DNA fingerprinting reveals varietal composition of Vietnamese cassava germplasm (Manihot esculenta Crantz) from farmers’ field and genebank collections

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cg.authorship.typesCGIAR and developing country institute
cg.authorship.typesCGIAR and advanced research institute
cg.contributor.affiliationInternational Center for Tropical Agriculture
cg.contributor.affiliationNational University of Colombia
cg.contributor.affiliationAgricultural Genetics Institute, Vietnam
cg.contributor.affiliationInstitute of Agricultural Sciences for Southern Vietnam
cg.contributor.crpRoots, Tubers and Bananas
cg.coverage.countryVietnam
cg.coverage.iso3166-alpha2VN
cg.coverage.regionSouth-eastern Asia
cg.creator.identifierTatiana Ovalle: 0000-0002-5439-5461
cg.creator.identifierJohn Ocampo: 0000-0002-2720-7824
cg.creator.identifierRicardo Labarta: 0000-0003-3517-8768
cg.creator.identifierLuis Augusto Becerra Lopez-Lavalle: 0000-0003-3520-2270
cg.identifier.doihttps://doi.org/10.1007/s11103-021-01124-0
cg.isijournalISI Journal
cg.issn1573-5028
cg.issue3
cg.journalPlant Molecular Biology
cg.reviewStatusPeer Review
cg.subject.alliancebiovciatCASSAVA
cg.subject.alliancebiovciatPLANT BREEDING
cg.subject.impactAreaNutrition, health and food security
cg.subject.impactAreaPoverty reduction, livelihoods and jobs
cg.subject.sdgSDG 1 - No poverty
cg.subject.sdgSDG 2 - Zero hunger
cg.volume109
dc.contributor.authorOcampo, John
dc.contributor.authorOvalle, Tatiana
dc.contributor.authorLabarta, Ricardo Antonio
dc.contributor.authorPhuong Le, Dung
dc.contributor.authorHaan, Stefan de
dc.contributor.authorVu, Nguyen Anh
dc.contributor.authorKha, Le Quy
dc.contributor.authorBecerra López Lavelle, Luis Augusto
dc.date.accessioned2022-03-15T16:26:15Zen
dc.date.available2022-03-15T16:26:15Zen
dc.identifier.urihttps://hdl.handle.net/10568/118382
dc.titleDNA fingerprinting reveals varietal composition of Vietnamese cassava germplasm (Manihot esculenta Crantz) from farmers’ field and genebank collectionsen
dcterms.abstractCassava is the most important smallholder cash crops in Southeast Asia and is especially used in industrial products. Yet, systematic genetic studies on molecular markers from Vietnamese germplasm have not been considered for breeding and conservation programs. We conducted a molecular analysis of 1570 clones of cassava germplasm from farms across six agro-ecological zones using informative SNP markers. We unraveled the genetic diversity and population structure and provided insights into the value of breeding and conservation programs. Duplicated genotypes comprised 98% of the total sample of the Central Highlands region. Ninety-six SNPs were amplified Central Highlands and South East provinces had the highest allelic richness, covering up to 83% of alleles. The average observed heterozygosity (Ho = 0.43) was slightly higher than expected (He = 0.40) across SNP markers, suggesting an excess of heterozygotes plants. Diversity indexes indicated that cassava populations from North West and Eastern Vietnam are genetically diverse (mean He = 0.40). Genetic parentage tests identified 85 unique genetic groups within the varieties KM94, KM419, BRA1305, KM101, KM140, PER262, KM60, KM57 and two unidentified varieties, which accounted for 82% of the frequency distribution. KM94 is the most dominant variety in Vietnamese farms surveyed (38%), reflecting its superior quality and productivity. Discriminant analysis of principal components (DAPC) revealed four main subgroups, which were partially corroborated by neighbor joining (NJ) analyses. After removing duplicates, 31 unique genotypes were distributed across five of the agro-ecological zones. These were well distributed in the subgroups revealed via DAPC and NJ analyses. The genetic groups identified herein could be used to select unique accessions that should ideally conform with ex situ germplasm collections and identify areas where on-farm conservation programs should be targeted. Newly identified genotypes may also contribute as genetic breeding resources that could be used to adapt cassava to future changes and farmers’ needs.en
dcterms.accessRightsOpen Access
dcterms.available2021-02-25
dcterms.bibliographicCitationOcampo, J.; Ovalle, T.; Labarta, R.; Phuong Le, D.; de Haan, S.; Vu, N.A.; Kha, L.Q.; Becerra Lopez-Lavalle, L.A. (2021) DNA fingerprinting reveals varietal composition of Vietnamese cassava germplasm (Manihot esculenta Crantz) from farmers’ field and genebank collections. Plant Molecular Biology, Online first paper (25 February 2021). 18 p. ISSN: 1573-5028en
dcterms.extentp. 215-232
dcterms.issued2022-06
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringer
dcterms.subjectsingle nucleotide polymorphismen
dcterms.subjectgenetic markersen
dcterms.subjectgenetic variationen
dcterms.subjectcassavaen
dcterms.subjectgermplasmen
dcterms.subjectgenotypesen
dcterms.subjectpolimorfismo de un solo nucleótidoen
dcterms.subjectmarcadores genéticosen
dcterms.subjectvariación genéticaen
dcterms.subjectmanihot esculentaen
dcterms.typeJournal Article

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