Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationChinese Academy of Agricultural Sciencesen
cg.contributor.affiliationNepal Agricultural Research Councilen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.crpLivestock and Fish
cg.coverage.regionAsia
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1038/srep29963en
cg.isijournalISI Journalen
cg.issn2045-2322en
cg.issue1en
cg.journalScientific Reportsen
cg.reviewStatusPeer Reviewen
cg.subject.ilriBREEDSen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriSHEEPen
cg.subject.ilriSMALL RUMINANTSen
cg.volume6en
dc.contributor.authorGorkhali, N.A.en
dc.contributor.authorKunzhe Dongen
dc.contributor.authorMin Yangen
dc.contributor.authorShen Songen
dc.contributor.authorKader, A.en
dc.contributor.authorShrestha, B.S.en
dc.contributor.authorXiaohong Heen
dc.contributor.authorQian-Jun Zhaoen
dc.contributor.authorYabin Puen
dc.contributor.authorXiangchen Lien
dc.contributor.authorKijas, J.en
dc.contributor.authorWeijun, Guanen
dc.contributor.authorHan Jianlinen
dc.contributor.authorLin Jiangen
dc.contributor.authorYuehui Maen
dc.date.accessioned2016-12-21T13:08:05Zen
dc.date.available2016-12-21T13:08:05Zen
dc.identifier.urihttps://hdl.handle.net/10568/78442
dc.titleGenomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayasen
dcterms.abstractSheep has successfully adapted to the extreme high-altitude Himalayan region. To identify genes underlying such adaptation, we genotyped genome-wide single nucleotide polymorphisms (SNPs) of four major sheep breeds living at different altitudes in Nepal and downloaded SNP array data from additional Asian and Middle East breeds. Using a di value-based genomic comparison between four high-altitude and eight lowland Asian breeds, we discovered the most differentiated variants at the locus of FGF-7 (Keratinocyte growth factor-7), which was previously reported as a good protective candidate for pulmonary injuries. We further found a SNP upstream of FGF-7 that appears to contribute to the divergence signature. First, the SNP occurred at an extremely conserved site. Second, the SNP showed an increasing allele frequency with the elevated altitude in Nepalese sheep. Third, the electrophoretic mobility shift assays (EMSA) analysis using human lung cancer cells revealed the allele-specific DNA-protein interactions. We thus hypothesized that FGF-7 gene potentially enhances lung function by regulating its expression level in high-altitude sheep through altering its binding of specific transcription factors. Especially, FGF-7 gene was not implicated in previous studies of other high-altitude species, suggesting a potential novel adaptive mechanism to high altitude in sheep at the Himalayas.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2016-07-22
dcterms.bibliographicCitationGorkhali, N.A., Kunzhe Dong, Min Yang, Shen Song, Kader, A., Shrestha, B.S., Xiaohong He, Qianjun Zhao, Yabin Pu, Xiangchen Li, Kijas, J., Weijun Guan, Jianlin Han, Lin Jiang and Yuehui Ma. 2016. Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas. Scientific Reports 6: 29963.en
dcterms.issued2016-07-22
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringeren
dcterms.subjectbreedsen
dcterms.subjectlivestocken
dcterms.subjectsheepen
dcterms.typeJournal Article

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