Detection of circulating hepatitis B virus immune escape and polymerase mutants among HBV-positive patients attending Institut Pasteur de Bangui, Central African Republic

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationPan African University
cg.contributor.affiliationJomo Kenyatta University of Agriculture and Technology
cg.contributor.affiliationInternational Livestock Research Institute
cg.contributor.affiliationUniversity of Nairobi
cg.contributor.donorAfrican Union
cg.contributor.donorJapan International Cooperation Agency
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australia
cg.contributor.donorSyngenta Foundation for Sustainable Agriculture
cg.contributor.donorBill & Melinda Gates Foundation
cg.contributor.donorDepartment for International Development, United Kingdom
cg.contributor.donorSwedish International Development Cooperation Agency
cg.coverage.countryCentral African Republic
cg.coverage.iso3166-alpha2CF
cg.coverage.regionAfrica
cg.coverage.regionMiddle Africa
cg.creator.identifierJohn Juma: 0000-0002-1481-5337
cg.creator.identifierRoger Pelle: 0000-0003-1053-085X
cg.creator.identifierEunice M. Machuka: 0000-0002-1142-3442
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1016/j.ijid.2019.10.039
cg.isijournalISI Journal
cg.issn1878-3511
cg.journalInternational Journal of Infectious Diseases
cg.reviewStatusPeer Review
cg.subject.ilriDISEASE CONTROL
cg.subject.ilriHUMAN HEALTH
cg.volume90
dc.contributor.authorKoyaweda, G.W.
dc.contributor.authorOngus, J.R.
dc.contributor.authorMachuka, Eunice M.
dc.contributor.authorJuma, John
dc.contributor.authorMacharia, R.
dc.contributor.authorKomas, N.P.
dc.contributor.authorPelle, Roger
dc.date.accessioned2020-08-20T19:11:58Zen
dc.date.available2020-08-20T19:11:58Zen
dc.identifier.urihttps://hdl.handle.net/10568/109046
dc.titleDetection of circulating hepatitis B virus immune escape and polymerase mutants among HBV-positive patients attending Institut Pasteur de Bangui, Central African Republicen
dcterms.abstractPrevious studies in the Central African Republic (CAR) have reported the presence of hepatitis B virus (HBV) recombinant genotype E/D and a suspicion of immune escape mutants (IEMs), without further investigation into their impact on prevention and diagnosis. Consequently, this study investigated HBV mutations among hepatitis B surface antigen (HBsAg)-positive patients attending Institut Pasteur de Bangui in the CAR.Sera from a total of 118 HBsAg-positive patients with no previous history of HBV treatment or vaccination at the Institut Pasteur de Bangui, were sampled between 2017 and 2019. Subsequently, the region spanning the surface and polymerase genes of HBV was amplified by PCR and sequenced. HBV sequences were genotyped/subgenotyped by phylogenetic analysis and serotyped based on predicted amino acid residues at positions s122, s127, s140, s159, and s160. They were then analyzed for HBV IEMs and polymerase mutations.The region spanning the surface and polymerase genes was successfully amplified and sequenced for 51 samples. Of the HBV sequences, 49 were genotype E and two were genotype A subgenotype A1; these were serotyped as ayw4 and ayw1, respectively. Potential IEMs sY100C, sA128V, and sM133T, and several polymerase mutants were identified.This study raises awareness of the need for further studies to be conducted on a large scale to better understand HBV mutations for improved disease control and prevention strategies in the country.en
dcterms.accessRightsOpen Access
dcterms.audienceScientists
dcterms.bibliographicCitationKoyaweda, G.W., Ongus, J.R., Machuka, E., Juma, J., Macharia, R., Komas, N.P. and Pelle, R. 2020. Detection of circulating hepatitis B virus immune escape and polymerase mutants among HBV-positive patients attending Institut Pasteur de Bangui, Central African Republic. International Journal of Infectious Diseases 90:138-144.en
dcterms.extentp. 138-144
dcterms.issued2020-01
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherElsevier
dcterms.subjecthepatitisen
dcterms.subjecthealthen
dcterms.subjectdisease controlen
dcterms.typeJournal Article

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