Cover photos by: Marc Chatel and Juan Izquierdo Fertile flower and anther (left) Male-sterile flower and anther (right) Upland Line Release in Bolivia JASAYE (IRAT 170) Population Characterization DANAC, Venezuela Symposium Advanced Rice Breeding Sept. 20-24, Goionia-Brazil Breeders from 13 Countries Argentina, Bolivia, Brazil, Chile, Colombia, Cuba, El Salvador, Ecuador, France, Panama, Spain, Uruguay and Venezuela Line Development from Population PQUI-1 INIA Quilamapu, Chile Upfand Line Release in China Yunnan Province YUNLU 29 (Chinese line 1 IRAT 216) CIRAD The Centre de coopération intemationale en recherche agronomique pour le développement (CIRAD) is a Frencb research organization that specializes in agriculture in the tropics and subtropics. It is a state-owned body and was established in 1984, following the consolidation of French agricultura!, veterinary, forestry, and food technology research organizations for the tropics and subtropics. CIRAD's mission is to contribute to the economic development of these regions through research, experiments, training, and dissemination of scientific and technical information. The Center employs 1800 persons, including 900 senior staff, who work in about 50 countries. Its budget amounts to approximately l billion French francs (152 million euros), more than half of which is derived from public funds. CIRAD is made up of seven departments: CIRAD-CA (annual crops), CIRAD-CP (tree crops), CIRAD-FLHOR (fruit and horticultura! crops), CIRAD-EMVT (livestock production and veterinary medicine), CIRAD-Foret (forestry), CIRAD- TERA (territories, environment and people) and CIRAD-AMIS (advanced methods for innovation in science). CIRAD operates through its own research centers, national agricultura! research systems, or development projects. CIAT The lntemational Center for Tropical Agriculture (CIA T, its Spanish acronym) is dedicated to the alleviation of hunger and poverty in developing countries. CIA T applies science to agriculture to increase food production while sustaining the natural resource base. CIAT is one of 16 intemational agricultura! research cemers sponsored by the Consultative Group on Imemational Agricultura! Research (CGIAR). The Center' s core budget is financed by 25 donor countries, intemational and regional development organizations, and private foundations. ln 1997, the donor countries include Australia. Belgium, Brazil, Canada, Colombia, Denmark, France, Germany, Japan, the Netherlands, Norway, Spain, Sweden, Switzerland, the United Kingdom. and the United States of America. Donor organizations include the European Union (EU), the Ford Foundation. the Inter-American Development Bartk, the Intemational Development Research Center (IDRC), the lntemational Fund for Agricultural Development (IFAD), the Nippon Foundation, the Rockefeller Foundation, the United Nations Development Program (UNDP). and the World Bank. lnformation and conclusions reported in this document do not necessarily retlect the position of any donor agency. FLAR The Fund for Latín American and Caribbean lrrigated Rice (FLAR) is a rneans by which the public and private sectors of Latin American and Caribbean (LAC) countries can control and take responsibility for irrigated rice activities. FLAR began in January 1995, after an Act of Acceptance was signed by delegates from Brazil, Colombia. Venezuela. the Intemational Center for Tropical Agriculture (CIA T), and the lntemational Rice Research lnstitute (IRRI). ln 1996, Costa Rica, Panama, and the Centre de coopération intemationale en recherche agronomique pour le développement (CIRAD) became memb~rs. FLAR's mission is to promote sustainable development of irrigated rice production in LAC, that is, to make it competitive, profitable, and efficient while lowering relative prices of rice for the consumer. FLAR' s objectives are: • To provide up-to-date inforrnation on market needs and opportunities of member countries through a permanent forum. • To pursue a broad approach in regional rice activities that are of interest to all members. • lncrease sustainable rice production, that is, ensure the efficiency of production, equitable distribution of benefits, and resource conservation. • To focus mainly on irrigated rice. u 3 ocr 2002. RICE PROJECT IP-4 Improved Rice Germplasm for Latin America and the Caribbean COLLABORATIVE PROJECT BETWEEN CIRAD, CIAT, AND FLAR Rice Improvement, Using Gene Pools and Populations with Male-Sterile Gene And Conventional Breeding More Chotel , Yolima Ospina, and Jaime Borrero 1999 Report: Summary CONVENTIONAL BREEDING FOR UPLAND SAVANNA l . INTRODUCTION As it has been stated in previous reports, we ore gradually phasing out most of the activities invo lving the development of fixed lines for direct release t o NARS. Nevertheless , as we hove advanced segregating lines in the pipeline, we continue their evaluation in Colombia and with our portners. Furthermore we also evaluate ond select inter-specific progenies between Oryza sativa and O. glaberrima from WARDA and Oryza sativa lines from Madagascar. 2. LINE SELECTION in COLOMBIA In Colombia, we continue the evaluation and selection of lines for the development of fixed material to be tested by CORPOICA in t he • Altillanura" condition. • CIAT/CIRAD conventional breeding lines In 1999, 156 lines (26 families of 6 lines) were evaluated ond 16 (10.27o) were selected at LES. In each selected line 6 individual plants were harvested. • WARDA inter-specific lines and O. glaberrima accessions In 1999, one WARDA line and 3 O. glaberrimaaccessions were selected. • Lines from Madagascar Dr. Michel Vales introduced in Colombia, progenies from cr osses made in Madagascar for the highlands. F3 progenies were evaluated. No selection was made. 3. LINE RELEA SE in BRAZIL The porticipation of CIAT /CIRAD material in the different trials cont inues to be very important. The main characteristics the Brazilian praised from CIRAD/CIAT material are earlyness, plant and grain t ype. In 1998, three new CIAT lines were identified as very promising candidates for official re!ease. In 1999, 2 lines from CIAT; CT 11614-1-4-1-M (CNA8172) and CT 11251-7-2-M-M (CNA8305). were released as BONAN(:A and CARISMA respectively. 4 . LINE RELEASE in BOLIVIA In 1999, the CIRAD line IRAT 170 was released as JASAYE. It is well adapted to the r ice cropping system used by small holders. 5. USE of CIAT and CIRAO UNES in CHINA CIRAD- CA has e lose links with FCRI/Y AAS in the Yunnan Province, and had shipped many upland lines in the recent years. After screening, they were used for direct release oras parents. In 1999, FCRI/Y AAS released the variety YUNLU 29, coming from a cross between a Chinese line and IRAT 216. Lines from the CIAT / CIRAD project are under evaluation in different trials and s ites. Progenies from CIA T / CIRAD and Chinese parents are also eva luat ed. POPULATION 8REEDING FOR SAVANNA RICE 1. INTRODUCTION The upland r ice population breeding project, using recurrent selection, aims at adapting , developing and selecting tropical japonica gene pools and populations. The major characteristics we bred, for savanna condit ions are: • Tolerance of soil acidity • Resistance to diseases; rice blast (Pyricularia grisea Sacc.) • Resistance to pests, mainly r ice plant hopper ( Tagosodes oriz ico/us) • Good grain quality (translucent, long-slender grain) • Early maturity (total cycle about 115 days) 2 . POPULATION 8REEDING The activit ies we report here were conducted during the cropping season 1999 (1999 A}, from April to September at Villavicencio- Meta, · La Libertad Experiment Station" (LES). 2. 1. LINE DEVELOPMENT from RECURRENT POPULATIONS During the enhancement of gene pools and populations through recurrent selection, fert ile plants are selected. Th is selection is the starting po int for the development of promising f ixed lines for variety release and/ or potential parents for our regional partners (Argentina, Brazil, Bolivia, Venezuela and the Caribbean through CRID Net). 2 . 1 . 1 . Generation SO The f irst step in the development of f ixed lines is the selection of individual fertile plants. • Population PCf -11\0\0\2 (Second cycle of recombination \2\} Cropping season 1999 A Nineteen (19) individual ferti le plants were selected. During 1999 8, the 51 generation will be grown off-season at PES. • Population PCf -4\SA \3\1 (Third recombination cycle \3\ after one selection for acid soil \SA \) Cropping season 1999 A S ixty-one (61) individual fert ile plant s were selected. During 1999 8, the 5 1 generation will be grown off-season at PES. 2 .1. 2 . Generation 52 The generation 52 comes from fertile SO plants selected at LES during the cropping season 1998 A. The generation 51 was advanced off-season during 1998 B at PES. • Populations PCT -5\PHB\1 \O,PHB\1 ,PHB\1 PCT -A \PHB\1\0,PHB\1 ,PHB\; and PCT -4\PHB\1 \1 ,PHB\1 ,PHB\1 (Third cycle of recurrent selection for leaf blast P and Hoja Blanca HB) Cropping season 1999 A The 107 lines 52 were evaluated and 3 selected (3%) in two populations (no selection was made in PCT-5\PHB\1 \O,PHB\1,PHB\1). During 1999 B, the 53 generation 53 will be advanced off-season at PES. • Population PCT -4\SA \2\1 (Second recombination cycle \2\ after one selection for acid soil \SA \) Cropping season 1999 A The 73 lines 52 were evaluated and 5 were selected (6.8i'o) at LES. In each selected line a different number of individual plants was harvested. During 1999 B, the 23 lines 53 (3 families of 6 lines, 1 family of 2 lines and 1 family of 3 lines) will be grown off-season at PES. • Population PCT -11 \0\0\1 (First cycle of recombinat ion \1 of the original population) Cropping season 1999 A The 84 lines 52 were evaluated and 4 selected (4.8%) at LES. In each selected linea different number of individual plants was harvested. During 1999 B, the 22 lines 53 (3 families of 6 lines, 1 family of 4 lines) will be grown off- season at PES. 2 .1 . 3. Generation 54 The generation 54 comes from SO fertile plants selected during 1997 A at LES. Generations 51 and 53 were advanced off-season at PES during 1997 B and 1998 B respectively. Generations 52 and 54 were observed and selected at LES during the cropping seasons 1998 A and 1999 A respectively • Populations PCT -5\PHB\1 \O,PHB\1; PCT -A \PHB\1 \O,PHB\1 and PCT -4\PHB\1 \1 ,PHB\1 (Second recurrent selection cycle for leaf blast P and Hoja Blanca HB) Cropping season 1999 A The 35 lines 54 were evaluated at LES and 7 selected (20%) in the population PCT- 4\PHB\1\1,PHB\1. In each seleoted line 6 fertile plants were harvested. During 1999 B, the 42 lines 55 (7 families of 6 lines) will be grown off-season at PES. • Population PCT-4\5A\1\1 (First cycle of recombination \1\ after one selection for a cid soi 1 \SA \) Cropping season 1999 A The 390 lines 54 were evaluated and 44 (ll'Yo) selected at LES. In each line 6 fertile plants were harvested. During 1999 8, the 264 lines 55 (44 families of 6 lines) will be grown off-season at PES. 2 . 1. 4 . Generation 56 The generation 56 comes from SO fertile plants selected during 1996 A at LES. Generations 51, 53 and 55 were advanced off-season at PES during 1996 B, 1997 B and 1998 B respectively. Generations 52, 54 and 56 were observed and selected at LES during the cropping seasons 1997 A, 1998 A and 1999 A respectively. • Populations PCT -5\PH8\1 \O;PCT -A \PH8\1 \0 and PCT -4\PH8\1 \1 (First cycle of recurrent selection for leaf blast P and Hoja Blanca H8) Cropping season 1999 A The 150 lines 56 were evaluated and 13 (8.7'Yo) selected at LES. In each selected line, 6 individual plants were harvested. During 1999 8, the 78 lines (13 families of 6 lines) will be grown off-season at PES. 2 .1 . 5 Generation 58 The generation 58 comes from fertile SO plants selected at LES during the cropping season 1995 A. Generations 51, 53 , 55 and 57 were advanced off-season at PES during 1995 B, 1996 B, 1997 B and 1998 B respectively. Generations 52, 54, 56 and 58 were observed and selected at LES during the cropping seasons 1996 A, 1997 A, 1998 A and 1999 A respectively. • Populations PCT -5\0\0\0, PCT -A \0\0\0, and PCT -4\0\0\1 (Basic populations with no selection) Cropping season 1999 A The 301 lines 58 harvested in PES were evaluated and 44 selected (14.6'Yo) at LES. In each selected line, 6 individual plants were harvested. During 1999 8, the 264 lines (44 families of 6 lines) will be grown off-season at PES. • Population PCT -4\0\0\1 >52 Cropping season 1999 A The 72 lines 58 selected (12 famil ies of 6 lines) were evaluated, and 6 were selected (8%) at LES. In each selected fine, 6 individual plants were harvested. During 1999 B, the 36 lines (6 families of 6 lines) will be grown off-season at PES. • Populations PCT -5\0\0\0>53, PCT -A \0\0\0>53 and PCT -4\0\0\1 >53 (Basic populat ions. Plant selection in 53 Lines at PES, 1996 B) Cropping season 1999 A The 12 lines were evaluated and 1 was selected (8.3%) at LES. In each selected line, 6 individual plants were harvested. [)uring 1999 B, the 6 lines (1 family of 6 lines) will be grown off-season at PES. 2.1 . 6. Advonced generotions These generations are promising fixed lines that were selected from the f irst introduced populations from Brazil in 1992. • Populotions CNA-IRAT 5 ond CNA-IRAT A Cropping Seosons 1998 A ond 1999 A The best lines were used to set-up the regional INGER-LAC acid so il nursery called · v ivero Internacional de Observacion para América Latina" (VIOAL suelos ácidos. 2.1.7. Uplond line Registration CIAT does not register lines: when a specific line does well in a given country, the national institution of that country may decide to name and release it for commercial cult ivation. CIRAD has a mechanism by which breeders can register specif ic material. The line is named CIRAD (and is also given its local synonym, if it is the result of col laborative wor k). During 1998, we apply for their registrat ion in the CIRAD r ice catalog. In 1999, these lines were seed increosed at PES for further distr ibution to regional NARS. 2.1.8. Line dispatch to NARS [)uring 1999, we dispatched seed samples (58 generat ion) to our main collaborators in LAC and abroad, for evaluation and selection. Samples of 385 lines were shipped to: Argentina Brazil Bolivia Venezuela The Caribbean Cuba Chino Universidad de Tucumán EMBRAPA Arroz e Feijoo CIAT- Bolivia, Santa Cruz de la Sierra FONAIAP CRID Net IIA FCRI/Y AAS - Yunnan CATAS - Hainan The r esult s of the evaluation and loca l select ion will be presented next year . 2.2 . POPULATION MAINTENANCE through RECOMBINATION During 1999, no population maint enance was made, enough seed 1s stor ed in t he CIATs cold chamber. 2.3. POPULATION ENHANCEMENT The CIAT rice project emphasizes pre-breeding activities. Then we concentrate on the enhancement of populations to be distributed to NARS. The strategy is to develop and enhance gene pools and populations for well-targeted traits. They are therefore used as reservoir of promising lines and/or potential parents to be developed by national breeding programs. In the f irst 3 years of the project. we concentrated on introducing, characterizing and selecting germplasm from Brazil (former joint project between EMBRAPA Rice and Beans). From 1995 onward, we focus on enhancing the selected populations and developing new ones. 2 . 3 . 1 . Recurrent Selection Based on 52 Line Evaluation • Population PCT -4\0\0\1 Cropping season 1999 A Recombination The population PCT-4 \SA \2\1 with one cycle of recurrence and recombined twice was grown at LES to obtain the third recombination identified as PCT-4 \SA \3\1 Multi-site evaluation of 52's lines A set of 155 lines 52 from the f irst cycle of recurrence was remitted to Brazil (EMBRAPA Arroz e Feijao), Bolivia (CIAT Santa Cruz). and Venezuela (UNELLEZ) for evaluation and selection for line development. In Bolivia, 22 lines were selected and their progenies will be evaluated next year. Selection of So fertile plants Individual select ion of ferti le plant was made in the population PCT -4 \SA \2\1 (see generation SO) 2 .3 . 2. Mass Recurrent Selection for Both Sexes for "Hoja Blanca", Blast, and Major Agronomic Traits • Populations PCT -4\0\0\1, PCT -A \0\0\0, and PCT -5\0\0\0 In 1999, after completed 4 cycles of recurrence, the enhanced populations were stored in the CIATs cold chamber at PES. They would be dispatched to LAC NARS as reservoir of genotypes for line development. Results of the enhancement • Total resistance to leaf blast From the f irst cycle of selection on, a drastic reduction in the number of infected plants occurs. The use of mass recurrent selection on both sexes was efficient for the enhancement of the 3 populations. At the same t ime we selected for total leaf blast resistance, we a lso s elected for good agronomic characters. The enhanced populat ions wi ll be used as reservoir for the development of fixed lines. • Resistance to the "Hoja Blanca" virus 52 evaluati on of the enhanced populat ions showed that 97.2'Yo hove resistant and intermediate reaction to Hoja Blanca. They present a lower incidence of the disease than the lines from FEDEARROZ and ICA. The enhanced populations can be considered as good reservoirs for the development of resistant fixed lines by LAC NARS. 2. 3. 3 . Recombination of the population PCT -11 The new population was developed during 1997 and 1998. Cropping season 1999 The second cycle of recombination was completed at LES. Fertile plants of the second recombination (population PCT-11 \0\0\2) were selected, (see generation SO). 2. 4. REGISTERING NEW POPULA TIONS In 1999, one (1) new germplasm was registered, on request from our partner in China. • PYN-1 Japonica population for upland-hillsides ecosystem (FCRI/YAAS, Yunnan Province - China). 2. 5. SPECIAL STVDY. Genetic Progress , Population PCT -4 Principal objective Master Degree Thesis of Yolima Ospina at the "Universidad Nacional de Palmirau. Specific objective Evaluation of genetic progress for acid soi 1 tolerance and different agronomic characteristics like; flowering time , plant height and grain yield, through recombinat ion cycles Genetic progress after one selection cycle and effect of different recombination cycles. Method Evaluation of S1 lines in two contrasted plots for soil acidity. The statistical design was the Augmented Federer blocks composed by S1 from the four population and 6 checks (3 susceptible - CICA 8, CICA 9 y Oryzica Llanos 5 - and 3 tolerant - Oryzica Sabana 6, Oryzica Sabana 10 y CIRAD 409- to soil acidity). Data were collected and are currently processed. POPULATION BREEDING for LOWLAND RICE 1 . INTRODUCTION The population breeding project started by introducing to Colombia, different gene pools and populations previously developed in Brazil by EMBRAPA Rice and Beans Center and CIRAD, and by CIRAD in French Guyana. The germplasm was characterized at CIAT, Palmira, and the best adapted ones were used to develop new populations. Th is resulted in three popu lations that were registered in the recurrent selection catalogas PCT-6, PCT-7, and PCT-8. This work was conducted at CIAT in close collaboration with Drs. C. Martínez and E. P. Guimaraes. A gene pool was also built up , using a different gene of male sterility. The gene pool was registered as GPCT-9. A second gene pool , developed by CIRAD for temperate cl imates, was registered as GPIRAT-10. From late 1996, this basic germplasm was dispatched t o our regional partners and outside Latin America. It is the starting point for the development of population breeding in different countries. In 1999, the II International Workshop on Rice Recurrent Select ion, held in Goionia- Brazil , was the occasion for our partners to present updated informat ion on the use of population breeding. Written documents were made ava ilable to oll participants. A book is being edited by Dr. Elcio .P. Guimaraes and will be published by CIAT, CIRAD and EMBRAPA Rice and Beans. In this report. we will onlv describe the activities developed in Colombia. by the CIAT/CIRAD project. 2. POPULATION BREEDING for VENEZUELA Two populations, PCT-6 and PCT-7, were selected as the best introduced material to be used as sources of male-sterile background to develop two new local populat ions , ident if ied as PFD-1 and PFD-2. Cropping Season 1999 At CIAT Palmira the built-up of the population PFD-2 was completed and the basic population sent to DANAC-Venezuela. 3 . POPULATION BREEDING for ARGENTINA Development of local populations Argentina is developing 3 local populations: PARG-1 , PARG-2 and PARG- 3 Cropping season 1999 Population PARG-3 This new population was developed at CIAT by introduction of 50io of variability from 6 new lines into the population PCT-8. 4 . POPULA TION BREEDING for CHILE The Chilean population PQUI-1 was selected in two s ites with climatic differences (Chillan and Colchagua). The two populations, identif ied as PQUI-1\Ch\0\1 and PQUI-1\Co\1\0 were sent to CIAT Palmira for completing the second cycle of recombination. Cropping season 1999 At CIA T Palmira, we used the remnant seeds of the second recombination to perform the third cycle during the first semester of 1999. 5 . POPULATION BREEDING for URUGUAY Three (3) populations are currently developed in collaboration with CIAT: PURG-1, PURG-2 and PURG-3 Cropping Season 1999 The bui ld-up of the populations for Uruguay is under way at CIA T Palmira 6 . MAINT AINING GERMPLASM Because we manage the catalogue for r ice germplasm for recurrent selection, we also hove the r esponsibility to ensure the presence of sufficient seed in the germplasm bank. Because of sufficient disposability of seed, no multiplication was done this year. 7 . DISTRIBUTING GERMPLASM In 1999, we shipped different populations to new partners in Europe (Spain and France). 8 . REGISTERING NEW POPULA TIONS In 1999, four (4) new germplasms were registered on request from our collaborators from Chile, China and Venezuela. • PQUI-1 Japonica population for irrigated temperate ecosystem (I NIA-Quilamapu, Chile). • GPYN-2 Japonica gene poo l f or t emperate ecosyst em (FCRI/YAAS, Yunnan Province- China). • PFD-1 and PFD-2 Indica populations for tropical lowland ecosystem (DANAC- Venezuela). 9 . FIXED LINES from CHILEAN POPULATIONS through ANTHER CULTURE To fasten the development of fixed lines for Chile, the Chilean populations were processed by anther culture at CIA T. Cropping season 1999 The populations PQUI-1\Ch\2\0 and PQUI-1\Co\2\0 were grown at CIAT Palmira and plants processed at the CIA T laboratory. 325 R1 lines were produced in 1999. The R2 generation will be advanced in Colombia during the first semester of year 2000, and then dispatched to Chile. 10. FIXED LINES from CROSSES for ROMANIA through ANTHER CULTURE Two crosses (OL TENITSA 1 RUBINO, and CRISTAL 1 L 203) for cold tolerance, grain quality, and yield potential were processed by the CIA T anther culture laboratory. Cropping season 1999 A total of 61 R1 DH lines was produced. The R2 seeds were shipped to Romanía and also to France and Chile. 11 . FIXED LINES from CROSSES for SPAIN through ANTHER CULTURE On request from Spain and CIRAD-CA, four crosses were made at CIAT Palmira and wi 11 be processed by anther culture. The Fl hybrid seed was obtained, and the F1's generations will be grown during the last trimester of 1999. In early year 2000, plants will be processed by anther culture. PROYECTO ARROZ IP-4 Mejoramiento del Arroz para América Latina y e l Caribe PROYECTO COLABORATIVO ENTRE EL CIRAD, CIAT, y FLAR Mejoramiento Poblacional con Androesterilidad y Mejoramiento Convencional More Chotel, Yolima Ospina, y Jaime Borrero Informe anual 1999: Resumen MEJORAMIENTO CONVENCIONAL DEL ARROZ DE SABANAS 1. INTRODUCCIÓN Como ya se ha planteado en los informes de los precedentes años, estamos paulatinamente terminando las actividades de desarrollo de líneas f ijas para su entrega inmediata a los programas nacionales. Sin embargo, existen líneas avanzadas creadas por el proyecto cuya evaluación sigue, tanto en Colombia como en otros países. A demás estamos evaluando y seleccionando líneas procedentes de cruces inter- especificos entre Oryza sativay O. glaberrima introducidas de WARDA , como también líneas de Oryza sativa introducidas de Madagascar . 2 . SELECCIÓN de LÍNEAS en COLOMBIA En Colombia, continuamos la evaluación y selección de líneas para el desarrollo de líneas fijas que serán entregadas a CORPOICA para su adaptación a las condic iones de la Alt illanura Colombiana. • Líneas del proyecto convencional CIAT /CIRAD En 1999, 156 líneas (26 familias de 6 líneas) fueron evaluadas y 16 (10.2 'Yo) seleccionadas. En cada una se escogieron 6 plantas individuales. • Líneas inter-especificas de W ARDA y de O. glaberrima En 1999, una línea de WARDA y 3 de O glaberrima fueran seleccionadas. • Líneas de Madagascar Descendencias de cruces hechos en Madagascar para secano de laderas fueron introducidas en Colombia , por el Dr. Michel Vales. Descendencias F3 fueran evaluadas. Ninguna líneas fue seleccionada. 3 . LANZAMIENTO de LÍNEAS en BRASIL La participación del material vegetal del proyecto CIA T / CIRAD siga siendo muy importante. Las principales características de este material son precocidad, el tipo de grano y t ipo de planta bien adaptado a las condiciones brasileñas. En 1998, 3 nuevas líneas CIAT fueron identificadas como promisorias para su lanzamiento. En 1999, 2 de ellas; CT 11614-1-4-1-M (CNA8172) y CT 11251-7-2-M-M (CNA8305), fueron lanzadas como BONAN~A y CARISMA respectivamente. 