Investigation of host range of and host defense against a mitochondrially replicating mitovirus

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cg.contributor.affiliationOkayama University
cg.contributor.affiliationRutgers University
cg.identifier.doihttps://doi.org/10.1128/jvi.01503-18
cg.issn1098-5514
cg.issue6
cg.journalJournal of Virology
cg.numbere01503-18
cg.volume93
dc.contributor.authorShahi, Sabitree
dc.contributor.authorEusebio-Cope, Ana
dc.contributor.authorKondō, Hideki
dc.contributor.authorHillman, Bradley I.
dc.contributor.authorSuzuki, Nobuhiro
dc.date.accessioned2024-12-19T12:54:13Zen
dc.date.available2024-12-19T12:54:13Zen
dc.identifier.urihttps://hdl.handle.net/10568/164712
dc.titleInvestigation of host range of and host defense against a mitochondrially replicating mitovirusen
dcterms.abstractCapsidless mitoviruses, which are ubiquitously detected in filamentous fungi, have the simplest RNA genomes of 2.2 to 4.4 kb, encoding only RNA-dependent RNA polymerase. Despite their simple genomes, detailed biological characterization of mitoviruses has been hampered by their mitochondrial location within the cell, posing challenges to their experimental introduction and study. Here we developed a protoplast fusion-based protocol for horizontal transfer of the prototype mitovirus, Cryphonectria parasitica mitovirus 1 (CpMV1), which was isolated from strain NB631 of the chestnut blight fungus ( Cryphonectria parasitica ), a model filamentous fungus for studying virus-host interactions. The host range of CpMV1 has been expanded to many different strains of C. parasitica and different fungal species within and outside the Cryphonectriaceae. Comparison of CpMV1 accumulation among various RNA silencing-deficient and -competent strains showed clearly that the virus was unaffected by RNA silencing. This study provides a solid foundation for further exploration of mitovirus-host interactions.en
dcterms.bibliographicCitationShahi, Sabitree; Eusebio-Cope, Ana; Kondo, Hideki; Hillman, Bradley I. and Suzuki, Nobuhiro. 2019. Investigation of host range of and host defense against a mitochondrially replicating mitovirus. J Virol, Volume 93, no. 6en
dcterms.issued2019-03-15
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherAmerican Society for Microbiology
dcterms.typeJournal Article

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