CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationPennsylvania State University
cg.contributor.affiliationSRM University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationPacific Northwest National Laboratories, USA
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorNational Science Foundation, United States
cg.contributor.donorBill & Melinda Gates Foundation
cg.coverage.countryKenya
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2KE
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.coverage.regionWestern Africa
cg.creator.identifierLeena Tripathi: 0000-0001-5723-4981
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1007/s11240-021-02210-3
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journal
cg.issn0167-6857
cg.issue1
cg.journalPlant Cell, Tissue and Organ Culture
cg.reviewStatusPeer Review
cg.subject.iitaAGRONOMY
cg.subject.iitaFOOD SECURITY
cg.subject.iitaGENETIC IMPROVEMENT
cg.subject.iitaPLANT BREEDING
cg.subject.iitaPLANT DISEASES
cg.subject.iitaPLANT HEALTH
cg.subject.iitaPLANT PRODUCTION
cg.subject.iitaVALUE CHAINS
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 1 - No poverty
cg.subject.sdgSDG 2 - Zero hunger
cg.volume150
dc.contributor.authorTrauger, M.
dc.contributor.authorHile, A.
dc.contributor.authorSreenivas, K.
dc.contributor.authorShouse, E.M.
dc.contributor.authorBhatt, J.
dc.contributor.authorLai, T.
dc.contributor.authorMohandass, R.
dc.contributor.authorTripathi, L.
dc.contributor.authorOgden, A.J.
dc.contributor.authorCurtis, W.R.
dc.date.accessioned2022-06-20T15:26:06Zen
dc.date.available2022-06-20T15:26:06Zen
dc.identifier.urihttps://hdl.handle.net/10568/119873
dc.titleCO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactoren
dcterms.abstractIn vitro plant propagation systems such as temporary immersion bioreactors (TIBs) are valuable tools that enable production of disease-free plants with improved traits. However, TIB systems can be expensive, difficult to implement, and prone to contamination due to sugar rich propagation media. Using rapidly growing chicory root cultures to expedite design-build-test cycles, we report here an improved, low-cost version of a previously reported Hydrostatically-driven TIB (Hy-TIB) that facilitates economical use of gas mixtures. Bioreactor improvements include decreased material costs, expanded modes of operation, and a horizontal orientation of a plastic film plant growth chambers that increase propagule light exposure. To take advantage of these improvements, we describe here experiments that evaluate the impacts of elevated CO2 on propagation of cacao (Theobroma cacao) secondary embryos and nodal cultures of yam (Dioscorea spp.) during both phototrophic and photomixotrophic growth. Our experiments show that elevated CO2 during plant propagation significantly improved both cacao and yam propagule development and eliminated the need for supplemental sugars in tissue culture growth media. Thus, our improved Hy-TIB shows potential as a simple, low-cost, and scalable propagation platform with cost-effective gas composition control and reduced risk of contamination overgrowth. We provide detailed instructions for assembly of this Hy-TIB design and discuss the implications of its adoption in food-insecure regions of the world.en
dcterms.accessRightsOpen Access
dcterms.audienceScientists
dcterms.available2022-04-18
dcterms.bibliographicCitationTrauger, M., Hile, A., Sreenivas, K., Shouse, E.M., Bhatt, J., Lai, T., ... & Curtis, W.R. (2022). CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor. Plant Cell, Tissue and Organ Culture, 1-15.en
dcterms.extentp. 57-71
dcterms.issued2022-07
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringer
dcterms.subjectbioreactorsen
dcterms.subjectplant propagationen
dcterms.subjecttheobroma cacaoen
dcterms.subjectdioscoreaen
dcterms.subjectfood securityen
dcterms.subjectvalue chainen
dcterms.typeJournal Article

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