4. LANZAMIENTO de LÍNEAS en BOUVIA En 1999, la línea IRAT 170 del CIRAD fue lanzada como JASAYE. Ella es muy bien adaptada a las condiciones de cultivo de los pequeños agricultores. 5. UTILIZACIÓN de las LÍNEAS del CIAT y del CIRAD en CHINA El CIRAD-CA tiene estrecha colaboración con el FCRI/YAAS en la Provincia del Yunnan. Se enviaron líneas de arroz de sec~no que, después su evaluación y selección fueron lanzadas como cultivar o utilizadas como progenitores potenciales (IRAT 104, IRAT 359 y IRAT 216 respectivamente). En 1999, el FCRI/YAAS lanzo la variedad YUNLU 29, que proviene de un cruce entre una línea china y la IRAT 216. Líneas CT como también descendencias de cruces entre líneas chinas y CT están s iendo evaluadas en diferentes ensayos. MEJORAMIENTO POBLACIONAL PARA ARROZ DE SABANAS 1. INTRODUCCIÓN El proyecto de mejoramiento poblacional ut ilizando la selección recurrente tiene como objetivo la adaptación, el desarrollo y la selección de poblaciones y acervos genéticos de tipo Japonico tropical. Las principales características que se buscan mejorar para las condiciones de sabanas son las siguientes: • Tolerancia a la acidez del suelo • Resistencia a Piricularia (Pyricularia grisea Sacc.) • Resistencias al insecto vector del virus de la Hoja Blanca ( Tagosodes orizico/us) • Buena calidad de grano (translucido y largo- fino) • Precocidad (ciclo total de 115 días) 2. MEJORAMIENTO POBLACIONAL Las actividades reportadas en este informe fueron conducidas durante la estación de cultivo, Abril - Septiembre de 1999 en Villavicencio - Meta en la , Estación Experimental "La Libertad" (EELL). 2 . l . DESARROLLO DE LÍNEAS de POBLACIONES RECURRENTES Durante el mejoramiento de las pob laciones por selección recurrente, se seleccionan plantas fért iles. Estas son el punto de partida para el desarr ollo de líneas f ijas o de progenitores potenciales, en colaboración con nuestros principales socios (Argentina , Brasil, Bolivia, Venezuela y el Caribe, CRID Net). 2. 1 . 1 . Generación SO El primer paso del desarrollo es la selección de plantas fértiles . • Población PCT -11\0\0\2 (Segundo ciclo de recombinación \2\) 1999 A 19 plantas individuales fueron seleccionadas. 1999 B, la generación 51 se sembrara en EEP. 1999 A • Población PCT -4\SA \3\1 (Tercer ciclo de recombinación\3\ después de una selección para suelos ácidos \SA \ ) 61 plantas individua les fueron seleccionadas. Durante 1999 B, la generación 51 se sembrara en EEP. 2.1 . 2. Generación S2 La generación 52 viene de las plantas fértiles SO seleccionadas durante 1998 A en la EELL. Se ha avanzado la generación 51 en EEP durante 1998 B. • Poblaciones PCT -5\PHB\1 \O,PHB\1,PHB\1 PCT -A \PHB\1 \O,PHB\1,PHB\1 ; y PCT -4\PHB\1 \1,PHB\1 ,PHB\1 (Tres ciclos de selección recurrente para Piricularia en hojas P y Hoja Blanca HB) 1999 A 107 líneas 52 fueron avaluadas y se seleccionaron 3 (3'Yo) en dos poblaciones (ninguna selección se hizo en la población PCT-5\PHB\l\O,PHB\1,PHB\1). 1999 8, !a generación 53 se sembró en la EEP. • Población PCT -4 \SA \2\1 (Segundo ciclo de recombinación \2\ después de una selección para suelos ácidos \SA \) 1999 A 73 líneas 52 fueron evaluadas y se seleccionaron 5 (6.8'Yo) en EELL. En cada línea seleccionada se escogieron números distintos de plantas. 1999 B. las 23 líneas 53 (3 famil ias de 6 líneas, 1 familia de 2 líneas y 1 familia de 3 líneas) se sembró en la EEP. • Población PCT -11\0\0\1 (Primer ciclo de recombinación\1 de la población original) 1999 A 84 líneas 52 fueron evaluadas y se seleccionaron 4 (4.8'Yo) en EELL. En cada línea seleccionada se escogieron números distintos de plantas. 1999 B. las 22 líneas 53 (3 fami lias de 6 líneas, 1 familia de 4 líneas) se sembraron en EEP. 2 . 1 . 3. Generación S4 La generación 54 viene de plantas fértiles SO seleccionadas durante 1997 A en la EELL. Las generaciones 51 y 53 se avanzaron en la EEP, durante 1997 B y 1998 B respectivamente. Las generaciones 52 y 54 se observaron y se seleccionaron en la EELL durante 1998 A y 1999 A respectivamente. • Poblaciones PCT -5\PHB\1 \O,PHB\1 ; PCT -A \PHB\1 \O,PHB\1 y PCT -4\PHB\1 \1,PHB\1 (Segundo ciclo de selección recurrente para Piricularia P y Hoja Blanca HB) 1999 A Las 35 líneas 54 se evaluar on en la EELL y 7 se seleccionaron (17'Yo) en la pob lación PCT- 4\PHB\l\1,PHB\1. En cada línea seleccionada se cosecharon 6 plantas individuales. 1999 B, las 42 líneas 55 (7 familias de 6 líneas) se sembraron en la EEP. • Población PCT -4 \SA \1 \1 (Primer ciclo de recombinación \1\ después de una selección para suelos ácidos \SA \) 1999 A Las 390 líneas 54 fueron evaluadas y se seleccionaron 44 (11io) en la EELL. En cada una se escogió 6 plantas individuales. 1999 B, las 264 líneas 55 (44 familias de 6 líneas)se sembraron en la EEP. 2.1 . 4. Generación S6 La generación 56 viene de las plantas 50 seleccionadas en la EELL, en 1996 A. Las generaciones 51, 53 y 55 se avanzaron en la EEP durante 1996 B, 1997 B y 1998 B respectivamente. Las generaciones 52, 54 y 56 se observaron y se seleccionaron en la EELL durante 1997 A, 1998 A y 1999 A respectivamente. • Poblaciones PCT -5\PHB\1 \0; PCT -A \PHB\1 \0 y PCT -4\PHB\1 \1 (Primer ciclo de selección recurrente para Piricu laria en hoja P y Hoja Blanca HB) 1999 A Las 150 líneas 56 fueron evaluadas y se seleccionaron 13 (8.6io) en la EELL. En cada una de las líneas seleccionadas se cosecharon 6 plantas individuales. 1999 B, las 78 líneas (13 familias de 6 líneas) se sembraron en la EEP. 2.1.5 Generación se La generación 58 viene de las plantas 50 seleccionadas en la EELL durante 1995 A. Las generaciones 51, 53, 55 y 57 fueron avanzadas en la EEP durante 1995 B, 1996 B. 1997 By 1998 B respectivamente. Las generaciones 54, 56 y 58 se observaron y se seleccionaron en la EELL durante 1996 A, 1997 A, 1998 A y 1999 A respectivamente. • Poblaciones PCT -5\0\0\0, PCT-A \0\0\0, y PCT -4\0\0\1 (Poblaciones base sin selección recurrente) 1999 A Las 301 líneas 58 se evaluaron y se seleccionaron 44 (14.6io) en la EELL. En cada línea seleccionada se cosecho 6 plantas individuales. 1999 B, las 264 líneas (44 familias de 6 líneas) se sembraron en la EEP. • Población PCT -4\0\0\1 >S2 1999 A Las 72 líneas 58 se evaluaron y se seleccionaron 6 (8io) en la EELL. En cada una se cosecharon 6 plantas individuales. 1999 B, las 36 líneas (6 familias de 6 líneas) se sembraron en la EEP. • Poblacion~s PCT-5\0\0\0>S3, PCT-A\0\0\0>S3 y PCT -4\0\0\1 >S3 (Población base con selección de plantas en líneas 53 en la EEP en 1996 B) 1999 A Las 12 líneas 58 se evaluaron y se selecciono 1 (8'Yo) en la EELL. En cada una se cosecharon 6 plantas individuales. 1999 B, las 6 líneas (1 familia de 6 líneas) se sembraron en la EEP. 2 .l. 6. G~n~racion~s avanzadas Estas generaciones son representadas por líneas fijas promisorias seleccionadas a partir de las poblaciones introducidas de Brasil en el año de 1992. • Poblacion~s CNA-IRAT 5 y CNA-IRAT A 1998 A y 1999 A Las mejores líneas fueron utilizadas para formar el ensayo regional del INGER-LAC · vivero Internacional de Observación para América Latina" (VIOAL suelos ácidos). 2 .1. 7 . R~gistró d~ líneas El CIAT no registra líneas, pero el CIRAD tiene un mecanismo por el cual los mejoradores pueden registrar sus líneas. Las líneas son llamadas de CIRAD y se hace referenc ia a los obtentores. Durant~ ~1 año 1998, hemos aplicado por registrar algunas líneas en el catalogo del CIRAD. En 1999. estas líneas fueron multiplicadas y distribuidas a los programas nacionales. 2.1.8. Envío d~ lín~as a los programas nacional~s Durante ~1 año d~ 1999, hemos enviado muestras de 385 líneas 58 a los siguientes socios, para su evaluación y selección. Argentina Brasil Bolivia Ven~zu~la El Caribe Cuba China Universidad de Tucumán EMBRAPA Arroz e Feijao CIAT- Bolivia, Santa Cruz de la Sierra FONAIAP CRID Net IIA FCRIIYAAS - Yunnan CATAS - Hainan Los resultados de las evaluaciones serán presentados en el informe del año próximo. 2.2. MANTENIMIENTO DE LAS POBLACIONES con RECOMBINACIÓN Durante 1999, no se ha multiplicado ningún germoplasma, pues tenemos semilla suficiente guardada en el cuarto frío de CIA T. 2 . 3 . MEJORAMIENTO POBLACIONAL EL proyecto Arroz de CIAT se enfoca en el pre-mejoramiento. Entonces nos concentramos en el mejoramiento de poblaciones para su posterior entrega a los programas nacionales. La estrategia es de desarrollar y mejorar germoplasma para características de interés. Estos germoplasmas mejorados s irven como reservorio para el desarrollo de líneas fijas y/o progenitores potenciales para los programas nacionales. 2 . 3 . l . Selección recurrente con evaluación de descendencias S2 • Población PCT -4\0\0\1 1999 A Recombinación La población PCT-4 \SA \2\1 con un ciclo de selección recurrente seguido de dos recombinaciones se sembró en la EELL para obtener el tercer ciclo de recombinación identificado como PCT-4\SA \3\1 Evaluación multi - local de líneas S2 155 líneas 52 fueron enviadas a Brasil (EMBRAPA Arroz e Feijéío), Bolivia (CIAT Santa Cruz), y Venezuela (UNELLEZ) para evaluación, selección y desarrollo de líneas. En Bolivia, 22 líneas fueron seleccionadas. Selección de plantas fértiles SO Selección de plantas fértiles se hizo en la población PCT-4\SA\2\1 (ver generación SO) 2 .3.2. Selección recurrente masal en ambos sexos para Piricularia, Hoja Blanca, y caracteres agronómicos . • Poblaciones PCT -4\0\0\1 , PCT -A \0\0\0, y PCT -5\0\0\0 Durante 1998 B, después de haber completado 4 ciclos de selección recurrente, las poblaciones mejoradas se almacenaron en el cuarto frío de CIAT. Ellas serán enviadas a los programas nacionales , para analizar su comportamiento y seleccionarlas. Resultados del mejoramiento • Resistencia total a Piricularia hoja A partir del primer ciclo de selección se observo una muy fuerte disminución del numero de plantas infectadas. • Resistencia al virus de la Hoja Blanca La evaluación de líneas 52 del germoplasma mejorado mostró que 97.2% de ellas presentaron resistencia o reacción intermed iaria al virus de la Hoja Blanca. 2 . 3. 3. Recombinación de la población PCT -11 Esta población se sintetizo durante 1997 y 1998. 1999 A Se completo el segundo ciclo de recombinación en la EELL. Plantas fértiles SO se seleccionaron en la población PCT-11\0\0\2 (ver generación SO). 2 . 4 . REGISTRA CIÓ N de NUEVAS POBLACIONES En 1999, una población se registro a pedido de la China. • PYN-1 Población Japonicapara las condiciones de ladera (FCRI/YAAS, Provincia de Yunnan - China). 2.5 . ESTUDIO ESPECIAL. Progreso genético, población PCT -4 Objetivo principal Tesis de grado de Maestría de la Ingra. Yolima Ospina. Universidad Nacional de Palmira. Objetivo especifico Evaluación del progreso genético para tolerancia a suelo ácido y otros caracteres agronómicos como, floración altura de planta rendimiento en diferentes ciclos de recombinación. Progreso genético después de un ciclo de selección y efecto de diferentes ciclos seguidos de recombinación. Método Evaluación de líneas 51 en dos ambientes contrastantes de acidez de suelo. El diseño estadístico es el de bloques aumentados de Federer compuestos de líneas 51 y 6 testigos (3 susceptibles- CICA 8, CICA 9 y Oryzica Llanos 5 -y 3 tolerantes - Oryzica Sabana 6, Oryzica Sabana 10 y CIRAD 409- a la acidez del suelo). Los datos están siendo procesados . MEJORAMIENTO POBLACIONAL para ARROZ DE RIEGO 1 . INTRODUCCIÓN El proyecto de mejoramiento poblacional comenzó con la introducción en Colombia de diferentes acervos genéticos y poblaciones desarrolladas en Brasil por el EMBRAPA Arroz e Feijao y el CIARD, y en Guyana Francesa por el CIRAD. El germoplasma introducido fue caracterizado en CIA T, Palmira , y los mejores adaptados fueron ut ili zados para crear nuevas poblaciones. El resultado fue la obtención de 3 nuevas poblaciones que se registraron como PCT-6, PCT-7, y PCT-8. Este trabajo se hizo en colaboración con los Drs. C. Martínez y E. P. Guimaraes. Se creo también un acervo genético con un nuevo gene de androesterilidad, diferente del ut ili zado anteriormente. El acervo se registro como GPCT-9. Otro acervo, anteriormente desarrollado por el CIRAD para las condiciones de clima templado fue registrado como GPIRA T -10. A partir del final del año de 1996, los germoplasmas base fueron enviados a diferentes programas nacionales en América Latina. Estos germoplasmas fueron el punto de partida de los programas de mejoramiento poblacional en los países. En septiembre de 1999, el Segundo Taller Internacional de Selección Recurrente en Arroz se real izó en Goiania-Brasil. Durante este evento, los diferentes colaboradores presentaron los avanzos alcanzados en sus proyecto de mejoramiento. Un documento con todas las publicaciones fue distribuido a los participantes. Se debe editar un libro que será publicado conjuntamente por CIAT, CIRAD y EMBRAPA Arroz e Feijao. En este informe solo estaremos presentando las actividades desarrolladas en Colombia por el proyecto CIAT/CIRAD . 2. MEJORAMIENTO POBLACIONAL para VENEZUELA Las dos poblaciones, PCT-6 y PCT-7, fueron seleccionadas como las mejores dentro de las introducidas. Ellas fueron utilizadas como fuente de androesterilidad para crear dos poblaciones locales identificadas como PFD-1 y PFD-2. 1999 La población PFD-2 fue sintetizada en CIAT Palmira y se envío a DANAC-Venezuela. 3 . MEJORAMIENTO POBLACIONAL para ARGENTINA Desarrollo de poblaciones locales En la Argentina se están creando 3 pob laciones denominadas PARG-1 , PARG-2 y PARG- 3 1999 Población PARG-3 Esta población se esta sintetizando en CIAT Palmira por la introducción de 6 líneas, representando 50io de nueva variabilidad, en la población PCT-8. 4 . MEJORAMIENTO POBLACIONAL para CHILE La población chilena PQUI-1 fue seleccionada en dos sitios (Chillan y Colchagua) que corresponden a dos situaciones climáticas diferentes. Las dos pob laciones se identifican como PQUI-1 \Ch\0\1 y PQUI-1 \Co\1 \0 se mandaran al CIAT Palmira para completar sur segundo ciclo de recombinación. 1999 Se completo el tercer ciclo de recombinación de ambas pob laciones durante e l primer semestre de 1999. 5 . MEJORAMIENTO POBLACIONAL para URUGUAY 3 poblaciones están siendo creadas en colaboración con el CIAT Palmira. Son ellas: PURG-1 , PURG-2 y PURG-3 1999 La creación de estas poblaciones se esta realizando en CIAT Palmira. 6 . MANTENIMIENTO del GERMOPLASMA El proyecto CIA T /CIRAD esta encargado de registrar e l germoplasma para selección recurrente y consecuentemente de su mantenimiento (multiplicación). Como tenemos suficiente semilla guardada en el cuarto frío de CIA T ninguna multiplicación se ha hecho este año. 7 . ENVIO de GERMOPLASMA En 1999, se envío unas poblaciones base a nuevos socios, en Europa (España y Francia). 8. REGISTRACIÓN de NUEVOAS POBLACIONES En 1999, se registraron 4 nuevas poblaciones a pedido de nuestros colaboradores en Chile, China y Venezuela. • PQUI- 1 Población Japonica para las condiciones de clima templado (INIA-Quilomapu, Chile). • GPYN-2 Acervo genético Japonica paro las condiciones de cl ima templado (FCRI/Y AAS, Yunnan Provincia - China). • PFD-1 y PFD-2 Poblaciones Indica para riego trop ical (DANAC - Venezuela). 9. DESARROLLO DE LÍNEAS FIJ' AS d~ POBLACIONES CHILENAS por CULTIVO DE ANTERAS Para adelantar la obtención de líneas fijas para Chile, las dos poblaciones chilenas pasaron por cultivo de anteras en el laboratorio de CIA T. 1999 Las poblaciones PQUI-1\Ch\2\0 y PQUI-1\Co\2\0 se sembraran en CIAT Palmira y sus plantas fueron procesadas en el laboratorio. 325 líneas Rl fueron producidas y la generación R2 será avanzada en Colombia y luego su semilla enviada a Chile. 10. DESARROLLO DE LÍNEAS FIJAS d~ CRUCES CONVENCIONALES para RUMANIA Dos cruces simples, (OL TENITSA 1 RUBINO, y CRISTAL 1 L 203) específicamente hechos para la tolerancia al frío , rendimiento y calidad de grano, fueron procesados en el laboratorio de CIA T. 1999 Un total de 6llíneas Rl fueron producida y su semilla enviada a Rumania, Francia y Chile. 11 . DESARROLLO DE LÍNEAS FIJAS d~ CRUCES CONVENCIONALES para ESPAÑA A pedido de España y del CIRAD-CA , 4 cruces simples se hicieran en CIAT, para después ser procesados por cultivo de anteras. La semil la Fl se ha producido, la generación Fl se sembró en el segundo semestre de 1999. Las plantas serán procesadas durante el primer semestre del año 2000. CHAPTER I PRESENTA TION 1. CIAT RICE PROJECT 2. FUND FOR LATINAMERICAN AND THE CARIBBEAN IRRIGATED RICE (FLAR) 3. CIRAD/CIAT/FLAR COLLABORATIVE PROJECT 3 .1 . CONVENTIONAL BREEDING 3. 2. POPULA TION BREEDING 4. ACKNOWLEDGMENTS CHAPTER I PRESENTA TION 1. CIAT RICE PROJECT CIA T is developing its research activities according to a project management system. The title of the rice project is "Improved Rice Germplasm for Latin America and the Caribbean". Its breakdown structure is presented in Appendix 1. 2 . FUND FOR LATINAMERICAN IRRIGATED RICE (FLAR) FLAR was created in 1995. In September 1996, CIRAD signed an agreement with the Fund and became member. Beginning in 1997, the CIRAD/CIAT collaborative project developed research activities with FLAR on recurrent selection breeding for irrigated lowland rice. 3. CIRAD/CIAT/FLAR COLLABORATIVE PROJECT The 1999-work plan of the CIAT / CIRAD/FLAR collaborative project is presented in Appendix 2. The 4th Strategic Alliance Meeting between CIAT, CIRAD, INRA , and IRD (former ORSTOM) was held during June 21-24, 1999 at Montpellier, France. The conclusion of the meeting fo r Rice Improvement pointed out that, quote: "Development of Improved Rice Populations using recurrent selection and conventional breeding is being accomplished very efficiently in terms of t echnical and scientific results , as well as development and training activities. CIA T considers that the work conducted by More Chotel is clearly a priority for CIAT". 3 .1 . CONVENTIONAL BREEDING In 1996, after the leaving of the CIA T scientist in charge, the activities conducted by the conventional breeding project for upland savanna rice were reduced. But were reactivated to a certain extent since 1997 because of the availability of promising lines previously developed. In 1998 and 1999, upland lines were shipped to new partners identified during 1997: • Colombia, Ministry of Agriculture (Atlantic Coast region), • Argentina, National University of Tucumán, • Paraguay, Rice Producers Association of Itapua, • Peru, National Maize and Rice Program, and • Venezuela, Fondo Nacional de Investigación Agropecuarias (FONAIAP). We maintain strong relationships with: • Brazil , EMBRAPA Rice and Beans Center, • Bolivia, CIAT Santa Cruz, and • Venezuela, National Experimental University of the Oriental Plains "Ezequiel Zamora" (UNILLEZ). In 1999, three (3) lines were released in two countries. Bolivia: JASA YE (IRAT 170), and Brazil: BONAN(:A and CARISMA In the Caribbean, Guyana, Belize and Cuba were identified as new partners, through the Caribbean Rice Industry Development Network (CRIDNet). 3. 2. PO PULA TION BREEDING Introduction Genetic uniformity, or lack of genetic diversity, is of major concern to breeders, geneticists, and the agricultura! community in general. In many crops, genetic improvement is usually accompl ished by reducing genetic diversity in the gene pools used to develop new variet ies. But genetic uniformity is now considered as increasing a crop 's potential vulnerability to disasters caused by biotic or abiotic constraints. In Latin America, the genetic diversity of rice varieties depends on a small genetic core of land roces (1 in Appendix 3). One way of broadening the genetic base of Latin American rice and assessing the genotype-by-environment interaction is to identify specific potential parents and pool them to develop new, genetically broad-based, breeding material. CIAT and CIRAD new breeding strategies focus on developing and improving populations to provide sources of fixed lines or potential parents with specific traits required by national breeding programs. One suitable breeding method to achieve this goal is recurrent selection. Main activities Started in 1992, the CIRAD/CIAT rice improvement collaborative project introduced from Brazil and French Guyana, and developed in Colombia (CIAT Palmira and Villavicencio) gene pools and populations segregating for a male-sterile recessive gene (2 in Appendix 3). At first, the main objectives of the project were: • To understand the performance of the introduced germplasm, • To maintain and multiply the germplasm by harvesting fecundated male-sterile plants, • To select fertile plants for fixed lines or potential parents, • To start population enhancement, using recurrent selection, and • To create, in collaboration with LAC NARS, new populations by incorporating local variability into the best-adapted populations. Since 1995, we are focusing with ours regional partners, on line development and enhancement of different upland and lowland populations, especially for blast resistance, earliness, tolerance to acid soils, grain quality and grain yield for upland ecosystem. For lowland conditions (tropical, sub- tropical, and t emperate climate) the objectives are resistance to rice blast and Hoja Bloca virus, tolerance to cold, and grain yield and quality. As International Centers, we also develop germplasm with broad genetic base, in order to gather, and maintain variability. To these germplasms we apply low selection pressure to maintain high variability and adaptation to broad ecoregional regions. CIAT and CIRAD breeding strategies focus on developing and improving populations, (pre-breeding) and gradually phasing out of the development of finished lines for direct release. Pre-breeding aims at providing National Programs (NARS) with enhanced genetic reservoirs for potentiallines and/or parents with specific traits. Expertise sharing The expertise of the CIA T 1 CIRA D collaborative project on recurrent selection is shared with regional NARS through activity reports, didactic documents, field visits, workshops and training. International training Courses The first International Course on Rice Recurrent Selection Breeding was held at CIAT in 1996. Fifteen scientists from 13 countries attended the course. Back in their home countries, many began using population breeding. The second one (International and National) took place in 1998, in Venezuela and was organized by the DANAC Foundation. The third one is planned to be held in Cuba in June 2001. Workshops The First International Workshop on Rice Recurrent Selection was held in Goicnia-Brazil, in March 1995. It was jointly organized by CIAT/CIRAD and EMBRAPA Rice and Beans Center. The second one was held at the same place, and by the same Institutions, during September 21-24. Scientists from 10 countries (Argentina, Bolivia, Brazil, Colombia, Chile Cuba, El Salvador, Panama, Uruguay, and Venezuela) presented their results on population breeding. The CIRAD/CIAT project presented 4 communications. Observers from Spain and the Food and Agriculture Organization of the United Nations (FAO) also attended the meeting. At the end of the workshop, FAO supported the idea of establishing a formal group on Advanced Breeding Methods (Hybrid rice, population breeding, and biotechnology) in Latin America and the Caribbean. The group named "Advanced Rice Breeding Group" (Grupo de Mejoramiento Genético Avanzado en Arroz - GRUMEGA in Spanish) - was formally set-up, anda project profile written by CIAT/CIRAD and EMBRAPA. FAO will actas a facilitator , helping presenting the project to donors. Monitoring progress We are monitoring with our LAC partners, the use of the basic and enhanced populations developed by the project. We also help doing special work at CIA T Palmira; • development of specific populations for future local use, • generation advance, recombination cycles, and • anther culture. Registering populations for recurrent selection breeding On request from breeders, the project is in charge of registering new populations developed by NARS. In 1999 , five populations were registered. Their origin and genetic composition are presented in Appendix 4. • PQUI-1 developed by INIA Quilamapu-Chile, • PFD-1 and PFD-2 developed by Fundación DANAC- Venezuela, and • PYN-1 and GPYN-2 developed by the Food Crops research Institute (FCRI) of the Yunnan Academy of Agricultura! Sciences (YAAS) Yunnan Province -China, Expertise transfer Recurrent selection germplasm crosses continents. On request, we hove shipped populations to Europe (France and Spain) and Asia (China). In Europe, we initiate collaboration with the French Center for Rice (Centre Fran~ais du Riz) anda Spanish Seed Company. In China, the project collaborates with FCRI/YAAS. FCRI has developed two local japonica germplasms based on the CIAT / CIRAD population PCT-5. The two germplasms are identif ied as PYN-1 and GPYN-2 for upland and irrigated conditions respectively. A concept note for an Inter-Center Project on expertise t ransfer to West Afr ica (CIAT/ CIRAD, WARDA and CORAF) was prepared. CIAT and CIRAD are making the follow-up. 4. ACKNOWLEDGMENTS This document reports the research activities developed during the second semester of 1998 (1998 B) at CIAT headquarters (Palmira Experimental Station (PES), Department of Valle, Colombia) and the first semester of 1999 (1999 A), and "La Libertad" Experiment Station (LES) Villavicencio, Department of Meta, Colombia). In Latín America and the Caribbean, research activities are conducted in close collaboration with scientists from different Countries. In Asia, we initiated, in 1997 and 1999, a collaboration with two Provinces of the People's Republic of China: - The Yunnan Province, with FCRI/YAAS and, - The Hainan Province, with the Chinese Academy of Tropical Agricultura! Sciences (CATAS). In Europe, collaboration will start during the next cropping season with the French Center for Rice (Centre fran~ais du Riz -CFR- in French) and a Spanish Seed Company. We would like to acknowledge the excellent collaboration received from the following scientists: Argentina María Antonia Marassi Juan Antonio Marassi Marta Genoveva Nicosia José Villegas Bolivia Brazil Roger Taboada Paniagua R. Guzmán Emilio da Maia de Castro Elcio Perpetuo Guimaraes James Taillebois Universidad de Corrientes Universidad de la Plata Universidad Nacinal de Tucumán Universidad Nacinal de Tucumán CIA T Santa Cruz de la Sierra CIA T Santa Cruz de la Sierra EMBRAPA Arroz e Feijao EMBRAPA Arroz e Feijao CIRAD-CA Chile Roberto Alvarado Santiago Ignacio Hernaiz Lagos China Tao Dayun Li Kai Mian Colombia Hernando Delgado Huertas Edgar Corredor Costa Rica Randolph Campos Morera Cuba Rene Perez Polanco El Salvador Ramón Eduardo Servillón France Guy Clément Panama Ariel E. Jaén Sánchez Spain José Pedro Castells Franch Venezuela Alberto Herrera G. Eduardo Graterol Carlos Gamboa Gelis Torrealba Uruguay Fernando Blaz Pérez de Vida INIA-Quilamapú INIA -Qui lamapú FCRI/YAAS, Yunnan Province CATAS, Hainan Province CORPOICA-Regional 8 FEDEARROZ Ministry of Agriculture IIA Sancti-Spiritus CENTA CIRAD/CFR Universidad de Panama Semillas Certificadas Castells Universidad (UNILLEZ) DANAC - Fundación Polar DANAC - Fundación Polar FONAIAP INIA-Treinta y Trés CHAPTER II CONVENTIONAL BREEDING FOR UPLAND SAVANNA 1 . INTRODUCTION 2 . LINE SELECTION in COLOMBIA 3 . LINE RELEASE in BRAZIL 4. LINE RELEASE in BOLIVIA 5. USE of CIAT and CIRAD LINES in CHINA CHAPTER II CONVENTIONAL BREEDING FOR UPLAND SAV ANNA l . INTRODUCTION As it has been stated earlier, we are gradually phasing out most of the activities involving the development of fixed lines for direct release by NARS. Nevertheless as we hove advanced segregating lines in the pipeline, we continue their evaluation, in Colombia and with our partners. Furthermore we also evaluate and select inter-specific progenies between Oryza sativa and O glaberrima from WARDA and Oryza sativa lines from Madagascar. In 1996 B, we sent savanna upland lines (F4 and F5 generation) to EMBRAPA Rice and Beans Center for observation and seed increase. These lines were sent back to CIAT, Palmira, in 1997. In 1998 B, the lines were seed increased and dispatched to different countries. 