Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin

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cg.authorship.typesCGIAR and developing country institute
cg.contributor.affiliationEcole Normale Supérieure de Natitingou
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversité de Montpellier
cg.contributor.affiliationMinistère de la Santé, Bénin
cg.contributor.affiliationUniversité d'Abomey-Calavi
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.donorInstitut de Recherche pour le Developpement, France
cg.coverage.countryBenin
cg.coverage.iso3166-alpha2BJ
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.creator.identifierRousseau Djouaka: 0000-0003-4772-0753
cg.creator.identifierGenevieve Tchigossou: 0000-0001-6626-8489
cg.creator.identifierINNOCENT DJEGBE: 0000-0001-6919-768X
cg.howPublishedFormally Published
cg.identifier.doihttps://doi.org/10.1186/s12879-024-09440-8
cg.identifier.iitathemeNUTRITION & HUMAN HEALTH
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journal
cg.issn1471-2334
cg.issue1
cg.journalBMC Infectious Diseases
cg.number545
cg.reviewStatusPeer Review
cg.subject.actionAreaSystems Transformation
cg.subject.iitaDISEASE CONTROL
cg.subject.iitaPESTS OF PLANTS
cg.subject.iitaPLANT DISEASES
cg.subject.impactAreaNutrition, health and food security
cg.subject.impactPlatformNutrition, Health and Food Security
cg.subject.sdgSDG 2 - Zero hunger
cg.volume24
dc.contributor.authorDjegbe, I.
dc.contributor.authorHessou-Djossou, D.
dc.contributor.authorBoukari, M.Y.G.
dc.contributor.authorNonfodji, O.
dc.contributor.authorTchigossou, G.
dc.contributor.authorDjouaka, R.
dc.contributor.authorCornelie, S.
dc.contributor.authorAkogbeto, M.
dc.contributor.authorDjogbenou, S.L.
dc.contributor.authorChandre, F.
dc.date.accessioned2024-11-25T10:44:29Zen
dc.date.available2024-11-25T10:44:29Zen
dc.identifier.urihttps://hdl.handle.net/10568/162697
dc.titlePhysico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Beninen
dcterms.abstractBackground This study aimed to investigate the relationship between the physicochemical characteristics of An. gambiae s.s. and An. coluzzii breeding sites, the susceptibility profiles to commonly used insecticides in public health, and the underlying insecticide resistance mechanisms. Methods Anopheles breeding sites surveys were conducted in Cotonou and Natitingou in September 2020, January and August 2021. Physicochemical properties and bacterial loads were determined in individual breeding sites. The WHO susceptibility assays were carried out using the female of the emerging adult mosquitoes. Anopheles species were identified through PCR techniques. Kdr L1014F/S, N1575Y and G119S mutations were investigated using TaqMan genotyping assays. Results Molecular analysis showed that all mosquitoes analyzed in Cotonou were Anopheles coluzzii, while those of Natitingou were Anopheles gambiae s.s. Fecal coliforms were identified as playing a role in this distribution through their significant influence on the presence of An. coluzzii larvae. WHO susceptibility assay indicated a high level of resistance to deltamethrin in the two cities. The resistance levels to deltamethrin were higher in Cotonou (X2 = 31.689; DF = 1; P < 0.0001). There was a suspected resistance to bendiocarb in Cotonou, whereas the mosquito population in Natitingou was resistant. The kdr L1014F mutation was highly observed in both mosquito populations (frequence: 86–91%), while the Ace-1 mutation was found in a small proportion of mosquitoes. In Cotonou, salinity was the only recorded physicochemical parameter that significantly correlated with the resistance of Anopheles mosquitoes to deltamethrin (P < 0.05). In Natitingou, significant correlations were observed between the allelic frequencies of the kdr L1014F mutation and pH, conductivity, and TDS. Conclusion These results indicate a high level of pyrethroid resistance in the anopheles populations of both Cotonou and Natitingou. Moreover, this study report the involvement of abiotic factors influencing Anopheles susceptibility profile.en
dcterms.accessRightsOpen Access
dcterms.audienceScientists
dcterms.available2024-05-30
dcterms.bibliographicCitationDjègbè, I., Hessou-Djossou, D., Gounou Boukari, M.K.Y., Nonfodji, O., Tchigossou, G., Djouaka, R., ... & Chandre, F. (2024). Physico-chemical characterization of Anopheles gambiae sl breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin. BMC Infectious Diseases, 24(1): 545, 1-12.en
dcterms.extent1-12
dcterms.issued2024
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringer
dcterms.replaceshttps://hdl.handle.net/10568/163349
dcterms.subjectmalariaen
dcterms.subjectanopheles gambiaeen
dcterms.subjectbreedingen
dcterms.subjectchemicophysical propertiesen
dcterms.typeJournal Article

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