Review of immunological methods for the quantification of aflatoxins in peanut and other foods

Simple item page
cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationInternational Crops Research Institute for the Semi-Arid Tropics
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.countryNiger
cg.coverage.countryIndia
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NE
cg.coverage.iso3166-alpha2IN
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionACP
cg.coverage.regionWestern Africa
cg.coverage.regionEastern Asia
cg.coverage.regionSouthern Asia
cg.howPublishedFormally Published
cg.isijournalISI Journal
cg.issn0095-3679
cg.journalPeanut Science
cg.reviewStatusPeer Review
cg.subject.iitaAFLATOXIN
cg.subject.iitaCROP HUSBANDRY
cg.subject.iitaCROP SYSTEMS
cg.subject.iitaDISEASE CONTROL
cg.subject.iitaFARM MANAGEMENT
cg.subject.iitaFOOD SECURITY
cg.subject.iitaGENETIC IMPROVEMENT
cg.subject.iitaHANDLING, TRANSPORT, STORAGE AND PROTECTION OF AGRICULTURAL PRODUCTS
cg.subject.iitaLIVELIHOODS
dc.contributor.authorWaliyar, Fariden
dc.contributor.authorReddy, S.V.en
dc.contributor.authorKumar, P. Lavaen
dc.date.accessioned2018-01-15T10:50:46Zen
dc.date.available2018-01-15T10:50:46Zen
dc.identifier.urihttps://hdl.handle.net/10568/90183
dc.titleReview of immunological methods for the quantification of aflatoxins in peanut and other foodsen
dcterms.abstractAflatoxin contamination is widespread instaple crops like peanut, maize, sorghum, pearlmillet, chillies, pistachio, cassava etc., and com-promises the safety of food and feed supplies. It isimportant to be able to detect and quantifyaflatoxins in commodities to protect human andanimal health. Many different methods, includingantibody-based ones, are available for quantita-tive estimation of aflatoxins. However, most ofthese methods such as HPLC, HPTLC, and TLCare expensive and/or difficult to use in developedcountries. Using the state-of-the-art facilities atICRISAT, we developed polyclonal and mono-clonal antibodies for the detection of totalaflatoxins, aflatoxin B1 and M1 (secreted in milk).These were used to develop a simple andinexpensive competitive enzyme-linked immuno-sorbent assays (cELISA) that has lower detectionlimits (1.0mg/kg) and cost (about $1 per sample)less than other available methods. More than 100samples can be analyzed in a day. These tests haveprovided a unique opportunity for ICRISAT andits partners to conduct field studies to selectresistant genotypes, identify high risk populationsand determine the dietary sources to stimulateappropriate interventions to enhance the food andhuman health safety, trade and thereby farmers’incomeen
dcterms.accessRightsLimited Access
dcterms.bibliographicCitationWaliyar, F., Reddy, S.V. & Kumar, P.L. (2009). Review of immunological methods for the quantification of aflatoxins in peanut and other foods. Peanut Science, 36(1), 54-59.en
dcterms.extent54-59
dcterms.issued2009
dcterms.languageen
dcterms.subjectpeanuten
dcterms.subjectarachis hypogaeaen
dcterms.subjectcompetitive elisaen
dcterms.subjectimmunological methodsen
dcterms.subjectaflatoxinsen
dcterms.subjectgenotypesen
dcterms.subjectchromatographyen
dcterms.subjectpolyclonalen
dcterms.typeJournal Article

Files

Collections

IITA Journal Articles
ICRISAT journal articles