2. LINE SELECTION in COLOMBIA In Colombia, we continue the evaluation and selection of lines for the development of fixed material to be tested by CORPOICA in the "Aitillanura" condition. We also evaluate and select inter-specific progenies between Oryza sativa and O glaberrima from WARDA, and Oryza sativa lines from Madagascar. • CIAT/CIRAD conventional breeding lines During 1998 A, we selected 24 lines that presented the best characteristics of adaptation to acid soil condition. In 1999, 156 lines (26 families of 6 lines) were evaluated and 16 (10.2'Yo) were selected at LES (table 1). In each selected line 6 individual plants were harvested. • WARDA inter-specific lines and O. g/aberrima accessions Since 1996 we are evaluating this germplasm for selecting potential parents to be incorporated in the development of new populations. In 1999, one WARDA line and 3 O glaberrima accessions were selected (tables 2 and 3). • Lines from Madagascar Dr. Michel Vales introduced progenies from crosses made in Madagascar for the highlands, in Colombia. F3 progenies were evaluated (table 4). The lines present low vigor and non-adapted plant type. No selection was made. 3. LINE RELEASE in BRAZIL The participation of CIAT /CIRAD material in the different trials continues to be very important. The main characteristics the Brazil ian praised from CIRAD/CIAT material are earlyness, plant and grain type. During the period 1994-1997, 4 lines were released in different S tates of Brazil. They are: CONFIAN~A (States of Roraima and Minas Gerais), CANASTRA (States of Minas Gerais, Goias, Tocantins, Piaui and Maranhao), MARAVILHA (Goias, Mato Grosso, Tocantins , Para, Roraima and Rondonia), and PRIMAVERA (States of Goias, Tocantins, Maranhao, Piaui, Mato Grosso and Mato Grosso do Sul). From these, the CIAT conventional breeding program developed 2: CANASTRA CT 7415-6-5-1-2-B MARAVILHA CT 6516-23-10-1-2-2-B In 1998, three new CIAT lines were identified as very promising candidates for official release. CNA8172 CT 11614-1-4-1-M CNA8305 CNA8436 CT 11251-7-2-M-M CT 11251-7-2-M-1-M- M In 1999, CT 11614-1-4-1-M (CNA8172) and CT 11251-7-2-M-M (CNA8305) were released as BONAN9A and CARISMA respectively. 4 . LINE RELEASE in BOLIVIA In 1999, the CIRAD line IRAT 170 was released as JASAYE. I t is well adapted to r ice cropping system used by small holders. 5 . USE of CIAT and CIRAD LINES in CHINA CIRAD- CA has close links with FCRI/YAAS, and had shipped many upland lines in the recent years. After screening, they were used for direct release or as parents. In 1996 the CIRAD line IRAT 104 was released in the Yunnan Province. In 1998, the line IRAT 359 showed very promising results and acceptability by farmers in demonstration fields. It will be released next year. In 1999, FCRI/YAAS released the variety YUNLU 29, coming from a cross between a Chinese line and IRAT 216. In this framework, in 1995, the CIAT/CIRAD project sent to China the f irst set of savanna lines developed in Brazil and Colombia. Preliminary results were promising. In 1997, line CT 9278-11-14-2-1-M was identified as very promising parent. New CT lines are under evaluation in different trials and sites as well as progenies from CIA T and Chinese parents. Tablel. Evaluation of lines from the conventional bruding projut Le libertad experimental station, 1999A Nbr. F1ekl Nbr. Ottgln ,_ Vg 81 Bl F1 LSc BS GD N81 1 2 3 • 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 36 39 40 41 ~ 43 44 45 48 47 48 49 1MM 5990001 5990002 5990003 599()()0.4 5990005 T1 T2 T3 5990006 5990007 5990006 5990009 5990010 5990011 5990012 5990013 5990014 5990015 S990016 S990017 S990018 S990019 S990020 S990021 S990022 S990023 S990024 S990025 S990026 S990027 S99002S S990029 S990030 S990031 S990032 S990033 S~ S990035 S990036 S990037 S990038 S990039 S990040 S990041 S990042 S990043 S~ S990045 S990046 S990047 S990048 S990049 1008 CT11231·2·2·1-M-1 1007 CT11231 ·2·2·1-M-2 1008 CT11231·2·2·1-M-3 1009 CT11231·2·2·1..,._. 101 O CT1 1231 ·2·2· 1 -M-5 CIRAD409 O.Sabana6 O.Sabana 10 1011 CT1 1231·2·2·1-M-6 1012 CT9899-12-6-3P·1-+M-1 1013 CT9899-12-6-3P·1-4-M·2 101. CT9899·12-6-3P-1-4-M-3 1015 CT9899-12-6-3P-1-4-M-4 1016 CT9899-12-6-3P-1-4-M-5 1017 CT9899-12-6-3P·1-4-M-6 1018 CT9907·5-3·1P-3-1-M·1 1019 CT9907·5-3-1P-3-1-M·2 1020 CT9907·5-3-1P-3-1-M·3 1021 CT9907-5-3-1P-3-1-M-4 1022 CT9907·5-3-1P·3·1·M·5 1023 CT9907·5-3-1P-3-1-M-6 1 02• CT11632-3-3-M-1 1025 CT11632·3-3-M·2 1026 CT11632·3-3-M-3 1027 CT11632·3·3-M-4 1028 CT11632-3-3-M-5 1029 CT11632-3·3-M-6 1 030 CT11891-3-3-3-M-1 1 031 CT11891-3-3-3-M-2 1032 CT1 1 891 -3-3-3-M-3 1033 CT11891 ·3-3-3-M-4 1~ CT11891·3-3-3-M·5 1035 CT11891-3-3-3-M-6 1036 CT1 1231 ·2·2·1-3-M-1 1037 CT1 1231·2·2·1-3-M·2 1038 CT11231·2·2·1-3-M-3 1 039 CT11231·2·2·1-3-M-4 1 a.o CT1 1 231 ·2·2· 1 -3-M-5 1041 CT1 1231·2·2·1-3-M-6 1 042 CT1 1 891 -3-3-3--M-1 1043 CT1 1891 -3-3-3-M-2 1 ~ CT1 1 891 --3-3-3-M-3 1 045 CT1 1 891-3-3-3-M-4 1046 CT1 1891 ·3-3-3-M-5 1047 CT1 1891 -3-3-3-M-6 1 046 CT1 3366-a-2-M-1 1049 CT13366-8·2-M·2 1050 CT13366-8·2-M-3 1051 CT13366-8·2-M-4 1052 CT13366-8·2·M·5 1053 CT13366-a-2-M-6 10M CT13366-9-3-M·1 3 5 3 3 3 5 3 2 2 5 1 5 2 1 5 2 2 5 2 5 2 3 5 2 1 5 2 2 5 2 2 5 5 5 2 5 5 5 5 5 5 5 5 5 3 3 3 3 3 2 3 5 3 2 5 2 5 2 2 5 2 5 5 5 5 5 3 3 2 2 3 3 3 2 50% 85 82 86 85 83 66 85 90 80 85 80 83 83 80 82 79 80 80 82 80 82 91 90 90 90 90 90 63 64 63 63 63 63 78 78 n 80 n 78 64 64 63 64 64 63 76 70 68 70 75 70 76 5 5 5 3 3 3 3 3 3 3 5 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 1 3 3 3 3 3 3 3 3 3 3 3 3 3 3 1 1 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 \ Nbr. 50 51 52 53 54 55 56 S1 56 59 eo 81 62 63 M 65 66 67 68 69 70 71 72 73 74 75 76 n 78 79 eo 81 82 63 84 65 86 87 68 89 90 91 92 93 94 95 96 97 98 99 100 101 Fleld Nbr. 11188A SV90050 $990051 $990052 5990053 8990054 $990055 S990056 S990057 S990056 5990059 5990060 5990061 5990062 5990063 5990064 5990065 T1 T2 T3 5990066 5990067 5990068 5990069 1055 1056 1057 1056 1058 1060 1061 1062 CTt~i-3-M-2 CT1~&-3-M-3 CT1~9-~ CT1~ CT1~ CT1~9-5-M-1 CT1 ~9-5-M-2 CT1~5-M-3 1063 CT13366-9-5-M-4 1064 CT1~9-5-M-6 1065 CT13370-2·1-M-1 1066 CT13370-2-1 -M-2 1067 CT13370-2·1-M-3 1066 CT13370..2·1-M-4 1069 CT13370..2-1-M-5 1070 CT13370-2-1-M-6 CIRAD409 O.Sabana6 O.Sabana 10 1071 CT13370-3-4-M-1 1072 CT13370..3-4-M·2 1073 CT13370..3-4-M-3 1 07 4 CT13370-3-4-M-4 5990070 1075 CT13370-3-4-M·5 CT13366-9·5-M-5 CT13370..3-4-M~ CT13371-5-1-M-1 CT13371·5-1-M-2 CT13371-5-1-M-3 CT13371 ·5·1 -M-4 CT13371-5-1-M-5 CT13371-5-1-M-6 CT13382-8-3-M-1 CT13382-8·3-M·2 CT13382-8-3-M-3 CT13382-8-3-M-4 CT13382-8-3-M-5 CT13382-8-3-M-6 CT13382-9-M-1 CT13382-9-M-2 CT13382·9-M-3 CT13382-9-M-4 CT13382·9-M-5 CT13382·9-~ CT13569-5-1-M-1 5990071 1076 5990072 ton 5990073 1 078 5990074 1079 5990075 1060 5990076 1081 59900n 1082 5990078 1083 5990079 1084 5990080 1 085 5990081 1086 5990082 1067 5990083 1068 5990064 - 1069 5990065 1 090 5990086 1091 5990087 1092 5990088 1 093 5990089 1094 5990090 1095 5990091 1096 5990092 5990093 5990094 5990095 5990096 5990097 5990098 5990099 5990100 5990101 1097 CT13569-5-1-M-2 1098 CT13569·5-1 -M·3 1099 CT13569·5-1-M-4 11 00 CT13569-5-1-M-5 1101 CT13569-5-1-M-6 1102 CT13569-5-5-M·1 1103 CT13569-5-5-M-2 11 04 CT13569-5-5-M-3 11 05 CT13569·5-5-M-4 1108 CT13569·5-5-M-5 Vg 5 5 5 3 3 3 3 5 81 2 4 4 3 2 3 2 3 5 2 5 4 3 2 3 2 5 5 3 5 7 81 5 3 2 7 2 5 5 5 5 7 7 7 3 3 3 3 1 3 3 5 5 5 5 5 5 5 1 2 3 2 3 2 2 2 2 2 1 3 3 4 2 2 3 2 2 1 2 5 1 2 3 3 5 2 5 3 3 5 2 5 5 2 5 2 7 2 5 7e 7e 70 72 M 78 7e 75 n 7e 78 80 80 80 80 80 M 86 91 81 85 86 84 89 81 80 63 82 80 78 80 78 80 78 80 80 80 82 85 81 82 82 82 84 86 85 63 86 89 85 90 84 65 84 82 LSc BS GD NBI 1 1 3 1 1 3 1 1 3 3 3 3 3 1 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 5 3 3 3 3 3 3 3 1 1 3 3 5 3 3 3 Nbr. FWd Nbr. Ortgln 11118 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 1MIA 5990102 8990103 8990104 5990105 5990108 S990107 5990108 5990109 S990110 5990111 5990112 5990113 5990114 5990115 5990116 5990117 S990118 5990119 T1 T2 T3 5990120 5990121 5990122 5990123 5990124 5990125 5990126 5990127 5990128 5990129 5990130 5990131 5990132 5990133 5990134 1107 CT13S69-~ 1108 CT13569-~1 1108 CT13S69-~2 1110 CT13S69-5-6-M-3 1111 CT13S69-5-6-M-4 1112 CT13569-5-6-M-5 1113 CT1~ 1114 CT1~1-M-1 1115 CT13569-8-1-M-2 1116 CT13569-8-1-M-3 1117 CT13569-8-1-M-4 1118 CT13569-8-1-M-5 1119 CT13569-8-1-M-6 1120 CT13569-8-2-M-1 1121 CT1~2-M·2 1122 CT13569-8·2-M-3 1123 CT13569-8-2-M-4 1124 CT13569-8·2-M-5 CIRAD409 0 .5abana6 O.Sabana 10 1125 CT13569-8-2-M-8 1126 CT1357o-1·3·M-1 1127 CT1357o-1-3-M·2 1128 CT1357o-1-3-M-3 1129 CT1357o-1-3-M-4 1130 CT1357o-1 -3-M-5 1131 CT1357o-1 -3-M-8 1132 CT1357o-3-2-M-1 1133 CT1357o-3-2-M-2 1134 CT1357o-3-2·M-3 1135 CT1357o-3-2-M-4 1136 CT1357o-3-2-M-5 1137 CT1357o-3-2-M-8 1138 CT1357o-3-3-M-1 1139 CT1357o-3-3-M-2 5990135 1140 CT1357o-3-3-M-3 CT1357o-3-3-M-4 CT1357o-3-3-M·5 CT1357o-3-3-M-8 5990136 - 1141 5990137 1142 5990138 1143 5990139 5990140 5990141 5990142 5990143 5990144 5990145 5990148 5990147 5990148 5990149 5990150 5990151 5990152 5990153 1144 CT1357o-13-4-M-1 1145 CT1357o-13-4-M·2 1146 CT1357o-13-4-M-3 1147 CT1357o-13-4-M-4 1148 CT1357o-13-4-M-5 1149 CT1357o-13-4-M-8 11 50 CT13571-1 2-3·M-1 1151 CT13571·12-3-M·2 1152 CT13571 -12-3-M-3 1153 CT13571-12·3-M-4 1154 CT13571-12·3-M-5 1155 CT13571·12-3-M-8 1156 CT13572·3-3-M·1 1157 CT13572-3-3-M·2 1158 CT13572-3-3-M-3 Vg 81 81 Fl LSc BS GD NBI 5 1 5 3 5 3 1 5 2 2 5 3 5 3 5 2 1 5 3 2 5 2 5 2 5 2 7 2 5 2 5 2 5 2 5 2 5 1 5 2 5 3 5 4 7 2 5 5 5 5 5 3 3 5 3 3 5 3 5 5 2 2 3 5 5 3 5 5 3 5 5 5 7 2 2 7 1 5 1 5 2 5 5 5 5 5 1 5 2 50% 84 85 81 se 87 80 78 82 80 83 81 78 78 82 78 81 82 66 89 90 67 86 78 81 88 83 78 80 81 79 85 81 80 80 81 82 80 80 87 80 80 80 79 80 83 87 87 66 87 84 85 85 73 88 66 3 3 3 3 1 3 3 3 1 3 3 3 3 1 3 3 3 3 3 1 1 3 3 3 1 3 1 3 1 Nbr. F1eld Nbr. Oñgln PedlgrM VI 81 81 R LSc BS OD NBI 1111M 1- 50% 154 5990154 1158 CT13572~ 5 88 155 S990155 1160 CT13572-3-3-M-5 5 70 158 S990158 1181 CT13572~ 5 1 68 1 Vg •lllgcr, 1!11 • 1M! blelt; l!l 2 • lell blelt; A • flowering; L8C • lell ICIId; 8S • llrown ~ Nl!l • nedc blelt; Gel • gnin cllcoioiiiiiOIL Table l Selected lines from the conventional brading project La libertad experimental station, 1999A Nbr. Fleld Nbr. Oñgln Pedlg .... Vg 81 81 F1 LSc BS OD NBI 1111M 1111118 50% S990009 1014 CT9899-12~· 1-4-M-3 5 2 3 83 3 3 2 5990009 1014 Cf9899.12-6-3P· 1-4-M-3 5 2 3 83 3 3 3 5990025 1030 CT1 1 891-3-3-3-M-1 3 83 3 4 5990029 1034 CT11891-3-3-3-M-5 3 2 83 3 5 5990034 1039 CT11231·2·2·1·3-M-4 5 2 90 3 6 5990035 1040 CT11231·2·2·1-3-M-5 5 n 3 7 5990036 1041 CT11231·2·2·1-3-M~ 5 78 8 5990037 1042 CT11891-3-3-3-M-1 5 64 9 5990041 1046 CT11891-3-3-3-M-5 1 64 10 5990058 1063 CT13386-9-5-M-4 5 2 n 1 11 5990072 1on CT13370-~ 7 80 3 12 5990090 1095 CT13382-9-M-6 5 64 3 13 5990110 1115 CT13569-6-1-M-2 5 2 90 14 5990120 1125 CT13569-6-2-M~ 5 86 15 5990152 1157 CT13572·3-3-M-2 5 68 16 5990154 1159 CT13572-3-3-M-4 5 69 TClble Z. Evcluation of Unes introduced from WAR[)A lA libertad experimental station, 1999A Nbr. Fleld Nbr. Orlgln Pedlg ... Vg 81 8 1 Fl LSc 8S GD NBI 1SIIIA 11118 50% 1 S910001 1552 w A845().1-8-P-82-2-1-1-M 5 2 78 2 S910002 1553 WAB450-1-B.P-82·2·1·2-M 5 78 3 S910000 1~ W A845().1-8-P-82·2·1-3-M 5 78 4 S910004 1555 WA845().1-8-P-82·2·14-M 5 78 1 5 S910005 1556 WA845().1-8-P-82·2·1·5-M 5 78 3 8 S910006 1557 WAB450-1-B-P-82·2·1~ 5 77 7 S910007 1556 WAB450-II-1-2.P50-HB-1-M 5 74 8 S910008 1559 WAB450-11·1·2-P50-HB-2-M 5 74 9 S910009 1560 WAB450-11·1·2.P50-HB-3-M 7 2 77 10 S910010 1561 WAB450-11·1·2.P50-HB-4-M 5 76 11 S910011 1562 WAB450-11·1 ·2-P50-HB-5-M 5 74 12 S910012 1563 WAB450-II-1-2·P50-HB-6-M 5 74 13 S910013 1564 WA8450-I-8-P·78-HB-1-M 5 70 14 S910014 1565 WA8450-1-B·P-78-HB-2-M 3 68 15 S910015 1566 WA8450-1-8-P-78-HB-3-M 3 68 16 S910016 1567 WA8450-1-B·P·78-HB-4-M 3 70 17 S910017 1568 WA8450-1-B-P-78-HB-5-M 3 68 1 T1 CIRAD409 5 66 3 T2 O.Sabana6 3 2 69 3 T3 O.Sabana 10 5 91 3 18 S910018 1569 WA8450-I-B-P-78-HB~ 5 69 19 S910019 1570 WAB450·1·B·P·57·3-1-1-M 3 75 20 S910020 1571 WAB450-1-B·P-57-3-1·2·M 3 74 3 21 S910021 1572 WAB450·1·B·P·57·3-1-3-M 5 75 22 S910022 1573 WA8450-1-B-P-57 -3-1-4-M 5 75 23 S910023 1574 WA8450-1-B-P·57 -3-1-5-M 3 75 24 S910024 1575 WAB450-1-B·P-57-3-1-&M 5 74 25 S910025 1576 WA8450-I·B-P-133-HB-1-M 5 2 77 26 S910026 1567 WA8450-l·B-P-&2-1 -&M 5 76 27 S910027 1568 WA8450-16·2-BL1 ·DR2-1-M 7 73 28 S910028 1589 WA8450-16-2·BL1·DR2-2-M 5 70 29 S910029 - 1590 WA8450-16-2-BL 1·DR2·3-M 5 73 30 S910030 1591 WA8450-16-2-BL1·0R24-M 7 74 31 S910031 1592 WA8450-16-2-BL 1·DR2·5-M 5 73 32 S910032 1593 WA8450-16-2-BL1 ·DR2~ 5 69 33 S910033 1594 WA8450-16·2-BL 1-0V1-1-M 5 69 34 S910034 1595 WA8450-16-2-BL1 -0V1-2-M 5 69 35 S910035 1598 WAB450-16·2-BL 1-DV1 -3-M 5 70 38 S910038 1597 W AB450-16-2-BL 1-0V14-M 5 70 Vg • vigor, Bl 1 • leal blast; Bl 2 • leal blast; A • llowering; LsC • leal scalcl; BS • brown spot; Nll • nec:1c biMI; Gel • pn ~- Table 2. Selected linc íntroduccd from WAR[)A 1.4 libertad cxpuirncntaJ station, 1999A Nbr. F1ekl Nbr. Ortgln Yg 81 81 R LSc BS QD N8l 1MA 50% S910035 15118 W~16-2-8L1-0V1-3-M 5 70 Table 3. Orym glabcrrima acccssions introduud from WAR[)A 1.4 libertad cxpuirncntaJ station, 1999A Nbr. F1eld Nbr. Orlgln ~ Yg 81 81 R LSc as QD NBI 1181A 111118 ~ 1 5910037 6331 TOO 3 101 2 5910038 5980TOG 3 104 3 5910039 5810TOG 5 89 4 5910040 5486TOG 3 120 Vg • 'llgor; 81 1 • 1M1 blut; 81 2 • IMI blast; R • ftowenng; l.liC • IMI acald; BS • brown 11101: NBI • ned< blut; Gel • QtWn cbco1ota1ion. TClble 4. Evaluation of F3 progenies from /Mdaga.scar (CIRA~/FOFIFA) LA libertad experimental station, 1999A Nbr. F1eld Nbr. Origln Pedigi'M Vg 81 81 F1 LSc es 00 NBI 1MA 1iMB 50% 1 5930001 1913 PRA729-ME 5 1 trT 2 5930002 1917 PRA768-ME 7 3 3 67 7 3 5930003 1918 PRA 769-ME 7 75 3 4 5930004 1923 PRA 770-ME 7 69 3 5 5930005 1924 PRA 771-ME 7 1 M 3 6 5930006 1934 PRAm-ME 7 2 66 3 3 7 5930007 1940 PRA 774-ME 7 58 8 5930006 1~ PRA775-ME 7 69 1 9 5930009 1988 PRA 736-ME 5 76 3 1 10 5930010 1991 PRA 737-ME 5 76 3 11 5930011 1999 PRA738-ME 5 73 3 12 5930012 2007 PRA 742-ME 7 62 13 5930013 2011 PRA735-ME 5 63 3 14 5930014 2012 PRA750-ME 5 67 1 15 5930015 2013 PRA 745-ME 7 69 3 16 5930016 ~J16 PRA746-ME 7 62 3 17 5930017 2023 PRA 747-ME 7 63 18 5930018 2025 PRA 748-ME 7 63 19 5930019 2026 PRA 749-ME 5 63 20 5930020 2031 PRA753-ME 5 57 21 5930021 2056 PRA764-ME 5 63 22 5930022 2058 PRA 762-ME 3 72 1 23 5930023 2059 PRA763-ME 5 63 3 24 5930024 2060 PRA765-ME 5 71 Vg • vioor; Bl 1 • IN! blast; B1 2 • IN! blast; A • tlowerin¡¡; LsC • IN! scald; BS • brown 1P0t NBI • nedc blast: Gd • grwn cücolorDOn. CHAPTER III POPULATION BREEDING FOR SAVANNA RICE 1 . INTRODUCTION 2. POPULATION BREEDING 2.1 . LINE DEVELOPMENT from RECURRENT POPULATIONS 2. 1 . 1 . Generation SO • Population PCT -11 \0\0\2 • Population PCT -4 \SA \3\1 2. 1. 2. Generation 52 • Populations PCT -5\PHB\1 \O,PHB\1,PHB\1; PCT -A \PHB\1 \O,PHB\1,PHB\1andPCT4\PHB\1 \1 ,PHB\1 ,PHB\1 • Population PCT -4\SA\2\1 • Population PCT -11 \0\0\1 2 .1 . 3. Generation 54 • Populations PCT -5\PHB\1 \O,PHB\1 PCT -A\PHB\1 \O,PHB\1 and PCT -4\PHB\1 \1,PHB\1 • Population PCT -4\SA\1 \1 2 .1 . 4 . Generation 56 • Populations PCT -5\PHB\1 \O,PCT -A\PHB\1 \0, and PCT -4\PHB\1 \1 2 .1 . 5 Generation 58 • Populations PCT -5\0\0\0, PCT -A\0\0\0, and PCT-4\0\0\1 • Population PCT -4\0\0\1 >52 • Populations PCT-5\0\0\0 >53, PCT-A \0\0\0 >53 and PCT-4\0\0\1>53 2.1.6. Advanced generations • Population CNA-IRAT5 and CNA-IRAT4 2.1.7. Upland line Registration 2.1.8. Line dispatch to NAR5 2.2. POPULATION MAINTENANCE through RECOMBINATION 2.3. POPULATION ENHANCEMENT 2.3.1. Recurrent 5election Based on 52 Line Evaluation: • Population PCT -4\0\0\1 2. 3. 2. Mass Recurrent 5election for Both 5exes for "Hoja Blanca", Blast, and Major Agronomic Traits: • Populations PCT -4\0\0\1, PCT-A \0\0\0, and PCT-5\0\0\0 2. 3. 3. Enhancement of the population PCT -11 2. 4 . REGI5TERING POPULA TION5 2.5. 5PECIAL 5TUDY CHAPTER III POPULATION BREEDING FOR SAVANNA RICE 1. INTRODUCTION The upland rice population breeding project, using recurrent selection, aims at adapting, developing and selecting tropical japonica gene pools and populations. The major characteristics we bred for are: • Tolerance of soil acidity • Resistance to diseases; rice blast (Pyricu/aria grisea Sacc.) • Resistance to pests, mainly rice plant hopper ( Tagosodes orizicolus) • Good grain quality (translucent, long-slender grain) • Early maturity (total cycle about 115 days) 2 . POPULA TION BREEDING The activities we report here were conducted during two seasons at two experiment stations. • Off season (1998 B): October 1998 - March 1999 at CIAT Palmira - Valle, "Palmira Experiment Station" (PES) • Cropping season (1999 A): April - September 1999 at Villavicencio - Meta, "La Libertad Experiment Station" (LES). Soil and climatic characteristics of LES experimental station are presented in the tables 1, and 2. 2.1. LINE DEVELOPMENT from RECURRENT POPULATIONS During the enhancement of gene pools and populations through recurrent selection, fertile plants are selected. This selection is the starting po int for the development of promising fixed lines for variety release and/or potential parents for our regional partners (Argentina, Brazil, Bolivia, Venezuela and the Caribbean through CRID Net). 2 .1 . 1 . Generation SO The first step in the development of fixed lines is the selection of individual fertile plants. ~j / - • opulation PCT -11 \0\0\2 (Secon cyc e o recom ma 1on \2\) Cropping season 1999 A Nineteen (19) individual fertile plants were selected. Table 3. During 1999 B the 51 generation will be grown off-season at PES and evaluated for tolerance to the Hoja Blanca Virus. {_/J. opu~tion ·P~\SA~ (Thir recom ination cycle \3\ after one selection for acid soil \SA\) Cropping season 1999 A Sixty-one (61) individual fertile plants were selected. Table 4. During 1999 B the 51 generation will be grown off-season at PES and evaluated for tolerance to the Hoja Blanca Virus. 2 .1 . 2. Generation 52 The generation 52 comes from fertile SO plants selected at LES during the cropping season 1998 A. The generation 51 was advanced off-season during 1998 B at PES. • Populations PCT -5\PHB\1 \O,PHB\1,PHB\1 PCT -A \PHB\1 \O,PHB\1 ,PHB\1; and PCT -4\PHB\1 \1 ,PHB\1,PHB\1 (Third cycle of recurrent selection for leaf blast P and Hoja Blanca HB) Cycles During 1998 A, we selected a total of 107 ferti/e SO plants (30, 24 and 53 plants respective/y). During 1998 B, the 107 lines 51 were advanced off-season at PES Cropping season 1999 A The 107 lines 52 were evaluated and 3 selected (3i'o) in two populations (no selection was made in PCT-5\PHB\1\0,PHB\1,PHB\1) (table 5). In each selected line a different number of individual plants was harvested. During 1999 8, the 13 lines (2 families of 6 lines and 1 family of 1 line) will be advanced off-season and evaluated for tolerance to the Hoja Blanca Virus at PES. 7 .. ~ee52 / 1 ~.,._ , ) /) ~ C . A J ¡/)A-(. /l /'1 1.:_? 1(-. f ¡;o te fe )=:>ti~ ' V , -. Cycles l • ' . ,_ , . 1 .-1 Al ( t: tJ ~A Gb U : ! . f3 U.J a ?'k:?.. S?---/ /_. e · n- During 1996 A, we started enhancing the population PCT-4 through 52 fine eva/uation. We took advantage of the 1996 52 fine tria/ to se/ect fines and individual fertile plants for fine development. From 152 52 fines evaluated, we se/ected 19 (12.5%) and 74 individual plants, based on plant and grain type, and grain yie/d potentia/. During 1996 S, the 53 generation was grown off-season at PES and the 54 seeds were sent toLES to grow the 54 generation during 1997 A. During 1997 A, from the 74 54 fines evaluated, 16 were selected (22%). In each selected fine, we harvested 6 indiv1dual plants. During 1997 S, the 96 55 fines (16 fammes of 6 fines) were grown at PES. Cropping Season 1998 A From the 96 fines 56 evaluated, 14 were selected (14.6%). In each selected fine 6 fertile plants were harvested During 1998 B, the 84 lines S? (14 families of 6 lines) were grown at PES. Two families were discarded. Cropping season 1999 A The 72 lines 58 selected (12 families of 6 lines) were evaluated, and 6 were selected (8.3i'o) at LES (table 12). In each selected line, 6 individual plants were harvested. During 1999 B, the 36 lines (6 families of 6 lines) will be grown off-season at PES. • Populations PCT-5\0\0\0 >53, PCT-A \0\0\0 >53 and PCT-4\0\0\1>53 (Basic populations. Plant selection in 53 Lines at PES, 1996 B) . ( ? ~ 1 ¿ ~ ~ f ~ ';) 6 n t~~i) A// ~ /t! t.;.. e Cycles , .... · , 1 ¿ -.."'1-'n ...4 1 1 1 ¡1/ / (;: ¡../ t;. t. e,. ,_.. ~ _,V .. n" 1 • '" • r .A c;t,t.) e"~ ) e , J - . ..,. During 1996 8, at PES, we selected 12 individual fertile plants, with suitab/e characteristics, from 53 fines. The 54 seed was sown during 1997 A at LES During 1997 A, from the 12 54 fines evaluated, only 3 were se/ected in one population: • PCT-5\0\0\0 -- no selection • PCT-A \0\0\0 -- no selection • PCT-4\0\0\1-- 3 fines se/ected (75%) In each selected fine, 6 individual plants were selected During 1997 8, the 18 55 fines (3 families of 6 fines) were grown at PES. Cropping seoson 1998 A. The 18 fines 56 were evaluated at LES, and 2 (11. %) were selected During 1998 B, the 12 lines 57 (2 families of 6 lines) were grown off-season at PES. Cropping season 1999 A The 12 lines were evaluated and 1 was selected (8.3i'o) at LES (table 13). In each selected line, 6 individual plants were harvested. During 1999 B, the 6 lines (1 family of 6 lines) will be grown off-season at PES. 2.1 . 6. Advanced generations These generations are promising fixed lines that were selected from the first introduced populations from Brazil in 1992. • Populations CNA-IRAT 5 and CNA-IRAT A Cycles During 1995 8, at PES, we increased seed of 2 and 4 advanced fines selected from CNA-IRA T 5 and CNA -IRA T A, respective/y. During 1996 A, we observed these 6 fines at LES. From each of the 6 fines, we se/ected 5 individual plants. During 1996 8, we increased seed of the 30 plants at PESto set up a yie/d tria/ during1997 A. During 1997 A, a yield tria/ was conducted and analyzed. 8 fines presented a high yie/d potentia/ and good milling characteristics. Cropping Seasons 1998 A and 1999 A The best lines were used to set-up the regional INGER-LAC acid soil nursery called "Vivero Internacional de Observación para America Latina" (VIOAL suelos ácidos, table 14). 2 .1. 7. Upland line Registration CIAT does not register lines. When a specific line does well in a given country, the national institution of that country may decide to name and release it for commercial cultivation. CIRAD has a mechanism by which breeders can register specific material. The line is named CIRAD and is also given a "local synonym", if it is the result of a collaborative work. Cycles During 1996, two advanced lines--CNA-IRAT 5 \SA \0\3>127-2-M-2-M and CNA-IRAT A \SA \0\3>1-M-2-M-4-M, se/ected from the populations CNA-IRAT 5 and CNA-IRA T A--were proposed for registration in the CIRAD rice catalog. They are registered as CIRAD 410 and CIRAD 411, respective/y. During 1997, the results of a yie/d tria/ showed that 3 fines were very promising. They were se/ected from two recurrent populations. They were remitted to INGER LACto be part of the VIO AL tria/ for acid soil condition. During 1998, we apply for their registration in the CIRAD rice catalog. In 1999, these lines were seed increased at PES for further distribution to regional NARS. 2 . 