framework emphasize applying OH approach to address health issues 1. ASEAN strategic framework to combat Antimicrobial Re- sistance through One Health approach (2019 – 2030) 2. ASEAN Regional strategy for preventing transmission of zoon- otic diseases from wildlife Trade 2022 3. Strategic framework for action for strengthening sur- veillance, risk assessment, and field epidemiology for health security threats in the WHO South-East Asia re- 4. ASEAN Leaders' Declaration on One Health Initiative Brunei Cambodia Indonesia Lao Malaysia Myanmar Philippine Singapore Thailand Timor Vietnam Avian influenza Salmonella Rabies E. coli O157 Anthrax Leptospirosis Brucellosis Streptococcus suis Trichinellosis Nipal Japanese encephalitis Tuberculosis Bovine spongiform encephalopathy/variant Creutzfeldt-Jakob Rift Valley fever Q fever Hantavirus Filariasis and zoonotic malaria Ebola Reston Country Name of document Year Country Name of document Year Brunei Antimicrobial Resistance National Action Plan 2019 - 2023 Cambodia Multi-Sectoral Action Plan on Antimicrobial Resistance in Cambodia 2019 - 2023 2019 - 2023 Indonesia National Action Plan on Antimicrobial Resistance Indone- sia 2017- 2019 Singapore Singapore’s One Health Framework 2012 The National Action Plan on Antimicrobial Resistance control year 2020 - 2024 National Strategic Action Plan on AMR 2017 Lao National Strategic Plan on Antimicrobial Resistance in Lao PDR 2019 - 2023 Vietnam The Vietnam One Health Strategic Plan for Zoonotic Diseases 2016 - 2022 Malaysia Malaysian Action Plan on Antimicrobial Resistance (MyAP -AMR) 2017 - 2021 Master Plan for the One Health Partership Framework for Zoonoses 2021 - 2025 Malaysian Action Plan on Antimicrobial Resistance (MyAP -AMR) 2022 - 2026 National Action Plan for Combating Drug Resistance 2013 - 2020 Malaysia Strategy for Emerging Diseases and Public Health Emergencies (MySED) II Workkplan 2017 - 2021 National Strategy for Prevention and Control of Drug Resistance in Vietnam for the period of 2023 - 2030 with a vision towards 2045 2023 - 2030 Myanmar National One Health strategic framework and action plan of Myanmar 2016- 2019 Philippines Philippines' National Strategic Plan on Zoonoses 2023 - 2028 National One Health Stategic Framework and Action Plan of Myanmar 2019 - 2023 The Philippine Action Plan to Combat Antimicrobial Resistance - One Health approach 2015 - 2020 Myanmar National Action Plan for Containment of Antimi- crobial Resistance 2017 - 2022 The Philippine Action Plan to Combat Antimicrobial Resistance, One Health approach 2019 - 2023 Thailand Thailand's National Strategic Plan for Emerging Infectious Disease - Preparedness, Prevention and Response 2013 - 2016 Timor Leste Action Plan for Timor-Leste One Health Strategic Framework 2018 - 2022 Thailand National Strategic Plan for Emerging Infectious Diseases 2017 - 2021 National Action Plan on Antimicrobial Resistance: Timor-Leste 2017 - 2020 Thailand's National Strategic Plan on AMR 2017 - 2021 One Health operation Mechanism in SEA: Almost coun- tries has form of national One Health coordination mecha- nism, which take different forms but cover similar areas (AMR, Zoonotic diseases, food safety). In some countries, OH is coordinated by an extant OH committee or working group, whereas other nations have a OH plan that a man- agement group may not permanently support. Table 1: Priority zoonotic diseases of countries in SEA which promote apply OH approach in investigating and controlling Table 2: National Action Plan/ Strategic framework applying OH approach in Southeast Asia by countries One Health network in SEA: 1.The Southeast Asia One Health University Network (SEAOHUN) focuses on strengthening the One Health workforce through education, research, and training. 2. The One Health Network Southeast Asia, funded by the EU, fosters collaboration among research projects to im- prove surveillance and response to emerging health threats. 3. Each country in Southeast Asia has also developed its own national One Health networks, involving govern- ments, academic institutions, and NGOs to advance One Health initiatives  Limited financial resources to support sustainable im- plementation.  Unclear or fragmented coordination structures across sectors and agencies.  Dependence on external funding for program activi- ties and continuity. Some challenges CONCLUSIONS To improve OH implementation, SEA must enhance multi- sector collaboration, harmonize regulations, invest in ca- pacity building, and strengthen surveillance and data shar- ing. Addressing these gaps will improve the region's ability to manage health threats at the human-animal- environment interface. Acknowledgements This review was supported by ASEAN-CGIAR Innovate for Food Regional Program and Protecting Human Health through a OH Approach (OH Initiative), ILRI. Thao Thi Bich Nguyen1* Sinh Dang-Xuan2, Steven Lam2, Hung Nguyen-Viet2, Fred Unger2 1Hanoi University of Public health, Hanoi, Vietnam; 2International Livestock Research Institute, Hanoi, Vietnam