Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya

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cg.contributor.affiliationUniversity of Nairobi
cg.contributor.affiliationInternational Livestock Research Institute
cg.contributor.crpAgriculture for Nutrition and Health
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierRobert A. Skilton: 0000-0003-4177-9404
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1007/s11250-012-0202-5
cg.isijournalISI Journal
cg.issn0049-4747
cg.issn1573-7438
cg.issue1
cg.journalTropical Animal Health and Production
cg.reviewStatusPeer Review
cg.subject.ilriAGRI-HEALTH
cg.subject.ilriDAIRYING
cg.subject.ilriEMERGING DISEASES
cg.subject.ilriFARMING SYSTEMS
cg.subject.ilriLIVESTOCK
cg.subject.ilriZOONOTIC DISEASES
cg.volume44
dc.contributor.authorKang'ethe, Erastus K.
dc.contributor.authorMulinge, E.K.
dc.contributor.authorSkilton, Robert A.
dc.contributor.authorNjahira, Moses N.
dc.contributor.authorMonda, J.G.
dc.contributor.authorNyongesa, C.
dc.contributor.authorMbae, C.K.
dc.contributor.authorKamwati, S.K.
dc.date.accessioned2012-09-29T06:02:37Zen
dc.date.available2012-09-29T06:02:37Zen
dc.identifier.urihttps://hdl.handle.net/10568/24412
dc.titleCryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenyaen
dcterms.abstractA total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl–Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twenty-five samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans.en
dcterms.accessRightsLimited Access
dcterms.audienceScientists
dcterms.available2012-07-15
dcterms.bibliographicCitationKang’ethe, E.K., Mulinge, E.K., Skilton, R.A., Njahira, M., Monda, J.G., Nyongesa, C., Mbae, C.K. and Kamwati, S.K. 2012. Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya. Tropical Animal Health and Production 44(1): 25-31en
dcterms.extentp. 25-31
dcterms.issued2012-09
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherSpringer
dcterms.subjectdairiesen
dcterms.subjectfarming systemsen
dcterms.subjectlivestocken
dcterms.subjectzoonosesen
dcterms.typeJournal Article

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