1. 8 . Line dispatch to NARS During 1999, we dispatched seed samples (58 generation) to our main collaborators in LAC and abroad, for evaluation and selection. Samples of 385 lines were shipped to: Argentina Brazil Bolivia Venezuela The Caribbean Cuba China Universidad de Tucumán EMBRAPA Arroz e Feijoo CIAT- Bolivia , Santa Cruz de la Sierra FONAIAP CRID Net IIA FCRI/YAAS - Yunnan Province CATAS - Hainan Province The results of the evaluation and local selection will be presented next year. 2 . 2. POPULATION MAINTENANCE through RECOMBINATION Cycles Unti/ now, the upland popu/ations were maintained under irrigated conditions at Palmira. But, results obtained in Madagascar under similar condition show that a possib/e genetic drift toward an increased frequency of Indica plant type may occur in the population. Such a drift can be explained by a more effective cross-pollination among genotypes with Indica background We must remember that the male-stertle fine used to build up populations is an Indica fine (IR 36 male-stertle mutant). l>uring the 1996 A cropping season, we decided to maintain and 1ncrease seed of upland populations under savanna conditions. We ma1nta1ned the follow1ng 6 populations: CNA-IRA T 5/ 0/ 4, CNA -IRA T A/ 0/2, CNA-IRA T P/ 1/ 1, PCT-A \0\0\0, PCT-5\0\0\0, and PCT-4\0\0\1. A 11 male-sterile plants were identified, harvested Individual/y, and their seeds mixed 1n equa/ proportions. Fertile plants were a/so harvested Individual/y and their seeds mixed 1n equal proportions. The populations were sent to CIA T Palmira, and stored 1n a co/d chamber unttl further use by the project or requested by regional NARS breed1ng programs. During 1997, 1998 and 1999, no population maintenance was made, enough seed is stored in the CIA T cold chamber. 2.3. POPULATION ENHANCEMENT The CIA T rice project emphasizes pre-breeding activities. Then we concentrate on the enhancement of populations to be distributed to NARS. The strategy is to develop and enhance gene pools and populations for well-targeted traits. They are therefore used as reservoir of promising lines and/or potential parents to be developed by national breeding programs. In the first 3 years of the project, we concentrated on introducing, characterizing and selecting germplasm from Brazil (former joint project between EMBRAPA Rice and Beans). From 1995 onward, we focus on enhancing the selected populations and developing new ones. 2. 3 .1. Recurrent Selection Based on 52 Line Evaluation • Population PCT -4\0\0\1 Cycles During 1995 A, at LES, 159 SO fertile plants were selected During 1995 8, the 51 generation was grown at PES. During 1996 A, we started the first recurrent selection cycle: • Evaluation: 152 fines of 52 and 2 checks (Oryzica Sabana 6 and CIRAD 409) were e,valuated and se/ected at LES under the ''Augmented Blocks" statistica/ design. • Selection: Results of the 52 tria/ were analyzed and 53 52 fines were selected • Recombination: In 1996 8, at PES, remaining seeds from the SO plants from which originated the selected 52 fines were mixed and grown to develop the recombined enhanced population. • Identification: The enhanced recombined population was identified as PCT-4\SA \1\1. During 1997 A, the population PCT-4\SA \1\1 was grown at LES to go through a second selection cycle. Harvest of Male-Sterile Plants. Male-sterile plants were harvested individual/y and their seeds mixed in equal proportions to complete the second cyc/e of recombination of the population se/ected one time. The second cyc/e of recombination is identified as PCT-4\SA \2\1. Seed wi/1 be stored in the cold chamber for future use. Selection of Fertile Plants. A total of 155 SO plants were selected, anda sample of each SO seed was stored in the co/d chamber. During 1997 8, the 51 generation was grown at PES, and 52 seeds harvested. Cropping Season 1998 A From the 155 52 fines, 152 were evaluated during 1998 A at LES in a tria/ named "Augmented B/ocs of Federer" {BAF}, tab/e 5. With a selection index of 39.5% we selected the 60 best fines for recombination from the original SO se/ected plants. During 1998 B, the recombination was made at PES by the sowing of the balanced mixture of SO seed (equal proportion of seed of each SO plant) and harvest of the seeds produced by the mole sterile plants. The second cycle of recurrence was identified as PCT-4\SA\1\1, SA\1 Cropping season 1999 A Recombination The population PCT-4\SA \2\1 with one cycle of recurrence and recombined twice was grown at LES to obtain the third recombination identified as PCT-4\SA \3\1 Multi-site evaluation of 52's lines A set of 155 lines 52 from the first cycle of recurrence was remitted to Brazil (EMBRAPA Arroz e Feijao), Bolivia (CIAT Santa Cruz), and Venezuela (UNELLEZ) for evaluation and selection for line development. In Bolivia, 22 lines were selected and their progenies will be evaluated next year, for fixed line development. Selection of So fertile plants Individual selection of fertile plants (table 15) was made in the population PCT-4\SA\2\1 (see table 4, generation SO) 2.3.2 . Mass Recurrent Selection for Both Sexes for "Hoja Blanca", Blast, and Major Agronomic Traits • Populations PCT -4\0\0\1, PCT -A\0\0\0, and PCT -5\0\0\0 Cycles During 1995 A, at LES, we eliminated at the vegetative stage al/ plants showing symptoms of /eaf b/ast and HBV. At harvest, we selected male-fertile plants. Seeds produced by these plants were the result of fertilization with po/len produced by hea/thy fertile plants. We selected 102, 99, and 96 male-sterile plants from PCT-5\0\0\0, PCT-A \0\0\0, and PCT-4\0\0\1, respective/y, and their seeds were mixed in equal proportions. The first mass recurrent selection cycles (se/ection and recombination) were identified as PCT-5\PHB\1\0, PCT-A \PHB\1\0 and PCT-4\PHB\1\1, respective/y. During 1996 A, the seed mixture of each population with one mass recurrent se/ection cyc/e was grown at LES. To develop the second recurrent selection cyc/e, the same se/ection method as that used during1995 A was applied We selected 304, 341, and 442 healthy male-sterile plants, fertilized with po/len of fertile healthy plants, from PCT- 5\PHB\1\0, PCT-A \PHB\1\0, and PCT-4\PHB\1\1, respective/y, and mixed their seeds in equal proportions. The second mass recurrent se/ection cyc/es (selection and recombination) were identified as PCT-5\PHB\1\0,PHB\1, PCT-A \PHB\1\0,PHB\1, and PCT-4 \PHB \1\J,PHB 11, respective/y. During 1997 A, the seed mixture of each population with two mass recurrent se/ection cyc/es was grown at LES. To make the third recurrent selection cyc/e, the same selection method as that used during 1995 A and 1996 A was applied (al/ plants with symptoms of leaf blast and "hoja blanca" were eliminated during their vegetative stage). We selected 218, 253, and 165 healthy male-sterile plants, fertilized with po/len from fertile healthy plants, from PCT-5\PHB\1\0,PHB\1; PCT- A \PHB\1\0,PHB\1; and PCT-4\PHB\1\l,PHB\1, respective/y, and their seeds mixed in equal proportions. The third mass recurrent selection cyc/es (se/ection and recombination) are 1dentified as PCT-5\PHB\1\0,PHB\l,PHB\1; PCT-A IPHB 111 O,PHB 1 J,PHB 1 J; and PCT-4 \PHB 11 1 J,PHB 1 J,PHB 1 J. Cropping Season 1998 A Fourth Cycle of Recurrent Se/ection The seed mixture of each population with 3 mass recurrent selection cyc/es was grown at LES. To make the fourth recurrent selection cyc/e, the same selection method as that used during 1995 A, 1996 A and 1997 A was applied (al/ plants with symptoms of /eaf blast and "hoja blanca" were eliminated during their vegetative stage). We selected 180, 200, and 240 healthy male-steri/e plants, fertilized with po/len from fertile hea/thy plants, from PCT-5\PHB\1\0,PHB\1,PHB\1; PCT-A \PHB\1\0,PHB\1, PHB\1; and PCT-4\PHB\1\1,PHB\1, PHB\1 respective/y, and their seeds mixed in equal proportions. The fourth mass recurrent se/ection cyc/es (se/ection and recombination) are identified as PCT-5\PHB\1\0,PHB\1,PHB\1, PHB\1; PCTA \PHB\1\0,PHB\1,PHB\, PHB\1; and PCT-4\PHB\1\1,PHB\1,PHB\1, PHB\1. During 1998 B, after completed 4 cycles of recurrence, the enhanced populations were stored in the CIA T cold chamber at PES. They would be dispatched to LAC NARS as reservoir of genotypes for line development. Results of the enhancement • Total resistance to leaf blast The results of the enhancement of the populations PCT -4. PCT -A, and PCT -5, for total leaf blast resistance are presented in the following table. The original populations presented about 40-ro of susceptible plant. From the first cycle of selection on, a drastic reduction in the number of infected plants occurs. The use of mass recurrent selection on both sexes was efficient for the enhancement of the 3 populations. At the same time we selected for totalleaf blast resistance, we also selected for good agronomic characters. The enhanced populations will be used as reservoir for the development of fixed lines. Results of the enhancement of the 3 populations PCT-5, PCT-A y PCT-4 for total leaf blast resistance. Cycles Year of PCT-5 PCT-A PCT-4 evaluation Basic Population 1995 47.8 * 35.3 42.7 First Cycle 1996 1.5 l. O 0.5 Second Cycle 1997 3.7 3.3 4.5 Third Cycle 1998 0.3 0.2 0.1 * Percent of plants with leaf blast symptom • Resistance to the "Hoja Blanca" virus During the first semester of 1999 at PES, 107 lines 52 from the 3 populations after 3 cycles of recurrence were evaluated to Hoja Blanca in nurseries with high-infected insect pressure. The results of the evaluation are presented in the following table. The 52 evaluation of the enhanced populations showed that 97.2'o have resistant and intermediate reaction to Hoja Blanca. They presenta lower incidence of the disease than the lines from FEDEARROZ and ICA. The enhanced populations can be considered as good reservoirs for the development of resistant fixed lines by LAC NARS. Results of the enhancement of the 3 populations PCT-5, PCT-A and PCT-4, for resistance to Hoja Blanca. Reaction to Hoja Blanca Resistant Intermediate Susceptible (1-9 scale) (1-3) (5) (7-9) Enhanced Populations 54.2* 42.9 2.8 FEDEARROZ Lines 59.1 30.6 10.2 ICA Lines 51.4 4.0 44.4 IRRI Lines 5.6 4.6 89.7 Colombia 1 (R check) 90.3 9.7 0.0 Blue Bonnet (S check) 0.0 3.8 96.2 CICA 8 (I check) 0.0 86.4 13.6 • Percent of plants with Hoja Blanca symptom 2 . 3 . 3 . Recombination of the population PCT -11 The new population was developed during 1997 and 1998. Cropping season 1999 The second cycle of recombination was completed at LES. Fertile plants of the second recombination (population PCT-11\0\0\2) were selected (table 15), (see table 3, generation SO). 2. 4. REGISTERING NEW POPULATIONS In 1999, one new germplasm (see Appendix 4) was registered, on request from our partner in China. • PYN-1 Japonica population for upland-hillsides ecosystem (FCRI/YAAS, Yunnan Province- China). 2.5. SPEaAL STUDY. Genetic Progress, Population PCT -4 Principal objective Master Degree Thesis of Miss Yolima Ospina at the "Universidad Nacional de Palmira". Specific objective Evaluation of genetic progress for acid soil tolerance and different agronomic characteristics like; flowering time, plant height and grain yield, through recombination cycles Genetic progress after one selection cycle and effect of different recombination cycles. Material Evaluation of 51 lines coming from: • The original population identified as PCT-4 \0\0\0 • The f irst selection cycle for acid soil tolerance, followed by one, two and three cycles of recombination identified as PCT-4 \SA \1\1 , PCT-4 \SA \2\1 y PCT-4 \SA \3\1 respectively. The study was developed at "La Libertad" Experimental Station Villavicencio- Meta, Colombia, during the first semester of 1999. 51 lines were obtained at the "CIA T Palmira" Experimental Station during off- season 1998. Method Two contrasted plots for soíl acidity (applícation of 300 and 3,000 kg/ha of lime) were used for the study. The statistical design was the Augmented Federer blocks composed by 51 from the four population and 6 checks (3 susceptible - CICA 8, CICA 9 y Oryzica Llanos 5- and 3 tolerant- Oryzica Sabana 6, Oryzica Sabana 10 y CIRAD 409- to soil acidíty). Data were collected and are currently processed. Table 1. Soil analysis of the experimental ~ite (Lote Loma 5) • la libertad • Experimental Station, Colombia, 1999 cropping season. p Depth O .. M Brayn pH Al Ca Mg K C.I .E 8 Zn Mn Cu (cm) (1o) (ppm) Meq./100gr ppm 1 0-20 3.6 13.1 4 .7 3.12 0.62 0 .38 0 .21 3 .55 0.17 0 .36 6.86 0 .44 20-40 2.8 2.8 4 .9 3.22 0 .20 0 .23 0.10 3.06 0.41 0.21 5.15 0 .37 2 0-20 4.2 7.6 4.7 3.22 0.54 0 .30 0.16 3.25 0 .27 0 .28 6.90 0 .40 20-40 3.0 1.8 4 .9 2.97 0.22 0.22 0 .06 2.75 0.18 0.17 4 .22 0.32 Table 2. Climatic characteristics of the 1999 cropping season at • La Libertad· Experimental Station, Colombia. Fe 17.86 10.30 15.05 7.61 Characteristics April May June July Aug. Sep. Total/ Average Rainfall (mm) 530.0 411.0 483.0 165.0 138.6 122.5 1850.1 Temperature (maximum •C) 29.0 29.7 28.9 28.9 29.4 30.0 29.31 Temperature (minimum •e) 21.8 21.2 21.4 20.9 22.0 23.1 21.73 Relative humidity (1.) 84 85 87 86 81 82 84.16 Radiation (Cal/cm2 ) 461.4 424.2 409.3 416.9 407.1 410.2 Al Sat. (1.) 72.10 86.00 76.50 87.66 Table3. SO fertile plants selected in the population PCT-11\0\0\2 La Libertad experimental station, 1999A Nbr. Fleld Nbr. Pedlgree 1999A 1 32 PCT-11\0\0\2>32 2 205 PCT-11\0\0\2>205 3 286 PCT -11 \0\0\2>286 4 432 PCT -11 \0\0\2>432 5 617 PCT -1 1 \0\0\2>617 6 623 PCT -11 \0\0\2>623 7 701 PCT-11\0\0\2>701 8 725 PCT -11 \0\0\2> 725 9 917 PCT-11\0\0\2>917 10 980 PCT-11\0\0\2>980 11 1062 PCT -11 \0\0\2> 1 062 12 1188 PCT-11\0\0\2>1188 13 1313 PCT-11\0\0\2>1313 14 1390 PCT-11 \0\0\2> 1390 15 1396 PCT-11\0\0\2>1396 16 1418 PCT-11 \0\0\2> 1418 17 1485 PCT -11 \0\0\2> 1485 18 1817 PCT-1 1\0\0\2>1817 19 1947 PCT-11\0\0\2>1947 Table 4. SO fertile plants selected in the population PCT-4\SA\3\1 La Libertad experimental station, 1999A Nbr. Fleld Nbr. Pedigree Nbr. Field Nbr. Pedlgree 1ft9A 1999A 10 PCT-4\SA\3\1>10 32 1034 PCT -4\SA\3\1 >1034 2 49 PCT -4\SA\3\1>49 33 1058 PCT -4\SA\3\1>1058 3 83 PCT -4\SA\3\ 1 >83 34 1082 PCT -4\SA\3\1>1082 4 94 PCT -4\SA\3\1>94 35 1084 PCT -4\SA\3\ 1> 1 084 5 118 PCT -4\SA\3\1>118 36 1089 PCT -4\SA\3\1>1089 6 157 PCT -4\SA\3\1>157 37 1109 PCT -4\SA\3\1>1109 7 244 PCT -4\SA\3\ 1>244 38 1175 PCT -4\SA\3\1>1175 8 '157 PCT -4\SA\3\1 >257 39 1231 PCT -4\SA'J\1> 1231 9 318 PCT -4\SA\3\1>318 40 1241 PCT -4\SA\3\1>1241 10 404 PCT -4\SA\3\ 1>404 41 1296 PCT -4\SA\3\1>1296 11 426 PCT -4\SA\3\1>426 42 1307 PCT -4\SA\3\1>1307 12 484 PCT -4\SA\3\1>484 43 1327 PCT -4\SA\3\ 1> 1327 13 520 PCT -4\SA\3\ 1 >520 44 1334 PCT -4\SA'J\1>1334 14 524 PCT -4\SA\3\1 >524 45 1335 PCT -4\SA\3\ 1> 1335 15 543 PCT -4\SA\3\1>543 46 1345 PCT -4\SA'J\1>1345 16 613 PCT-4\SA\3\1>613 47 1377 PCT -4\SA\3\ 1> 1377 17 695 PCT-4\SA\3\1 >695 46 1378 PCT -4\SA\3\1> 1378 18 706 PCT -4\SA\3\1>706 49 1402 PCT -4\SA\3\1>1402 19 772 PCT -4\SA\3\1>772 50 1413 PCT -4\SA\3\1>1413 20 829 PCT -4\SA\3\1>829 51 1441 PCT -4\SA\3\1>1441 21 836 PCT-4\SA\3\1>836 52 1489 PCT-4\SA\3\1>1489 22 841 PCT-4\SA\3\1>841 53 1631 PCT -4\SA'J\1>1631 23 843 PCT-4\SA\3\ 1>843 54 1701 PCT -4\SA\3\1>1701 24 846 PCT -4\SA\3\1>846 55 1704 PCT -4\SA\3\ 1 > 1704 25 866 PCT -4\SA\3\1>866 56 1781 PCT-4\SA\3\1>1781 26 869 PCT -4\SA\3\ 1>869 57 1818 PCT -4\SA\3\1>1818 27 904 PCT-4\SA\3\1>904 58 1839 PCT -4\SA\3\ 1 > 1839 28 930 PCT -4\SA'J\1>930 59 1850 PCT -4\SA\3\1 >1850 29 936 PCT -4\SA\3\1>936 60 1867 PCT -4\SA\3\ 1> 1867 30 983 PCT -4\SA\3\1>983 61 1917 PCT -4\SA\3\1>1917 31 1009 PCT -4\SA\3\1> 1009 Table 5. Evaluation of S21ines from the populations PCT-4\PHB\1\1,PHB\1,PHB\1> PCT-5\ PHB\ 1 \O,PHB\1,PHB\1> and PCT-A \ PHB\ 1 \ O,PHB\1,PHB\1> La libertad experimental station, 1999A Nbr. Aeld Nbr. Origln 19988 Pedlgree Vg 81 81 A LSC BS 00 N81 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 1999A 8920158 8920159 8920160 8920161 $920162 $920163 8920164 89.20165 8920166 8920167 8920168 T1 T2 T3 $920169 $920170 $920171 $920172 $920173 8920174 $920175 $920176 $920177 $920178 $920179 S920180 8920181 5920182 5920183 $920184 S920185 5920186 5920187 $920188 5920189 $920190 $920191 5920192 5920193 5920194 5920195 5920196 5920197 5920198 5920199 5920200 5920201 5920202 5920203 5920204 5920205 171 PCT·4\PHB\1\1,PHB\1,PHB\1>135-M 172 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>191-M 173 PCT-4\PHB\1\1 ,PHB\ 1,PHB\1>227·M 174 PCT·4\PHB\1\1,PHB\1 ,PHB\1>228-M 175 PCT·4\PHB\1\1,PHB\1,PHB\1>262·M 176 PCT·4\PHB\1\1,PHB\1 ,PHB\1>300.M 177 PCT·4\PHB\1\1,PHB\1,PHB\1>329-M 178 PCT·4\PHB\1\1,PHB\1,PHB\1>336-M 179 PCT-4\PHB\ 1\1 ,PHB\1 ,PHB\1>359-M 180 PCT·4\PHB\1\1,PHB\1,PHB\1>443-M 181 PCT·4\PHB\1\1,PHB\1,PHB\1>455-M CIRAD409 O.Sabana6 O.Sabana 10 182 PCT·4\PHB\1\1,PHB\1,PHB\1>472·M 183 PCT·4\PHB\1\1,PHB\1,PHB\1>479-M 184 PCT-4\PHB\1\1 ,PHB\1,PHB\1>507-M 185 PCT·4\PHB\1\1,PHB\1 ,PHB\1>509-M 186 PCT-4\PHB\1\1 ,PHB\1,PHB\1>541-M 187 PCT·4\PHB\1\1,PHB\1 ,PHB\1>572·M 188 PCT·4\PHB\1\1,PHB\1,PHB\1>583-M 189 PCT·4\PHB\1\1,PHB\1,PHB\1>584·M 190 PCT·4\PHB\1\1,PHB\1 ,PHB\1>585-M 191 PCT·41PHB\1\1,PHB\1,PH8\1>587·M 192 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>603-M 193 PCT·4\PHB\1\1,PHB\1,PHB\1>614·M 194 PCT·4\PHB\1\1,PHB\1,PHB\1>650.M 195 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>692·M 196 PCT·4\PHB\1\1 ,PHB\1,PHB\1>816-M 197 PCT·4\PHB\1\1,PHB\1,PHB\1>869-M 198 PCT·4\PHB\1\1,PHB\1,PHB\1>894-M 199 PCT·4\PHB\1\1,PHB\1,PHB\1>1047·M 200 PCT·4\PHB\1\1,PHB\1,PHB\1>1062·M 201 PCT-4\PHB\1\1 ,PHB\1,PHB\1>1093-M 202 PCT-4\PHB\1\1 ,PHB\1,PHB\1>1112·M 203 PCT·4\PHB\1\1,PHB\1 ,PHB\1>1155-M 204 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>1234-M 205 PCT·4\PHB\1\1,PHB\1,PHB\1>1255-M 206 PCT·41PHB\1\1 ,PHB\1,PHB\1>1264-M 207 PCT-4\PHB\1\1 ,PHB\1,PHB\1>1298·M 208 PCT·4\PHB\1\1,PHB\1,PHB\1>1308-M 209 PCT·4\PHB\1\1,PHB\1,PHB\1>1335-M 210 PCT·4\PHB\1\1,PHB\1,PHB\1>1373·M 211 PCT-4\PHB\1\1 ,PH8\1,PH8\1>1376·M 212 PCT-4\PHB\1\1 ,PHB\1 ,PHB\1>1377·M 213 PCT·4\PHB\1\1,PHB\1,PHB\1>1382·M 214 PCT·4\PHB\1\1,PHB\1,PHB\1>1398·M 215 PCT·4\PHB\1\1,PHB\1,PHB\1>1487·M 216 PCT·4\PHB\1\1,PHB\ 1 ,PHB\1>1515-M 217 PCT-4\PHB\1\1 ,PHB\1,PHB\1>1545-M 220 PCT·4\PHB\1\1,PHB\ 1,PHB\1>1734·M 5 5 3 2 50% 66 70 66 5 3 2 75 5 2 73 5 3 74 3 3 3 85 5 2 2 74 5 2 64 5 2 82 5 3 2 75 7 67 3 4 3 88 7 3 2 90 3 3 2 83 5 2 75 7 3 2 64 5 3 3 78 3 2 64 5 2 3 5 2 3 2 5 75 60 64 70 71 7 2 2 76 5 5 66 65 3 2 2 63 3 2 69 5 2 81 5 2 3 64 5 5 3 3 2 3 66 62 75 68 67 5 2 68 5 4 4 85 5 2 63 5 2 68 7 5 5 72 5 4 4 69 5 4 4 68 5 5 4 64 5 3 2 68 7 4 3 74 5 3 2 65 7 2 2 73 7 4 4 83 7 3 3 64 5 4 3 79 3 3 3 3 3 3 3 3 3 3 3 5 3 3 5 3 3 3 3 5 3 3 3 3 5 3 5 5 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ·1 ·1 : Nbr. Aeld Nbr. 49 50 51 52 53 5<4 55 56 57 58 59 60 61 62 63 6<4 65 66 67 68 69 70 71 72 73 7<4 75 76 n 78 79 ao 81 82 a3 8<4 as a& 87 M 89 90 91 92 93 9<4 95 96 97 98 99 100 1999A 8920206 5920207 5920208 5920209 5920210 5920211 5920212 8920213 892021<4 5920215 5920216 8920217 5920218 8920219 8920220 8920221 5920222 T1 T2 T3 8920223 592022<4 5920225 5920226 $920227 $920228 8920229 8920230 5920231 5920232 5920233 592023<4 8920235 5920236 5920237 5920238 8920239 8920240 8920241 5920242 5920243 59202<4<4 8920245 59202<46 5920247 59202<48 8920249 5920250 5920251 5920252 5920253 5920254 5920255 5920256 5920257 Origln 1ms Pedlgree 221 PCT-4'PHB\1\1,PHB\1 ,PHB\1>1735-M 222 PCT-<4'PHB\1\1,PHB\1 ,PHB\1>1~M 223 PCT·<4\PHB\1\1,PHB\1,PHB\ 1>1895-M 22<4 PCT -4'PHB\1\1,PHB\1,PHB\1>1912-M 225 PCT -4'PHB\1\1,PHB\1 ,PHB\1>19<45-M 226 PCT·5'PHB\1'D,PHB\1 ,PHB\1>126-M 227 PCT·5\PHB\1'D,PHB\1,PHB\1>163-M 228 PCT·S'PHB\1'D,PHB\1,PHB\1>192-M 229 PCT ·S'PHB\ 1\D,PHB\ l ,PHB\1>226-M 230 PCT-5\PHB\1'D,PHB\1,PHB\1>23G-M 231 PCT·5'PHB\1'D,PHB\1,PHB\1>2<42-M 232 PCT·5'PHB\1'D,PHB\1 ,PHB\1>25a-M 233 PCT·5\PHB\1'D,PHB\1 ,PHB\1>331-M 23<4 PCT·5\PHB\1'D,PHB\1 ,PHB\1>33a-M 235 PCT-5\PHB\1'D,PHB\1 ,PHB\1>38<4-M 236 PCT·5'PHB\1'D,PHB\1 ,PHB\1>385-M 237 PCT·5\PHB\1'D,PHB\1 ,PHB\1>439-M CIRAD-409 O.Sabana6 O.sabana 10 238 PCT·5'PHB\1'D,PHB\1 ,PHB\ 1><4<4<4-M 239 PCT·5'PHB\1'D,PHB\1,PHB\1>515-M 240 PCT ·5'PHB\ 1\D,PHB\ 1,PHB\ 1>579-M 2<41 PCT ·S'PHB\ 1\D,PHB\ 1,PHB\ 1 >59o-M 2<42 PCT·5\PHB\1'D,PHB\1 ,PHB\1>618-M 243 PCT·5\PHB\1'D,PHB\1,PHB\1>619-M 2<4<4 PCT ·5\PHB\ 1\D,PHB\ 1,PHB\1>652-M 2.45 PCT·5\PHB\1'D,PHB\1 ,PHB\1>730-M 2<46 PCT ·5\PHB\ 1\D,PHB\ 1,PHB\ 1 >8<42-M 2<47 PCT·5\PHB\1'D,PHB\1,PHB\1>1037·M 2<48 PCT-5\PHB\1'D,PHB\1 ,PHB\1>1129-M 249 PCT-5\PHB\1'D,PHB\1 ,PHB\1>13<41-M 250 PCT-5'PHB\1'D,PHB\1 ,PHB\1>1591 -M 251 PCT·5'PHB\1'D,PHB\1,PHB\1>1651·M 252 PCT-5'PHB\1'D,PHB\1 ,PHB\1>1661·M 253 PCT-5\PHB\1'D,PHB\1 ,PHB\1>167G-M 25<4 PCT·5\PHB\1'D,PHB\1,PHB\1>1as3-M 255 PCT·5\PHB\1'D,PHB\1 ,PHB\1:>1917-M 256 PCT-A\PHB\1'D,PHB\1 ,PHB\1:>253-M 257 PCT·A'PHB\1'D,PHB\1 ,PHB\ 1>397·M 258 PCT·A\PHB\1'D,PHB\1,PHB\1:>421-M 259 PCT ·A \PHB\ 1\D,PHB\ 1,PHB\ 1:>6<42-M 260 PCT·A\PHB\1'D,PHB\1 ,PHB\1>721-M 261 PCT·A\PHB\1'D,PHB\1 ,PHB\1>866-M 262 PCT·A\PHB\1\0,PHB\ 1,PHB\1>946-M 263 PCT-A\PHB\1'D,PHB\ 1,PHB\1>1058-M 264 PCT·A\PHB\ 1'D,PHB\ 1,PHB\1:>1194·M 265 PCT·A\PHB\ 1'D,PHB\1,PHB\1>1302·M 266 PCT·A\PHB\1'D,PHB\ 1,PHB\1:>1326-M 267 PCT·A\PHB\ 1\0,PHB\1,PHB\1>13<47·M 268 PCT·A\PHB\1'D,PHB\1,PHB\1>1361·M 269 PCT·A\PHB\11D,PHB\1 ,PHB\1>1<4<47·M 270 PCT·A\PHB\ 1'D,PHB\1,PHB\1:>1631·M 271 PCT·A\PHB\1'D,PHB\1,PHB\1>1744-M 272 PCT-A\PHB\1'D,PHB\1,PHB\1>1802·M Vg 81 81 Fl LSc BS GD NBI 50'!1. 5 2 2 73 5 2 68 7 2 76 5 3 3 6<4 7 2 3 71 5 76 7 2 3 69 3 2 68 5 2 7<4 7 2 2 as 71 5 2 5 2 66 5 2 2 6<4 5 as 5 1 63 s 2 ao 5 2 n 5 6<4 5 • 2 87 5 3 89 5 7<4 5 72 5 3 3 69 5 2 68 5 70 5 6<4 7 2 n 7 66 7 69 7 73 7 2 73 7 73 5 76 5 72 5 2 69 5 69 5 3 66 9 2 n 7 67 5 78 5 2 67 7 2 n 7 82 7 7 7 7 7 69 69 2 74 2 76 3 76 7 3 78 7 67 5 2 2 71 5 65 5 64 5 66 7 72 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 5 3 3 3 3 5 3 3 3 3 3 3 3 3 Nbr. Fleld Nbr. Origln Pedlgree Vg 81 81 F1 LSc 8S GD N81 1999A 19988 50'!1. 101 5920258 273 PCT·A\PHB\1\0,PHB\l,PHB\1>1827-M 7 68 102 5920259 274 PCT ·A \PHB\1\0,PHB\1,PHB\1> 1834-M 7 66 103 5920260 275 PCT·A\PHB\1\0,PHB\1,PHB\1>1a5~ 5 2 66 104 5920261 276 PCT·A\PHB\1\0,PHB\l ,PHB\1>1889-M 7 4 4 n 3 3 105 5920262 2n PCT·A\PHB\1\0,PHB\1 ,PHB\1>1933-M 5 3 4 68 106 5920263 278 PCT·A\PHB\1\0,PHB\1 .PHB\1>1~ 5 2 n 3 3 107 5920264 279 PCT·A\PHB\1\0,PHB\1 ,PHB\1>2007·M 7 2 69 Vg • vtgor. 811 • lee! bleet; 812 • leal b iM1; Fl • llowellng; t..aC • lee! acald; BS • brown apot; N81 • nec:l< blut; Gd • graí'l cltcoloratlon. Table 5. Selected 52 lines from the two populations, PCT-4\PHB\1\1,PHB\1,PHB\1>, ood PCT-A \PHB\1\0,PHB\1,PHB\1> La libertad experimental station, 1999A Nbr. Fleld Nbr. Origin Pedlgree Vg 81 81 Fl LSc 8S GD NBI Nbr.Sel. 1999A 19988 50'!1. PL 5920175 188 PCT -4\PHB\1\1,PHB\1 ,PHB\1>583-M 3 60 6 2 5920176 189 PCT -4\PHB\1\1,PHB\1,PHB\1>584-M 5 2 64 6 3 5920259 274 PCT·A\PHB\1\0,PHB\1 .PHB\1> 1834-M 7 66 Table 6. Evaluation of S21ines from the population PCT-4\SA\2\1 La libertad experimental station, 1999A Nbr. FleldNbr. 1999A Origln 19988 Pedlgree Vg Bl Bl Fl LSc BS GO NBI 2 3 4 5 6 7 a 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 T1 T2 T3 89200a5 8920086 8920087 8920088 5920089 8920090 5920091 5920092 5920093 8920094 5920095 8920096 5920097 5920098 5920099 8920100 5920101 5920102 8920103 5920104 5920105 5920106 5920107 5920108 5920109 5920110 5920111 S920112 S920113 S920114 T1 T2 T3 S920115 S920116 S920117 S920118 S920119 S920120 S920121 S920122 S920123 S920124 S920125 S9201 26 S920127 S920128 S920129 S920130 S920131 96 97 CIRAD409 O .Sabana6 O .Sabana 10 PCT ·4\SA\2\1>38-M PCT-4\SA\2\1>43-M 98 PCT·4\SA\2\1>44·M 100 PCT·4\SA\2\1>113·M 101 PCT-4\SA\2\1>128-M 102 PCT-4\SA\2\1>172-M 103 PCT-4\SA\2\1>190-M 105 PCT-4\SA\2\1>275-M 106 PCT·4\SA\2\1>282·M 107 PCT·4\SA\2\1>287·M 108 PCT-4\SA\2\1>290-M 109 PCT·4\SA\2\1>31G-M 110 PCT-4\SA\2\1>317-M 111 PCT-4\SA\2\1>331-M 112 PCT-4\SA\2\1>333-M 113 PCT-4\SA\2\1>335-M 114 PCT-4\SA\2\1>375-M 115 PCT-4\SA\2\1>438-M 116 PCT·4\SA\2\1>473·M 117 PCT·4\SA\2\1>4n-M 118 PCT·4\SA\2\1>482·M 119 PCT·4\SA\2\1>521·M 120 PCT-4\SA\2\1>529-M 121 PCT-4\SA\2\1>589-M 122 PCT-4\SA\2\1>617-M 123 PCT-4\SA\2\1>626-M 124 PCT-4\SA\2\1>657-M 125 PCT·4\SA\2\1>664·M 126 PCT-4\SA\2\1>676-M 127 PCT·4\SA\2\1>6n·M CIRA0409 O.Sabana6 O.Sabana 10 128 PCT·4\SA\2\1>697·M 129 PCT-4\SA\2\1>731-M 130 PCT-4\SA\2\1>734-M 131 PCT-4\SA\2\1>764-M 132 PCT-4\SA\2\1>768-M 133 PCT·4\SA\2\1>n9-M 134 PCT-4\SA\2\1>786-M 135 PCT-4\SA\2\ 1>792-M 136 PCT-4\SA\2\1>805-M 137 PCT-4\SA\2\1>831-M 138 PCT-4\SA\2\1>855-M 139 PCT-4\SA\2\1>979-M 140 PCT-4\SA\2\1> 1020-M 141 PCT-4\SA\2\1>1158-M 142 PCT-4\SA\2\1>1181-M 143 PCT-4\SA\2\1>1190-M 144 PCT-4\SA\2\1>1196-M 5 3 4 5 3 S 5 5 2 5 5 5 4 5 3 5 2 5 4 5 2 5 4 5 2 5 3 5 2 5 2 5 2 3 2 5 4 5 2 7 5 5 5 4 5 3 5 3 3 2 3 3 5 3 5 2 3 3 3 4 5 4 5 3 3 3 5 2 7 3 5 2 5 3 5 2 5 2 3 2 S 2 5 4 5 3 7 3 7 4 5 3 S 2 3 2 3 2 3 3 1 S 4 3 3 2 4 2 2 3 2 S 4 2 3 2 2 3 3 3 3 2 2 3 2 2 2 3 2 50'!