Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus

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cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorGoogleen
cg.coverage.countryUganda
cg.coverage.iso3166-alpha2UG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970
cg.creator.identifierAlan S. Orth: 0000-0002-1735-7458
cg.creator.identifierAppolinaire Djikeng: 0000-0001-9271-3419
cg.creator.identifierStephen J Kemp: 0000-0003-4041-1720
cg.creator.identifierRobert A. Skilton: 0000-0003-4177-9404
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1186/1743-422x-9-218en
cg.isijournalISI Journalen
cg.issn1743-422Xen
cg.issue1en
cg.journalVirology Journalen
cg.reviewStatusPeer Reviewen
cg.subject.ilriAGRI-HEALTHen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriANIMAL HEALTHen
cg.subject.ilriEMERGING DISEASESen
cg.subject.ilriHEALTHen
cg.subject.ilriPIGSen
cg.subject.ilriZOONOTIC DISEASESen
cg.volume9en
dc.contributor.authorMasembe, C.en
dc.contributor.authorMichuki, George N.en
dc.contributor.authorOnyango, M.en
dc.contributor.authorRumberia, C.en
dc.contributor.authorNorling M.en
dc.contributor.authorBishop, Richard P.en
dc.contributor.authorDjikeng, Appolinaireen
dc.contributor.authorKemp, Stephen J.en
dc.contributor.authorOrth, Alan S.en
dc.contributor.authorSkilton, Robert A.en
dc.contributor.authorStahl, K.en
dc.contributor.authorFischer, A.en
dc.date.accessioned2013-03-10T10:33:26Zen
dc.date.available2013-03-10T10:33:26Zen
dc.identifier.urihttps://hdl.handle.net/10568/27684
dc.titleViral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virusen
dcterms.abstractThe rising demand for pork has resulted in a massive expansion of pig production in Uganda. This has resulted in increased contact between humans and pigs. Pigs can act as reservoirs for emerging infectious diseases. Therefore identification of potential zoonotic pathogens is important for public health surveillance. In this study, during a routine general surveillance for African swine fever, domestic pigs from Uganda were screened for the presence of RNA and DNA viruses using a high-throughput pyrosequencing method. Serum samples from 16 domestic pigs were collected from five regions in Uganda and pooled accordingly. Genomic DNA and RNA were extracted and sequenced on the 454 GS-FLX platform. Among the sequences assigned to a taxon, 53% mapped to the domestic pig (Sus scrofa). African swine fever virus, Torque teno viruses (TTVs), and porcine endogenous retroviruses were identified. Interestingly, two pools (B and C) of RNA origin had sequences that showed 98% sequence identity to Ndumu virus (NDUV). None of the reads had identity to the class Insecta indicating that these sequences were unlikely to result from contamination with mosquito nucleic acids. This is the first report of the domestic pig as a vertebrate host for Ndumu virus. NDUV had been previously isolated only from culicine mosquitoes. NDUV therefore represents a potential zoonotic pathogen, particularly given the increasing risk of human-livestock-mosquito contact.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2012-09-24
dcterms.bibliographicCitationMasembe, C., Michuki, G., Onyango, M., Rumberia, C., Norling M., Bishop, R.P., Djikeng, A., Kemp, S.J., Orth, A., Skilton, R.A., Ståhl, K. and Fischer, A. 2012. Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus. Virology Journal 9:218en
dcterms.issued2012-12
dcterms.languageen
dcterms.licenseCC-BY-2.0
dcterms.publisherSpringeren
dcterms.subjectanimal diseasesen
dcterms.subjectswineen
dcterms.subjectzoonosesen
dcterms.subjectinfectious diseasesen
dcterms.subjectvirologyen
dcterms.subjecthealthen
dcterms.typeJournal Article

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