Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells

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cg.contributor.affiliationInternational Livestock Research Institute
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169
cg.creator.identifierTushaar Shah: 0000-0002-0565-8464
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970
cg.creator.identifierEtienne de Villiers: 0000-0001-9878-1009
cg.creator.identifierRobert A. Skilton: 0000-0003-4177-9404
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1111/j.1365-2583.2005.00571.x
cg.issn0962-1075
cg.issn1365-2583
cg.issue4
cg.journalInsect Molecular Biology
cg.subject.ilriPESTS
cg.subject.ilriGENETICS
cg.volume14
dc.contributor.authorLambson, B.
dc.contributor.authorNene, Vishvanath M.
dc.contributor.authorObura, M.
dc.contributor.authorShah, Tushaar
dc.contributor.authorPandit, P.
dc.contributor.authorOle-MoiYoi, O.K.
dc.contributor.authorDelroux, K.
dc.contributor.authorWelburn, S.C.
dc.contributor.authorSkilton, Robert A.
dc.contributor.authorVilliers, Etienne P. de
dc.contributor.authorBishop, Richard P.
dc.date.accessioned2012-07-09T05:47:05Zen
dc.date.available2012-07-09T05:47:05Zen
dc.identifier.urihttps://hdl.handle.net/10568/21174
dc.titleIdentification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cellsen
dcterms.abstractIxodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs had significant identity (< 1e-15) to previously identified sequences. Polypeptides that may interact with host pathways included a kinase inhibitor. Two proteins encoded homologues of the yolk protein vitellogenin and seventeen contained glycine-rich motifs. Four proteins without sequence matches had conserved structural folds, identified using a Threading algorithm. Predicted secretion signals were between fifteen and fifty-seven amino acids long. Four homologous polymorphic proteins contained conserved (26/27 residues) signal peptides. Ten functional tick secretion signals could not be unambiguously identified using predictive algorithms.en
dcterms.accessRightsLimited Access
dcterms.audienceScientists
dcterms.available2005-07-15
dcterms.bibliographicCitationLambson, B., Nene, V., Obura, M., Shah, T., Pandit, P., Ole‐MoiYoi, O., Delroux, K., Welburn, S., Skilton, R., De Villiers, E., & Bishop, R. (2005). Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells. Insect Molecular Biology, 14(4), 403–414. Portico. https://doi.org/10.1111/j.1365-2583.2005.00571.xen
dcterms.extentp. 403-414
dcterms.issued2005-08
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWiley
dcterms.replaceshttps://hdl.handle.net/10568/32949
dcterms.subjectinsectsen
dcterms.subjectticksen
dcterms.subjectixodidaeen
dcterms.subjectmetastigmataen
dcterms.subjectsalivary glands [exocrine glands]en
dcterms.subjectsecretionen
dcterms.subjectmammalsen
dcterms.subjectcellsen
dcterms.subjectdnaen
dcterms.subjectrhipicephalus appendiculatusen
dcterms.subjectnucleotide sequenceen
dcterms.subjectamblyomma variegatumen
dcterms.subjectpolymorphismen
dcterms.subjectproteinsen
dcterms.typeJournal Article

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