(, 64 86 92 59 72 68 70 72 67 66 63 76 65 69 72 65 66 64 75 62 64 64 84 81 66 66 65 59 82 71 82 63 62 64 86 89 73 72 71 74 69 64 65 80 n 63 64 71 83 75 62 64 71 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 5 5 3 3 3 3 3 3 S 3 3 3 3 5 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. Aeld Nbr. 1999A Origln 19988 Pedlgree Vg 81 81 Fl LSc BS GD N81 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 8920132 5920133 8920134 8920135 8920136 8920137 8920138 8920139 8920140 8920141 T1 T2 T3 8920142 8920143 8920144 8920145 5920146 5920147 8920148 5920149 5920150 8920151 8920152 5920153 5920154 5920155 8920156 8920157 145 PCT-4\SA\2\1>1198-M 146 PCT-4\SA\2\1> 1225-M 147 PCT·4\SA\2\1>1234·M 148 PCT-4\SA\2\1>1235-M 149 PCT·4\SA\2\1>1238·M 150 PCT·4\SA\2\1>127G-M 151 PCT-4\SA\2\1>1300-M 152 PCT-4\SA\2\1>1306-M 153 PCT-4\SA\2\1>1326-M 154 PCT·4\SA\2\1>1343·M CIRAD 409 O.Sabana6 O.Sabana 10 155 PCT-4\SA\2\1>1416-M 156 PCT-4\SA\2\1>1417-M 157 PCT·4\SA\2\1>1492·M 158 PCT-4\SA\2\1>1513-M 159 PCT-4\SA\2\1>1559-M 160 PCT·4\SA\2\1>1564·M 161 PCT·4\SA\2\1>1605·M 162 PCT-4\SA\2\1>1615-M 163 PCT·4\SA\2\1>1669·M 164 PCT-4\SA\2\1>1699-M 165 PCT·4\SA\2\1>1728·M 166 PCT·4\SA\2\1>1794·M 167 PCT·4\SA\2\1>1807·M 168 PCT-4\SA\2\1> 1836-M 169 PCT-4\SA\2\1>1923-M 170 PCT-4\SA\2\1>1985-M 5 4 5 3 5 2 5 3 5 2 5 2 3 2 5 3 5 5 3 3 3 5 4 5 3 5 4 5 3 2 5 4 7 3 5 3 5 4 3 3 3 2 5 4 5 2 7 5 5 2 7 3 5 2 3 2 Vg • vigor; Bl 1 a leal bias!; 812 •leal blast; Fl • flowertng; LaC • leal aceld; BS • brown apot; NBl • neck blast Od • graln dlscoloratlon. Table 6. Selected 52 lines from the population PCT -4\SA\2\1 La Libertad experimental station, 1999A 3 3 2 3 2 4 3 2 3 2 2 2 5 2 50% 66 74 66 72 68 68 64 65 71 61 64 85 118 77 71 60 72 70 64 67 66 68 82 65 80 85 75 78 61 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. Aeld Nbr. 1999A Origln 19988 Pedlgree Vg Bl Bl Fl LSc BS GD N81 Nbr.Sel. 2 3 4 5 8920125 8920130 8920147 8920149 5920152 138 PCT-4\SA\2\1>855-M 5 3 143 PCT-4\SA\2\1>1190-M 3 2 160 PCT-4\SA\2\1>1564-M 5 3 162 PCT-4\SA\2\1>1615-M 3 3 165 PCT-4\SA\2\1> 1728·M 5 2 2 2 2 60% PI. 64 64 64 66 65 6 3 6 6 6 Table 7. Evaluation of 52 line$ from the population PCT-11\0\ 0\1 La libertad experimental station, 1999A Nbr. A eldNbr. 1i9iA Ortgln 111988 Pedlgree Vg 81 81 A LSc BS 00 N81 2 3 4 5 6 7 a 9 10 11 12 13 14 15 16 17 1a 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 5920001 5920002 5920003 59~ 5920005 5920006 5920007 5920008 5920009 89'20010 5920011 5920012 5920013 5920014 5920015 5920016 5920017 S92001a 5920019 5920020 5920021 5920022 5920023 5920024 5920025 5920026 5920027 T1 T2 T3 S92002a 5920029 5920030 S920031 5920032 5920033 5920034 5920035 5920036 5920037 5920038 5920039 S920040 5920041 5920042 5920043 5920044 5920045 5920046 S920047 592004& 5920049 2 3 4 5 6 7 a 9 11 12 13 14 15 16 17 19 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 41 42 43 44 45 46 47 48 49 50 51 52 54 PCT-11'-t!Nl\1>42-M PCT-11\0\0\1>51-M PCT-1 1'-t!Nl\1>52-M PCT-11'-t!Nl\1>73-M PCT ·11'-t!Nl\ 1>84-M PCT-11'-t!Nl\1>a7-M PCT-11'-t!Nl\1>110.M PCT-11\0\0\1>112-M PCT-11\0\0\1>134-M PCT ·11 '-t!Nl\ 1> 15o-M PCT ·11'-t!Nl\ 1> 155-M PCT-11\0\0\1>166-M PCT-11\0\0\1>224-M PCT · 11'-t!Nl\ 1>225-M PCT -11\0\0\ 1>228-M PCT ·11\0\0\ 1>242-M PCT- 11\0\0\1>256-M PCT ·11\0\0\ 1>26&-M PCT-11\0\0\1>309-M PCT-11'-t!Nl\1>314-M PCT-11'-t!Nl\1>317-M PCT-11\0\0\1>375-M PCT-11\0\0\1>376-M PCT-11'-t!Nl\1>386-M PCT- 11'-t!Nl\1>405-M PCT-11\0\0\1>438-M PCT-11'-t!Nl\1>493-M CIRAD409 O.Sabana6 O.Sabana 10 PCT · 11'-t!Nl\ 1>509-M PCT- 11\0\0\1>513-M PCT-11\0\0\1>516-M PCT· 11'-t!Nl\1>522·M PCT·11'-t!Nl\1>~M PCT-11\0\0\1>566-M PCT-11'-t!Nl\1>576-M PCT-11'-t!Nl\1>621-M PCT-11'-t!Nl\1>657-M PCT-11\0\0\1>727-M PCT- 11\0\0\1>733-M PCT -11\0\0\1 > 734-M PCT-11\0\0\1>781·M PCT -11\0\0\ 1> 782-M PCT-11'-t!Nl\1>801-M PCT- 11\0\0\1>a10.M PCT-11\0\0\1>816-M PCT-11'-t!Nl\1>831-M PCT-11'-t!Nl\ 1>1141-M PCT-11\0\0\1>866-M PCT-11\0\0\1>877-M PCT-11\0\0\1>905-M 5 5 5 5 3 5 3 3 3 5 5 5 3 5 5 5 3 5 3 5 5 5 5 5 5 7 3 5 3 5 5 5 5 3 3 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 2 2 3 3 4 4 3 3 3 5 3 2 2 3 2 2 2 2 4 3 3 5 2 2 4 3 4 2 2 2 3 2 4 5 3 4 3 3 5 3 4 2 2 50'!1. a7 84 71 a7 68 68 70 3 71 4 74 7a 2 68 2 73 3 72 72 69 84 72 68 73 68 65 75 2 68 66 3 66 4 as 66 2 65 2 86 2 92 72 66 a2 69 2 72 83 70 a1 1 69 3 73 4 as 3 as 2 68 3 73 4 68 4 73 3 67 4 71 2 69 2 86 66 69 3 3 3 3 3 3 3 3 3 3 5 3 5 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. Fleld Nbr. 1999A Ortgln 19988 Pedlgree Vg 81 81 F1 LSc BS 924-M PCT ·11'D\O\ 1>943-M PCT-11\0\0\1>953-M PCT-11'D'D\1>1013-M PCT ·11'D'D\ 1 > 1 050-M CIRA0409 O.Sabana6 O.Sabana 10 PCT· 11'D'D\1>1083-M PCT-11'D'D\1> 1092-M PCT · 11'D\O\ 1> 1138-M PCT·11'D\0\1>1184-M PCT-11'D\0\1> 1196-M PCT-11\0\0\1>1199-M PCT -11\0\0\1 > 1308-M PCT-11\0\0\1> 131Q-M PCT-11\0\0\1> 1337-M PCT-11'D\0\1>1372·M PCT-11\0\0\1>13114-M PCT ·11\0\0\ 1 > 1486-M PCT-11\0\0\1>152o-M PCT- 11\0'D\1>1526-M PCT- 11\0\0\1>1537-M PCT-11'D'D\1>1600-M PCT-11'D'D\1>1606-M PCT-11\0\0\1> 1628-M PCT- 11\0\0\1>1629-M PCT ·11'D\O\ 1 > 1636-M PCT-11\0\0\1> 1660-M PCT-11\0\0\1> 167Q-M PCT-11'0\0\1>1681-M PCT · 11'D\O\ 1> 1709-M PCT-11'D\0\1>17n-M PCT · 11 \0\0\ 1> 178o-M PCT· 11'D\0\1> 179o-M PCT-11\0\0\1>1814-M PCT-11\0\0\1>1959-M PCT-11'0\0\1>1961-M 7 3 7 5 5 5 5 7 5 5 5 3 5 3 3 3 3 5 5 5 5 5 5 5 7 5 5 5 3 5 5 5 7 5 5 5 5 5 4 4 3 4 50% 4 79 2 65 3 77 2 73 2 65 2 66 5 4 86 3 2 92 3 81 3 2 68 2 2 68 2 3 3 2 2 2 2 2 5 2 4 2 3 4 4 2 4 5 4 4 5 3 74 74 66 2 85 69 72 85 60 66 75 63 80 74 5 84 2 68 4 69 3 70 69 4 76 4 63 2 74 4 60 4 85 3 73 4 75 4 75 2 69 Vg • vigor. 811 • leal blast; 81 2 alea! blaat; Fl • flowerlng; u C • leal acald; BS • brown apot; NBI • neck blaet Od • graln dilcolorallon. Table 7. Selected 52 lines from the population PCT-11\0\0\2 La Ubertad experimental station 1999A 3 3 3 3 5 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. Fleld Nbr. 1999A Orlgln 19988 Pedlgree Vg 81 81 Fl LSc 8S GD NBI Nbr.Sel. 2 3 4 5920012 S920027 S920029 5920051 13 29 32 57 PCT-11\0\0\1>166-M PCT ·11'D\O\ 1 >438-M PCT-11\0\0\1>513-M PCT-11\0\0\1>943-M 5 7 3 3 5 50% PI. 2 73 4 85 66 2 65 3 3 4 6 6 6 Table 8. Evaluation of 54 lines from the population PCT -4 \PHB\ 1 \1,PHB\1,> La libertad experimental station, 1999A Nbr. Fleld Nbr. Ortgln 19988 Pedlgree Vg Ht 81 81 A LSc BS 00 NBI 2 3 4 5 8 7 a 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 28 27 28 29 30 31 32 33 34 35 11191JA S$.40001 S$.40002 5940003 S940()(U S$.40005 S$.40008 S$.40007 S94000a S$.40009 S940010 8940011 S940012 8940013 8940014 8940015 S940016 S940017 S940018 T1 T2 T3 S940019 8940020 S940021 S940022 S940023 S940024 S940025 S940028 S940027 S940028 S940029 5940030 8940031 S940032 8940033 8940034 S940035 280 PCT -4\PHB\1\1,PHB\1>46-M-1-M 281 PCT·4\PHB\1\1,PHB\1>46-M·2·M 282 PCT -4\PHB\1\1,PHB\1>46-M-3-M 283 PCT -4\PHB\ 1\1,PHB\ 1>46-M--4-M 284 PCT -4\PHB\1\1,PHB\1>46-M·5·M 285 PCT·4\PHB\1\1,PHB\1>46-M~·M 286 PCT --'\PHB\1\1,PHB\1>120-M-1-M 287 PCT--'\PHB\1\1,PHB\1>120-M-2-M 288 PCT --'\PHB\1\1,PHB\1>120-M-3·M 289 PCT-..\PHB\1\1,PHB\1>12G-M--4-M 290 PCT--'\PHB\1\1,PHB\1> 120-M-5-M 291 PCT--'\PHB\1\1 ,PHB\1>120-M~·M 298 PCT--'\PHB\1\1,PHB\1>1534-M-1-M 299 PCT-..\PHB\1\1,PHB\1>1534-M-2-M 300 PCT--'\PHB\1\1,PHB\1>1534-M-3-M 301 PCT·4\PHB\1\1,PHB\1>1534· M·4·M 302 PCT-4\PHB\1\1,PHB\1>1534· M-5-M 303 PCT -4\PHB\ 1\1,PHB\ 1> 1534-M-6-M CIRAD409 O .Sabana 6 O.Sabana 10 304 PCT-4\PHB\1\1,PHB\1>1537·M-1-M 305 PCT --'\PHB\1\1,PHB\1> 1537-M-2-M 306 PCT-4\PHB\1\1,PHB\1>1537· M·3-M 307 PCT-4\PHB\1\1,PHB\1>1537·M-4-M 308 PCT -4\PHB\1\1,PHB\1>1537·M-5-M 309 PCT-4\PHB\1\1,PHB\1>1537-M·6-M 310 PCT -4\PHB\1\1,PHB\ 1>1776-M-1-M 311 PCT-4\PHB\1\1,PHB\1>1776-M-2-M 312 PCT-..\PHB\1\1,PHB\1>1776-M·3-M 313 PCT·4\PHB\1\1,PHB\1>1776-M·4-M 314 PCT --'\PHB\1\1,PHB\1> 1776-M-5-M 322 PCT-A\PHB\1'4l,PHB\1>853-M-1-M 323 PCT·A\PHB\1'4l,PHB\1>853-M·2-M 324 PCT-A\PHB\1\0,PHB\1>853-M-3-M 325 PCT-A\PHB\ 1\0,PHB\1>853-M-4-M 326 PCT·A\PHB\1'4l,PHB\1>853-M-5-M 327 PCT ·A \PHB\ 1\0,PHB\ 1>853-M-6-M Vg • vigor; 81 1 • leal btaet; 812 a leal blaat Fl • llowertng; LaC •leal aceld; BS • brown apol; N81 • ned( blut; Od • graln dilc:oloratlon. 7 71 2 7 80 7 62 2 2 7 78 2 2 7 72 2 2 7 78 2 5 75 2 5 72 7 73 3 2 7 77 3 2 7 77 3 3 7 78 3 3 5 83 2 3 93 3 98 3 96 5 91 5 101 5 93 3 104 3 2 5 90 2 s as 7 82 7 84 9 93 7 93 2 7 96 7 76 5 78 2 5 79 2 5 80 2 5 83 2 7 93 3 7 90 5 97 5 96 7 93 5 97 50'!(, 62 69 67 71 71 68 60 59 63 62 62 63 62 66 64 62 64 64 66 as 88 62 63 69 62 63 75 63 64 64 64 66 64 68 64 66 66 67 3 3 5 3 3 3 3 5 3 3 3 3 3 3 3 4 3 5 3 5 3 Table 8. Selected 541ines from the population PCT -4\PHB\1\l,PHB\1> L4 Libertad experimental station, 1999A Nbr. Fleld Nbr. Origln Pedlgree Vg Ht Bl Bl A LSc BS 00 NBI 199U 19988 SO'll. S940008 287 PCT4\PHB\1\1,PHB\1>120-M-2-M 5 72 1 59 2 8940010 289 PCT 4\PHB\1\1 ,PHB\1> 120-M-4-M 7 n 3 2 62 3 3 8940013 298 PCT 4\PHB\1\1,PHB\1> 1534-M-1-M 5 83 2 62 • 8940016 301 PCT 4\PHB\1\ 1,PHB\1> 1534-M-4-M 3 96 62 3 5 S940019 304 PCT4\PHB\1\1,PHB\1>1537·M-1-M 5 as 62 6 S940023 308 PCT4\PHB\1\1,PHB\1>1537·M-5-M 7 93 2 63 7 8940026 311 PCT 4\PHB\1\1,PHB\1> 1n&-M-2-M 5 78 2 64 Table 9. Evaluation of S41ines from the population PCT-4\SA\1\1> La libertad experimental station, 1999A Nbr. 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Z7 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 AeldNbr. 1999A 5940036 8940037 S940038 5940039 59<40040 5940041 5940042 5940043 5940044 5940045 5940046 5940047 5940048 5940049 5940050 5940051 5940052 5940053 5940054 5940055 5940056 5940057 5940058 5940059 59400PQ 5940061 5940062 5940063 5940064 5940065 5940066 5940067 5940068 5940069 5 940070 5940071 5940072 T1 T2 T3 5940073 5940074 5940075 5940076 59400n 5940078 5940079 5940080 5940081 5940082 5940083 5940084 Origln 19988 1162 PCT-4\SA\1\1>45-M+M 1163 PCT-4\SA\1\1>45-M-2-M 1164 PCT-4\SA\1\1>45-M-3-M 1165 PCT-4\SA\1\1>45-M-4-M 1166 PCT-4\SA\1\1>45-M-5-M 1167 PCT-4\SA\1\1>45-M-6-M 1168 PCT-4\SA\1\1>95-M+M 1169 PCT-4\SA\1\1>95-M-2-M 1170 PCT-4\SA\1\1>95-M-3-M 1171 PCT-4\SA\1\1>95-M-4-M 1172 PCT-4\SA\1\1>95-M-5-M 1173 PCT-4\SA\1\1>95-M-6-M 1174 PCT-4\SA\1\1>109-M+M 1175 PCT-4\SA\1\1>109-M-2-M 1176 PCT-4\SA\1\1>109-M-3-M 11n PCT-4\SA\1\1>109-M-4-M 1178 PCT-4\SA\1\1> 109-M-5-M 1179 PCT-4\SA\1\1>109-M-6-M 1180 PCT-4\SA\1\1>126-M+M 1181 PCT-4\SA\1\1>126-M-2-M 1182 PCT-4\SA\1\1>126-M-3-M 1183 PCT-4\SA\1\1>126-M-4-M 1184 PCT-4\SA\1\1>126-M-5-M 1185 PCT-4\SA\1\1>126-M-6-M 1186 PCT-4\SA\1\1>147-M+M 1187 PCT-4\SA\1\1>147-M-2-M 1188 PCT-4\SA\1\1>147-M-3-M 1189 PCT-4\SA\1\1> 147-M·4·M 1190 PCT-4\SA\1\1>147-M-5-M 1191 PCT-4\SA\1\1>147-M-6-M 1192 PCT-4\SA\1\1>150-M-1 -M 1193 PCT-4\SA\1\1>150-M-2-M 1194 PCT-4\SA\1\1> 150-M-3-M 1195 PCT-4\SA\1\1>150-M-4-M 1196 PCT-4\SA\1\1>150-M-5-M 1197 PCT-4\SA\1\1>150-M-6-M 1198 PCT-4\SA\1\1>162-M-1-M CIRAD409 O.Sabana6 O .Sabana 10 1199 PCT-4\SA\1\1>162·M·2·M 1200 PCT·4\SA\1\1> 162· M·3·M 1201 PCT·4\SA\1\1> 162·M+ M 1202 PCT·4\SA\1\1> 162·M·5·M 1203 PCT·4\SA\1\ 1>162·M·6·M 1204 PCT-4\SA\1\1> 188·M+M 1205 PCT·4\SA\1\1>188·M·2·M 1206 PCT·4\SA\1\1>188·M·3·M 1207 PCT·4\SA\1\1>188·M·4·M 1208 PCT·4\SA\1\1>188·M·5·M 1209 PCT-4\SA\1\1> 188·M·6·M 1210 PCT-4\SA\1\1>193-M+M Vg Bl Bl Fl LSc BS GD NBI 50% 5 3 5 5 3 5 7 5 5 5 2 2 5 2 5 5 3 5 3 3 2 5 2 3 3 2 5 3 2 3 5 3 2 5 2 2 3 3 5 5 5 2 5 2 5 7 5 5 5 5 5 5 5 3 2 7 2 3 3 3 3 5 5 3 71 69 72 73 73 75 68 68 69 66 67 68 66 65 64 65 64 64 66 67 67 65 66 65 65 65 67 63 66 64 68 68 69 69 68 73 84 69 85 92 66 85 89 84 85 85 84 84 85 85 85 83 3 3 3 3 3 3 3 3 3 3 1 3 3 3 3 3 3 3 3 3 3 Nbr. 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 n 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 100 101 102 Fleld Nbr. 1999A 5940085 5940086 5940087 5940088 59-40089 5940090 5940091 5940092 5940093 5940094 5940095 5940096 5940097 5940098 5940099 Tl T2 T3 5940100 5940101 5940102 5940103 5940104 5940105 5940106 5940107 5940108 5940109 S940110 5940111 5940112 5940113 5940114 5940115 5940116 5940117 5940118 5940119 5940120 5940121 5940122 5940123 5940124 5940125 5940126 5940127 8940!28 5940129 5940130 5940131 5940132 5940133 5940135 5940136 5940137 Origln 19988 Pedlgree 1211 PCT-4\SA\ 1\1> 193-M-2-M 1212 PCT-4\SA\1\1>193-M-3-M 1213 PCT-4\SA\1\1> 193-M-4-M 1214 PCT-4\SA\1\1>193-M-5-M 1215 PCT-4\SA\1\1> 193-M-6-M 1216 PCT-4\SA\1\1>195-M-1-M 1217 PCT-4\SA\1\1> 195-M-2-M 1218 PCT-4\SA\1\1>195-M-3-M 1219 PCT-4\SA\ 1\1>195-M-4-M 1220 PCT-4\SA\1\1> 195-M-5-M 1221 PCT-4\SA\1\1> 195-M-6-M 1222 PCT-4\SA\1\1>223-M-1-M 1223 PCT-41SA\1\1>223·M·2-M 1224 PCT-4\SA\1\1>223-M-3-M 1225 PCT-4\SA\1\1>223-M-4-M CIRAD409 O.Sabana6 O.Sabana 10 1226 PCT-4\SA\1\1>223-M-5-M 1227 PCT-4\SA\1\1>223-M-6-M 1228 PCT-4\SA\1\1>230-M-1-M 1229 PCT-4\SA\1\1>230-M-2-M 1230 PCT-4\SA\1\1>230-M-3-M 1231 PCT-4\SA\1\1>230-M-4-M 1232 PCT-41SA\1\1>23D-M-5-M 1233 PCT-41SA\1\1>23D-M-6·M 1234 PCT-4\SA\1\1>236-M-1-M 1235 PCT-4\SA\1\1>236-M-2-M 1236 PCT-4\SA\1\1>236-M-3-M 1237 PCT-4\SA\1\1>236-M-4-M 1238 PCT-4\SA\1\1>236-M-5-M 1239 PCT-4\SA\1\1>236-M-6-M 1240 PCT-4\SA\1\1>260-M-1-M 1241 PCT-4\SA\1\1>260-M-2-M 1242 PCT-4\SA\1\1>260-M-3-M 1243 PCT-4\SA\1\1>260-M-4-M 1244 PCT-4\SA\1\1>260-M-5-M 1245 PCT-4\SA \1\ 1>260-M-6-M 1246 PCT-4\SA\ 1\1>261-M-1-M 1247 PCT-4\SA\1\ 1>261-M-2-M 1248 PCT-41SA\1\1>261·M·3·M 1249 PCT·41SA\1\1>261·M·4·M 1250 PCT-4\SA\1\1>261-M-5-M 1251 PCT-4\SA\1\1>261-M-6-M 1252 PCT-4\SA\1\1>279-M-1-M 1253 PCT-4\SA\1\1>279-M-2-M 1254 PCT-4\SA\1\1>279-M-3-M 1255 PCT·41SA\1\1>279·M·4·M 1256 PCT-4\SA\1\1>279-M-5-M 1257 PCT-4\SA\ 1\1>279-M-6-M 1258 PCT-4\SA\1\1>305-M-1-M 1259 PCT-4\SA\1\1>305-M-2-M 1261 PCT-4\SA\1\1>305-M-4-M 1262 PCT-4\SA\1\1>305-M-5-M 1263 PCT-4\SA\1\1>305-M-6-M Vg 81 81 Fl LSc BS GO NBI 3 3 3 5 5 5 5 5 5 5 7 5 7 7 5 5 3 3 7 2 5 7 7 7 7 7 7 9 9 9 9 9 9 9 9 9 9 7 9 9 9 9 9 7 7 9 9 7 9 7 7 2 7 2 5 7 7 9 2 7 2 2 2 50% 83 84 87 83 84 n 77 n n 78 81 80 80 80 79 65 87 87 80 82 82 84 82 82 84 85 85 82 85 83 83 83 66 67 65 66 67 64 67 68 64 64 65 64 63 66 67 65 63 66 68 69 68 75 73 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 1111 119 120 121 122 123 124 125 126 127 1211 129 130 131 132 133 134 135 136 137 1311 139 140 141 142 1-43 144 145 146 147 1411 149 150 151 152 153 154 Fleld Nbr. 19Q9A 89401311 8940139 5940140 8940141 8940142 59401-43 8940144 8940145 8940146 8940147 89401411 5940149 5940150 5940151 8940152 5940153 S940154 S940155 S940156 T1 T2 T3 S940157 59401511 5940159 S940160 8940161 5940162 5940163 8940164 S940165 8940166 5940167 S940168 S940169 S940170 5940171 5940172 5940173 S940174 5940175 5940176 S9401n S940178 S940179 S940180 59401111 59401112 59401113 59401114 S940185 59401116 S9401117 S9401SS 59401119 Orlgln 1.a PedlgrM 12$4 PCT-4\SA\1\1>306-M+M 1265 PCT-4\SA\1\1>306-M-2-M 1266 PCT-4\SA\1\1>306-M-3-M 1267 PCT-4\SA\1\1>306-~-M 1268 PCT-4\SA\1\1>306-M-5-M 1269 PCT-4\SA\1\1>306-M-6-M 1270 PCT-4\SA\1\1>341-M-1-M 1271 PCT -4\SA\1\1>341-M-2-M 1272 PCT-4\SA\1\1>341·M-3·M 1273 PCT-4\SA\1\1>341-M-4-M 1274 PCT·4\SA\1\1>341·M-5·M 1275 PCT·4\SA\1\1>341·M-6·M 1276 PCT-4\SA\1\1>440-M+M 12n PCT-4\SA\1\1>440-M-2-M 12711 PCT-4\SA\1\1>440-M-3-M 1279 PCT-4\SA\1\1>440-~-M 12110 PCT-4\SA\1\1>440-M-5-M 12111 PCT-4\SA\1\1>440-M-&·M 1262 PCT·4\SA\1\1>446-M-1·M CIAAD409 O.Sabana6 O.Sabana 10 12113 PCT·4\SA\1\1>446-M-2·M 12114 PCT-4\SA\1\1>446-M-3-M 12115 PCT-4\SA\1\1>446-M-4-M 12116 PCT-4\SA\1\1>446-M-5·M 12117 PCT·4\SA\1\1>446-M-6·M 12SS PCT-4\SA\1\1>500-M-1-M 12119 PCT·4\SA\1\1>500-M-2·M 1290 PCT·4\SA\1\1>500-M-3·M 1291 PCT·4\SA\1\1>500-M-4-M 1292 PCT-4\SA\1\1>500-M-5-M 1293 PCT-4\SA\1\1>500-M-&·M 1294 PCT·4\SA\1\1>503-M+M 1295 PCT·4\SA\1\1>503-M·2-M 1296 PCT·4\SA\1\1>503-M-3·M 1297 PCT-4\SA\1\1>503-~-M 12911 PCT-4\SA\1\1>503-M-5-M 1299 PCT-4\SA\1\1>503-M-&·M 1300 PCT·4\SA\1\1>516-M·1·M 1301 PCT·4\SA\1\1>516-M-2·M 1302 PCT-4\SA\1\1>516-M-:l·M 1303 PCT-4\SA\1\1>516-M-4-M 1304 PCT·4\SA\1\1>516-M·5·M 1305 PCT-4\SA\1\1>516-M-&·M 1306 PCT-4\SA\1\1>540-M+M 1307 PCT·4\SA\1\1>540-M·2·M 1308 PCT·4\SA\1\1>540-M-3·M 1309 PCT·4\SA\1\1>540-M·4·M 1310 PCT-4\SA\1\1>540-M·5·M 1311 PCT·4\SA\1\1>540-M-5·M 1312 PCT·4\SA\1\1>573-M·1·M 1313 PCT·4\SA\1\1>573·M·2·M 1314 PCT·4\SA\1\1>573-M·3·M 1315 PCT·4\SA\1\1>573·M-4·M Vg 81 81 Fl LSc BS GD N81 7 2 7 2 7 7 2 7 2 7 5 7 5 5 2 7 5 5 5 2 5 7 7 5 7 7 5 7 3 7 7 7 7 7 7 7 7 7 2 2 7 3 2 5 9 2 7 5 5 5 5 7 7 7 7 7 5 7 7 5 7 7 2 7 7 7 7 7 SCYII. 73 71 69 74 72 72 61 67 67 60 65 66 114 114 66 113 as 111 68 65 116 as 67 611 66 66 66 64 64 66 66 66 64 68 66 66 64 63 67 67 67 67 67 67 68 66 65 65 66 64 68 73 73 73 69 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 Fleld Nbr. 1999A $940190 5940191 S940192 5940193 5940194 S940195 5940196 5940197 5940198 5940199 $940200 S940201 S940202 8940203 8940204 S940205 5940206 8940207 S940208 5940209 S940210 S940211 S940212 S940213 T1 T2 T3 5940214 S940215 5940216 S940217 5940218 S940219 5940220 5940221 S940222 5940223 $940224 $940225 5940226 5940227 $940228 S940229 5940230 $940231 $940232 S940233 5940234 5940235 S940236 S940237 5940238 S940239 Ortgin 19988 Pedlgree 1316 PCT-4~\1\1>573-M-5-M 1317 PCT·4~\1\1>573-M-6·M 1318 PCT·4~\1\1>631·M+M 1319 PCT·4~\1\1>631·M-2·M 1320 PCT·4~\1\1>631·M-3·M 1321 PCT·4~\1\1>631·M-4·M 1322 PCT·4~\1\1>631·M-5·M 1323 PCT·4~\1\1>631·M-6·M 1324 PCT·4\SA\1\1>632·M+M 1325 PCT·4\SA\1\1>632·M·2·M 1326 PCT·4~\1\1>632·M-3-M 1327 PCT·4\SA\1\1>632·M-4·M 1328 PCT·4~\1\1>632·M·5·M 1329 PCT·4~\1\1>632·M-6-M 1330 PCT·4~\1\1>669-M+M 1331 PCT·4\SA\1\1>669-M·2·M 1332 PCT-4\SA\1\1>669-M-3-M 1333 PCT·4~\1\1>669-M-4·M 1334 PCT·4\SA\1\1>669-M·5·M 1335 PCT-4\SA\1\1>669-M-6-M 1336 PCT·4~\1\1>721·M-1·M 1337 PCT·4\SA\1\1>721·M·2·M 1338 PCT·4\SA\1\1>721·M·3·M 1339 PCT·4\SA\1\1>721·M-4·M CIRA0409 O.Sabana6 O.Sabana 10 1340 PCT·4\SA\1\1>721· M· 5·M 1341 PCT·4\SA\1\1>721· M-6·M 1342 PCT-4\SA\1\1>722-M-1-M 1343 PCT·4\SA\1\1>722·M-2·M 1344 PCT·4~\1\1>722·M-3·M 1345 PCT-4\SA\1\1>722· M·4·M 1346 PCT· 4\SA\1\1>722·M-5·M 1347 PCT-4\SA\1\1>722-M-6-M 1348 PCT·4\SA\1\1>763-M·1-M 1349 PCT·4\SA\1\1>763-M·2·M 1350 PCT-4\SA\1\1>763-M-3-M 1351 PCT-4\SA\1\1>763-M-4-M 1352 PCT·4\SA\1\1>763-M·5·M 1353 PCT-4\SA\1\1>763-M-6-M 1354 PCT-4\SA\1\1>813-M+M 1355 PCT·4\SA\1\1>813-M·2·M 1356 PCT·4\SA\1\1>813-M·3·M 1357 PCT-4\SA\1\1>813-M-4-M 1358 PCT·4\SA\1\1>813-M·5·M 1359 PCT· 4\SA\1\1>813·M·6·M 1360 PCT·4\SA\1\1>881·M+ M 1361 PCT·4\SA\1\1>881· M·2·M 1362 PCT·4\SA\1\1>881·M·3·M 1363 PCT· 4\SA\1\1>881·M·4·M 1364 PCT-4\SA\1\1>881-M-5-M 1365 PCT·4\SA\1\1>881·M·6·M Vg 81 81 A LSc BS 00 N81 7 7 7 7 7 7 7 5 7 5 7 7 7 7 7 7 7 7 7 7 7 7 7 7 5 5 3 2 5 2 5 7 7 2 7 2 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 5 5 5 5 5 5 50% 72 71 75 72 75 69 71 74 75 73 72 72 75 75 73 69 73 73 73 74 72 70 75 69 66 86 87 69 72 67 67 67 66 66 67 63 63 64 63 63 64 63 65 66 66 66 67 69 68 75 73 68 75 3 3 3 3 3 3 5 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbt'. 205 206 207 208 209 210 211 212 213 214 215 2 16 2 17 216 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 231 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 Fleld Nbt'. 1999A 5940240 5940241 5940242 5940243 5940244 5940245 5940246 5940247 5940248 S940249 5940250 S940251 S940252 S940253 S940254 5940255 5940256 S940257 S940258 5940259 S940260 S940261 5940262 5940263 5940264 5940265 S940266 S940267 T1 T2 T3 5940268 S940269 5940270 S940271 S940272 5940273 S940274 5940275 5940276 5940277 5940278 5940279 5940280 5940281 5940282 5940283 5940284 5 940285 5940286 5 940287 8940268 5940289 5940290 5940291 Orlgln 19988 Pedlgree 1368 PCT·4\SA\1\1>895-M+M 1367 PCT·4\SA\1\1>895-M·2·M 1368 PCT·4\SA\1\1>895-M-3·M 1369 PCT-4\SA\1\1>895-M-4-M 1370 PCT·4\SA\1\1>895-M·5·M 1371 PCT·4\SA\1\1>895-M~-M 1372 PCT·4\SA\1\1>910.M+M 1373 PCT·4\SA\1\1>910.M-2·M 1374 PCT·4\SA\1\1>910.M-3·M 1375 PCT•4\SA\1\1>910.M-4-M 1376 PCT· 4\SA\1\1>910.M·5·M 1377 PCT·4\SA\1\1>910.M~·M 1378 PCT-4\SA\1\1>948-M-1-M 1379 PCT ·4\SA\1\1>94&-M-2-M 1380 PCT· 4\SA\1\1>948-M·3·M 1381 PCT-4\SA\1\1>948·M·4·M 1382 PCT·4\SA\1\1>948-M·5·M 1383 PCT-4\SA\1\1>948-M~-M 1384 PCT·4\SA\1\1>975-M· 1·M 1385 PCT·4\SA\1\ 1>975-M·2·M 1386 PCT · 4\SA \1\ 1>975-M·3·M 1387 PCT-4\SA\1\1>975-M-4-M 1388 PCT· 4\SA\1\1>975-M·5·M 1389 PCT-4\SA\1\1>975-M~-M 1390 PCT·4\SA\1\1>982·M·1·M 1391 PCT-4\SA\1\1>982·M·2·M 1392 PCT· 4\SA\1\1>982·M·3·M 1393 PCT·4\SA\1\1>982· M·4·M CIRI\0409 O.Sabana6 O.Sabana 10 1394 PCT-4\SA\ 1\1>982·M·5·M 1395 PCT·4\SA\1\1>982·M~-M 1396 PCT·4\SA\1\1>1013·M·1·M 1397 PCT-4\SA\1\1>1013-M-2-M 1398 PCT·4\SA\1\1>1013-M·3·M 1399 PCT-4\SA\1\1> 1013-M-4-M 1400 PCT-4\SA\1\1>1013-M-5-M 1401 PCT-4\SA\1\1> 101H.1~-M 1402 PCT-4\SA\1\1>1036-M-1-M 1403 PCT-4\SA\1\1> 1036-M-2-M 1404 PCT-4\SA\1\1>1036-M-3-M 1405 PCT-4\SA\1\1>1036-M-4-M 1406 PCT-4\SA\1\1>1036-M·5·M 1407 PCT-4\SA\1\1>1036-M~-M 1408 PCT-4\SA\1\ 1>1044-M+M 1409 PCT-4\SA\1\1>1044·M·2·M 1410 PCT-4\SA\1\1> 1044·M·3·M 1411 PCT-4\SA\1\1>1044-M-4-M 1412 PCT-4\SA\1\1>1044-M-5-M 1413 PCT-4\SA\1\1>1044-M~-M 1414 PCT-4\SA\1\1>1108-M-1-M 1415 PCT-4\SA\1\1>1108-M-2-M 1416 PCT-4\SA\1\1>1108-M-3-M 1417 PCT-4\SA\1\1>1108·M·4·M Vg 81 81 A LSc BS 5 5 5 5 5 5 5 3 3 3 3 3 5 5 5 7 1 1 1 1 1 5 2 5 2 5 5 3 2 5 2 1 7 1 7 1 1 1 1 5 5 5 5 5 5 5 5 5 5 5 5 2 5 5 3 1 2 50% 73 68 70 69 69 71 73 73 75 71 72 73 69 68 68 61 66 72 66 66 69 61 66 63 65 72 66 66 63 86 87 73 75 76 78 80 81 74 n 68 73 73 68 70 72 72 10 73 72 12 73 73 71 68 68 3 3 3 3 3 3 3 3 3 1 N81 Nbr. 257 258 259 260 261 262 263 264 265 266 2fr7 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 Aeld Nbr. 1999A 5940292 5940293 5940294 T1 T2 T3 5940295 5940296 5940297 5940298 5940299 5940300 5940301 5940302 5940303 5940304 5940305 5940306 5940307 5940308 5940309 5940310 5940311 5940312 5940313 5940314 5940315 5940316 5940317 5940318 5940319 5940320 5940321 5940322 5940323 5940324 5940325 5940326 5940327 5940328 5940329 5940330 5940331 5940332 5940333 5940334 5940335 5940336 5940337 5940338 5940339 5940340 5940341 5940342 5940343 Origln 19988 Peclgr-.. 1418 PCT-4\SA\1\1>110&-M-5-M 1419 PCT-4\SA\1\1> 110&-M-6-M 1420 PCT-4\SA\1\1>1116-M-1-M CIRAD409 O.Sabana6 O.sabana 10 1421 PCT·4\SA\1\1>1116-M·2·M 1422 PCT-4\SA\1\1>1116-M-3-M 1423 PCT-4\SA\1\1> 1116-M·4·M 1424 PCT·4\SA\1\1>1 116-M·5·M 1425 PCT·4\SA\1\1>1116-M-6·M 1426 PCT·4\SA\1\1>1127·M+M 1427 PCT·4\SA\1\1>1127·M·2·M 1428 PCT·4\SA\1\1>1127·M·3·M 1429 PCT·4\SA\1\1>1127·M·4·M 1430 PCT·4\SA\1\1>1127·M·5-M 1431 PCT-4\SA\1\1> 1127-M-ó-M 1432 PCT-4\SA\1\1> 1156-M-1-M 1433 PCT-4\SA\1\1> 1156-M-2-M 1434 PCT-4\SA\1\1>1156-M-3-M 1435 PCT-4\SA\1\1>1156-M-4-M 1436 PCT-4\SA\1\1>1156-M-5-M 1437 PCT-4\SA\1\1>1156-M-6-M 1438 PCT-4\SA\1\1> 1199-M-1-M 1439 PCT-4\SA\1\1>1199-M-2-M 1440 PCT·4\SA\1\1>1199-M·3·M 1441 PCT-4\SA\1\1>1199-M-4-M 1442 PCT-4\SA\1\1>1199-M·S·M 1443 PCT-4\SA\1\1>1199-M-6-M 1444 PCT-4\SA\1\1>1206-M-t-M 1445 PCT-4\SA\1\1> 1206-M·2·M 1446 PCT-4\SA\1\1>1206-M-3-M 1447 PCT-4\SA\1\1>1206-M-4-M 1448 PCT-4\SA\1\1>1206-M-5-M 1449 PCT-4\SA\1\1>1206-M-6-M 1450 PCT-4\SA\1\1>1236-M-1 -M 1451 PCT-4\SA\1\1>1236-M-2-M 1452 PCT-4\SA\1\1>1236-M-3-M 1453 PCT-4\SA\1\1>1236-M-4-M 1454 PCT-4\SA\1.\1>1236-M-5-M 1455 PCT-4\SA\1\1> 1236-M-6-M 1456 PCT-4\SA\1\1>1241-M-t-M 1457 PCT-4\SA\1\1>1241·M·2·M 1458 PCT-4\SA\1\1>1241·M·3·M 1459 PCT· 4\SA\1\1>1241·M·4·M 1460 PCT-4\SA\1\1>1241 ·M·5·M 1461 PCT·4\SA\1\1>1241·M·6·M 1462 PCT·4\SA\1\1>1260·M+M 1463 PCT·4\SA\1\1>1260-M·2·M 1464 PCT-4\SA\1\1>1260·M·3·M 1465 PCT-4\SA\1\1>1260·M·4·M 1466 PCT-4\SA\1\1> 1260·M·5·M 1467 PCT-4\SA\1\1> 1260.M-6·M 1468 PCT·4\SA\1\1>1270·M+M 1469 PCT-4\SA\1\1>1270.M·2·M Vg Bl Bl A LSc: BS 00 NBI 7 7 7 7 1 7 3 2 7 2 5 5 5 5 5 7 7 7 7 7 7 7 5 5 7 7 2 7 7 5 7 2 7 2 7 5 5 5 5 5 5 5 5 5 5 5 5 7 7 7 7 7 5 3 3 2 7 2 7 7 2 7 7 5 7 7 7 50% 68 75 73 66 88 89 n 75 75 73 72 69 68 68 71 72 68 65 63 63 66 63 65 63 62 65 64 63 62 67 62 63 63 63 62 62 63 62 62 63 66 66 63 63 64 63 66 63 63 63 63 66 63 63 65 3 3 3 3 3 3 3 Nbr. 