Characterization of a phytoplasma associated with frogskin disease in cassava

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cg.coverage.countryBrazil
cg.coverage.countryColombia
cg.coverage.countryCosta Rica
cg.coverage.countryPanama
cg.coverage.countryPeru
cg.coverage.countryVenezuela
cg.coverage.iso3166-alpha2BR
cg.coverage.iso3166-alpha2CO
cg.coverage.iso3166-alpha2CR
cg.coverage.iso3166-alpha2PA
cg.coverage.iso3166-alpha2PE
cg.coverage.iso3166-alpha2VE
cg.coverage.regionCentral America
cg.coverage.regionSouth America
cg.identifier.doihttps://doi.org/10.1094/pdis-93-11-1139
cg.isijournalISI Journal
cg.issn0191-2917
cg.issue11
cg.journalPlant Disease
cg.reviewStatusPeer Review
cg.subject.ciatCASSAVA
cg.subject.ciatMONITORING AND REPORTING
cg.subject.ciatPESTS AND DISEASES
cg.volume93
dc.contributor.authorÁlvarez, E.
dc.contributor.authorMejía de los Ríos, J.F.
dc.contributor.authorLlano Rodríguez, Germán Alberto
dc.contributor.authorLoke, John Bernard
dc.contributor.authorCalari, A
dc.contributor.authorDuduk, B
dc.contributor.authorBertaccini, A
dc.date.accessioned2014-09-24T08:41:33Zen
dc.date.available2014-09-24T08:41:33Zen
dc.identifier.urihttps://hdl.handle.net/10568/43053
dc.titleCharacterization of a phytoplasma associated with frogskin disease in cassavaen
dcterms.abstractCassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru, Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable for consumption. In Colombia, phytoplasma infections were confirmed in 35 of 39 genotypes exhibiting mild or severe CFSD symptoms either by direct or nested polymerase chain reaction (PCR) assays employing ribosomal (r)RNA operon primer pairs. The CFSD-associated phytoplasmas were identified as group 16SrIII strains by restriction fragment length polymorphism (RFLP) and sequence analyses of amplified rDNA products, and results were corroborated by PCRs employing group 16SrIII-specific rRNA gene or ribosomal protein (rp) gene primers. Collectively, RFLP analyses indicated that CFSD strains differed from all phytoplasmas described previously in group 16SrIII and, on this basis, the strains were tentatively assigned to new ribosomal and ribosomal protein subgroups 16SrIII-L and rpIII-H, respectively. This is the first molecular identification of a phytoplasma associated with CFSD in cassava in Colombia.en
dcterms.accessRightsOpen Access
dcterms.extentpp. 1139-1145
dcterms.issued2009-11
dcterms.languageen
dcterms.publisherScientific Societies
dcterms.subjectmanihot esculentaen
dcterms.subjectfrogskin diseaseen
dcterms.subjectrootsen
dcterms.subjectphytoplasmasen
dcterms.subjectpcren
dcterms.subjectametrynen
dcterms.subjectgenotypesen
dcterms.subjecttissue analysisen
dcterms.subjectenfermedad cuero de sapoen
dcterms.subjectraícesen
dcterms.subjectfitoplasmasen
dcterms.subjectgenotiposen
dcterms.subjectanálisis de tejidosen
dcterms.typeJournal Article

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