AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis

Simple item page
cg.coverage.countryColombia
cg.coverage.iso3166-alpha2CO
cg.coverage.regionAfrica
cg.coverage.regionSouth America
cg.creator.identifierJoe Tohme: 0000-0003-2765-7101
cg.identifier.urlhttp://mic.sgmjournals.org/cgi/reprint/145/1/107en
cg.isijournalISI Journalen
cg.issn1465-2080en
cg.journalMicrobiologyen
cg.reviewStatusPeer Reviewen
cg.subject.ciatBIODIVERSITYen
cg.subject.ciatCASSAVAen
cg.subject.ciatPESTS AND DISEASESen
dc.contributor.authorRestrepo, S.en
dc.contributor.authorDuque E., Myriam Cristinaen
dc.contributor.authorTohme, Joseph M.en
dc.contributor.authorVerdier, Valerie M.en
dc.date.accessioned2014-09-24T07:58:12Zen
dc.date.available2014-09-24T07:58:12Zen
dc.identifier.urihttps://hdl.handle.net/10568/42558
dc.titleAFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotisen
dcterms.abstractSummary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease management strategy, the genetic diversity of the pathogen's populations must be assessed. Until now, the genetic diversity of Xam was characterized by RFLP analyses using ribotyping, and plasmid and genomic Xam probes. We used AFLP (amplified fragment length polymorphism), a novel PCR-based technique, to characterize the genetic diversity of Colombian Xam isolates. Six Xam strains were tested with 65 AFLP primer combinations to identify the best selective primers. Eight primer combinations were selected according to their reproducibility, number of polymorphic bands and polymorphism detected between Xam strains. Forty-seven Xam strains, originating from different Colombian ecozones, were analysed with the selected combinations. Results obtained with AFLP are consistent with those obtained with RFLP, using plasmid DNA as a probe. Some primer combinations differentiated Xam strains that were not distinguished by RFLP analyses, thus AFLP fingerprinting allowed a better definition of the genetic relationships between Xam strains.en
dcterms.accessRightsOpen Access
dcterms.issued1999
dcterms.languageen
dcterms.subjectmanihot esculentaen
dcterms.subjectxanthomonas axonopodisen
dcterms.subjectgenetic markersen
dcterms.subjectdna fingerprintingen
dcterms.subjectdnaen
dcterms.subjectgenetic variationen
dcterms.subjectmarcadores genéticosen
dcterms.subjecthuellas genéticas adnen
dcterms.subjectadnen
dcterms.subjectvariación genéticaen
dcterms.typeJournal Article

Files

Collections

CIAT Articles in Journals