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 345 347 348 349 350 351 352 353 354 355 356 357 358 359 360 Aeld Nbr. 1999A 5940344 5940345 5940346 5940347 5940348 5940349 S940350 5940351 5940352 5940353 T1 T2 T3 5940354 5940355 5940356 S940357 5940358 5940359 5940360 5940361 5940362 5940363 5940364 5940365 5940366 5940367 S940368 5940369 S940370 5940371 5940372 5940373 5940374 $940375 S940376 5940377 S940378 S940379 S940380 5940381 5940382 5940383 S940384 $940385 S940386 S940387 S940388 $940389 $940390 5940391 $940392 $940393 $940394 $940395 Origln 19988 Pedlgree 1470 PCT-4\SA\1\1> 1270.M·3·M 1471 PCT-4\SA\1\1> 1270.M·4·M 1472 PCT·4\SA\1\1>1270.M·5·M 1473 PCT-4\SA\1\1>1270.M-6-M 1474 PCT-4\SA\1\1>1272-M-1-M 1475 PCT·4\SA\1\1>1272·M·2·M 1476 PCT-4\SA\1\1>1272-M-3-M 14n PCT·4\SA\1\1>1 272·M·4·M 1478 PCT-4\SA\1\1>1272-M·S·M 1479 PCT-4\SA\1\1>1272-M.O·M CIRA0409 O.Sabana6 O.Sabana 10 1480 PCT-4\SA\1\1>1475-M+M 1481 PCT-4\SA\1\1> 1475-M·2·M 1482 PCT·4\SA\1\1>1475-M·3·M 1483 PCT·4\SA\1\1>1475-M·4·M 1484 PCT-4\SA\1\1>1475-M-5-M 1485 PCT-4\SA\1\1>1475-M.O·M 1486 PCT-4\SA\1\1>1479-M-1-M 1487 PCT-4\SA\1\1>1479-M-2-M 1488 PCT-4\SA\1\1>1479-M-3-M 1489 PCT·4\SA\1\1>1479-M·4·M 1490 PCT-4\SA\1\1>1479-M-5-M 1491 PCT-4\SA\1\1>1479-M-6-M 1492 PCT-4\SA\1\1>1486-M-1-M 1493 PCT-4\SA\1\1>1486-M-2-M 1494 PCT-4\SA\1\1>1486-M-3-M 1495 PCT-4\SA\1\1>1486-M-4-M 1496 PCT-4\SA\1\1>1486-M-5-M 1497 PCT-4\SA\1\1>1486-M.O·M 1498 PCT-4\SA\1\1>1549-M+M 1499 PCT-4\SA\1\1> 1549-M-2-M 1500 PCT-4\SA\1\1> 1549-M·3·M 1501 PCT·4\SA\1\1>1549-M·4·M 1502 PCT-4\SA\ 1\1>1549-M-5-M 1503 PCT-4\SA\1\1>1549-M.O·M 1504 PCT-4\SA\1\1>1566-M-1-M 1505 PCT·4\SA\1\1>1566-M·2·M 1506 PCT-4\SA\1\1>1566-M-3-M 1507 PCT-4\SA\1\1> 1566-M·4·M 1508 PCT-4\SA\1\1> 1566-M-5-M 1509 PCT-4\SA\1\1> 1566-M.O·M 1510 PCT-4\SA\1\1>1576-M+M 1511 PCT·4\SA\1\1>1576·M·2·M 1512 PCT-4\SA\1\1>1576-M-3-M 1513 PCT·4\SA\1\1>1576·M·4·M 1514 PCT-4\SA\1\1:>1576-M-5-M 1515 PCT·4\SA\1\1>1576·M·6·M 1516 PCT-4\SA\1\1> 1632·M·1·M 1517 PCT-4\SA\1\1> 1632·M·2·M 1518 PCT·4\SA\1\ 1>1632·M·3·M 1519 PCT-4\SA\1\1> 1632·M·4·M 1520 PCT·4\SA\1\1>1632·M·5·M 1521 PCT·4\SA\1\1>1632· M·6·M Vg Bl Bl Fl LSc BS GO NBI 7 7 7 7 7 5 5 5 5 S 5 5 3 2 7 2 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 2 7 2 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 3 5 3 S OClE. 65 64 66 66 64 68 63 63 66 66 64 87 90 72 72 72 70 68 68 68 75 68 66 69 73 72 72 72 67 66 66 69 73 66 63 72 69 65 69 68 66 72 62 69 68 69 66 66 65 66 73 66 65 66 65 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. Fleld Nbr. 1999A Origin 19988 Pedigree Vg 81 81 Fl lSC BS GD N81 361 362 363 364 365 366 ~ 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 5940396 8940397 5940398 5940399 5940400 8940401 5940402 5940403 5940404 5940405 5940406 5940407 T1 T2 T3 5940408 5940409 8940410 5940411 5940412 5940413 5940414 5940415 5940416 5940417 5940418 8940419 5940420 5940421 5940422 8940423 5940424 8940425 1522 PCT-4\SA\1\1>1689-M-1-M 1523 PCT-4\SA\1\1>1689-M-2-M 1524 PCT-4\SA\1\1>1689-M-3-M 1525 PCT-4\SA\1\1>1689-M-4-M 1526 PCT-4\SA\1\1>1689-M-5-M 1527 PCT-4\SA\1\1>1689-M-6-M 1528 PCT-4\SA\1\1> 1702-M-1-M 1529 PCT-4\SA\1\1>1702-M-2-M 1530 PCT-4\SA\1\1>1702-M-3-M 1531 PCT-4\SA\1\1>1702-M-4-M 1532 PCT-4\SA\1\1>1702-M-5-M 1533 PCT-4\SA\1\1>1702-M-6-M CIRA0409 O.Sabana6 O .Sabana 10 1534 PCT-4\SA\1 \1>1837-M-1-M 1535 PCT-4\SA\1\1>1837-M-2-M 1536 PCT-4\SA\1\1>1837-M-3-M 1537 PCT-4\SA\1\1>1 837-M-4-M 1538 PCT-4\SA\1\1>1 837-M-5-M 1539 PCT-4\SA\1\1>1837-M-6-M 1540 PCT-4\SA\1\1>1878-M-1-M 1541 PCT-4\SA\1\1>1878-M-2-M 1542 PCT-4\SA\1\1>1878-M-3-M 1543 PCT-4\SA\1\1>1878-M-4-M 1544 PCT-4\SA\1\1>1878-M-5-M 1545 PCT-4\SA\1\1>1878-M-6-M 1546 PCT-4\SA\1\1>1928-M-1-M 1547 PCT-4\SA\1\1>1928-M-2-M 1548 PCT-4\SA\1\1>1928-M-3-M 1549 PCT-4\SA\1\1>1928-M-4-M 1550 PCT-4\SA\1\1>1928-M-5-M 1551 PCT-4\SA\1\1>1928-M-6-M 3 3 3 3 5 3 3 2 5 5 3 3 3 5 3 3 5 2 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 Vg • vigor; Bl 1 • leal blast; Bl 2 • leaf b las~ Fl • flowering; LaC • leaf scald; BS • brown apo1: NBI • neck blaa1; Gd • graln dlocoloratlon. Table 9. Selected 54 lines from the population PCT -4 \ SA \1 \1> La libertad experimental station, 1999A 50% 66 66 67 67 66 67 66 67 66 66 68 66 64 87 87 67 68 63 65 68 64 66 70 66 66 66 66 66 66 66 67 67 66 3 3 3 Nbr. Fleld Nbr. 1999A Origin 19988 Pedlgree Vg 81 81 Fl LSc 88 GD NBI 2 3 4 5 6 7 8 9 10 11 12 5940091 8940144 8940146 5940161 5940164 8940167 8940173 8940176 8940179 8 940182 5940208 8940211 1217 PCT-4\SA\1\1>195-M-2-M 1270 PCT-4\SA\1\1>341-M-1-M 1272 PCT-4\SA\1\1>341-M-3-M 1287 PCT-4\SA\1\1>446-M-6-M 1290 PCT-4\SA\1\1>500-M-3-M 1293 PCT-4\SA\1\ 1>500-M-6-M 1299 PCT-4\SA\1\1>503-M-6-M 1302 PCT-4\SA\1 \1>516-M-3-M 1305 PCT-4\SA\1\1>516-M-6-M 1308 PCT-4\SA\1\1>540-M-3-M 1334 PCT-4\SA\1\1>669-M-5-M 1337 PCT-4\SA\1 \1>721-M-2-M 5 5 5 7 7 5 5 7 5 5 7 7 50% n 61 67 66 66 64 67 67 68 65 73 70 3 3 3 3 3 3 Nbr. Fleld Nbr. Origln Pedlgree Vg 81 81 A LSc: BS GD N81 1999A 19988 5()721·M·4·M 7 69 14 5940215 1341 PCT·4\SA\1\1>721·M-6·M 7 72 15 5940218 134.4 PCT-4\SA\1\h 722-M-3-M 7 frT 16 5940223 1349 PCT-4\SA\1\1>763-M-2-M 7 63 17 5940228 1354 PCT·4\'>A\1\1>813-M·1 ·M 7 63 18 5940233 1359 PCT-4\'>A\1\1>813-M-6-M 7 frT 19 5940259 1385 PCT-4\'>A\1\1>975-M-2-M 5 66 20 5940260 1386 PCT · 4\SA\ 1\ 1 >975-M-3-M 7 69 21 5940266 1392 PCT·4\'>A\1\1>982·M-3·M 5 2 66 22 5940278 1404 PCT-4\SA\1\1>1036-M-3-M 5 73 23 5940281 1407 PCT·4\'>A\1\1>1036-M-6·M 5 72 24 5940284 1410 PCT-4\SA\1\1>1044-M-3-M 5 73 25 5940307 1433 PCT-4\'>A\1\1> 1156-M·2·M 5 63 26 5940313 1439 PCT-4\SA\1\1>1199-M-2-M 5 62 27 5940316 1442 PCT·4\'>A\1\1>1199-M·5·M 7 63 28 5940319 1445 PCT-4\'>A\1\1>1206-M-2-M 5 62 29 5940324 1450 PCT-4\'>A\1\1> 1236-M·1·M 5 62 30 5940327 1453 PCT-4\'>A\ 1\ 1 > 1236-M-4·M 5 62 31 5940331 1456 PCT-4\'>A\1\1>1241-M-1-M 7 66 32 5940334 1460 PCT·4\SA\1\1>1241·M·5·M 5 2 63 33 5940341 1467 PCT ·4\'>A\ 1\ 1 > 1260-M-6·M 7 63 34 5940343 1469 PCT·4\'>A\1\1> 1270.M·2·M 7 65 3 35 5940346 1472 PCT·4\SA\1\1>1270.M·5-M 7 66 36 5940353 1479 PCT·4\SA\1\1>1272·M-6·M 5 66 37 5940360 1486 PCT·4\'>A\1\1>1479-M·1·M 5 68 38 5940370 1496 PCT-4\'>A\ 1\ 1 > 1486-M·5·M 5 66 39 5940371 1497 PCT ·4\'>A\ 1\ 1 > 1486-M-6·M 5 66 40 5940372 1498 PCT -4\'>A\1\1> 1549-M·1· M 5 69 41 5940383 1509 PCT-4\'>A\1\1>1566-M-6-M 5 62 42 5940387 1513 PCT-4\'>A\1\1> 1576-M·4·M 5 66 3 43 5940389 1515 PCT-4\'>A\1\1> 1576-M-6·M 5 65 3 44 5940409 1535 PCT-4\SA\1\1>1837·M·2·M 5 68 Table 10. Evaluation of 5 6 lines from the population PCT -4\PHB\1 \ 1> La libertad experimental station, 1999A Nbr. Aeld Nbr. 1999A Ortgln 19938 Pedlgree Vg Bl Bl Fl LSC BS 00 NBI 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 S960001 S960002 S960003 5960004 5960005 5960006 5960007 5960008 S960009 T1 T2 T3 5960010 5960011 5960012 5960013 5960014 S960015 5960016 5960017 S960018 S960019 5960020 5960021 S960022 S960023 S960024 S960025 S960026 S960027 S960028 S960029 S960030 S960031 S960032 S960033 S960034 S960035 S960036 S960037 S960038 S960039 S960040 S960041 S960042 S960043 S960044 S960045 S960046 S960047 S960048 328 329 330 PCT-4\PHB\1\1>145-M-3-M·l·M PCT·4\PHB\1\1>145-M·3-M·2·M PCT ·4\PHB\ 1\1 > 145-M-3-M·3·M 331 PCT-4\PHB\1\1> 145-M-3-M·4·M 332 PCT·4\PHB\1\1>145-M-3-M·5·M 333 PCT-4\PHB\1\1>145-M-3-M-6-M 334 PCT-4\PHB\1\1>145-M-5-M+M 335 PCT·4\PHB\ 1\1>145-M·5-M·2·M 336 PCT ·4\PHB\ 1\ 1> 145-M-5-M-3-M CIRAD409 O.Sabana6 O.Sabana 10 337 PCT-4\PHB\ 1\1>145-M-5-M-4-M 338 PCT·4\PHB\1\1>145-M-5-M·5·M 339 PCT ·4\PHB\ 1\ 1> 145-M·5-M-6·M 346 PCT-4\PHB\ 1\ 1>209-M-6-M·l· M 347 PCT·4\PHB\1\1>209-M-6-M·2·M 348 PCT·4\PHB\1\1>209-M-6-M·3·M 349 PCT·4\PHB\1\1>209-M-6-M·4·M 350 PCT·4\PHB\1\1>209-M-6-M·5·M 351 PCT·4\PHB\1\1>209-M-6-M·6·M 353 PCT·4\PHB\1\1>231·M-6·M·1·M 359 PCT·4\PHB\1\1>231-M-6-M·2·M 360 PCT-4\PHB\1\1>231-M-6-M-3-M 361 PCT·4\PHB\1\1>231·M-6·M·4·M 362 PCT·4\PHB\1\1>231-M-6-M·5·M 363 PCT·4\PHB\1\1>231· M-6·M-6·M 364 PCT-4\PHB\1\1>277-M-3-M-1-M 365 PCT·4\PHB\1\1>277·M·3·M·2·M 366 PCT·4\PHB\1\1>277·M-3· M·3·M 367 PCT·4\PHB\1\1>277· M·3-M·4·M 368 PCT-4\PHB\f\ 1>277-M-3-M-5-M 369 PCT ·4\PHB\ 1\1 >277 ·M-3-M-6-M 376 PCT·4\PHB\1\1>277·M·5-M+ M 377 PCT·4\PHB\1\1>277·M·5-M·2·M 378 PCT·4\PHB\1\1>277·M·5-M·3·M 379 PCT·4\PHB\1\1>277·M·5-M·4·M 380 PCT·4\PHB\1\1>277·M·5-M·5·M 381 PCT·4\PHB\1\1>277·M·5-M-6·M 382 PCT ·4\PHB\ 1\ 1>277 ·M-6-M+M 383 PCT-4\PHB\1\1>277·M-6-M·2·M 384 PCT·4\PHB\1\ 1>277·M-6·M·3·M 365 PCT·4\PHB\1\1>277· M·6·M·4·M 386 PCT·4\PHB\1\1>277-M-6-M·5·M 387 PCT·4\PHB\1\1>277·M-6-M-6·M 388 PCT-4\PHB\1\1>368-M+ M+M 389 PCT·4\PHB\1\1>368·M-1-M·2·M 390 PCT·4\PH8\1\1>368· M· 1· M·3·M 391 PCT ·4\PHB\ 1\ 1>368-M· 1 ·M·4·M 392 PCT·4\PHB\1\1>368· M· 1· M·5·M 393 PCT·4\PHB\1\1>368·M+M·6·M 7 3 5 3 3 3 5 2 5 3 5 2 7 2 5 7 5 3 7 2 7 7 7 7 7 7 5 7 2 5 7 2 7 2 5 2 7 SO% 2 73 2 68 2 72 66 74 2 64 2 67 67 64 64 65 87 65 66 66 68 73 70 73 74 70 66 66 65 66 5 66 7 66 7 3 85 5 80 5 2 2 83 5 2 80 7 2 83 7 2 2 79 7 69 7 2 72 7 73 5 5 7 7 2 7 7 7 7 7 5 5 5 7 5 5 74 75 68 65 66 63 66 66 63 66 61 67 64 63 63 3 3 3 3 3 3 3 3 3 3 3 5 3 3 3 5 3 3 3 3 3 3 Nbr. ~ 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 n 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 Aeld Nbr. 18119A 8960049 5960050 5960051 S9S0052 S9S0053 ~ 5960055 5960056 5960057 5960058 5960059 5960060 5960061 5960062 5960063 5960064 5960065 5960066 T1 T2 T3 T1 T2 T3 5960067 5960068 5960069 5960070 5960071 5960072 5960073 5960074 5960075 5960076 59600n 5960078 5960079 5960080 5960081 5960082 5960083 5960084 5960085 S960086 5960087 5960088 5960089 5960090 5960091 5960092 5960093 5960094 5960095 5960096 5960097 Origln 19988 Pttdlgree 406 PCT-4\PHB\1\1>453-M-1-M-1-M 407 PCT-4\PHB\1\1>453-M-1-M-2-M 408 PCT-4\PHB\1\1>453-M-1-M-3-M 409 PCT-4\PHB\1\1>453-M-1-M-4-M 410 PCT-4\PHB\1\1>453-M-1-M-5-M 411 PCT-4\PHB\1\1>453-M-1-M-6-M 412 PCT-4\PHB\1\1>453-M-2-M-1-M 413 PCT-4\PHB\1\1>453-M-2-M-2-M 414 PCT-4\PHB\1\1>453-M-2-M-3-M 415 PCT-4\PHB\1\1>453-M-2-M-4-M 416 PCT-4\PHB\1\1>453-M-2-M-5-M 417 PCT-4\PHB\1\1>453-M-2-M-e-M 418 PCT-4\PHB\1\1>485-M-2-M-1-M 419 PCT-4\PHB\1\1>435-M-2-M-2-M 420 PCT -4\PHB\ 1\ 1>485-M-2-M-3-M 421 PCT-4\PHB\1\1>485-M-2-M-4-M 422 PCT-4\PHB\1\1>485-M-2-M-5-M 423 PCT-4\PHB\1\1>485-M-2-M-e-M CIRAD409 O.Sabana6 O.Sabana 10 CIRA0409 O.Sabana6 O.Sabana 10 424 PCT-4\PHB\1\1>538-M-2-M-1-M 425 PCT-4\PHB\1\1>538-M-2-M-2-M 426 PCT-4\PHB\1\1>538-M-2·M·3·M 427 PCT-4\PHB\1\1>538-M-2-M-4-M 428 PCT-4\PHB\1\1>538-M-2-M-5-M 429 PCT -4\PHB\ 1\1 >538-M-2-M--e-M 436 PCT-4\PHB\1\1>603-M-5-M-1-M 437 PCT-4\PHB\1\1>603-M-5-M-2-M 438 PCT -4\PHB\ 1\1 >603-M-5-M-3-M 439 PCT-4\PHB\1\1>603-M-5-M-4-M 440 PCT-4\PHB\1\1>603-M-5-M-5-M 441 PCT-4\PHB\1\1>603-M-5-M-e-M 442 PCT-4\PHB\1\1>749-M-2-M-1-M 443 PCT-4\PHB\ 1\1>749-M-2-M-2-M 444 PCT-4\PHB\1\1>749-M-2-M-3-M 445 PCT-4\PHB\1\1>749-M-2-M-4-M 446 PCT-4\PHB\1\1>749-M-2-M-5-M 447 PCT-4\PHB\1\1>749-M-2-M-e-M 448 PCT-4\PHB\1\1>751-M-3-M-1-M ~ PCT-4\PHB\1\1>751-M-3-M-2-M 450 PCT-4\PHB\1\1>751-M-3-M-3-M 451 PCT-4\PHB\1\1>751-M-3-M-4-M 452 PCT-4\PHB\1\ 1>751-M-3-M-5-M 453 PCT-4\PHB\1\1>751-M-3-M-6-M 460 PCT-4\PHB\1\1>752-M-1-M-1-M 461 PCT-4\PHB\1\1>752-M-1-M-2-M 462 PCT-4\PHB\ 1\1>752-M-1-M-3-M 463 PCT-4\PHB\1\ 1>752-M-1-M-4-M 464 PCT-4\PHB\1\1>752-M-1-M-5-M 465 PCT-4\PHB\1\1>752-M-1-M-e-M 472 PCT-4\PHB\1\1>752-M-4-M-1-M Vg Bl Bl A LSc BS GD NBI 5 5 2 5 1 5 2 5 5 7 5 5 5 5 7 7 2 7 7 5 7 7 3 7 5 2 5 5 5 7 5 5 5 5 7 5 5 7 7 5 5 5 5 5 7 7 7 4 7 3 7 2 5 2 7 7 5 7 5 5 7 5 5 5 5 50'!{, 68 69 69 72 71 69 68 68 66 63 75 68 2 76 75 69 70 73 78 66 86 88 63 86 89 68 66 66 68 69 72 73 75 68 64 73 63 66 74 71 70 4 70 3 69 2 69 2 66 66 72 70 90 3 78 66 3 64 63 64 62 82 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 Fleld Nbr. 199SIA 8960098 8960099 5960100 5960101 5960102 5960103 5960104 5960105 5960106 5960107 5960108 5960109 5960110 5960111 5960112 5960113 5960114 5960115 5960116 5960117 5960118 5960119 5960120 5960121 5960122 5960123 T1 T2 T3 5960124 5960125 5960126 5960127 5960128 5960129 5960130 5960131 5960132 5960133 5960134 5960135 5960136 5960137 5960138 5960139 5960140 5960141 5960142 5960143 5960144 5960145 5960146 5960147 5960148 Origln 19988 Pedlgree 473 PCT-4\PHB\1\1>752-M-4-M-2-M 474 PCT-4\PHB\1\1>752-M-4-M-3-M 475 PCT-4\PHB\1\1>752-M-4-M-4-M 476 PCT-4\PHB\1\1>752-M-4-M-5-M 477 PCT-4\PHB\1\1>752-M-4-M-6-M 484 PCT-4\PHB\1\1>783-M-4-M-1-M 485 PCT-4\PHB\1\1>783-M-4-M-2-M 486 PCT-4\PHB\1\1>783-M-4-M-3-M 487 PCT ·4\PHB\ 1\1 > 783-M-4-M-4-M 488 PCT-4\PHB\1\1>783-M-4-M-5-M 489 PCT-4\PHB\1\ 1>783-M-4-M-6-M 490 PCT-4\PHB\1\1>783-M-5-M-1-M 491 PCT-4\PHB\1\1>783-M-5-M-2-M 492 PCT-4\PHB\1\1>783-M-5-M-3-M 493 PCT-4\PHB\1\1>783-M-5-M-4-M 494 PCT -4\PHB\ 1\1 > 783-M-5-M-5-M 495 PCT -4\PHB\ 1\1 > 783-M-5-M-6-M 496 PCT-4\PHB\1\1>822-M-1-M-1-M 497 PCT·4\PHB\1\1>822·M-1-M·2-M 498 PCT-4\PHB\1\ 1>822-M-1-M-3-M 499 PCT-4\PHB\1\1>822·M-1·M·4·M 500 PCT-4\PHB\1\1>822-M-1-M-5-M 501 PCT-4\PHB\1\1>822-M-1-M-6-M 508 PCT-4\PHB\1\1>846-M-4-M-1-M 509 PCT-4\PHB\1\1>846-M-4-M-2-M 510 PCT-4\PHB\1\1>846-M-4-M-3-M CIRA0409 O.Sabana6 O.Sabana 10 511 PCT-4\PHB\ 1\1>846-M-4-M-4-M 512 PCT-4\PHB\1\1>846-M-4-M-5-M 513 PCT-4\PHB\1\1>846-M-4-M-6-M 514 PCT-4\PHB\1\1>1678-M-4-M-1-M 515 PCT-4\PHB\1\1>1678-M-4-M-2-M 516 PCT-4\PHB\1\1>1678-M-4-M-3-M 517 PCT-4\PHB\1\1> 1678-M-4-M-4-M 518 PCT-4\PHB\1\1> 1678-M-4-M-5-M 519 PCT-4\PHB\1\1>1678-M-4-M-6-M 520 PCT-4\PHB\1\1>1723-M-1-M-1-M 521 PCT -4\PHB\1\1>1723-M-1-M-2-M 522 PCT·4\PHB\1\1>1723·M·1· M-3-M 523 PCT-4\PHB\1\1>1723-M-1-M-4-M 524 PCT-4\PHB\1\1> 1723-M-1-M-5-M 525 PCT-4\PHB\1\1>1723-M-t-M-6-M 526 PCT-4\PHB\1\1>1723-M·2·M-1·M 527 PCT-4\PHB\1\1>1723-M-2-M-2-M 528 PCT ·4\PHB\ 1\ 1> 1723-M-2-M-3-M 529 PCT·4\PHB\1\1>1723·M·2·M-4-M 530 PCT-4\PHB\1\1>1723-M-2-M-5-M 531 PCT-4\PHB\1\1>1723-M-2-M-6-M 532 PCT-4\PHB\1\1> 1723-M·3-M-1·M 533 PCT-4\PHB\1\1>1723-M-3-M-2-M 534 PCT-4\PHB\1\1>1723-M-3-M-3-M 535 PCT-4\PHB\1\1>1723-M-3-M-4-M Vg Bl Bl A LSc BS GD NBI 7 7 3 7 3 5 2 5 2 5 3 5 2 7 2 7 3 5 2 7 5 3 5 2 5 3 5 3 5 3 7 2 5 5 5 5 5 5 5 5 3 3 3 3 3 2 3 2 5 5 3 2 3 2 3 3 3 2 5 3 5 4 5 2 5 3 5 2 5 3 5 2 5 3 3 5 3 3 3 3 3 50'!1. 77 83 1 84 2 76 80 76 80 3 84 83 82 82 68 69 3 64 2 66 66 3 74 72 69 69 69 68 70 66 70 66 66 2 85 87 69 2 68 63 63 64 64 66 74 71 82 3 79 3 84 83 83 2 85 2 87 73 66 66 83 81 70 71 69 75 3 3 3 3 3 3 1 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 5 3 3 3 3 3 5 3 3 Nbr. Fleld Nbr. Ortgln Pedlg.-.. Vg 81 81 Fl LSC BS GD N81 1999A 19988 50% 149 8960149 536 PCT -4\PHB\ 1\ 1> 1723-M-3-M·S...M 5 2 2 75 150 8960150 537 PCT -4\PHB\ 1\ 1> 1723-M-3-M-6-M 5 3 86 3 Vg • vigor, 811 •IMI blut; 812 •IMI biMt; Fl• flowenng; LIC • 1Mt acald; 8S • brown apo1; N81 • Mek biMt; Gd • IJ La Libertad experimento.( statioo, 1999A Nbr. Fleld Nbr. Ortgln Pedlg.-.. Vg 81 81 Fl LSC BS GD N81 1999A 19988 50% 5960004 331 PCT-4\PHB\1\1>145-M-3-M-4-M 5 2 66 3 2 S96001Z 339 PCT-4\PHB\1\1>145-M-5-M-6-M 7 66 3 8960021 360 PCT-4\PHB\1\1>231-M-6-M-3-M 5 2 65 4 8960051 408 PCT-4\PHB\1\1>453-M-1-M-3-M 5 69 5 S960068 425 PCT-4\PHB\1\1>538-M-2-M-2-M 5 66 6 S960069 426 PCT-4\PHB\1\1>538-M-2-M-3-M 5 66 7 8960072 429 PCT -4\PHB\1\ 1 >538-M-2-M-6-M 5 72 8 8960077 440 PCT-4\PHB\1\1>603-M-5-M-5-M 5 73 9 S960116 497 PCT ·4\PHB\ 1\ 1>822-M-1-M-2·M 5 69 10 S960123 510 PCT -4\PHB\ 1\ 1>846-M-4-M-3-M 3 66 11 S960124 511 PCT -4\PHB\ 1\ 1>845-M-4-M-4-M 3 2 69 12 S960127 514 PCT-4\PHB\1\1>167&-M-4-M-1-M 3 2 63 13 5960132 519 PCT -4\PHB\ 1\1> 167&-M-4-M-6-M 5 71 3 Table 11. Evaluation of 58 lin~ from the populations PCT-5\0\0\0>, PCT-A \ 0 \ 0\0> y PCT -4\0\0\1> La libertad experimental station, 1999A Nbr. 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 Fleld Nbr. Orlgln Pedlgree 1999A 19988 5980001 5980002 5980003 5980004 5980005 5980006 5980007 5980008 5980009 5980010 5980011 5980012 5980013 5980014 5980015 5980016 5980017 5980018 5980019 5980020 5980021 5980022 5980023 5980024 $980025 $980026 $980027 T1 T2 T3 $980028 $980029 5980030 5980031 $980032 $980033 $980034 5980035 $980036 $980037 $980038 $980039 5980040 5980041 5980042 5980043 5980044 5980045 $980046 5980047 5980048 638 PCT -5\0ID\0> 1496-M-1-M-3-M-1-M 539 PCT ·5\0ID'D> 1496-M-1-M-3-M-2-M 540 PCT ·5\0ID\0> 1496-M-1-M-3-M-3-M 541 PCT -5\0ID\0> 1496-M-1-M-3-M-4-M 542 PCT -5\0ID\0> 1496-M-1-M-3-M-5-M 543 PCT ·5\0ID'D> 1496-M-1-M-3-M~-M 544 PCT·AIDID\0>175-M-1·M·4·M-1-M 545 PCT·AIDID\0>175-M-1-M-4·M·2-M 546 PCT·AIDID\0> 175-M-1·M-4·M·3-M 547 PCT·AIDID'D>175-M·1·M·4·M-4-M 548 PCT·AIDID\0>175-M-1·M+M·5-M 549 PCT ·A IDID\0> 175-M-1·M·4·M·6-M 550 PCT·A\OIDID>175-M-1-M·5-M·1-M· 551 PCT·AIDID\0>175-M-1-M·5-M·2·M 552 PCT ·A IDIDID> 175-M· 1·M·5·M·3-M 553 PCT·A IDIDID> 175-M-1·M·5-M·4-M 554 PCT·AID\O'D> 175-M·1·M·5·M·5-M 555 PCT·AIDIDID> 175-M·1·M·5-M·6-M 556 PCT·AIDID\0>175-M-3-M-1-M-1-M 557 PCT·AIDID\0>175-M-3-M-1-M·2·M 558 PCT·AIDID\0>175-M·3-M·1 ·M·3-M 559 PCT·AIDIDID> 175-M·3-M·1·M·4-M 560 PCT ·A ID\0\0> 175-M·3·M-1-M·5-M 561 PCT·AIDID\0>175-M-3-M-1-M-6-M 562 PCT·AIDID\0> 175-M·3-M·3·M·1·M 563 PCT ·A IDID'D> 175-M·3-M-3-M·2·M 564 PCT·AIDID'D> 175-M-3-M-3-M-3-M CIRAD409 O .Sabana6 0 .5abana 10 565 PCT·AIDIDID>175-M·3-M·3-M-4-M 566 PCT-A\0\0ID> 175-M-3-M-3-M·s-M 567 PCT·AIDID\0> 175-M-3-M-3-M-6-M 574 PCT·AIDID\0>175-M-4·M·4·M·1·M 575 PCT·AIDID'D>175-M·4-M·4·M·2·M 576 PCT ·A IDIDID> 175-M·4-M·4·M·3-M 577 PCT·AIDID'D> 175-M·4· M·4·M·4·M 578 PCT ·A IDID'D> 175-M-4-M·4·M·5-M 579 PCT·AIDID\0> 175-M-4-M-4-M-6-M 580 PCT ·A IDVJID> 175-M-4-M-5-M-1-M 581 PCT·AIDID\0>175-M·4-M·5-M·2·M 582 PCT ·A VJVJ\0> 175-M-4-M·5·M·3-M 583 PCT ·AVJVJ\0> 175·M·4·M·S·M-4-M 584 PCT·AVJ\OVJ>175-M-4-M·5·M·5-M 585 PCT·A\0\0\0>175-M·4· M·5-M·6-M 586 PCT·A\0\0'D> 175-M·G-M-1-M-1-M 587 PCT ·A VJVJVJ> 175-M·6-M· 1·M·2·M 588 PCT·AVJVJ'D>175-M·6-M·1·M·3·M 589 PCT·A\0\0\0>175-M·6-M-1-M· 4-M 590 PCT·A\OVJID>175-M-6-M· 1·M·5-M 591 PCT·A\0\0\0>175·M·6·M-1-M·6-M Vg Bl Bl Fl LSc BS 00 NBI 3 2 3 3 3 2 3 2 3 4 3 5 4 2 5 4 3 7 4 3 7 4 3 7 3 7 3 7 4 4 5 3 3 5 3 3 7 3 7 2 7 2 5 3 2 5 2 3 5 3 5 3 2 5 2 50% 76 75 78 75 75 75 63 66 63 63 64 62 63 62 64 66 63 63 63 62 63 62 62 3 63 5 3 2 69 7 2 70 7 2 69 5 66 5 4 2 86 7 2 89 5 2 68 7 3 69 7 4 2 72 7 2 69 7 2 69 7 3 2 66 7 2 66 7 2 2 72 7 69 5 68 7 70 5 66 5 2 69 5 72 7 66 7 7 7 2 7 7 7 69 70 73 75 75 75 3 3 3 3 3 3 3 3 3 3 Nbr. 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 Aeld Nbr. Orlgln Pedlgree 1999A 5980049 8980050 89&0051 5980052 89&0053 5980054 T1 T2 T3 5980055 5980056 89&0057 5980058 89&0059 5980060 5980061 5980062 5980063 5980064 5980065 59&0066 5980067 5980068 5980069 5980070 5980071 5980072 5980073 5980074 5980075 5980076 5980077 89&0078 5980079 59800&0 5980081 5980082 5980083 5980084 5980085 5980086 5980087 5980088 5980089 5980090 5980091 5980092 5980093 5980094 5980095 5980096 5980097 5980098 5980099 5980100 19988 592 PCT·A\0\0\0>189-M-2·M-2·M+M 593 PCT·A\0\0\0>1~M-2·M·2·M-2-M 594 PCT·A\0\0\0>189-M·2·M·2·M·3-M 595 PCT·A\0\0\0>189-M-2·M·2·M-4-M 596 PCT-A\0\0\0>1~M-2·M·2·M·5-M 597 PCT·A\0\0\0>1~M-2·M-2·M·6-M CIRAD409 O .Sabana6 O.Sabana 10 598 PCT·A\0\0\0>189-M-4-M-3-M-1-M 599 PCT·A\0\0\0>1~M-4-M-3-M·2·M 600 PCT·A\0\0\0>1~M-4-M-3-M-3-M 601 PCT·A\0\0\0> 189-M-4-M-3-M-4-M 602 PCT·A\0\0\0>189-M-4-M-3-M-5-M 603 PCT·A\0\0\0> 189-M-4-M-3-M-6-M 604 PCT·A\0\0\0>1~M-4-M-4-M-1·M 605 PCT·A\0\0\0>1~M-4-M-4·M-2-M 606 PCT·A\0\0\0>189-M·4-M·4·M·3-M 607 PCT ·A \0\0\0> 189-M-4-M-4-M-4-M 608 PCT·A\0\0\0>189-M-4-M-4·M·5-M 609 PCT ·A \0\0\0> 189-M·4·M·4·M·6-M 610 PCT·A\0\0\0>189-M-4-M·5-M·1·M 611 PCT·A\0\0\0> 1~M-4·M·5-M·2·M 612 PCT ·A \0\0\0> 189-M-4-M·S.M-3-M 613 PCT ·A \0\0\0> 189-M·4·M·5·M·4·M 614 PCT ·A \0\0\0> 189-M·4· M·5-M·5-M 615 PCT ·A \0\0\0> 189-M-4-M-5-M-6-M 616 PCT·A \0\0\0> 1 ~M-4-M-6-M-1-M 617 PCT·A\0\0\0>189-M·4·M-6-M·2-M 618 PCT·A\0\0\0>189-M-4-M-6-M-3-M 619 PCT ·A \0\0\0> 189-M-4-M-6-M-4-M 620 PCT·A\0\0\0>189-M-4-M-&·M·S.M 621 PCT·A\0\0\0>189-M·4·M-6-M·6-M 622 PCT·A\0\0\0>278-M-1·M+M·1-M 623 PCT·A\0\0\0>278-M-1·M·1·M·2·M 624 PCT ·A \0\0\0>278·M·1·M+M·3-M 625 PCT·A\0\0\0>278-M·1·M+M·4-M 626 PCT·A\0\0\0>278-M·1·M+M·5-M 627 PCT·A\0\0\0>278-M·1·M+M·6-M 628 PCT ·A \0\0\0>278·M·1·M·2·M·1·M 629 PCT·A\0\0\0>278-M·1·M·2· M·2·M 630 PCT·A\0\0\0>278-M-1 ·M-2·M·3-M 631 PCT·A\0\0\0>278·M·1·M·2·M·4-M 632 PCT-A\0\0\0>278·M·1·M·2· M·5-M 633 PCT ·A \0\0\0>278·M·1·M·2· M-6-M 634 PCT·A\0\0\0>394-M-1-M-1-M-1-M 635 PCT-A\0\0\0>394-M-1 -M-1-M-2-M 636 PCT ·A \0\0\0>394·M· 1·M-1· M-3-M 637 PCT ·A \0\0\0>394·M·1·M·1· M-4-M 638 PCT ·A \0\0\0>394-M· 1· M·1·M-5-M 639 PCT·A\0\0\0>394-M·1· M·1· M-6·M 640 PCT-A\0\0\0>394-M-1·M-4·M-1-M 641 PCT·A\0\0\0>394·M·1·M·4· M-2·M 642 PCT ·A \O\OID>394·M· 1·M·4·M-3-M 643 PCT ·A \0\0\0>394-M·1· M-4· M-4·M Vg 81 81 Fl LSc 85 GO N81 7 7 7 7 7 2 2 7 2 2 7 5 4 4 7 2 2 5 4 4 5 5 5 5 5 5 5 5 5 5 4 4 5 3 3 5 4 4 5 2 2 5 2 2 5 3 3 5 2 2 5 3 3 5 3 3 5 3 3 5 3 3 5 3 3 5 3 3 5 2 2 7 3 3 5 2 2 7 3 3 5 3 3 5 3 3 5 3 3 7 4 4 5 3 3 5 3 3 7 2 2 5 3 3 5 3 3 3 4 4 3 3 3 5 4 4 7 4 4 3 4 4 5 3 3 5 2 2 5 2 2 5 5 5 4 4 7 3 3 3 3 3 5 2 5 2 3 5 2 50% 83 3 83 3 84 3 84 3 85 3 84 3 66 86 3 89 3 83 3 3 3 82 3 3 3 82 3 3 3 80 3 3 80 3 3 3 80 3 3 3 80 80 80 82 82 82 82 82 82 82 82 82 81 81 81 81 82 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 82 3 82 3 82 3 3 81 3 3 83 3 3 82 3 3 81 3 3 80 3 3 80 3 80 3 83 3 82 3 3 80 3 85 84 85 84 82 85 74 74 75 75 3 3 3 3 2 2 2 2 Nbr. 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 Aeld Nbr. Origin Pedlgree 1999A 19988 5980101 644 PCT -A \0\0\0>394-M-1-M-4-M-5-M 5980102 5980103 5980104 5980105 5980106 5980107 5980108 S980109 S980110 S980111 S980112 S980113 S980114 T1 T2 T3 $980115 S980116 S980117 S980118 5980119 $980120 5980121 5980122 S980123 S980124 S9801 25 8980126 S980127 S980128 S980129 S980130 S980131 S980132 $980133 S980134 S980135 S980136 5980137 $980138 $980139 $980140 S980141 S980142 S980143 $980144 S980145 S980146 S980147 S980148 S980149 S980150 S9801 51 5 980152 645 PCT -A \0\0\0>394-M-1-M-4-M-6-M 646 PCT ·A \0\0\0>394-M-1·M·5-M·1·M 647 PCT-A\0\0\0>394-M-1-M-5-M-2-M 648 PCT -A \0\0\0>394-M-1-M·5-M·3-M 649 PCT·A\0\0\0>394-M-1·M·5-M·4-M 650 PCT ·A \0\0\0>394·M·1·M·5-M·5-M 651 PCT ·A \0\0\0>394·M-1-M·5-M·6-M 652 PCT ·A \0\0\0>394-M-1-M-6-M·1-M 653 PCT·A\0\0\0>394·M-1-M-6-M·2·M 654 PCT -A \0\0\0>394·M-1·M-6-M·3-M 655 PCT ·A \0\0\0>394·M+M-6-M·4-M 656 PCT·A\0\0\0>394-M-1-M-6-M-5-M 657 PCT ·A \0\0\0>394·M·1·M-6-M·6-M CIRA0409 O .Sabana6 O.Sabana 10 658 PCT·A\0\0\0>394·M· 2·M+M+M 659 PCT·A\0\0\0>394·M·2·M·1·M·2-M 660 PCT·A\0\0\0>394·M·2·M-1-M·3-M 661 PCT·A\0\0\0>394·M·2·M-1-M-4-M 662 PCT·A \0\0\0>394·M·2·M·1·M·5-M 663 PCT·A\0\0\0>394·M·2·M+M·6-M 664 PCT·A\0\0\0>394·M·2-M-2·M-1-M 665 PCT·A\0\0\0>394·M·2· M·2·M-2-M 666 PCT·A\0\0\0>394·M·2·M·2· M-3-M 667 PCT·A\0\0\0>394·M·2·M·2· M·4-M 668 PCT·A\0\0\0>394·M-2-M·2· M·5-M 669 PCT·A\0\0\0>394-M·2·M-2·M·6-M 670 PCT·A\0\0\0>394·M-2·M·3·M-1-M 671 PCT·A\0\0\0>394·M·2·M-3·M·2·M 672 PCT·A\0\0\0>394·M·2·M·3-M·3-M 673 PCT·A\0\0\0>394·M-2·M·3·M·4-M 67 4 PCT ·A \0\0\0>394·M-2·M·3·M·5-M 675 PCT·A\0\0\0>394·M·2·M-3-M·6-M 676 PCT·A\0\0\0>503-M-1-M-t-M-1-M 677 PCT ·A \0\0\0>503·M-1-M-1-M·2·M 678 PCT-A\0\0\0>503·M+M+M·3-M 679 PCT ·A \0\0\0>503·M·1·M·1·M·4-M 680 PCT ·A \0\0\0>503-M·1·M·1·M·5-M 681 PCT·A\0\0\0>503·M-1-M·1·M·6-M 682 PCT ·A \0\0\0> 1 1 69-M-1-M·4·M+M 683 PCT·A\0\0\0> 1169-M-1-M·4·M·2·M 684 PCT ·A \0\0\0> 1169-M-1-M·4-M·3-M 685 PCT ·A \0\0\0> 1 1 69·M· 1· M·4·M·4-M 686 PCT ·A \0\0\0> 1 1 69·M+M·4·M·5-M 687 PCT ·A \O'D\0> 1169·M·1·M·4· M·6-M 694 PCT ·A \()\0\0> 1321·M·2·M·4·M-1-M 695 PCT ·A \0\0\0> 1321·M·2·M·4·M·2·M 696 PCT·A\0\0\0> 1321-M·2·M·4·M-3·M 697 PCT·A\0\0\0>1321·M·2·M·4·M·4·M 698 PCT·A\0\0\0>1321·M·2·M·4·M·5-M 699 PCT·A\0\0\0>1321·M·2-M·4·M·6·M 706 PCT ·A \0\0\0> 1485-M· 1·M·4-M·1·M 707 PCT·A\0\0\0> 1485·M+M·4·M·2·M Vg 81 Bl A LSc BS GD NBI 50% 5 2 75 5 2 3 2 3 2 3 3 2 3 2 3 2 3 2 3 2 3 2 5 2 5 2 5 2 2 5 3 2 7 5 3 2 5 3 5 2 2 5 5 5 2 7 2 7 7 7 7 7 7 5 7 2 5 2 5 5 1 5 2 2 5 2 5 5 5 5 5 5 5 5 5 5 5 5 3 3 3 3 3 3 3 5 74 84 3 79 3 78 3 80 3 82 3 83 3 78 78 78 78 n 80 66 85 89 84 80 84 76 84 84 81 81 81 81 82 82 81 82 82 80 81 80 75 n 79 74 75 71 68 68 66 66 68 66 61 63 62 63 74 62 67 74 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. 153 154 155 168 157 158 159 160 161 162 153 154 155 166 167 168 169 170 171 172 173 174 175 176 1n 178 179 160 181 182 183 1114 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 Aeld Nbr. Ortgln PedlgrM 1999A 19988 S960153 708 PCT ·A VJI/JIO> 1485-M-1-M-4-M-3-M S960154 709 PCT·AIDID\0>1485-M-1 -M-4-M-4-M 5960155 710 PCT·AIDIDVJ>1485-M-1·M-4-M-5-M S960156 711 PCT ·A VJI/JIO> 1485-M-1-M-4-M-6-M S960157 S960158 5960159 S960160 S960161 8980162 5960163 S960154 S960155 S960166 S960167 S960168 T1 T2. T3 S960169 5960170 5960171 S980172 5960173 5980174 5960175 5960176 59601n 5960178 5960179 5960160 5980181 5980182 5960183 59601114 5980185 5960186 5960187 5960188 5980189 5960190 5960191 5960192 5980193 5960194 8980195 T 1 T2 T3 5960196 5960197 5960198 S980199 5980200 5 980201 718 PCT·A \O'D'D> 1488-M-4-M-1-M-1-M 719 PCT ·A \O'D'D> 1488-M-4-M-1-M-2-M 720 PCT·A'D'D'D>14a&-M-4-M-1-M-3-M 721 PCT ·A \O'D'D> 14a&-M-4-M-1·M-4-M 722 PCT ·A VJI/JIO> 1488-M-4-M-1-M-5-M 723 PCT ·A IDIDVJ> 1488-M-4-M-1-M-6-M 724 PCT·A IDIDVJ> 1488-M-4-M-4-M-1-M 725 PCT·AIDID'D> 1488-M-4-M-4-M-2-M 726 PCT·AIDID'D>14a&-M-4-M-4-M-3-M 727 PCT ·A ID'D'D> 1488-M-4-M-4-M-4-M 728 PCT ·A IDID'D> 1488-M-4-M-4-M-5-M 729 PCT ·A \O'D'D> 1488-M-4-M-4-M-6-M CIAAD409 O.Sabana 6 O.Sabana 10 730 PCT ·A IDID'D> 1488-M-4-M-5-M-1-M 731 PCT ·A IDID'D> 14M-M·4·M·5-M·2-M 733 PCT ·A IDID'D> 1488-M-4-M-5-M-4-M 734 PCT ·A 'IJVJVJ> 1488-M-4-M-5-M-5-M 735 PCT ·A IDVJVJ> 1488-M-4-M-5-M-6-M 736 PCT ·A IDVJVJ> 1488-M-5-M-1-M-1-M 737 PCT·AIDID\0>1488-M-5-M-1-M-2-M 738 PCT·AVJID'D>14a&-M-5-M-1·M-3-M 739 PCT·A IDIDVJ> 1488-M-5-M-1-M-4-M 7 40 PCT ·A IDIDVJ> 1488-M-5-M-1-M-5-M 7 41 PCT ·A IDIDVJ> 1488-M-5-M-1-M-6-M 748 PCT-AIDID\0>1674-M-2-M-4-M-1-M 749 PCT·AIDVJVJ>1674·M·2·M·4·M·2·M 750 PCT·AIDVJVJ>1674·M·2·M·4· M·3-M 751 PCT ·A VJI/JIO> 167 4-M-2-M-4-M-4-M 752 PCT·AVJID'D>1674-M-2·M-4-M-5-M 753 PCT·AVJIDVJ>1674-M-2· M·4·M-6-M 754 PCT·A VJI/JIO> 167 4-M-6-M-3-M-1-M 755 PCT ·A VJID'D> 167 4-M-6-M-3-M-2-M 756 PCT·AVJVJ\0>1674-M-6-M-3-M-3-M 757 PCT·AIDIDVJ>1674·M·6-M-3·M·4·M 758 PCT·AIDID\0>1674-M-6-M-3-M-5-M 759 PCT ·A IDID'D> 167 4-M-6-M-3-M-&-M 760 PCT ·A IDIDVJ> 167 4-M-6-M~·M-1 -M 761 PCT ·A IDVJVJ> 167 4-M-6-M-6-M-2-M 762 PCT ·A IDIDVJ> 167 4-M-6-M-6-M-3-M 763 PCT -A VJVJVJ> 167 4· M·6-M-6-M·4·M CIRAD 409 O.Sabana6 O.Sabana 10 764 PCT·A IDVJVJ> 167 4-M-6-M-6-M-5-M 765 PCT·AIDVJID> 1674·M· 6-M-6-M·6-M 766 PCT-AIDIDVJ>1788-M-2· M·2·M-1-M 767 PCT·AIDVJVJ> 1788-M-2-M-2-M-2-M 768 PCT·AIDIDVJ> 17M·M·2·M·2·M·3-M 769 PCT ·A 'IJ'IJVJ> 1788·M·2·M·2· M·4· M Vg Bl 81 Fl LSc BS 00 NBI 50'!1. 3 86 3 3 86 3 3 86 3 3 2 67 3 5 3 3 3 3 3 3 3 3 3 3 3 3 3 3 5 2 5 5 5 5 5 5 5 5 5 5 5 5 5 7 5 5 5 5 2 5 2 3 5 2 5 3 2 5 3 5 3 3 2 5 5 3 5 3 3 5 2 3 2 3 3 3 3 5 2 5 2 5 2 S9 73 S9 73 73 66 85 82 81 as as 114 63 as 87 aa aa 89 89 aa 114 87 87 114 as 85 66 69 74 75 71 72 70 73 69 73 70 72 74 68 aa 66 66 as aa 72 75 73 75 74 72 5 3 5 3 5 3 3 3 3 3 3 5 3 5 3 5 3 5 3 3 3 5 3 5 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 Fleld Nbr. Origln Pedlgree 1999A S980202 5980203 5980204 5980205 5980206 5980207 8980208 S980209 S980210 5980211 S980212 5980213 5980214 5980215 5980216 S980217 5980218 S980219 5980220 5980221 5980222 T1 T2 T3 5980223 8980224 5980225 5980226 8980227 5980228 5980229 5980230 5980231 8980232 5980233 5980234 8980235 5980236 5980237 5980238 S980239 5980240 5980241 8980242 5980243 5980244 8980245 8980246 5980247 5980248 S980249 S980250 8980251 8980252 S980253 19988 no PCT-A'4:1'4:l'D>1788-M-2-M·2-M-5-M n1 PCT-A\D\O'D>1788-M-2-M·2-M-6-M n2 PCT-AVJVJ'D>1955-M-3-M·4·M-1·M n3 PCT-AVJVJ'D>1955-M-3-M-4-M-2-M n 4 PCT ·A \D\O'D> 1955-M-3-M-4-M-3-M n5 PCT ·A \D\O'D> 1955-M-3-M-4-M-4-M n6 PCT -A VJVJVF> 1955-M-3-M-4-M-5-M 7n PCT·A'4:1VJ'D>1955-M-3-M-4-M-6-M 790 PCT -A \D\O'D>2083-M-1·M-&-M-1-M 791 PCT-AVJVJ'D>2083-M-1-M-&-M-2-M 792 PCT-AVJVJVJ>2083-M-1-M-&-M-3-M 793 PCT -A \D\O'D>2083-M-1-M-&-M-4-M 794 PCT-A \D\O'D>2083-M-1·M-&-M-5-M 795 PCT-AVJVJVF>2083-M-1·M-&-M-6-M 796 PCT -A VJVJ'D>2083-M-2-M-1-M- 1-M 797 PCT-A\D\O'D>2083-M-2·M-1-M-2-M 798 PCT-A \D\O'D>2083-M-2-M-1-M-3-M 799 PCT-AVJVJVJ>2083-M·2·M-1-M·4-M 800 PCT·A'4:1'4:l'D>2083-M·2·M-1-M-5-M 801 PCT-A\D\O'D>2083-M-2-M-1-M-6-M 802 PCT-AVJVJ'D>2083-M-2·M-3-M-1-M CIRAD409 O.Sabana6 O.Sabana 10 803 PCT-AVJVJ'D>2083-M-2-M-3-M-2-M 804 PCT-AVJVJ'D>2083-M·2·M·3-M-3-M 805 PCT-AVJ'4l'D>2083-M-2-M-3-M-4-M 806 PCT·AVJ'4:l'D>2083-M-2·M-3-M-5-M 807 PCT -A VJVJVF>2083-M·2·M·3-M-&-M 808 PCT-AVJVJ'D>2083-M·2·M·4·M-1-M 809 PCT-A'4:1VJVF>2083-M-2·M-4-M-2-M 810 PCT-A\D\O'D>2083-M-2-M·4-M-3-M 811 PCT -A VJVJ'D>2083-M·2-M-4-M-4-M S 12 PCT ·A VJ'4:1VF>2083-M·2·M·4-M-5-M 813 PCT-A\D\O'D>2083-M-2·M-4-M-6-M 814 PCT-4VJ'4:1\1>1311-M-2·M-1·M-1-M 815 PCT-4VJVJ\1>1311-M-2-M-1 -M-2-M 816 PCT-4VJVJ\1>1311-M-2-M-1-M-3-M 818 PCT-4\D\0\1>1311-M·2·M·1·M-5-M 819 PCT-4VJVJ\1>1311·M·2·M-1-M-6·M 832 PCT-4VJVJ\1>9Q-M-2-M-5-M-1-M 833 PCT-4VJ'4:1\1>90-M-2-M·5-M·2·M 83o4 PCT-4VJ'4:1\1>90-M-2·M-5-M-3-M 835 PCT-4VJVJ\1>9Q-M·2-M·5-M-4-M 836 PCT-4VJVJ\1>90-M-2-M-5-M·5-M 837 PCT-4'4:1VJ\1>90-M-2-M·5-M-$-M 838 PCT -4VJVJ\ 1>9Q-M-2-M-&-M-1-M 839 PCT-4VJVJ\1>9Q-M-2·M-$·M-2-M 840 PCT-4'4:1'4:1\1>9Q-M-2-M-&-M-3-M 841 PCT-4VJ'4:1\1>90-M-2-M-&-M-4-M 842 PCT-4VJVJ\1>9Q-M-2·M-&-M-5-M 843 PCT-4VJVJ\1>90-M-2-M-$-M-6-M 844 PCT-4VJVJ\1>106-M-3-M· 1-M-1 -M 845 PCT -4VJVJ\ 1 >106-M-3-M-1-M-2-M 846 PCT-4'4:1'4:1\1>106-M-3-M-1-M-3-M Vg 81 81 Fl LSC BS GD N81 5 3 1 5 2 2 5 5 5 5 5 5 1 5 2 5 2 5 5 5 5 5 5 5 5 5 2 5 5 7 5 3 5 2 5 5 5 5 5 5 5 3 5 7 3 3 7 5 5 7 5 5 5 5 5 5 3 3 5 5 5 5 5 5 3 50% 72 74 75 72 74 72 72 73 66 66 67 64 64 64 62 62 62 66 62 62 63 64 85 90 63 64 63 63 62 62 64 63 62 64 63 76 82 76 n n 68 64 66 66 64 63 64 64 64 64 64 64 69 64 63 3 5 5 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Nbr. Fleld Nbr. Origln PedlgrM Vg 81 81 F1 LSc BS GD N81 254 255 256 257 258 259 260 2e1 262 263 264 265 266 267 268 269 Z70 Z71 272 Z73 Z74 Z75 276 277 va 279 280 281 282 263 264 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 19HA S9a0254 S9a025S 5980256 5980257 5980258 5980259 5980260 5980261 5980262 5980263 5980264 5980265 5980266 S9802e7 5980268 5980269 S980Z70 S980Z71 5980Z72 5980Z73 S980Z74 S980Z75 S980Z76 5980277 5980Z78 5980Z79 5980280 S980281 5980282 T1 T2 T3 5980283 5980284 5980285 5980286 5980287 5980288 5980289 5980290 5980291 5980292 5980293 5980294 5980295 5980296 5980297 5980298 5980299 5980300 5980301 19SI88 847 PCT ·4\0'D\1> 1 06-M-3-M-1-M-4-M 848 PCT-4\0'D\1>106-M-3-M-1-M-5-M 849 PCT ·4\0'D\1> 1 06-M-3-M-1-M-6-M 862 PCT ·4\0'D\1>2435-M-2-M-6-M-1-M 863 PCT-4\0'D\1>2435-M-2-M-6-M-2-M 1164 PCT-4\0'D\1>2435-M-2-M-6-M-3-M 865 PCT ·4\D'D\ 1>2435-M-2-M-6-M-2435-M-2-M-6-M-5-M 867 PCT-4\0'D\1>2435-M-2-M-6-M-6-M 868 PCT ·4\0'D\ 1>2485-M·1·M·1·M-1-M 869 PCT ·4\D\D\ 1>2485-M-1-M-1-M-2-M 870 PCT ·4\0'D\ 1>2485-M-1-M-1-M-3-M 871 PCT-4\0'D\1>2485-M-1 -M-1-M-2485-M-1 ·M-1·M-5-M 873 PCT -4\D'D\ 1>2485-M-1-M-1-M-6-M 874 PCT-4\D'D\1>2485-M-1-M-6-M-1-M 875 PCT-4\0'D\1>2485-M-1-M-6-M-2-M 876 PCT · 4\0'D\ 1 >2485-M-1-M-6-M-3-M an PCT-4\D'D\1>2485-M- 1-M-6-M~-M 878 PCT ·4\D\D\ 1>2485-M-1-M-6-M-5-M 879 PCT·4'D'D\1>2485-M·1·M-6-M-6-M aao PCT ·4\0'D\1 >2485-M-2-M-2-M-1 -M 881 PCT ·4\D\D\ 1 >2485-M-2·M·2·M·2·M 882 PCT·4\0'D\1>2485-M-2-M·2·M-3·M aa3 PCT ·4\0'D\1>2485-M-2-M-2-M~-M 884 PCT·4'D'D\1>2485-M·2-M·2·M·S·M aas PCT·4'D'D\1>2485-M-2·M·2·M-6·M 901 PCT ·4'D'D\1>2485-M-3-M·1·M--'-M 902 PCT-4\0'D\ 1>2485-M-3-M-1-M-5-M CIRA0409 O.Sabana6 O .Sabana 10 903 PCT-4'D'D\1>2485-M-3-M-1·M-6-M 904 PCT ·4\D\D\ 1>2485-M·3-M·3-M·1·M 90S PCT-4\D\D\1>2485-M·3-M·3-M·2-M 906 PCT-4\0'D\1>2485-M-3-M-3-M-3-M 907 PCT -4\0'D\1>2485-M-3-M-3-M-2485-M-3-M-3-M-S-M 909 PCT ·4\D\D\ 1>2485-M·3-M·3-M-6·M 910 PCT·4'D\D\1>2485-M·3-M·4·M-1-M 911 PCT ·4\0'D\1>2485-M-3-M-4-M-2-M 912 PCT-4\0'D\1>2485-M-3-M-4-M-3-M 913 PCT-4\D\D\1>2485-M-3-M-4-M-4-M 914 PCT·4\D'D\1>2485-M-3-M-4-M-5·M 915 PCT ·4\D'D\1>2485-M-3-M-4-M-6-M 916 PCT-4\D'D\1>2486-M-1-M-1-M-1-M 91 7 PCT·4\D'D\1>2486·M· 1·M·1·M·2·M 91 a PCT ·4\D\0\ 1>2486-M-1-M-1 -M-3-M 919 PCT·4'D'D\ 1>2486-M-1-M-1-M-4-M 920 PCT·4\D'D\1>2486-M-1 ·M-1-M·5-M 921 PCT-4\D\0\1>2486-M·1· M·1·M-6·M Vg • vigor. Bl 1 a leal blu1. 912 •leal blu~ F l a llowenng: LaC • lealeceld; BS • brown apot, NBI • neck blut: <3d • graln dlocolorallon. 3 5 5 5 3 3 5 5 5 5 5 5 5 2 5 5 3 2 3 2 3 3 3 4 3 3 3 2 3 2 3 3 3 2 3 2 3 3 2 S 2 5 2 S 3 S S 3 S 2 5 7 7 2 7 3 5 2 7 2 S 2 S 2 S 2 5 5 5 5 5 5 5 5 5 5 50'!1. 63 63 68 a7 69 64 70 69 69 70 68 69 69 68 69 80 81 81 82 81 81 79 78 80 n 80 78 73 75 66 86 90 73 74 75 73 74 74 73 74 72 73 73 74 75 74 73 73 73 73 74 3 1 5 5 S 5 5 5 5 S 5 3 5 5 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Table 11. Selected S81ines from the populations PCT-A\0\0\0> y PCT-4\0\0\1> La Libertad experimental station, 1999A Nbr. Reld Nbr. Ortgln Pedlgree Vg 81 81 A LSc BS GD N81 1999A S980011 2 S980014 3 S980017 4 S980020 5 5980021 6 S980024 7 S980026 8 S980027 9 S980041 10 S980047 11 S980053 12 S980063 13 S980064 14 S980065 15 S980068 16 S980070 17 5980075 18 5980083 19 S980085 20 S980086 21 5980093 22 S980113 23 S980125 24 S980128 25 5980131 26 S980143 27 S980144 28 S980149 29 5980150 30 5980151 31 32 33 34 35 36 37 38 39 40 41 42 43 « S980179 S980192 S980193 5980195 S980209 S980230 S980248 S980250 S980252 5980254 5980259 5980266 5980279 5980~87 19988 548 PCT·A'D'D'D>175-M+M+M·5-M 551 PCT·A \ll'D\0> 175-M+M-5-M-2-M 554 PCT·A\O'D\0>175-M-1·M-5-M·5-M 557 PCT·A\O'D\0>175-M·3-M+M·2·M 558 PCT·A'D'D'D>175-M·3-M+M·3-M 561 PCT ·A 'D'D'D> 175-M-3-M+M·S..M 563 PCT ·A \ll'D\0> 115-M-3-M-3-M-2-M 564 PCT ·A \ll'D\0> 175-M-3-M-3-M-3-M 584 PCT ·A 'D'D'D> 175-M·4-M-5-M·5-M 590 PCT·A'D'D'D> 175-M·6-M-1·M·5-M 596 PCT·A'D'D'D>189-M-2·M·2·M·5-M 606 PCT·A'D'D'D>189-M·4-M-4·M·3-M 607 PCT ·A 'D'D'D> 1 89-M·4-M·4·M·4-M 608 PCT ·A 'D'D'D> 1 89-M-4-M-4·M·5-M 611 PCT ·A\Il'D\0> 1 89-M-4-M·5-M-2·M 613 PCT·A'D'D'D>189-M-4-M-5-M·4-M 618 PCT ·A 'D'D'D> 189-M-4-M.S·M-3-M 626 PCT ·A 'D'D'D>278·M+M+M·5-M 628 PCT·A'D'D\0>278-M-1·M-2·M-1-M 629 PCT ·A 'D'D'D>278·M·1·M-2·M·2·M 636 PCT ·A 'D'D'D>394·M-1·M·1·M·3-M 656 PCT ·A 'D'D'D>394·M·1·M-6-M·5-M 668 PCT·A'D'D'D>394-M· 2·M·2·M·5-M 671 PCT·A'D'D'D>394-M·2·M-3-M-2-M 674 PCT·A'D'D'D>394-M·2·M-3-M·5-M 686 PCT·A'D'D'D>1169-M-1· M·4-M-5-M 687 PCT·A'D'D'D>1169-M·1· M·4-M·6-M 698 PCT·A'D'D'D>1321-M·2·M+M-5-M 699 PCT ·A 'D'D'D> 1321·M· 2·M·4·M·6-M 706 PCT·A'D'D'D>1485-M+M·4·M·1-M 741 760 761 763 777 810 841 8-43 845 847 864 871 884 907 PCT ·A 'D'D'D> 1488-M·5-M·1·M.s-M PCT·A'D'D'D>1674-M·6-M-6-M·1·M PCT ·A 'D'D'D> 167 4-M·6-M-6-M·2-M PCT ·A 'D'D'D> 167 4-M·6-M.S·M·4-M PCT ·A 'D'D'D> 1955-M-3-M-4-M-6-M PCT ·A 'D'D'D>2083-M-2·M-4·M-3-M PCT ·4'D'D\ 1 >90.M·2·M+M·4·M PCT ·4'D'D\1>90.M·2·M+M.S·M PCT·4'D'D\1>106-M-3-M·1·M-2·M PCT·4'D'D\1>106-M-3-M·1·M-4·M PCT·4'D'D\1>2435-M-2·M+M-3·M PCT ·4'D'D\1>2485-M·1·M·1·M·4·M PCT ·4'D'D\ 1 >2485-M·2·M·2·M·5·M PCT ·4'D'D\ 1 >2485-M-3-M-3-M-4-M 7 3 1 5 3 3 7 2 5 3 3 7 2 7 2 5 7 7 2 2 5 2 2 5 3 3 5 2 2 5 3 3 5 3 3 7 3 3 5 3 3 3 4 4 3 3 3 5 5 2 2 7 5 2 5 2 2 5 5 3 3 3 5 3 2 5 3 5 3 5 5 5 3 3 5 2 3 2 5 2 50% 64 62 63 62 63 63 70 69 72 75 85 80 82 82 82 82 81 82 80 80 85 n 82 82 81 68 66 74 62 67 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 85 3 74 68 66 73 3 63 64 64 64 3 63 3 64 3 69 80 5 74 Toble 12. EYC1Iuation of SS lines from t he population PCT -4 \ 0 \0\1>52 La libertad experimental station, 1999A Nbr. Fleld Nbr. 19WA Pedlg rM Vg 81 81 Fl LSc 85 GD N81 2 3 4 5 6 7 8 9 5980302 5980303 5980304 5980305 5980306 5980307 5980308 5980309 T1 T2 T3 5980010 10 5980011 11 5980012 12 5980013 13 5980014 14 5980015 15 5980016 16 5980317 17 5980018 18 5980319 19 5980020 20 5980021 21 5980022 22 5980023 23 5980024 24 5980325 25 5980326 26 5980327 27 5980328 28 5980029 29 5980030 30 31 32 33 34 35 36 37 38 39 40 5980331 5980332 5980333 5980034 5980335 5980336 5980337 5980338 5980339 5980340 5980041 41 5980342 42 5980343 43 5980344 44 5980345 45 5 980346 46 5980047 47 5980346 46 5980349 49 5980350 922 PCT -4'001 1>52-41 ·1-M-4-M-1-M 923 PCT -4'001 1>52-41 -1-M-4-M-2-M 924 PCT -4'0011>52-41-1-M-4-M-3-M 925 PCT -4'001 1 >52-41 -1-M-4-M-4-M 926 PCT -4'001 1>52-41·1 -M-4-M-5-M 927 PCT -4'0011>52-41 -1-M-4-M~ 928 PCT -4'001 1>52-41 -1 -M-6-M-1 -M 929 PCT -4'0011>52-41 -1-M-6-M-2-M CIRA0409 O.Sabana6 0 .5abana 10 930 PCT -4'0011>52-41 -1-M-6-M-3-M 931 932 933 934 935 PCT -4'0011>52-41-1-M~-4-M PCT -4'001 1 >52-41 -1-M-6-M-5-M PCT-4'0011>52-41 -1-M~~ PCT -4'001 1 >52-1803-3-M-3-M-1-M PCT -4'0011>52-1803·3-M-3-M-2-M 936 PCT -4'001 1>52-1803-3-M-3-M-3-M 937 PCT -4'0011 >52-1803-3-M-3-M-4-M 938 PCT -4'0011 >52-1803-3-M-3-M-5-M 939 PCT -4'001 1>52-1803-3-M-3-M-6-M 940 PCT -4'001 1 >52-1803-3-M-4-M-1-M 941 PCT -4'0011>52-1803-3-M-4-M-2-M 942 PCT -4'0011>52-1803-3-M-4-M-3-M 943 PCT -4'0011>52-1803-3-M-4-M-4-M 944 PCT -4'001 1 >52-1803-3-M-4-M-5-M 945 PCT -4'001 1>52-1803-3-M-4-M-6-M 946 PCT -4'0011>52-2324-4-M·1·M-1 -M 947 PCT -4'0011 >52·2324-4-M-1-M-2-M 946 PCT -4'001 1>52-2324-4-M-1-M-3-M 949 PCT -4'001 1 >52-2324-4-M-1 -M-4-M 950 PCT -4'001 1 >52-2324-4-M-1-M-5-M 951 952 953 954 955 956 957 958 959 960 961 PCT -4'001 1 >52-2324-4-M-1-M~ PCT -4'001 1 >52-1584-4-M-4-M-1-M PCT -4'0011 >52-1 584-4-M-4-M-2-M PCT -4'001 1 >52-1584-4-M-4-M-3-M PCT -4'0011>52-1584-4-M-4-M-4-M PCT -4'001 1 >52-1584-4-M-4-M-5-M PCT -4'001 1 >52-1584-4-M-4-M~ PCT -4'001 1 >52-1584-4-M-5-M-1-M PCT -4'001 1 >52-1584-4-M-5-M-2-M PCT -4'001 1 >52-1584-4-M-5-M-3-M PCT -4'0011>52·1584-4-M-5-M-4-M 962 PCT -4'lN11. 1 >52·1584-4-M-5-M-5-M 963 PCT -4'001 1 >52-1584-4-M-5-M-6-M 964 PCT -4'001 1>52-1584-4-M-6-M-1-M 965 PCT -4'lN11. 1 >52-1584-4-M-6-M-2-M 966 PCT -4'001 1 >52·1584-4-M-6-M-3-M 967 PCT -4'001 1 >52-1584-4-M-6-M-4-M 968 PCT -4\010\ 1>52·1584-4-M-6-M-5-M 969 PCT -4'001 1 >52·1584-4-M-6-M-6-M 970 PCT -4\010\1 >52-2197 ·3-M-3-M·1·M 3 3 3 3 3 3 3 3 5 5 3 5 2 3 3 3 3 5 5 5 5 5 5 5 2 5 2 5 5 5 5 3 3 5 5 5 5 3 5 5 5 2 5 2 5 2 5 5 5 5 5 5 5 5 3 2 5 3 2 5 2 5 2 5 2 7 2 50'J(. 69 69 68 70 69 68 69 68 63 85 88 70 71 70 68 74 73 73 73 73 73 72 71 71 73 71 72 66 68 66 66 66 68 84 83 82 82 84 80 82 82 85 84 83 83 85 84 85 78 75 74 n 3 3 3 3 5 5 5 5 3 3 3 5 3 3 3 3 3 3 3 3 3 3 3 3 3 1 3 3 3 5 3 Nbr. Fleld Nbr. Orlgln PedlgrM Vg 81 81 A LSc 8S GD N81 1999A 19988 50% 50 5980351 971 PCT -4'0.0\1 >52-2197 -3-M-3-M-2 -M 7 75 3 3 51 5980352 972 PCT -4'0.0\1 >52-2197 -3-M-3-M-3-M 7 74 3 3 52 5980353 973 PCT -4'0.0\1 >52-2197 -3-M-3-M-4-M 7 n 1 3 53 5980354 974 PCT -4'0.0\ 1 >S2-2197 -3-M-3-M-5-M 7 74 3 54 5980355 975 PCT -4'0.0\ 1 >52-2197 -3-M-3-M~ 7 78 3 55 5980356 976 PCT -4'0.0\ 1 >82-2197 -3-M~-1-M 5 78 56 5980357 9n PCT -4'0.0\ 1 >S2-2197 -3-M~-2-M 5 73 57 5980358 978 PCT -4'0.0\1>52-2197-3-M-6-M-3-M 5 75 3 58 5980359 979 PCT -4'0.0\1>82-2197-3-M-6-M-4-M 5 76 59 5980360 980 PCT -4'0.0\1 >82-2197-3-M-6-M-5-M 3 72 3 60 5980361 981 PCT -4'0.0\ 1 >52-2197 -3-M-6-M-6-M 3 75 61 5980362 982 PCT -4'0.0\ 1 >S2-1 038-1-M-4-M-1-M 5 84 62 5980363 983 PCT -4'0.0\ 1 >52-1 038-1-M-4-M-2-M 5 84 T1 CIRAD409 5 66 3 T2 O.Sabana 6 5 3 2 85 3 T3 O.Sabana 10 5 2 88 63 5980364 984 PCT -4'0.0\ 1 >S2-1 038-1-M-4-M-3-M 5 81 64 5980365 985 PCT -4'0.0\1 >52-1 038-1 -M-4-M-4-M 5 84 65 5980366 986 PCT -4'0.0\1>52-1 038-1-M-4-M-5-M 5 83 66 5980367 987 PCT -4'0.0\ 1 >52-1 038-1-M-4-M-6-M 5 83 67 5980366 988 PCT -4'0.0\ 1 >52-1 038-1 -M-6-M-1-M 5 2 83 68 5980369 989 PCT -4'0.0\1>52-1038-1-M~-2-M 5 2 85 3 1 69 5980370 990 PCT -4'0.0\1 >52-1038-1-M-6-M-3-M 5 2 83 3 3 70 5980371 991 PCT -4'0.0\ 1 >52-1 038-1-M-6-M-4-M 3 82 3 3 71 5980372 992 PCT -4'0.0\ 1 >52-1 038-1-M~-5-M 5 1 82 3 3 72 5980373 993 PCT -4'0.0\ 1 >52-1 038-1-M-6-M-6-M 5 2 83 3 Vg • vloor, 811 • leal blast; Bl 2 • leal blast; A • ftowering; LsC • leal scald; BS • brown spot; NBI • neck blast; Gd • graln óscdotallon. Table 12. Selected 58 lines from the population PCT -4 \0\0\1>52 La Libertad experimental station, 1999A Nbr. Fleld Nbr. Ortgln PedlgrM Vg 81 81 Fl LSc 8S GD N81 1999A 19988 50% 1 5980307 927 PCT -4'0.0\ 1 >52-41-1-M-4-M-6-M 3 68 2 5980309 929 PCT -4'0.0\ 1 >52-41-1-M-6-M-2-M 3 68 3 5980312 932 PCT -4'0.0\ 1 >82-41-1-M-6-M-5-M 3 70 1 1 4 5980332 952 PCT -4'0.0\ 1 >52-1584-4-M-4-M-1-M 3 84 3 3 5 5980339 959 PCT -4'0.0\ 1 >52-1584-4-M-5-M-2-M 5 82 6 5980341 961 PCT -4'0.0\ 1 >82-1584-4-M-5-M-4-M 5 84 Table 13. Evoluation of SS line.s from the population PCT -4\0\0\1>53 (Basic population. Plant selection in 53 line.s at PES in 1996 B) La Ubertad experimental station, 1999A Nbr. Fleld Nbr. Orlgln Pedlg,.. Vg Bl Bl F1 LSc as GD NBI 111fiA 1t988 50% 5980374 994 PCT -4'00.1>S3-41·2·2-3-M-1-M 5 75 3 2 5980375 995 PCT -4'00.1 >S3-41·2·2.J.M·2-M 5 75 3 3 5980378 996 PCT -4\01.0\1 >53-41·2·2-3-M.J.M 5 74 1 4 5980377 997 PCT -4'00.1>S3-41·2·2.J.M-4-M 5 75 3 5 5980378 998 PCT -4\01.0\1 >53-41·2·2-3-M-5-M 5 75 3 8 5980379 999 PCT -4'00.1 >53-41·2·2~ 5 73 7 S980380 1000 PCT -4'00.1 >53-41·2·2-4-M-1-M 5 72 8 S980381 1001 PCT -4\01.0\1>53-41·2·2-4-M-2-M 3 2 71 3 9 S980382 1002 PCT -4\01.0\1 >53-41·2·2-4-M.J.M 3 71 10 S980383 1003 PCT -4'00.1 >53-41·2-2-4-M-4-M 5 2 73 11 5980384 1004 PCT -4\01.0\1 >53-41·2·2-4-M·S-M 3 69 12 5980385 1005 PCT -4'00.1 >53-41 ·2·2-4-M-6-M 3 69 Vg • vigor; Bl 1 • leal blast; Bl 2 • leal blut; A • nowaring; LsC • leal ICald: BS • b53 La Ubertad experimental station, 1999A Nbr. Fleld Nbr. Ortgln PedlgrM Vg Bl 81 Fl LSc SS GD NBI 199SIA 1t988 50% 1 5980384 1004 PCT -4\01.0\1 >53-41·2·2-4-M-5-M 3 69 Table 14. INGER-LAC: "Vivero Internacional de Observación para America Latina• (VIOAL) para suelos ácidos Nursery for Acid soils. 1998/1999 Cons. Pedigree Vg Bl 81 AC NBI A LSc es VHB Gd Ht G.T 1 2 (50%) 1 CNA-IRAT 5\SAI0\3>127-2-M-1-M-2 3 1 2 1 1 63 1 1 1 1 104 1 2 CNA-IRAT A\SA\0\3>1-M-2-M-1-M-1 5 1 1 1 1 66 1 1 9 1 102 H 3 CNA-IRAT A\SA\0\3>1-M-2-M-1-M-2 3 1 1 1 1 66 1 1 9 1 99 H 4 CNA-IRA T A'SA\0\3> 1-M-2-M-1-M-5 3 1 1 1 1 65 1 1 9 1 108 H 5 CNA-IRA T A\SA\0\3> 1-M-2-M-2-M-1 3 1 1 1 1 63 1 1 9 1 112 H 6 CNA-IRAT A'SA\0\3>1-M-2-M-2-M-4 3 1 1 1 1 65 1 1 9 1 105 H 7 CNA·IRAT A\SA\0\3>1-M-2-M -3-M-2 3 1 1 1 1 65 1 1 9 1 112 H 8 CNA-IRAT A'SA\0\3>1·M·2·M-3-M-4 5 1 1 1 1 65 1 1 9 1 113 H 9 CNA-IRAT A\SA\0\3>1-M-2-M-3-M-5 5 1 1 1 1 65 1 1 9 1 112 H 10 CNA-IRAT A'SA\0\3>1-M-2-M-4-M-2 3 1 1 1 1 66 3 1 9 1 97 H 11 CNA-IRAT A'SA\0\3>1-M-2-M-4-M-3 1 1 1 1 1 68 3 1 9 1 95 H 12 CNA-IRAT A'SA\0\3>1-M -2-M-4-M-4 3 1 1 1 1 69 1 1 9 1 95 H 13 CNAx3608-6·1·2·2·1-M·M 3 1 2 1 1 68 1 1 9 1 112 - 14 CNAx3619-3-1-B-2-M-M 3 1 4 1 1 86 3 1 9 1 102 1 15 CNAx4754-80·B·M-7 -M-M 3 3 2 1 1 66 1 1 9 1 97 - 16 CNAx4754-128-B-M-4-M 3 1 1 1 1 74 1 1 9 1 95 H 17 CNAx4754-61 ·B·M-20-M 3 1 1 1 1 91 3 1 9 1 104 H 18 CT13480-M-16-1·M·M 3 1 1 1 1 86 3 1 3 63 - 19 CT13480-M-1 0-1-M·M 1 1 2 1 1 94 5 1 3 68 B 20 CT13480-M-9-2-M-M 1 1 1 1 1 85 3 1 3 69 1 21 CT13503-M·3·1-M·M 1 1 3 1 1 85 3 3 3 88 Bl 22 CT13503-M·13-1 -M·M 3 2 2 1 3 86 5 3 5 76 B 23 CT13503-M-18-1-M·M 3 1 1 1 1 85 5 1 5 78 1 24 CT13503-M-1 8-2-M·M 1 1 1 1 1 86 5 3 5 76 - 25 CT13503-M-18-3·M·M 1 1 1 1 1 86 5 3 5 74 - 26 CIRAD409 1 1 1 1 1 62 3 1 1 91 1 27 CIRAD410 3 3 3 1 1 67 3 S 1 87 1 28 CIRAD411 5 3 3 1 1 70 3 3 1 102 Hl 29 ORYZICA SABANA 6 3 3 4 1 1 82 5 1 1 106 1 30 ORYZICA SABANA 10 3 2 1 1 1 86 3 1 1 106 H 31 IRAT216 4 7 2 1 1 87 3 3 85 W.B S.T Amyt. (%) 0.6 L 29 0.8 L 22 1.2 L 22 2.2 L 20 2.0 L 22 1.2 L 24 1.6 L 20 1.0 L 23 1.8 L 22 1.6 L 23 1.2 L 21 1.2 L 22 0.1 EL 1.2 L 0.6 EL 1.0 L 0.4 L - - 0.6 L 1.2 L 0.8 L 0.2 L 0.6 L 1.0 L 2.0 EL 1.2 L 24 3.8 M 24 2.8 L 17 2.2 L 26 1.2 L 23 Vg • vigor. Bl 1 y 2 • leal blast: M;. acid soll reaction; A • ftowering; LSc • leal scald; BS • brown spot; NBI • neck blast; Gd • graln dlscoloraUon; Ht • helght (cm); G.T .• gelatlnlzatlon t81Tf)erature; W.B .• white belly; Nnyt. ~ amy1ose content. White Head Rice Rice (%) (%} 80.14 66.76 69.40 62.95 73.88 67.10 66.72 59.61 71 .29 63.15 65.67 58.30 67.58 58.56 70.20 63.50 71.64 65.38 72.99 65.89 72.15 67.10 74.24 67.95 1 71 .06 66.60 85.20 65.88 87.25 58.32 73.04 56.67 71 .53 61 .73 CHAPTER IV POPULATION BREEDING for LOWLAND RICE 1. INTRODUCTION The population breeding project started by introducing to Colombia, different gene pools and populations previously developed in Brazil by EMBRAPA Rice and Beans Center and CIRAD, and by CIRAD in French Guiana. The germplasm was characterized at CIA T, Palmira, and the best- adapted ones were used to develop new populations. This resulted in three populations that were registered in the recurrent selection catalog as PCT-6, PCT-7, and PCT-8. This work was conducted at CIAT in close collaboration with Drs. C. Martínez and E. P. GuimarCíes. A gene pool was also built up, using a different gene of mole sterility. The gene pool was registered as GPCT-9. A second gene pool, developed by CIRAD for temperate climates, was registered as GPIRAT-10. From late 1996, this basic germplasm was dispatched to our regional partners and outside Latín America. It is the starting point for the development of population breeding in different countries. In 1999, the II International Workshop on Rice Recurrent Selection, held in Goionia-Brazil , was the occasion for our partners to present updated information on the use of population breeding. Written documents were made available to all participants. A book is being edited by Dr. Elcio .P. GuimarCíes and will be published by CIAT, CIRAD and EMBRAPA Rice and Beans. In this report. we will onlv describe the activities developed in Colombia, by the CIAT/CIRAD project. 2. POPULATION BREEDING for VENEZUELA Two populations, PCT-6 and PCT-7, were selected as the best introduced material to be used as sources of male-sterile background to develop two new local populations, identified as PFD-1 and PFD-2. PFD-1 Male-sterile plants of PCT-6 were crossed with 5 fines: FONAIAP 1 CT 9868-3-2-3-1-4P-M-1-1P IR 62140-48-3-1-2-3 CT 9509-17 -3-1-1-M-1-3P-M-1 CT 10310-15-3-2P-4-3. PFD-2 Male-sterile plants of PCT-6 were crossed with 4 fines: CT 9868-3-2-3-1-4P-M-1-1P IR 62140-48-3-1-2-3 CT 10310-15-3-2P-4-3 CT 9509-17 -3-1-1-M-1-3P-M-1 Cropping Season 1999 At CIAT Palmira the built-up of the population PFD-2 was completed and the basic population sent to DANAC-Venezuela. 3. POPULATION BREEDING for ARGENTINA Development of local populations Population PARG-1 This germplasm is a new population with narrow genetic base, and corresponds to the mixture of the best male-sterile plants harvested in the selected progenies from fertile plants of the 3 populations PCT-6, 7, and 8. After some cycles of recombination it will be used as direct source of fertile plants for line development. Population PARG-2 This germplasm comes from the mixture of 50cyo of the population PCT- 8 \0\0\2 and 50cyo of PARG-1. Cropping season 1999 Population PARG-3 This new population was developed at CIA T by introduction of 50 ero of variabifity from 6 new fines into the population PCT-8. This new germplasm will be the starting point for recurrent selection breeding in Argentina 4. POPULA TION BREEDING for CHILE The Chilean population PQUI-1 was spfit in two parts. One sample was sown in Chillan and the other one in the northern part of the rice growing area where cfimatic conditions are different. The two populations identified as PQUI-1\Ch\0\1 and PQUI-1\Co\1\0 were sent to CIAT Palmira for completing the second cycle of recombination. In October 1998, the second cycle of recombination was shipped to Chile, but the seed transited through Miami and was incinerated by the plant protection service of the USA. Fortunately we have not shipped all the seed produced in Palmira. Santiago Hernaiz took the right decision in sowing the first cycle of recombination in order to go-on with his project. Cropping season 1999 A t CIA T Palmira, we used the remnant seeds of the second recombination to perform the third cycle during the first semester of 1999. Fixed lines were developed through anther culture at CIA T laboratory. A total of 325 R1 fines was produced. The R2 generation will be grown at CIA T Palmira and the resulting seed dispatched to Chile for evaluation during 1999/2000 cropping season. 5. POPULA TION BREEDING for URUGUAY During the 1997 -cropping season, Fernando Perez de Vida crosses different lines with sterile plants from the GPIRAT-10 gene pool. The hybrid seed was shipped to CIA T Palmira to grow the Fl. Three (3) populations are developed: PURG-1 comes from the introduction of 17 lines with short grain quality into GPIRAT-10. The objective is to develop a local population for short grain quality; witch represents a new target export market for Uruguay. PURG-2 comes from the introduction of 60 long grain lines into the gene pool GPIRAT-10. PURG-3 has a broader genetic base, and is the result of physical seed mixture of the two previous populations with male-sterile plants selected in the best progenies derived from the original gene pool GPIRAT-10. Cropping Season 1999 The build-up of the populations for Uruguay is under way at CIA T Palmira 6. MAINT AINING GERMPLASM Because we manage the catalogue for rice germplasm for recurrent selection, we also hove the responsibility to ensure the presence of sufficient seed in the germplasm bank. Because of sufficient disposability of seed, no multiplication was done this year. 7. DISTRIBUTING GERMPLASM Since 1995, we started to release recurrent selection populations and gene pools to NARS in Latín America and in West Africa and Asia. In 1999, we shipped different populations to new partners in Euro pe (Spain and France). 8. REGISTERING NEW POPULATIONS In 1999, four (4) new germplasms (see Appendix 4) were registered on request from our collaborators from Chile, China and Venezuela. • PQUI-1 Japonica population for irrigated temperate ecosystem (INIA-Quilamapu, Chile). • GPYN-2 Japonica gene pool for temperate ecosystem (FCRI/YAAS, Yunnan Province -China). • PFD-1 and PFD-2 Indica populations for tropical lowland ecosystem (DANAC- Venezuela). 9. FIXED LINE DEVELOPMENT from CHILEAN POPULATIONS, through ANTHER CULTURE To fasten the development of fixed lines for Chile where only one cropping season is possible, we decided to process through anther culture, the Chilean populations with two recurrent selection cycles. Cropping season 1998/1999 The populations PQUI-1\Ch\2\0 and PQUI-1\Co\2\0 were grown at CIA T Palmira and plants processed at the CIA T laboratory. 325 R1 lines were produced, and the R2 generation will be advanced in Colombia during the first semester of year 2000. The resulting seeds will be shipped to Chile for evaluation and selection, during the 2000/2001 cropping season. 10. FIXED LINE DEVELOPMENT from CONVENTIONAL CROSSES for ROMANIA, through ANTHER CULTURE In the framework of the collaboration between CIRAD-CA and the Romanian institution FUNDULEA, two crosses designed for cold tolerance, grain quality, and yield potential were processed by the CIAT anther culture laboratory. Cropping season 1999 Two crosses were processed: OL TENITSA 1 RUBINO, and CRISTAL /L 203. A total of 61 R1 DH lines was produced. The R2 seeds were shipped to Romanía and also to France and Chile. 11. FIXED LINE DEVELOPMENT from CONVENTIONAL CROSSES for SPAIN, through ANTHER CULTURE In the framework of the collaboration between CIRAD-CA Montpellier and Spain, four crosses were made at CIAT Palmira and will be processed by anther culture. The Fl hybrid seed was obtained, and the Fl's generations will be grown during the last trimester of 1999. In early year 2000, plants will be processed by anther culture. APPENDIX 1. CIAT RICE PROJECT IP-4 and LOGICAL FRAMEWORK Project IP-4. Improved Rice Germplasm for Latin America and the Caribbean Project Goal 1 To improve the nutritional and economic well-being of rice growers and low income consumers in Latin America and the Caribbean through sustainable increases in rice production and productivity J Project Purpose To increase rice genetic diversity and enhance gene pools for higher, more stable yields with lower unit production costs that propiciate Jower prices to consumers and reduce environmental hazards Physiological Host-Pests Interaction in Rice Project Priorities and Research Enhanced Gene Pools Basis for Rice Traits Characterized Capacities Enhanced Understood . -.. .... ------Proiect Lo2-F Narratlve Summarv Measurable lndicators Means of Verlflcatlon lm~rtant Assum~tions Goal Germplasm of beans, cassava, tropical forages, • A sufficient number of accessions (of beans, • CIArs germplasm bank rice and their wild relatives collected, conserved cassava and tropical forages) representing inventaries. and enhanced and made accessible to NARS and genetic diversity are conservad and managed other partners. ex-situ. • Partners technical reports. • Strategies and guidelines for in-situ management of biodiversity of beans, cassava • Annual reports. and tropical forages have been developed and testad with users. • Accessible germplasm of beans, cassava, tropical forages and rice meet NARS standards in terms of productivity, stability, agronomic traits and user needs. • Techniques and relevant informalion for more efficient and reliable germplasm improvement are accessible to users. Purpose T o increase rice genetic diversity and • Evaluatlons of yield potentlal (interspecific, • Databases. • Stability (interna) and externa)) enhance gene pools for higher, more stable wide, elite crosses and recurrent selection). yields with lower unit production costs that • Continuad use of improved germplasm by • Project, CIAT and NARS annual • National policies favor adoption of new propiciate lower prices to consumers and NARS. reports. technology. reduce environmental hazards. • Monitoring rice production practicas and markets. • Publications. • IPM practicas In place for stable production • Promotional Activities and cleaner environment. (conferences, training, • Rice lines selected with desired gene traits. workshops, field days) • Potential sources for high levels of biotic and abiotic stress resistance. Outputs • Pathogen/pest variation and source of resistance l. Enhanced Gene Pools. identified. 2. Physiological Basis for Rice Traits • IPM strategies. • Project progress report for • Continued support from CIA T/CIRAD/FLAR. Understood. 1998. 3. Host-Pests Interaction in Rice • Workshops. • Project progress report for 1998 . Characterized. • Training courses. • Publications. • Weed scientist in place. 4. Project Priorities and Research • Farmers' surveys. • Progress reports . Capacities Enhanced. • Continued adequate funding . • Pub\ications . • Recommendations adopted by NARS and • Project progress and workshop implemented by farmers. -- -- --· - --· ·· report_s _ - L_ ___ Narratlve Summa_ry Measurable lndlcators Means of Verlflcatlon lm_2_ortant Assur11P_tlons Activltles • Rice populations developed and improved • Project progress report for 1998. • Continued support from CIA T/CIRADIFLAR. 1.1. Rice improvement for upland and (tolerance soil acidity; resistance to blast, RHBV, T. • Field visits and evaluations in • Adequate funding and time! y release of lowland using recurrent selection and orizicolus ( 13); good grain quality; early maturity. testing sites. budget. conventíonal breeding. • Number of field trials planted and Iines selected. • Breeding populations distributed 1.2. Evaluation of savannas upland rice • Populations distributed to NARS for li ne toLAC. lines in Latin American countries. development. 1.3. Population maintenance through recombination. 1.4. Registration of new populations 1 .5. Distributíon of breedíng populations to LAC countries. 1.6. Conventional and recurrent selection breeding for hillsides upland rice. • Populations developed (14); populations in process • Project progress report for 1998. • Adequate support from IP-4. 1.7. ldentification and selection for useful (J 2); populations yield tested/molecular • Breeding populations in storage • Favorable climate. traits in wild rices with the aid of characterized (4). Partners (W ARDA, CIRAD, and field. molecular markers. lmprovement of EMBRAPA, CORNELL). • Best lines and QTL'S identified . yield potential in interspecific crosses by backcross and QTL analyses. • Number of crosses made (433); tropical irrigated • Project progress report for 1998 . 1.8. Evaluation of ínterspecifíc populations. (226), temperate (155), upland (52). Number of • Breeding populati ons in storage • Adequate funding and timely release of lntrogression of new plant type (NPT- selected lines. and lield. budget. IRRI) into LAC's gene pools. • Continued financia! support for anther culture 1.9. Use of anther and in vitro culture for • Double haploids: interspecific crosses (386 ), lab. acceleration breeding populations (815), Project progress report 1998. • Crosses, field support and operationa1 costs enhancement of gene pools. • somaclones (3758-Venezuela; 4440-Colombia) • Double haploids in storage provided by FLAR. 2.1. Weed control enhanced by the use of • Screening methods for tolerance to submergence • Project progress report for 1998 . new genotypes and practicas. developed. • Publications . • Adequate funding and management support . 2.2. ldentification of rice cultivars with • Number of genotypes with tolerance to • Weed scientist in place (IRRIICIA T) . tolerance to submergence. submergence 2.3. ldentification of rice cultivars exhibitlng • Weed competitive varieties developed high weed competition. • Yield components in NPT identified. 2.4. Characterization of useful traits in new plant type derived populations. ----- -- 3.1 . Monitoring changas in genetic structure • Virulence spectrum and genetic structure of rice • Collection of rice pathogens. • Rice crosses and populations developed by and virulence diversity of the rice blast pathogens. • Database of resistance sources breeders. pathogen. • Molecular markers associated and number of • Crosses made among resistance • Biotech. Unit identify molecular markers 3.2. Characterization and improving resistance genes. sources. associated with resistance. methods for developing durable • Sources of complete, complementary and partial • F7 lines with stable blast • Continue collaboration with FLAR. disease resistance (complete/partial resistance. resistance combining genes Pi-1 • Continue adequate funding from Colombia resistance) and Pi-2. and Rockefeller. 3 .3. Genetics and dissection of blast • Rice genome map with blast resístance genes using molecular • Rice lines with diversified resistance to RHBV resistance genes mapped . markers. and T. orizicolus. • Continue support and adequate funding from 3.4. Development and characterization of • More effective colony management. • Rice progress report for 1998. CIA T, ClRAD, and FLAR. resistance to RHBV and Tagosodes • Crop management components developed . orizicolus. • Increases capacity of NARS to screen • Continued funding from Colombia, 3.5. Develop and promote IPM strategies to germplasm. Rockefeller, Colciencias. control RHBV. • Rice progress report for 1998 . 3.6. Control of RHBV through nucleoprotein • Transgenic lines with RHBV-viral genes (187) • Publications mediated cross protection in transgenic with reduced symptoms. • Permission for field testing of transgenic rice. Transgenes introgressed into commercial plants is granted. • 3.7. Physical, chemical and molecular cultivars. • Rice progress report for 1998. • Publication and diagnostic kit characterization of the rice stripe • Characterization of RSNV and vector finished. available. • Continued support and adequate funding. necrosis virus. 3 .8. Development of RSNV diagnostic • Resistan! gennplasm selected methods and germplasm screening • Different control strategies for RSNV are under artificial conditions. techniques to implement control implemented. measures. 4 .1. Analysis of national rice samples in • Costs and coefficients of production. • Rice progress report for 1998. • Special funds continue. Colombia. • National breeding plans written. • Recommendations adopted by farmers. 4.2. Creation of a network of rice • Number of scientists trained. • Adequate funding and timely release of economics in Latín America (RECAL). • Published reports of courses . budget. 4 .3 . FLAR breeding and crop management • FLAR publications . activities in Latín America and the • Budget. Caribbean (training). 4 .4 . Promotional and diffusion of ac\ivitíes and research impact. 4 .5. FLAR lnteractions with CIRAD/CIATIIRRI. 4 .6. Collaboration with Forest Margins project for the development of ~ 1 improved upland rice cultivars for the Peruvían Selva. - - - APPENDIX 2. Individual Work Plan Individual Work Plan Project Number: IP-4 Project Tltle: lmproved Rice Germplasm for Latln Amerlca and the Carlbbean Subproject: Collaborative Rice Project between CIAT and GIRAD Starts: January 1999 Ends: December 1999 Your Name: Marc CHATEL Project Manager: Fernando Correa Project of Subproject Outputs and lndlcators Speclflc lndlcators for the outputs Output 1. Population development and improvement. Line development and use by NARS. Enhanced Gene Pools Importan! Assumptlons of the Actlvlty Level Savanna Upland Alce Continuad support from CIAT and CIRAD. Conventlonal and Recurren! Selectlon Breedlng. Adequate funding and timely ralease of budget. NARS willlng and capable to try ouVuse new improved populations. Actlvltles Semester 1 Semester 2 J•n. Feb. Mar. Apr. ~y Jun. Jul. Aug. Sep. Oct. Nov. Dec. 1.1. Recurren! Selectlon: Populatlon Enhancemenl 1.1.1. Use of Recurren! Selection for acid soüs. . .... . .... . .... . .... . .... S2 progenies evaluatlon under acld soll 1.1.2. Recombination of the best S2 progenies (Palmira) . .... . .... . .... . .... ..... ..... 2000 2000 2000 1. 1.3 Evaluation and recombination of 3 populations ..... . .... . .... . .... . .... enhanced by 3 cycles of Recurren! Selectlon for total reslstance to Blast. 1.2. Recurren! Selectlon: New Upland Savanna Populatlon. 1.2.1. Recombination cycle of population PCT -11 . .... ..... . .... . .... . .... 1.2.2. Population Enhancement for global characteristics . ..... . .... . .... . .... . .... 1.3. Recurrent Selectlon: Llne development from dlfferent populatlons. 1 .3. 1. Selectlon and evaluation of segregating llnes. ...... ..... . .... . .... . .... . .... . .... . .... 1.3.2. Off season multlpllcation (Palmlra) ..... . .... . .... 2000 2000 2000 1.4. Conventlonal Breedlng. 1.4. 1. Evaluation and selection of breedlng llnes. . .... . .... . .... . .... . .... 1.4.2. Off season multipllcation (Palmlra) . .... . .... . .... . .... . .... . .... 2000 2000 2000 1.5. Strengthenlng NARS and tralnlng. 1.5.1. Preparation of the 2d lntemational Rice Recurrent ..... ····· ..... . .... Selection Workshop ( to be held In September 1999 In Brazil) 1.5.2. Vlslts and fleld work wtth NARS. ..... . .... . .... . .... Argentina (Tucuman), Bolivia (Santa Cruz) , Venezuela (Reunillez), and Brazi (EMBRAPA Arroz e Feijao).l 1.5.3. Germplasm distribution and evaluatlon wtth Partners . .... . .... (Latín Arnerica, CIRAD, Africa, and China) 1.6. Annual Report and Publlcatlons. - Individual Work Plan Project Number: IP-4 and FLAR Project Tltle: lmproved Rice Germplasm for Latln Amerlca and the Carlbbean Subproject: Collaborative Rice Project between CIAT, GIRAD, and FLAR Starts: January 1999 Ends: December 1999 Your Name: Marc CHATEL Project Managers: Fernando Correa Luis Sanint Project of Subproject Outputs and lndlcators Speclflc lndlcatora for the outpuu Output 1. Populatlon develooment and improvement. Une development and use by NARS. Enhanced Gene Pools lmportant Assumptlons of the Actlvlty Level Tropical and Temperate Lowland Rice Continuad support from CIAT, CIRAD, and FLAR. Use of Recurrent Selectlon Breedlng. Adequate funding and timely ralease of budgel NARS willing and capable to try out/use new improved populatlons. Actlvltles Semester 1 Semester2 Jan. ~'ti). Mar. • • May Jun. Jul. Aug. SeD. Oct. 1.1. Recurrent Selectlon: Populatlon Enhancernenl 1.1.1. Purificatíon of 3 Populatíons for Hoja Blanca. . .... . .... 1.1 .2. Racombinatíon of the purifled germplasm ..... ..... . .... . .... 1.2. Recurrent Selectlon: New Troplcallowland Populatlon. 1.2.1 . Racomblnatlon of the populatlon PCT·12 . .... . .... . .... . .... . .... . .... . .... ..... . .... 1.2.2. PCT-12 Enhancement for global characteristics ..... ..... . .... . .... . .... 1.3. Recurrent Selectlon: Llne development from dlfferent populatlons. 1.3.1. Selection of SO fertile plants in different populations . .... . .... ..... . .... . .... . .... . .... 1.3.2. 51 Off season multlplicatíon (Palmira) . .... . .... . .... . .... 1.4. Strengthenlng NARS. 1.4.1. Assessment of Recurren! Selectlon progress for LAC ..... . .... ..... ..... . .... . .... . .... . .... . .... . .... Temperate and Subtroplcal Lowtands: Argentina, Chile, Uruguay and Southem Brazil 1.4.2. Assessment of Recurren! Selectlon Progress for ..... ..... . .... ..... ..... . .... . .... . .... . .... . .... Tropical Lowtands: Colombia, Venezuela, Costa Rica, El Salvador Panama and Cuba. 1.4.3. Recurren! Selectíon in Venezuela (DANAC): . .... . .... . .... . .... Definitíon of recurren! selectlon activitles In Venezuela Nov. o.c. . .... . .... . .... . .... 1.4.4. Recurren! Selection Gerrnplasm for Europa ..... . .... . .... . .... .Use of the Chilean populatlon PQUI-1 1 1.4. 5. Visits and field work with NARS ..... . .... 1.4.6. Gerrnplasm distribution and evaluation with Partners: ..... . .... . latin America and the Caribbean .CIRAD .Europa 1.5. Tralnlng 1.5.1. Preparation of the 2d lntematlonal Rice Recurren! . .... . .... ... ... ..... 1 Selection Workshop ( to be held in September 1999 in Brazil) 1 1.6. Annual Report and Publlcatlona APPENDIX 3. REFERENCES 1. Cuevas-Pérez, F.; E.P. Guimaréies; L.E. Berrio; D.I . González. 1992. Genetic base of rice in Latin America and the Caribbean, 1971 to 1989. Crop Sci. 32(4):1054- 1059. 2. Chotel, M.; E.P. Guimaréies. 1995. Recurrent selection in rice gene pools and populations: review of present status and progress. CIRAD/CIAT, Cali, Colombia. 30 p. 3. Chotel , M.; E.P. Guimaraes. 1995. Upland r ice improvement: using gene pools and populations with recessive male-sterile gene. CIRAD/CIAT, Cali , Colombia. 29 p. 4. Chotel , M.; E.P. Guimaraes. 1996. Upland r ice improvement: using gene pools and populations with recessive male-sterile gene. CIRAD/CIAT, Cali , Colombia. 31 p. 5. Chotel, M.; E.P. Guimaraes. 1995. Nomenclature system for rice gene pools, populations and recurrent selection breeding: General use and catalogue registration. CIRAD/CIAT, Cali, Colombia. 10 p. 6. Chotel , M.; E.P. Guimaréies. 1998. Catalogue registration to manage rice gene pools and populations improvement. CIRAD/CIAT, Cali , Colombia. 7. Federer, W.T. 1956. Augmented (or hoonuiaku) designs. Hawaiian Planter 's Record 55:191-208. 8. Chotel et al. CIRAD/CIAT/FLAR annual reports, 1996, 1997 and 1998. APPENDIX 4. GERMPLASM REGISTRATION 1 nstitution: Year of registration: Scientists: Ecosystem: Objectives: Germplasm type: Population development: Male-sterility source: Cytoplasm source: New variability: Crossing method: Seed mixture: Proportion mixture: Genetic constitution: Germplasm identification: Actual users: Request for seed to: PYN-1 FCRI / YAAS 1999 Dayun Tao, Fengyi Hu, Youqiong Yang, Peng Xu, and Jing Li Temperate lowland & upland For lowland: Tropical germplasm with blast resistance and fine grain quality For upland: Low temperatura tolerance and fine plant type Japonica population Synthesis of a new population. Plants of PCT -5 Polycytoplasm PCT -5 32 cultivars from Yunnan, Japan Manual crossing between sterile plants of PCT -5 and cultivars F2 seeds from each individual cross Equal for all crosses See table 1 Basic population PYN-1 \0\0\0 FCRIIY AAS, Yunnan Dayun Tao Food Crops Research lnstitute Yunnan Academy of Agricultura! Sciences Kunming 650205 The People's Republic of China Phone & fax: 86-871-5893754 E-mail: tao @ ms.kmb.ac.cn Genetic constitution of PYN-1 Parent Origin/Cross Frequency Remark (%) Li Jing No. 2 1.56 Strong cold tolerance Li Jing No. 3 1.56 Strong cold tolerance Li Jing No. 686 1.56 Strong cold tolerance Hei Xuan No. 5 1.56 Strong cold tolerance He16 76174 150-701 1 Jinglin 768 1.56 Strong cold tolerance, high yield Dian Yu No. 1 ZiNuomi 1 Keqing No. 3 1.56 High yield potential (15 T/ ha) Dian Jun No. 8 1.56 High yield, cold tolerance Xinan 175 Not clear 1.56 Wide adaptation Xiao Bei Gu Traditional from Yunnan 1.56 Strong cold tolerance San Jimang Traditional from Yunnan 1.56 Strong cold tolerance Jing Diao No. 3 Selection of Dabaigu 1.56 High yield, cold tolerance Kun Jing No. 4 Jing lin 768 1 Remei 1.56 High yield, cold tolerance Yun Jing No. 9 Selection of Xinan 175 1.56 Strong cold tolerance Yun Jing No. 23 78-220 1 BL4 1.56 High yield, cold tolerance Yun Jing No. 33 Zhannongai /4/ Chenbao No. 2 1.56 High yield, cold tolerance 11 hanzinuo 1 Fu 127(-) /// 672/716 Yun Jing No. 38 1.56 High yield, cold tolerance Yun Jing No. 135 Hongzaosheng 1 Jinglin 768 1.56 High yield, cold tolerance Yun Jing No. 136 Selection of Xinan 175 1.56 High yield, cold tolerance Cheng Bao No. 1 1.56 Blast resistance He Xi No. 2 Hongzaosheng 1 Jing hong No. 1 1.56 High yield, cold tolerance He Xi No. 4 Hongzaosheng 1 Yun Jing 135 1.56 High yield, cold tolerance He Xi No. 22-2 Xifong 1 Cu Jing No. 4 1.56 High yield He Xi No. 24 Hongzaosheng 1 Cun Jing No. 4 1.56 High yield He Xi No. 35 1.56 High yield, cold tolerance He Xi No. 39 Cu Jing No. 3 1 Yunqing No. 3 1.56 High yield He Xi No. 40 He Xi No. 15 1 Yunnen No. 15 1.56 High yield, cold tolerance He Xi No. 41 1.56 High yield, cold tolerance He Xi No. 42 He Xi No. 24 1 He Xi No. 21 1.56 High yield Dian Xi No. 1 He Xi No. 34 // Cu Jing No. 7 1 1.56 High yield, cold tolerance Cu Jing No. 17 Yunqing No. 3 1.56 High yield Cu Jing No. 22 1.56 High yield Cu Jing No. 23 Cu Jing No. 7 1 85-54 1.56 High yield PCT-5 UQiand Jagpnica Po(2ulation 50 T ro(2ical {aQ.onica, blast resistance, grain guality 1 nstitution: Year of registration: Scientists: Ecosystem: Objectives: Germplasm type: Population development: Male-sterility source: Cytoplasm source: New variability: Crossing method: Seed mixture: Proportion mixture: Genetic constitution: Germplasm identification: Actual users: Request for seed to: GPYN-2 FCAI /YAAS 1999 Dayun Tao, Fengyi Hu, Youqiong Yang, Pong Xu, and Jing Li Temperate lowland High yield, blast resistance, low temperatura tolerance, fine grain quality Japonica population Synthesis of a new derived gene pool .Piants of PCT -5 Polycytoplasm PCT -5 30 cultivars from Yunnan, Japan Manual crossing between sterile plants of PCT-5 and cultivars, then backcrossing with respect cultivars as male parents BC1 F2 seeds from each individual cross Equal for all crosses See table 2 Basic population GPYN-2\0\0\0 Yunnan Dayun Tao Food Crops Aesearch lnstitute Yunnan Academy of Agricultura! Sciences Kunming 650205 The People's Aepublic of China Phone & fax: 86-871-5893754 E-mail: tao@ ms.kmb.ac.cn Genetic constitution of GPYN-2 Parent Origin/Cross Frequency Remark (%) U Jing No. 2 2.5 Strong cold tolerance U Jing No. 3 2.5 Strong cold tolerance U Jing No. 686 2.5 Strong cold tolerance Hei Xuan No. 5 2.5 Strong cold tolerance He16 7617 4 1 50-701 1 Jinglin 768 2.5 Strong cold tolerance, high yield Dian Yu No. 1 ZiNuomi 1 Keqing No. 3 2.5 High yield potential (15 T 1 ha) Dian Jun No. 8 2.5 High yield, cold tolerance Xinan 175 Not clear 2.5 Wide adaptation Ban Jimang Traditional one from Yunnan 2.5 Strong cold tolerance Jing Diao No. 3 Selection of Dabaigu 2.5 High yield, cold tolerance Kun Jing No. 4 Jing lin 768 1 Remei 2.5 High yield, cold tolerance Yun Jing No. 9 Selection of Xinan 175 2.5 Strong cold tolerance Yun Jing No. 23 78-220 1 BL4 2.5 High yield, cold tolerance Yun Jing No. 33 Zhannongai /4/ Chenbao No. 2 2.5 High yield, cold tolerance 11 hanzinuo 1 Fu 127(-) /11 672/716 Yun Jing No. 38 2.5 High yield, cold tolerance Yun Jing No. 135 Hongzaosheng 1 Jinglin 768 2.5 High yield, cold tolerance Yun Jing No. 136 Selection of Xinan 175 2.5 High yield, cold tolerance Cheng Bao No. 1 2.5 Blast resistance He Xi No. 2 Hongzaosheng 1 Jing hong No. 1 2.5 High yield, cold tolerance He Xi No. 4 Hongzaosheng 1 Yun Jing 135 2.5 High yield, cold tolerance He Xi No. 24 Hongzaosheng 1 Cun Jing No. 4 2.5 High yield He Xi No. 35 2.5 High yield, cold tolerance He Xi No. 39 Cu Jing No. 3 1 Yunqing No. 3 2.5 High yield He Xi No. 40 He Xi No. 15 1 Yunnen No. 15 2.5 High yield, cold tolerance He Xi No. 41 2.5 High yield, cold tolerance He Xi No. 42 He Xi No. 24 1 He Xi No. 21 2.5 High yield Dian Xi No. 1 He Xi No. 34 11 Cu Jing No. 7 1 2.5 High yield, cold tolerance Cu Jing No. 17 Yunqing No. 3 2.5 High yield Cu Jing No. 22 2.5 High yield Cu Jing No. 23 Cu Jing No. 7 1 85-54 2.5 High yield PCT-5 UQiand Jag_onica PoQulation 25 TroQical [ag_onica, blast resistance, grain gualit~ 1 nstitution: Year of registration: Scientists: Ecosystem: Objectives: Germplasm type: Population development: Male-sterility source: Cytoplasm source: New variability: Crossing method: Evaluation: Seed mixture: Proportion mixture: Recombination: Genetic constitution: Germplasm identification: Actual users: Request for seed to: PFD-1 DANAC Venezuela 1999 Eduardo Graterol Tropical irrigated Adaptation to rainy conditions in Venezuela Indica population lntroduction of new variability into PCT-6 Plants of PCT -6 (population developed with male-sterile plants of IRAT MANA, which has male-sterile gene from 1 R 36 From PCT-6 5 lines of different origin Hand crossing between lines and male- sterile plants F1 's from each single cross F2 seed of all F1 plants Equal proportion of all crosses Twice on male-sterile plants without selection See table Basic recombined population PFD -1\0\0\2 DANAC Venezuela Eduardo Graterol Fundación para la Investigación Agrícola Danac Rice program Apartado Postal 182, San Felipe, Venezuela Phone: 58-54-319812 and 319565 Fax: 58-54-318512 E-mail: egratero@danac.org.ve Genetic constitution of PFD-1 Parent FONAIAP 1 CT 9868-3·2·3-1-4P·M·1-1 P CT 1031G-15-3-2P-4-3 IR 62140.48-3·1-2·3 CT 9509-17 -3·1·1-M-1·3P-M-1 PCT-6* B4353C-Kn-7..()..0-2 BG989 PNA 1 004F4-33 OR83-23 RP2087-115-1 0-5-1 Oryzlca3 Perla Oryzica Llanos 4 MorelosA88 IRATMANA ,.,. CNA1613 CNA 3814 CNA 4191 CNA4987 CNA4995 CNA 5179 CNA 5551 CNA 6158 CT 6163-8-9-1-2A IR 841-63-5-18-2 IRAT335 IRAT347 IRAT348 p 5747-12-9-3-7 #26W CNA-IRA T M *** Origin/Cross P1386·6-8M·1·3M-1/P3767 Tox 1859-12G-6M-3/P5446-8-4-1-2//P3844-F3-22·1·1 X P3083·F4-56-2·211T A306//CT8154-1-9-2 IR50401-n-2-1·3/IR36366-28·3·1·2·2 ECIA 24-1 07 -1/IR2584Q-64-1·31/CT57 46-18·11-4-1·3X Indica Population BG563/BG379-2 lntVBG90-2 C018/Hema RP1 017-76-1-4-3/Manasarovar CICA 71/CICA 81 Pe/Ita 1-1 CR1113/IRAT 122//Colomb/a 1/P 1274-6-8M C318Za76-7/C99Za76-1 Indica Population CNPAF- Brazil CNPAF-Brazil CNPAF-Brazil CNPAF-Brazil CNPAF-Braz/1 CNPAF-Brazll CNPAF-Brazll CNPAF-Braz/1 CIA T- Colombia IRRI-Ph/1/pp/nes IRA T-Braz/1 IRA T-French Gulana IRA T-French Gulana Colombia Indica Population * GENETIC CONSTITUTIUON OF PCT-6 ** GENETIC CONSTITUTIUON OF CNA-IRA T MANA ••• GENETIC CONSTITUTION OF CNA-IRA T M: Selectlon for graln shape In GPCNA-18 1 1 Frequency <1, 10.0 1· 10.0 10.0 10.0 1 10.0 50.0 1 5.55 5.55 1 5.55 5.55 1 5.55 5.55 1 5.55 5.55 l . 5.55 50.0 1 3.33 3.33 3.33 1 3.33 3.33 3.33 1 3.33 3.33 3.33 3.33 1 3.33 3.33 3.33 1 3.33 3.33 50.0 1. l . 1 1 lnstitution: Year of registration: Scientists: Ecosystem: Objectives: Germplasm type: Population development: Male-sterility source: Cytoplasm source: New variability: Crossing method: Evaluation: Seed mixture: Proportion mixture: Recombi nation: Genetic constitution: Germplasm identification: Actual users: Request for seed to: PFD-2 DANAC Venezuela 1999 Eduardo Graterol Tropical irrigated Adaptation to drought conditions in Venezuela Indica population lntroduction of new variability into PCT-7 Plants of PCT -7 (population developed with male-sterile plants of IRAT1/420P, which has male-sterile gene from IR 36 From PCT-7 4 lines of different origin Hand crossing between lines and male- sterile plants F1 's from each single cross F2 seed of all F1 plants Equal proportion of all crosses Twice on male-sterile plants without selection See table Basic recombined population PFD -2\0\0\2 DANAC Venezuela Eduardo Graterol Fundación pra la Investigación Agrícola Danac Rice program Apartado Postal 182, San Felipe, Venezuela Phone: 58-54-319812 and 319565 Fax: 58-54-318512 E-mail: egratero@danac.org.ve Genetic constitution of PFD-2 Parent CT 9868-3-2-3-1-4P-M-1-1 P CT1031Q-15-3-2P-4-3 IR621 04-48-3-1-2-3 CT9509-17 -3-1-1-M-1-3P-M-1 PCT-7* B4353C-Kn-7-0-D-2 BG989 PNA 1 004F4-33-1 OR83-23 Oryzica 3 RP2087-115-1 0-5-1 IRAT 1/420P** • Genetic constitution of PCT-7 Origin/Cross Frequency (~ T ox 1859-120-6M-3/P5446-8-4-1-2/ /P3844-F3-22-1 -1 X 12.5 P3083-F4-56-2-2/JT A306//CT8154-1-9-2 12.5 IR50401-77 -2-1-3/IR36366-28-3-1-2-2 12.5 ECIA 24-1 07 -1/IR2584o-64-1-3//CT57 46-18-11·4-1-3X 12.5 Indica Population 50.0 BG563/BG379-2 lntVBG9D-2 C018/Hema CICA 71/CICA 81 Pe/ita 1-1 RP1 O 17-76-1-4-3/Manarovar Indica Population 8.33 8.33 8.33 8.33 8.33 8.33 50.00 *"IRAT 11420P: Selectlon for earllness In CNA-IRA T 4 (GPCNA-18) 1 nstitution: Year of registration: Scientists: Ecosystem: Objectives: Germplasm type: Population development: Male-sterility source: Cytoplasm source: New variability: Crossing method: Evaluation: Seed mixture: Proportion mixture: Recombination: Genetic constitution: Germplasm identification: Actual users: Request for seed to: PQUI-1 INIA Quilamapu, Chile 1999 Santiago Hernaiz and Roberto Alvarado Temperate irrigated Adaptation to cold temperatures Japonica population lntroduction of new variability into GPIRAT-10 Plants of GPIRAT-10 (gene pool developed with male-sterility from IR 36) From GPIRAT-1 O 5 Chilean lines Hand crossing between lines and male-sterile plants of GPIRAT-10 CIA T -Colombia, F1 generation advance at CIAT, Colombia ,F2 seed of all F1 plants Different proportion of each single crosses INIA Quilamapu, Chile See table Basic recombined population PQUI -1\0\0\1 INIA Quilamapu, Chile Santiago Hernaiz and Roberto Alvarado INIA Quilamapu, Rice program Avenida Vicente Mendez 515 426 Chillan - Chile Phone: 56-42 209708 and 209701 Fax: 56-42 209599 E-mail: shernaiz@ quilamapu.inia.ch ralvarado@ quilamapu. inia.ch Genetic constitution of PQUI -1 Parent Origin/Cross Frequency (%) Diamante Chilean varlety 10.30 Bull Chilean varlety 13.30 Quila 67108 Chilean llne 16.22 CINIA609 Chilean llne 5.09 CINIA606 Chilean llne 5.09 GPIRAT-10* Japonlca Gene Pool 50.0 Anseatico -Ita/y 1.19 Bonnetbe/1 -USA 2.60 Europa -Ita/y 1.19 K oral -Ita/y 1.19 Mutique Verce/11 -Ita/y 2.60 Roce a -Ita/y 1.19 Sesia -Ita/y 1.19 Strella -Ita/y 1.19 Miara -Ita/y 14.41 6FMT Lebonnet//C/9881/IR659-1 0-8-3 1.41 IRAT 112 -Upland 1.41 L202 IR456-3-2-1-sei/72-3-2-2-7-8//L 201 1.41 Lebonnet -USA 1.41 Mercury Short Mars/Nato 1.41 Atan Labelle/L 201 1.92 La be/le Be/le Patna/Dawn 1.92 Mejanes4 -France 1.92 Rexmont USA Newrex/Bellemont 1.92 Skybonnet USAB/uebel/e//Belle Patna/Dawn 1.92 Ariete -France 2.60 Delta -Ita/y 1.19 ltalpatna -Ita/y 1.19 Lid o -Ita/y 2.60 Rica Nortai//C/9545/Nova 2.60 Senatore Novelli -Ita/y 1.19